• Journal of Communications and Networks
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• v.13 no.3
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• pp.232-239
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• 2011

This paper investigates the user selection problem of successive zero-forcing precoded multiuser multiple-input multiple-output (MU-MIMO) downlink systems, in which the base station and mobile receivers are equipped with multiple antennas. Assuming full knowledge of the channel state information at the transmitter, dirty paper coding (DPC) is an optimal precoding strategy, but practical implementation is difficult because of its excessive complexity. As a suboptimal DPC solution, successive zero-forcing DPC (SZF-DPC) was recently proposed; it employs partial interference cancellation at the transmitter with dirty paper encoding. Because of a dimensionality constraint, the base station may select a subset of users to serve in order to maximize the total throughput. The exhaustive search algorithm is optimal; however, its computational complexity is prohibitive. In this paper, we develop two low-complexity user scheduling algorithms to maximize the sum rate capacity of MU-MIMO systems with SZF-DPC. Both algorithms add one user at a time. The first algorithm selects the user with the maximum product of the maximum column norm and maximum eigenvalue. The second algorithm selects the user with the maximum product of the minimum column norm and minimum eigenvalue. Simulation results demonstrate that the second algorithm achieves a performance similar to that of a previously proposed capacity-based selection algorithm at a high signal-to-noise (SNR), and the first algorithm achieves performance very similar to that of a capacity-based algorithm at a low SNR, but both do so with much lower complexity.

• Journal of Microbiology and Biotechnology
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• v.18 no.12
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• pp.1938-1944
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• 2008

The procpb gene encoding human procarboxypeptidase B (proCPB, GeneBank access code AJ224866) was cloned and its Pichia expression plasmid, $pPIC9{\alpha}$/hproCPB (9.2 kb), was constructed, in which procpb was under the control of the AOXl promoter and connected to the downstream of the mating factor ${\alpha}$-1 ($MF{\alpha}1$) signal sequence. The plasmid was linearized by digestion with Sacl, and integrated into the genome of P. pastoris strain GS115. By culturing of Pichia transformant on methanol medium, the human proCPB was successfully expressed and secreted into the culture supernatant. Moreover, Western blot analysis of the extracellular proteins showed proCPB bands clearly at a molecular mass of 45 kDa, confirming the expression of proCPB with its right size. The CPB activity reached about 3.5 U/ml and 12.7 U/ml in the flask and fermentor batch cultures of Pichia transformant, respectively. No CPB enzyme activity was found in the intracellular fraction. When the fed-batch cultivation was performed with methanol and glycerol mixture as a feeding medium, the extracellular CPB activity was increased to 42.0 U/ml, which corresponds to a 3.3-fold higher level of CPB activity than that of batch culture. The $K_m$ and $k_{cat}$ values of recombinant human CPB enzyme for hippuryl-$_L$-Arg as a substrate were estimated to be 0.16 mM and $11.93\;sec^{-1}$, respectively.

• Archives of Plastic Surgery
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• v.36 no.1
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• pp.1-10
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• 2009

Purpose: Partial necrosis of skin flaps remains a substantial problem in reconstructive surgery. We investigated the potential use of an adenovirus vector encoding the VEGF, COMP-angiopoietin-1 gene in an attempt to promote the viability of the inferior epigastric artery flap in a rat model. Methods: Three by six cm lower abdominal transverse skin flaps, supplied only by the left inferior epigastric artery, were designed. After skin flap elevation, the adenovirus VEGF and adenovirus COMP-angiopoietin-1 were injected into the distal portion of the flap, which has a high tendency of developing flap ischemia. Control animals were injected with the same volume of normal saline. On 3, 7 and 14 days after the flap elevation, the flap survival and vascularization were assessed using Visitrak digital$^{(R)}$, CD31 immunohistochemistry in addition to evaluating the general histological characteristics. Results: There was a significant increase in the mean percentage of flap viability by 89.8%, 91.1% and 94.8% in flaps transfected with adenovirus VEGF, COMP-angiopoietin-1, coadministraion of VEGF and COMP-angiopoietin-1 at seven days, and by 95.6%, 94.8% and 96.3% at 14 days. Histological assessment revealed that there were more blood vessels formed after adenovirus with VEGF, COMP-angiopoietin-1 or VEGF plus COMP-angiopoietin-1 than with adenovirus Lac Z. Conclusion: The results of this study suggest that adenovirus-mediated VEGF, COMP-angiopoietin-1 gene therapy, promote therapeutic angiogenesis in patients that undergo reconstructive procedures.

