• Journal of Life Science
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• v.25 no.10
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• pp.1110-1114
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• 2015

In this study, we investigated the potential of Lactobacillus brevis AR1 fermented broth containing various grains (Fagopyrum esculentum, Scotch oat, Sesamum indicum, Glycine max Merr, Castanea crenata, Oryza sativa L., Hordeum vulgare L., Perilla frutescens var. japonica Hara, or Triticum aestivum L.) or Saccharina japonica as a source of collagen synthesis in cosmetic products. The treatment of Lb. brevis AR1 fermented broths containing F. esculentum or S. japonica water extracts was markedly increased the synthesis of collagen in fibroblasts. The collagen synthesis capacity of the S. japonica fermentation product was higher than that of β-glucan, which was used as a positive control. Under controlled conditions in broths containing F. esculentum or the S. japonica extracts with 4% monosodium glutamate (MSG), Lb. brevis AR1 produced γ-aminobutyric acid (GABA) at a concentration of 180 mM, with an 84.5% GABA conversion rate after 72 h. Both the F. esculentum and S. japonica fermentation broths produced by Lb. brevis AR1 reduced inflammatory responses on mouse skin and did not show cell cytotoxicity in fibroblasts. These results suggest that both the F. esculentum and S. japonica fermentation products of Lb. brevis AR1 could be used as functional materials in cosmetic products to combat wrinkles and skin inflammation.

• Korean journal of applied entomology
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• v.29 no.4
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• pp.269-276
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• 1990

This study investigated host range, seasonal occurrence and developmental characteristics of Ceroplastes pseudoceriferus Green on persimmon trees. The host plants covered 34 families and 66 species, including newly recorded species of Chenopodum album var. centrorubrum, Glycine max, Begonia semperflorens, Pharbitis nil, Perilla frutescens var. japonica and Erigeron canadensis. There was one generation of C. pseudoceriferus a year with larval stage expanding from mid-June to mid-October. Average number of eggs laid per female was 1,073$\pm$177.3. Hatching rate was 97.3% and was not affected by temperatures and photoperiods. Stadium varied as 23.4 days in eggs, 128.3 days in larvae and 213.3 days in female adults including overwintering periods at natural condition.

• Microbiology and Biotechnology Letters
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• v.35 no.3
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• pp.210-219
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• 2007

In this study was investigated the growth effect of native plant (Microlepia marginata(panzer Christ., Prunella vulgaris. aleutica Fernald, Perillafrutescens var. japonica Hara., Gleichenia japonica Spreng) extracts on various lactic acid bacterias, electron donating ability, total cell count and hydroxyl radical scavenging activity. The total cell count of Enterococcus faecium KCCM 12118, Lactobacillus rhamnosus KCCM 32826, Lactobacillus plantarum KCCM 11542, Pediococcus pentosaceus KCCM 40464 in the absence of native plant extracts (10%) at $37^{\circ}C$ after 48hr were $2.2{\times}10^9\;cfu/ml$, $2.1{\times}10^9\;cfu/ml$, $2.3{\times}10^9\;cfu/ml$, $2.2{\times}10^9\;cfu/ml$. On the other hand, the total cell count of E. faecium KCCM 12118, L. rhamnosus KCCM 32826, L. plantarum KCCM 11542, P. pentosaceus KCCM 40464 in the presence of native plant extracts (10%) at $37^{\circ}C$ after 48hr were $4.3{\times}10^9\;cfu/ml$, $4.3{\times}10^9\;cfu/ml$, $4.8{\times}10^9\;cfu/ml$, $3.9{\times}10^9\;cfu/ml$. The electron donating ability indicated to E. faecium KCCM 12118, L. rhamnosus KCCM 32826, L. plantarum KCCM 11542, P. pentosaceus KCCM 40464 added by 10% native plant extracts, respectively. when 10% native plant extracts were added lactic acid bacterias, the electron donating ability is the highest. Hydroxyl radical scavenging activity of E. faecium KCCM 12118, L. rhamnosus KCCM 32826, L. plantarum KCCM 11542, P. pentosaceus KCCM 40464 showed higher than that of control.

