Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
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Effects of Maternal Folic Acid Nutritional Status on the Expression of Myelin Basic Protein in the Offspring

어미 쥐의 엽산 영양상태가 자손 쥐의 수초기본단백질(Myelin Basic Protein)발현에 미치는 영향

  • Chae, Eun-Hye (Department of Nutritional Sciences, Ewha Womans University) ;
  • Kim, Soo-Jung (Department of Nutritional Sciences, Ewha Womans University) ;
  • Lee, Hwa-Young (Department of Anatomy, Ewha Womans Medical Center) ;
  • Chang, Nam-Soo (Department of Nutritional Sciences, Ewha Womans University)
  • 채은혜 (이화여자대학교 생활환경대학 식품영양학과) ;
  • 김수정 (이화여자대학교 생활환경대학 식품영양학과) ;
  • 이화영 (이화여자대학교 의과대학 해부학교실) ;
  • 장남수 (이화여자대학교 생활환경대학 식품영양학과)
  • Published : 2007.03.31
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Abstract

Myelin basic protein (MBP), a major structural protein of the myelin, is thought to be important for the maintenance of myelin in the central nervous system (CNS). We investigated the effect of maternal folic acid nutritional status on the folate level and the synthesis of MBP in the offspring. In order to test this hypothesis, female Sprague-Dawley rats were fed either folic acid sufficient (8 mg/kg diet) or deficient (0 mg/kg diet) diet from 2 wks prior to the mating throughout the entire pregnancy, lactation and weaning period. We examined plasma folate level by the radioimmunoassay and homocysteine level by HPLC, respectively. The MBP expression was measured by the western blot analysis. The maternal folic acid deficiency decreased plasma folate level with a concomitant increase in plasma homocysteine level in their offspring. The maternal folic acid deficiency decreased hepatic levels of SAM and SAM/SAH ratio with a concomitant increase in hepatic levels of SAH and the MBP expression of spinal cord in their offspring at 7 wks of age. These results suggest that maternal folic acid nutritional status affect plasma folate and homocysteine level in their offspring. Moreover, the maternal folic acid deficiency mi호t inhibit the MBP expression of the spinal cord and disrupt many other vital CNS reactions in their offspring.

Keywords

References

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