Plant Biotechnology Reports

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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The apical bud as a novel explant for high-frequency in vitro plantlet regeneration of Perilla frutescens L. Britton

  • Received : 2009.06.07
  • Accepted : 2010.06.09
  • Published : 2010.09.30
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Abstract

In this study, we established an in vitro regeneration system to maximize the recovery of leafy perilla (Perilla frutescens L. Britton) plantlets as part of developing a molecular biotechnology-based metabolic engineering program for this crop plant. Hypocotyl segments including the apical buds were used as explants for the direct production of shoots without an interim callus phase. The number of shoots produced from the apical buds peaked within 3-4 weeks, and the shoots were subsequently cultured on Murashige and Skoog (MS) media supplemented with 2 mg $1^{-1}$ benzylaminopurine (BA). Spontaneous rhizogenesis was observed after 7-10 days of culture on MS media without hormonal additives. The rooted shoots developed into normal plants in soil after hardening on distilled water for 3-4 days. The average plantlet regeneration frequency was higher for the apical buds (64.33%) than for the top (15.66%), middle (4%), and basal (1.33%) segments of the hypocotyls. This regeneration system demonstrates a capacity for high-frequency plantlet recovery and thus should be considered for use in the genetic manipulation of leafy perilla.

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References

  1. Ahloowalia BS (1991) Somatic embryos in monocots: their genesis and genetic stability. Rev Cytol Biol Veg Bot 14:223-235
  2. Chang C, Moll BA, Evenson KB, Guiltinan MJ (1996) In vitro plantlet regeneration from cotyledon, hypocotyls and root explants of hybrid seed geranium. Plant Cell Tissue Organ Cult 45:61-66 https://doi.org/10.1007/BF00043429
  3. Choi IS, Son SY, Kwon OH (1980) Effect of seedling age and planting density on the yield and its components of perilla (Ocymcides var Typica MAKINO) intercropped with tobacco or after-cropped. J Korean Soc Hortic Sci 25:68-75
  4. Choi PS, Soh WY, Liu JR (1996) Somatic embryogenesis and plant regeneration in cotyledonary explant cultures of Chinese cabbage. Plant Cell Tissue Organ Cult 44:253-256 https://doi.org/10.1007/BF00048532
  5. Deklerk GJ, Hanecakova J, Jasik J (2001) The role of cytokinins in rooting of stem slices cut from apple microcuttings. Plant Biosyst 135:79-84 https://doi.org/10.1080/11263500112331350680
  6. Dimech AM, Cross R, Ford R, Taylor PWJ (2007) Micropropagation of Gymea lily (Doryanthes excelsa Correa) from New South Wales, Australia. Plant Cell Tissue Organ Cult 88:157-165 https://doi.org/10.1007/s11240-006-9184-1
  7. Gaspar T, Coumans M (1987) Root formation. In: Bong JM, Durzan DJ (eds) Cell and tissue culture in forestry. Martinus Nijhoff, Dordrecht, pp 202-217
  8. Haliloglu K (2006) Efficient regeneration system from wheat leaf base segments. Biol Plant 50:326-330 https://doi.org/10.1007/s10535-006-0045-x
  9. Hansena G, Wright MS (1999) Recent advances in the transformation of plants. Trends Plant Sci 4:226-231 https://doi.org/10.1016/S1360-1385(99)01412-0
  10. Honda G, Koezuka Y, Tabata M (1990) Genetic studies of color and hardness in Perilla frutescens (L). Jpn J Breed 40:469-474 https://doi.org/10.1270/jsbbs1951.40.469
  11. Hou SW, Jia JF (2005) In vitro regeneration of Perilla frutescens from hypocotyls and cotyledon explants. Biol Plant 49:129-132 https://doi.org/10.1007/s10535-005-0132-4
  12. Kim KM, Lee WS (2007) High frequency plant regeneration from the cultures of cotyledon explants of perilla (Perilla frutescens L.). J Plant Biotechnol 34:69-73 https://doi.org/10.5010/JPB.2007.34.1.069
  13. Kim JA, Choi HJ, Park SK, Kim DV (1993) Callus induction and plant regeneration from leaf segments and cotyledonary explants of Perilla frutescens (L.). Korean J Plant Tissue Cult 20:47-50