• Journal of the Korean Society of Food Culture
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• v.19 no.1
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• pp.94-105
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• 2004

The biochemical components of Namcheondlggae, Miryangdlkkae 25, Boradlggae and Ipdlkkae 1 were measured. The samples were extracted with hot water, 60% acetone or 80% ethanol for screening physiological activity. The crude protein content (4.36%) was found in the Miryangdlkkae 25 and calcium content (497.5 mg%) was found in the Namcheondlggae among the tested 4 perilla loaves. Fructose was 30.86 mg% in the Namcheondlggae and free amino acids at all perilla leaves was detected seventeen. In Boradlggae, glutamic acid and alanine were 25.37 and 11.91 mg%. Totally nine non-volatile organic acids were also detected and the contents of malic acid and glutaric acid were 28.34 and 14.57 mg% in Boradlggae. The Miryangdlkkae 25 had the highest vitamin C amount which was 113.24 mg%. Angiotensin converting enzyme (ACE) inhibition activity of 60% acetone extract of Miryangdlkkae 25 was 39.20% when added as addition of 200 ppm level and xanthine oxidase inhibition activity of 80% ethanol extract of Boradlggae was 46.71%. Electron denoting activity of 60% acetone extract from Namcheohndlggae was the strongest inhibition activity as 98.19% when 200 ppm level of the sample extracts were added.

• Journal of the East Asian Society of Dietary Life
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• v.22 no.2
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• pp.271-277
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• 2012

Ten green leafy vegetables were cultivated from medicinal plant seed. Their nutritional content, taste, appearance and texture were evaluated. Moisture content was $91.23{\pm}0.00%$ in $Saururus$ $chinensis$ (Sc), $90.39{\pm}0.05%$ in $Perilla$ $frutescens$ (Pa) and $66.78{\pm}0.05%$ in $Agastache$ $rugosa$ (Ar). The fat content was 8.89% in $Salvia$ $plebeia$ (Sp) and 0.13% in $Peucedanum$ $japonicum$ (Pj). The protein content was $11.1{\pm}0.00%$ in Ar and $0.54{\pm}0.03%$ in Pj. Total sugar content was $20.06{\pm}0.03%$ in $Geum$ $japonicum$ (Gj), $12.73{\pm}0.03%$ in $Coixlacry$ $majobi$ (Cm) and $1.04{\pm}0.03%$ in $Atractylodes$ $japonica$ (Aj). Medicinal leafy vegetables had higher glucose, sucrose and fructose, but lower maltose. Ascorbic acid content was $33.67{\pm}1.40$ mg% in Sc, $27.91{\pm}2.91$ mg% in Pa and $15.68{\pm}1.09$ mg% in At. Chlorophyll content was higher in $Perilla$ $frutescens$ var. $acuta$ Kudo than other leafy vegetables. The evaluation also showed that Cm, Pa and Aj tasted sweeter and had low acidity.

• Korean Journal of Organic Agriculture
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• v.23 no.4
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• pp.963-974
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• 2015

