Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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In vitro and Lactation Responses in Mid-lactating Dairy Cows Fed Protected Amino Acids and Fat

  • Nam, I.S. (Department of Animal Life and Environment Science, Hankyong National University) ;
  • Choi, J.H. (Department of Animal Life and Environment Science, Hankyong National University) ;
  • Seo, K.M. (Department of Animal Life and Environment Science, Hankyong National University) ;
  • Ahn, J.H. (Department of Animal Life and Environment Science, Hankyong National University)
  • Received : 2014.02.07
  • Accepted : 2014.07.08
  • Published : 2014.12.01
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Abstract

The objective of this study was to evaluate the effect of ruminally protected amino acids (RPAAs) and ruminally protected fat (RPF) supplementation on ruminal fermentation characteristics (in vitro) and milk yield and milk composition (in vivo). Fourteen mid-lactating Holstein dairy cows (mean weight $653{\pm}62.59kg$) were divided into two groups according to mean milk yield and number of days of postpartum. The cows were then fed a basal diet during adaptation (2 wk) and experimental diets during the treatment period (6 wk). Dietary treatments were i) a basal diet (control) and ii) basal diet containing 50 g of RPAAs (lysine and methionine, 3:1 ratio) and 50 g of RPF. In rumen fermentation trail (in vitro), RPAAs and RPF supplementation had no influence on the ruminal pH, dry matter digestibility, total volatile fatty acid production and ammonia-N concentration. In feeding trial (in vivo), milk yield (p<0.001), 4% fat corrected milk (p<0.05), milk fat (p<0.05), milk protein (p<0.001), and milk urea nitrogen (p<0.05) were greater in cows fed RPAAs and RPF than the corresponding values in the control group. With an index against as 0%, the rates of decrease in milk yield and milk protein were lower in RPAAs and RPF treated diet than those of basal diet group (p<0.05). In conclusion, diet supplemented with RPAAs and RPF can improve milk yield and milk composition without negatively affecting ruminal functions in Holstein dairy cows at mid-lactating.

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References

  1. AOAC. 1984. Official Method of Analysis (14th Ed.). Association of Official Analytical Chemists. Washington, DC, USA.
  2. Bines, J. A., P. E. Brumby, J. E. Storry, R. J. Fulford, and G. J. Braithwaite. 1978. The effect of protected lipids on nutrient intake, blood and rumen metabolites and milk secretion in dairy cows during early lactation. J. Agric. Sci. 91:135-150. https://doi.org/10.1017/S0021859600056690
  3. Boggs, D. L., W. G. Bergen, and D. R. Hawkins. 1987. Effects of tallow supplementation and protein withdrawal on ruminal fermentation, microbial synthesis and site of digestion. J. Anim. Sci. 64:907-914. https://doi.org/10.2527/jas1987.643907x
  4. Briggs, P. K., J. P. Hogen, and R. L. Reid. 1957. Effect of volatile fatty acid, lactic acid and ammonia on rumen pH in sheep. Aust. J. Agric. Res. 8:674-690. https://doi.org/10.1071/AR9570674
  5. Canale, C. J., L. D. Muller, H. A. McCahon, T. J. Whitsel, G. A. Varga, and M. J. Lormore. 1990. Dietary fat and ruminally protected amino acids for high producing dairy cows. J. Dairy Sci. 73:135-141. https://doi.org/10.3168/jds.S0022-0302(90)78656-0
  6. Chalupa, W., B. Vecchiarelli, A. E. Elser, D. S. Kronfeld, D. Sklan, and D. L. Palmquist. 1986. Rumenal fermentation in vivo as influenced by long chain fatty acids. J. Dairy Sci. 69:1293-1301. https://doi.org/10.3168/jds.S0022-0302(86)80535-5
  7. Chaney, A. L. and E. P. Marbach. 1962. Modified reagent for determination of urea and ammonia. Clin. Chem. 8:130-132.
  8. Chapoutot, P., P. Schmidely, D. Sauvant, J. C. Robert, and B. Sloan. 1992. Influence of a ruminally protected blend of methionine and lysine on the dairy cow nutrition and production. J. Dairy Sci. 75(Suppl. 1):199(abstr.).