  14. Kim KH, Lee YH, Kim D, Park YH, Lee JY, Hwang YS, Kim YH (2004) Agrobacterium mediated genetic transformation of Perilla frutescens. Plant Cell Rep 23:386-390 https://doi.org/10.1007/s00299-004-0825-8
  15. Koezuka Y, Honda G, Sakamoto S, Tabata M (1985) Genetic control of anthocyanin production in Perilla frutescens. Shoyakuga Ku Zasshi 39:228-231
  16. Kurita N, Koike S (1981) Synergistic antimicrobial effect of perilla and NaCl. Nippon Nogeikagaku Kaichi 55:43-46 https://doi.org/10.1271/nogeikagaku1924.55.43
  17. Lee HS, Lee JI, Ryu SN, Hur HS (1994) Effect of low temperature and plant growth regulators on callus induction and plant regeneration in anther culture of perilla. Korean J Breed 26:345-352
  18. Lee JK, Nitta M, Kim NS, Park CH, Yoon KM, Shin YB, Ohnishi O (2002) Genetic diversity of perilla and related weedy types in Korea determined by AFLP analysis. Crop Sci 42:2161-2166 https://doi.org/10.2135/cropsci2002.2161
  19. Lee BK, Yu SH, Kim YH, Hur HS, Lu SC, Lee JY (2003) Efficient in vitro shoot regeneration of perilla (Perilla frutescens) from cotyledon, hypocotyls and leaf explants. Korean J Breed 35:237-240
  20. Li HL (1969) The vegetables of ancient China. Econ Bot 23:253-260 https://doi.org/10.1007/BF02860457
  21. Lin HS, Jeu MJD, Jacobson E (1998) Formation of shoots from leaf axils of Alstroemeria: the effect of the position on the stem. Plant Cell Tissue Organ CultPlant Cell Tissue Organ Cult 52:165-169 https://doi.org/10.1023/A:1006063105940
  22. Makino T, Ono T, Muso E (2002) Effect of Perilla frutescens on nitric oxide production and DNA synthesis in cultured murine vascular smooth muscle cells. Phytother Res 16:19-23 https://doi.org/10.1002/ptr.781
  23. Mala J, Gaudinova A, Dobrev P, Eder J, Cvikrova M (2005) Role of phytohormones in organogeneic ability of elm multiplicated shoots. Biol Plant 50:8-14
  24. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  25. Nitta M (2001) Origin of perilla crops and their weedy type (Ph.D. dissertation). Kyoto University, Kyoto
  26. Park CB, Bang JK, Lee BK, Kim JK, Lee BH (2000) Research activity and achievement in mutation breeding of perilla in Korea. Korean J Int Agric 12:108-115
  27. Rey HY, Mroginski LA (2006) Somatic embryogenesis and plant regeneration in diploid and triploid Arachis pintoi. Biol Plant 50:152-155 https://doi.org/10.1007/s10535-005-0093-7
  28. Sawabe A, Satake T, Aizawa R, Sakatani K, Nishimoto K, Ozeki C, Hamada Y, Komemushi S (2006) Toward use of the leaves of Perilla frutescens (L.) Britton var. Acuta Kudo (red perilla) with Japanese dietary pickled plum (Umeboshi). J Oleo Sci 55:413-422 https://doi.org/10.5650/jos.55.413
  29. Sonia, Saini R, Singh RP, Jaiwal PK (2007) Agrobacterium tumefaciens mediated transfer of Phaseolus vulgaris ${\alpha}-amylase$ inhibitor-1 into mungbean Vigna radiata (L.) Wilczek using bar as selectable marker. Plant Cell Rep 26:187-198 https://doi.org/10.1007/s00299-006-0224-4
  30. Takeda H, Tsuzi M, Matsumiya T (2002) Identification of rosmarinic acid as a novel antidepressive substance in the leaves of Perilla frutescens Britton var acuta Kudo. Jpn. J Neuropsychopharmacol 22:15-22
  31. Torres KC (1989) Tissue culture techniques for horticultural crops. AVI-Van Nostrand, New York
  32. Vasil IK (1987) Developing cell and tissue culture systems for the improvement of cereal and grass crops. J Plant Physiol 128:193-218 https://doi.org/10.1016/S0176-1617(87)80234-1
  33. Vazquez Molina DE, De Los Santos A, Lecona Guzman KA, Sumano Muniz O, Velazquez Mendez M, Rincon Rosales R, Oliva Llaven MA, Dendooven L, Guiterrez-Miceli FA (2005) Sugar cane buds as an efficient explant for plantlet regeneration. Biol Plant 49:481-485 https://doi.org/10.1007/s10535-005-0035-4
  34. Zhang T, Wang XY, Cao ZY (2005) Plant regeneration in vitro directly from cotyledon and hypocotyls explants of Perilla frutescens and their morphological aspects. Biol Plant 49:423-426 https://doi.org/10.1007/s10535-005-0020-y

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