This study was conducted to determine the effects of organic vegetable cultivation on the soil physical properties in 33 farmlands under plastic greenhouse in Korea. We were investigated 5~8 farms per organic vegetable crops during the period from August to November 2014. The main cultivated vegetables were leafy lettuce (Lactuca sativa L.), Perilla leaves (Perilla frutescens var. Japonica Hara), cucumber (Cucumis sativus L.), strawberry (Fragaria ananassa L.) and tomato (Lycopersicon spp.). We have analyzed soil physical properties. The measured soil physical parameters were soil plough layer, soil hardness, penetration resistance, three soil phase, bulk density and Porosity. The measurement of the soil plough layer, soil hardness and penetration resistance were carried out direct in the fields, and the samples for other parameters were taken using the soil core method with approximately 20 mm diameter core collected from each organic vegetable field. Soil plough layer was average 36 cm and ranged between 30 and 50 cm, and slightly different depending on the sorts of vegetable cultivation. The soil hardness was $0.17{\pm}0.15{\sim}1.34{\pm}1.02$ in the topsoil, $0.55{\pm}0.34{\sim}1.15{\pm}0.62$ in the subsoil. It was not different between topsoil and subsoil, but showed a statistically significant difference between the leafy and fruit vegetables. Penetrometer resistance is one of the important soil physical properties that can determine both root elongation and yield. The increase in density under leafy vegetables resulted in a higher soil penetrometer resistance. Soil is a three-component system comprised of solid, liquid, and gas phases distributed in a complex geometry that creates large solidliquid, liquid-gas, and gas-solid interfacial areas. The three soil phases were dynamic and typically changed in organic vegetable soils under greenhouse. Porosity was characterized as range of $54.2{\pm}2.2{\sim}60.3{\pm}2.4%$. Most measured soils have bulk densities between 1.0 and $1.6gcm^{-3}$. To summarize the above results, Soil plough layer has been deepened in organic vegetable cultivation soils. Solid hardness (the hardness of the soil) and bulk density (suitable for the soil unit mass) have been lowered. Porosity (soil spatial content) was high such as a well known in organic farmlands. Important changes were observed in the physical properties according to the different vegetable cultivation. We have demonstrated that the physical properties of organic cultivated soils under plastic greenhouse were improved in the results of this study.

• Korean Journal of Food Science and Technology
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• v.29 no.4
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• pp.671-679
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• 1997

Approximate composition and mineral content of eighty seven medicinal herbs marketed in Korea was analyzed on the basis of their dried weight. From the results anlyzed, it was known that Acanthopanax sessiliflorum Seem. (22.4%), Portulaca oleracea L. (20.6%), Torilis japonica DC. (15.0%) in crude ash, Ziziyphus spinosa Hu. (39.9%), Alisma orientale Juzep. (30.6%), Lepidium latifolium L. (28.8%), Cassia tora L. (27.5%) in crude protein, Ziziyphus spinosa Hu. (27.9%), Xantnium strumarium L. (24.0%), Perilla frutescens var. acuta Kude (22.8%), Benincasa hispida Cong. (22.4%) in crude lipid, Vitex rotundifolia L. (59.5%), Ganoderma lucidum Karst (53.9%), Pinus densifiora Sieb. et Zucc. (46.4%), Sambucus williamsii Hanse (41.2%) in crude fiber, and Poria cocos Wolf (91.2%), Gastrodiae elata Blume (87.9%), Gentiana scabra Bunge (86.3%), Rehnannia glutinosa Libosch (86.0%) in nitrogen free extract exhibited higher content than other kinds. Phosphrous contents were 1.5, 1.1 and 1.0% in order of Taxillus chinensis Danser, Malva verticillata L., Nelumbo nucifera Gaeten, and magnesium contents were 0.7, 0.5 and 0.5% in the order of Saururus chinesis Baill., Salivia milltriorrhiza Bunge and Pueraria thunberglans Benth. Calcium contents of Cimicifuge heradeifolia Komarov, Acanthopanax sessiliflorum Seem. and Rhus vericiflura Stokes were 8.5, 7.1 and 3.0%, and potassium contents of Tricholoma matsutake Sing., Houttuynia cordata Thunb., Achyranthes japonica Nakai were 5.6, 5,4 and 4.7%, respectively. Sodium contents were 2.1, 0.6 and 0.3% in the order of Boshinakia rossica Fedtsch., Eucommia ulmoides Oliv. and Prunus mume Seib., and iron contents were 0.3, 0.2 and 0.2% in the order of Cirsium japonicum var, ussuriense Kitamuraa, Gentiana scabra Bunge and Phlomis umbrosa Turcz.