• Applied Biological Chemistry
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• v.31 no.3
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• pp.274-283
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• 1988

Aqualysin I is an alkaline serine protease which is secretet into the culture medium by Thermus aquaticus YT-1, an extreme thermophile. Aqualysin I was purified, and its partial amino acid sequence was determined. The gene encoding aqualysin I was cloned into E. coli using synthetic oligodeoxyribonucleotides as hybridization probes. The nucleotide sequence of the cloned DNA was determined. The primary structure of aqualysin I, deduced from the nucleotide sequenc, agreed with the determid amino acid sequences, including the $NH_2-$ and COOH terminal sequence of the tryptides derived from aqualysin I. Aqualysin I comprised 281 amino acid residues and its molecular mass was determined to be 28350. On alignment of the whole amino acid sequence, aqualysin I showed high sequence homology with the subtilisin type serine protease, and 43% identity with proteinase K, 37-30% with subtilisins and 34% with thermitase. Extremely high sequence identity was observed in the regions containing the active-site residues, corresponding to Asp32, His64 and Ser221 of subtilisin BPN'. Aqualysin I contains two disulfide bonds, Cys67-Cys99 and Cys163-Cys194, and these disulfide bonds seem to contribute to the heat stability of the enzyme. The determined positions of the twe disulfide bonds of aqualysin I agreed with those predicted previously on the basis of computer graphics of the crystallographic data for subtilisin BPN'. Therefore, these findings sugests that the three-dimensional structure of aqualysin I is similar to that of subtilisin BPN' Aqualysin I is produced as a lage precursor, which contains $NH_2-$ and COOH- terminal portions besides the mature protease sequence.

• Parasites, Hosts and Diseases
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• v.52 no.3
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• pp.251-256
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• 2014

A novel recombinant Bacille Calmette-Guerin (rBCG) vaccine co-expressed Eimeria tenella rhomboid and cytokine chicken IL-2 (chIL-2) was constructed, and its efficacy against E. tenella challenge was observed. The rhomboid gene of E. tenella and chIL-2 gene were subcloned into integrative expression vector pMV361, producing vaccines rBCG pMV361-rho and pMV361-rho-IL2. Animal experiment via intranasal and subcutaneous route in chickens was carried out to evaluate the immune efficacy of the vaccines. The results indicated that these rBCG vaccines could obviously alleviate cacal lesions and oocyst output. Intranasal immunization with pMV361-rho and pMV361-rho-IL2 elicited better protective immunity against E. tenella than subcutaneous immunization. Splenocytes from chickens immunized with either rBCG pMV361-rho and pMV361-rho-IL2 had increased $CD4^+$ and $CD8^+$ cell production. Our data indicate recombinant BCG is able to impart partial protection against E. tenella challenge and co-expression of cytokine with antigen was an effective strategy to improve vaccine immunity.

• Journal of Korea Spatial Information System Society
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• v.8 no.1 s.16
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• pp.77-92
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• 2006

This paper proposes an efficient spatial data manager for map services in mobile environment. It is designed to provide interoperability and efficient performance at once. To provide interoperability and reusability, the spatial data manager conforms to international standards such as the OpenGIS Simple Features Implementation Specification for OLE/COM, OpenGIS Geography Markup Language (GML) Encoding Specification developed by the Open Geospatial Consortium (OGC). The spatial data manger exploits physical main memory using Address Windowing Extensions supported by Microsoft Windows to manage spatial data for efficient performance by reducing time to read data from disk on user's request. The format of the spatial data in main memory is target data (GML) to reduce conversion time from source data to it. Progressive transmission is also provided to reduce latency time by representing only received partial data for mobile environment without waiting whole transmission.