  9. Depeters, E. J. and J. P. Cant. 1992. Nutritional factors influencing the nitrogen composition of milk: A review. J. Dairy Sci. 72:2043-2070.
  10. Donkin, S. S., G. A. Varga, T. F. Sweeney, and L. D. Muller. 1989. Rumen protected methionine and lysine: Effects on animal performance, milk protein yield and physiological measure. J. Dairy Sci. 72:1484-1491. https://doi.org/10.3168/jds.S0022-0302(89)79258-4
  11. Fuller, M. F., R. McWilliam, T. C. Wang, and L. R. Giles. 1989. The optimum dietary amino acid pattern for growing pigs. Br. J. Nutr. 62:255-267. https://doi.org/10.1079/BJN19890028
  12. Goering, H. K. and Van Soest. 1980. Forage Fiber Analysis. (Apparatus, Reagents, Procedures and Some Application). Agricultural Hand Book. No. 379, ARS-USDA, Washington, DC, USA.
  13. Hill, G. M. and J. W. West. 1991. Rumen protected fat in kline barley or corn diets for beef cattle: Digestion, physiological, and feedlot responses. J. Anim. Sci. 69:3376-3388. https://doi.org/10.2527/1991.6983376x
  14. Kumar, S., S. S. Dagar, S. K. Sirohi, R. C. Padhyay, and A. K. Puniya. 2013. Microbial profiles, in vitro gas production and dry matter digestibility based on various rations of roughage to concentrate. Ann. Microbiol. 63:541-545. https://doi.org/10.1007/s13213-012-0501-0
  15. Izumi, K., C. Kikuchi, and M. Okamoto. 2000. Effect of rumen protected methionine on lactational performance of dairy cows. Asian Australas. J. Anim. Sci. 13:1235-1238. https://doi.org/10.5713/ajas.2000.1235
  16. Lee, J. M., I. S. Nam, and J. H. Ahn. 2008. Effects of supplementation of ruminally protected amino acid on in vitro ruminal parameters and milk yield and milk composition of dairy cows in mid-lactation. J. Anim. Sci. Technol. 50:199-208. https://doi.org/10.5187/JAST.2008.50.2.199
  17. Macleod, G. K., Y. Yu, and L. R. Schaeffer. 1977. Feeding value of protected animal tallow for high yielding dairy cows. J. Dairy. Sci. 60:726-738. https://doi.org/10.3168/jds.S0022-0302(77)83927-1
  18. Mattias, J. E., G. J. Ruegsegger, L. H. Schultz, and W. J. Tyler. 1982. Effect of feeding animal fat to dairy cows in early lactation. J. Dairy Sci. 65(Suppl. 1):151.
  19. Ngidi, M. E., S. C. Loerch, F. L. Fluharty, and D. L. Palmquist. 1990. Effects of calcium soaps of long chain fatty acids on feedlot performance, carcass characteristics and ruminal metabolism of steers. J. Anim. Sci. 68:2555-2565. https://doi.org/10.2527/1990.6882555x
  20. Palmquist, D. L. and H. R. Conard. 1978. High fat ration for dairy cows. Effects on feed intake, milk and fat production, and plasma metabolites. J. Dairy Sci. 61:890-901. https://doi.org/10.3168/jds.S0022-0302(78)83667-4
  21. Robinson, P. H., W. Chalapa, W. Julien, K. Sato, H. Suzuki, and R. E. McQueen. 1992. Supplemental rumen-protected amino acids for early lactation dairy cattle. J. Dairy Sci. (Suppl.1):199(abstr).