• Journal of Food Hygiene and Safety
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• v.37 no.3
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• pp.189-197
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• 2022

Asthma is a chronic inflammatory disease characterized by recurring symptoms, airflow obstruction, and bronchial hyper-responsiveness. The onset of asthma for most patients begins early in life, and current asthma treatment with anti-inflammatory agents can have adverse effects, eventually leading to impaired quality of life. In the pathogenesis of asthma, macrophages and basophils play a vital role during progression. Macrophages not only induce inflammation by secreting inflammatory cytokines but also promote DNA damage and mucus production through nitric oxide (NO) production. Basophils enhance eosinophil recruitment and aggravate asthma through the FcεRIα receptor with high affinity for histamine and IgE. Therefore, in this study, we investigated whether the activation of macrophages and basophils is suppressed by the individual extracts of 28 natural products. RAW 264.7 cells (mouse macrophages) were treated with the natural products in LPS, and 4 natural product extracts resulted in decreased NO production. In β-hexosaminidase assay using RBL-2H3 cells (rat basophils), 19 natural product extracts decreased β-hexosaminidase production. In NO production and β-hexosaminidase assay using macrophages and basophils, 3 natural product extracts (Plantago asiatica, Centella asiatica, and Perilla frutescens var. japonica) significantly inhibited NO production and β-hexosaminidase release. Overall, we examined the inhibitory effects of 28 natural product extracts on macrophage and basophil activity, and the findings demonstrated the potential of natural product extracts for treating asthma and macrophage- and basophil-related diseases.

• Korean journal of food and cookery science
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• v.25 no.5
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• pp.521-530
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• 2009

Perilla leaves powder was added to cookies to determine a practical use for the herb, which has been shown to have medical benefits and functionality. We examined the antioxidant activity and quality characteristics of cookies prepared with different amounts (as ratios of 0.5%, 1%, 3%, 5% to the total materials) of perilla leaves powder. The antioxidant activity was estimated by DPPH free radical scavenging activity and the total phenolic compound content in perilla leaves powder and cookies. The quality characteristics of the perilla leaves cookie were estimated in terms of the bulk density, pH of the dough, spread factor, loss rate, leavening rate, color, texture profile analysis, and sensory evaluations. While the bulk density and pH of the dough as well as total polyphenol contents and DPPH free radical scavenging activity of cookies significantly increased, the spread factor, loss rate, leavening rate and L value of the cookies decreased with increasing perilla leaves powder content(p<.05). The consumer acceptability scores for the $1{\sim}3%$ perilla leaves cookie groups ranked significantly(p<.05) higher than those of the other groups in appearance, taste, flavor, texture, and overall preference. This study suggests that perilla leaves powder is a good ingredient to increase the consumer acceptability and the functionality of cookies.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.65 no.1
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• pp.63-71
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• 2020

The purpose of this study was to analyze the growth characteristics of perilla according to the materials of the seedbed for the development of seedling plug technology suitable for the mechanical transplantation of perilla. Perilla (Perilla frutescens var. japonica Hara) cultivars Deulsaem and Sodam were used in this experiment. The composition ratios of 170 products from 16 companies published in the 'Korean Association of Seedbed Media' homepage were compared according to usage and type, and 11 products that corresponded to the average were selected. The seedbed was classified according to the seedbed for paddy rice as light-weight, semi-weight, and weight, and based on the seedbed for horticulture, as light-weight and ultra-light. The seedlings were placed in 72-cell (semi-automatic), 128-cell (automatic) and 220-cell (automatic) plug trays. We selected 2 light-weight seedbeds of paddy rice and 2 light-weight seedbeds of horticultural products with the highest plant growth. We analyzed plant height and mat formation of the perilla roots. Results showed that the perilla height and mat formation were the best in light-weight seedbeds of paddy rice (product R1). Therefore, light-weight seedbeds of rice (product R1) were suitable for perilla plant transplantation. The estimated major components were vermiculite 41.0%, cocopeat 31.0%, peat moss 5.7%, and red-yellow soil 20.0%. The mechanical transplantation of perilla significantly boosts plant growth and reduces sowing and thinning efforts. However, continuous evaluation of newly introduced, commercial seedbeds is needed.