• Journal of Aquaculture
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• v.22 no.1
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• pp.79-82
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• 2009

Reciprocal interspecific hybrids between two bitterling species Rhodeus uyekii (RU) and R. notatus (RN) were genetically identified based on the partial sequence analysis of recombination activating gene-1 (RAG-1) gene. Out of 863 bp positions analyzed, 13 nucleotide substitutions were detected between the two parental species (RU and RN genotypes). Both the induced hybrids (RU female$\times$RN male; UN genotype) and their reciprocal counterparts (RN female$\times$RU male; NU genotype) displayed the double peaks (or polymorphism) of sequence chromatograms at the 13 diagnostic positions, indicating that those hybrids were actual karyogamy derived from the two parental haploid genomes. However, it was not possible to distinguish between the reciprocal interspecific hybrids.

• The Korean Journal of Mycology
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• v.37 no.2
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• pp.155-160
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• 2009

The production of dihydroxynaphthalene (DHN) melanin is known to be essential factor for pathogenicity in Colletotrichum lagenarium. However, the genetic diversity of melanin genes was not much known among Colletotrichum spp. To investigate the variability of melanin gene in Colletotrichum spp. that cause anthracnose on diverse crops including tomato, we cloned and sequenced partial sd, one of DHN melanin genes encoding for scytalone dehydratase, from eight strains of C. coccodes, C. acutatum, C. truncatum C. caricae, and C. musae. The size of PCR-amplified sd ranged 437 bp to 545 bp. The nucleotide sequence identity of sd among the Colletotrichum strains tested varied from 49% to 99%. All of the PCR-amplified sd from eight strains contain an intron and have two exons coding for 122 amino acids. Overall, the size and nucleotide sequence of sd varied among the five Colletotrichum spp. Sequence identity of the predicted scytalone dehydratase protein of 122 amino acids ranged 50 to 99%. Phylogentic analysis based on the sd nucleotide sequences revealed that the five Colletotrichum spp. could be genetically divided.

• Journal of the Institute of Electronics and Information Engineers
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• v.52 no.4
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• pp.115-124
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• 2015

In this paper, the method which improves the performance of a serial decimal multiplier, and the method which operates multiple-digit simultaneously are proposed. The proposed serial decimal multiplier reduces the delay by removing encoding module that generates 2X, 4X multiples, and by generating partial product using shift operation. Also, this multiplier reduces the number of operations using multiple-digit operation. In order to estimate the performance of the proposed multiplier, we synthesized the proposed multiplier with design compiler with SMIC 110nm CMOS library. Synthesis results show that the area of the proposed serial decimal multiplier is increased by 4%, but the delay is reduced by 5% compared to existing serial decimal multiplier. In addition, the trade off between area and latency with respect to the number of concurrent operations in the proposed multiple-digit multiplier is confirmed.

• Journal of the Institute of Convergence Signal Processing
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• v.21 no.2
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• pp.55-60
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• 2020

In this paper, we propose a fast block matching algorithm of motion estimation using early detection of optimal candidate with high priority and a threshold. Even though so many fast algorithms for motion estimation have been published to reduce computational reduction full search algorithm, still so many works to improve performance of motion estimation are being reported. The proposed algorithm calculates block matching error for each candidate with high priority from previous partial matching error. The proposed algorithm can be applied additionally to most of conventional fast block matching algorithms for more speed up. By doing that, we can find the minimum error point early and get speed up by reducing unnecessary computations of impossible candidates. The proposed algorithm uses smaller computation than conventional fast full search algorithms with the same prediction quality as the full search algorithm. Experimental results shows that the proposed algorithm reduces 30~70% compared with the computation of the PDE and full search algorithms without any degradation of prediction quality and further reduces it with other fast lossy algorithms.