  22. Roseler, D. K., J. D. Feruson, C. J. Sniffen, and J. Herrema. 1993. Dietary protein degradability effect on plasma and milk urea nitrogen and milk nonprotein nitrogen in Holstein cows. J. Dairy Sci. 76:525-532. https://doi.org/10.3168/jds.S0022-0302(93)77372-5
  23. Sarwar, M., J. L. Firkins, and M. L. Estridge. 1991. Effect of replacing neutral detergent fiber of forage with soy hull and corn gluten feed for dairy heifers. J. Dairy Sci. 74:1006-1017. https://doi.org/10.3168/jds.S0022-0302(91)78250-7
  24. SAS. 1996. User's Guide: Statistics, Version 6.12 Edition. SAS Inst., Inc., Cary, NC, USA.
  25. Schingoethe, D. J., D. P. Casper, C. Yang, D. J. Illg, J. L. Sommerfeldt, and C. R. Mueller. 1988. Lactational response to soybean meal, heated soybean meal, and extrued soybeans with ruminally protected methionine. J. Dairy Sci. 71:173-180. https://doi.org/10.3168/jds.S0022-0302(88)79539-9
  26. Schwab, C. G., C. K. Bozak, N. L. Whitehouse, and V. M. Olsin. 1989. Extent of lysine limitation in mid lactation cows fed a corn-based ration. J. Dairy Sci. 72(Suppl. 1):506(Abstr.).
  27. Sehauff, D. J. and J. H. Clark. 1992. Effect of feeding diets containing calcium salts of long-chain fatty acids to lactating dairy cows. J. Dairy Sci. 75:2990-3002. https://doi.org/10.3168/jds.S0022-0302(92)78063-1
  28. Sharma, H. R., J. R. Inaglls, and J. A. Mckirdy. 1978. Replacing barley with protected tallow in ration of lactating holstein cows. J. Dairy Sci. 61:574-583. https://doi.org/10.3168/jds.S0022-0302(78)94412-0
  29. Sloan, B. K., J. C. Robert, and J. Mathe. 1989. Influence of dietary crude protein of coconut plus or minus inclusion or rumen-protected amino acids (RAA) on the early lactation performance of heifers. J. Dairy Sci. 72(Suppl.1):506(Abstr.).
  30. Sutton, J. D., R. Knight, A. B. McAllan, and R. H. Smith. 1983. Digestion and synthesis in the rumen of sheep given diets supplemented with free or protected oils. Br. J. Nutr. 49:419-432. https://doi.org/10.1079/BJN19830051
  31. Tilly, J. M. A. and R. A. Terry. 1963. A two stage technique for in vitro digestion of forage crop. Grass. Forage Sci. 18:104-111. https://doi.org/10.1111/j.1365-2494.1963.tb00335.x
  32. Veira, D. M., J. R. Seoane, and J. G. Proulx. 1991. Utilization of grass silage by growing cattle: Effect of a supplement contacting ruminally protected amino acids. J. Anim. Sci. 69:4703-4709. https://doi.org/10.2527/1991.69124703x
  33. Wang, A. S., D. E. Jan, K. J. Chen, D. W. Yang, and Y. K. Fan. 2004. Dietary supplementation of fat increased milk percentage without affecting ruminal characteristics in Holstein cows in a warm tropical environment. Asian Australas. J. Anim. Sci. 17:213-220. https://doi.org/10.5713/ajas.2004.213
  34. Wrenn, T. R., J. Bitman, R. A. Waterman, J. R. Weyant, D. L. Wood, L. L. Strozinski, and N. W. Hooven Jr. 1978. Feeding protected and unprotected tallow to lactating cows. J. Dairy Sci. 61:49-58. https://doi.org/10.3168/jds.S0022-0302(78)83550-4
  35. Yang, Y. T., R. L. Baldwin, and J. Russell. 1978. Effect of long supplementation with lipids on lactating dairy cows. J. Dairy Sci. 60:180-188.