• Title/Summary/Keyword: Perilla crop

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Analysis of Contamination Sources of Staphylococcus aureus Related to Perilla Leaves Using Multi-Locus Variable Number of Tandem Repeat Analysis (MLVA) (Multi-Locus Variable Number of Tandem Repeat Analysis (MLVA)를 이용한 들깻잎 중 Staphylococcus aureus의 오염원분석)

  • Kim, Se-Ri;Shim, Won-Bo;Han, Noo Ri;Chung, Duck-Hwa
    • Journal of Food Hygiene and Safety
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    • v.29 no.4
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    • pp.278-284
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    • 2014
  • To investigate the prevalence of Staphylococcus aureus (S. aureus) during production of perilla leaves, a total of 261 samples, including water, soil, surroundings of cultivation and packing, workers, and perilla leaves, was examined.. To trace the contamination sources of S. aureus related to perilla leaves, MLVA (Multi-Locous Variable number of tandem repeat Analysis), which is a very efficient method to discriminate strains with minimum molecular biology equipment was applied to S. aureus isolated from perilla leaves farms. S. aureus was isolated in perilla leaves from 9 of 38 farms at 0-2.92 log CFU/g. S. aureus was also found in working environment, including packing vinyl, worker clothes, irrigation water and hands. The patterns of MLVA of isolates from perilla leaves matched with those of isolates from packing table, irrigation water, packing vinyl, and hands. The isolates were successfully examined and determined by MLVA, thus elucidating S. aureus source and spread.

Effects of Stem Extracts from Perilla and Buckwheat on Seed Germination and Seedling Growth of Barnyard grass and Chinese Cabbage (들깨 및 메밀의 건경추출액이 피와 배추의 발아 및 초기생장에 미치는 영향)

  • 성낙술;한의동;우연수
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.32 no.4
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    • pp.443-446
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    • 1987
  • The present study was conducted to investigate the effects of stem extracts from perilla and buckwheat on the germination and seedling growth of barnyard grass and chinese cabbage. The major results obtained are as follows. The stem extracts showed inhibiting effects on seed germination. The degree of inhibition was much higher with the extracts of perilla than those of buckwheat and more sensitive to chinese cabbage than barnyard grass. The trends of inhibition effects on seedling growth were the same as in the seed germination with lower degree. The roots were more sensitive to the extracts than the tops with higher degree with buckwheat extracts.

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The apical bud as a novel explant for high-frequency in vitro plantlet regeneration of Perilla frutescens L. Britton

  • Hossain, H.M.M. Tariq;Kim, Yong-Ho;Lee, Young-Sang
    • Plant Biotechnology Reports
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    • v.4 no.3
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    • pp.229-235
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    • 2010
  • In this study, we established an in vitro regeneration system to maximize the recovery of leafy perilla (Perilla frutescens L. Britton) plantlets as part of developing a molecular biotechnology-based metabolic engineering program for this crop plant. Hypocotyl segments including the apical buds were used as explants for the direct production of shoots without an interim callus phase. The number of shoots produced from the apical buds peaked within 3-4 weeks, and the shoots were subsequently cultured on Murashige and Skoog (MS) media supplemented with 2 mg $1^{-1}$ benzylaminopurine (BA). Spontaneous rhizogenesis was observed after 7-10 days of culture on MS media without hormonal additives. The rooted shoots developed into normal plants in soil after hardening on distilled water for 3-4 days. The average plantlet regeneration frequency was higher for the apical buds (64.33%) than for the top (15.66%), middle (4%), and basal (1.33%) segments of the hypocotyls. This regeneration system demonstrates a capacity for high-frequency plantlet recovery and thus should be considered for use in the genetic manipulation of leafy perilla.