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Genetic Identification of Hybrids between Rhodeus uyekii and R. notatus by Sequence Analysis of RAG-1 Gene  

Yun, Young-Eun (Department of Marine Biotechnology, Soonchunhyang University)
Lee, Il-Ro (Department of Marine Biotechnology, Soonchunhyang University)
Park, Sang-Yong (Department of Marine Biotechnology, Soonchunhyang University)
Kang, Eon-Jong (Southern Regional Inland Research Institute, NFRDI)
Kim, Eung-O (Southern Regional Inland Research Institute, NFRDI)
Yang, Sang-Keun (Southern Regional Inland Research Institute, NFRDI)
Nam, Yoon-Kwon (Department of Aquaculture, Pukyong National University)
Bang, In-Chul (Department of Marine Biotechnology, Soonchunhyang University)
Publication Information
Journal of Aquaculture / v.22, no.1, 2009 , pp. 79-82 More about this Journal
Abstract
Reciprocal interspecific hybrids between two bitterling species Rhodeus uyekii (RU) and R. notatus (RN) were genetically identified based on the partial sequence analysis of recombination activating gene-1 (RAG-1) gene. Out of 863 bp positions analyzed, 13 nucleotide substitutions were detected between the two parental species (RU and RN genotypes). Both the induced hybrids (RU female$\times$RN male; UN genotype) and their reciprocal counterparts (RN female$\times$RU male; NU genotype) displayed the double peaks (or polymorphism) of sequence chromatograms at the 13 diagnostic positions, indicating that those hybrids were actual karyogamy derived from the two parental haploid genomes. However, it was not possible to distinguish between the reciprocal interspecific hybrids.
Keywords
Hybrids; Bitterlings; Rhodeus uyekii; R. notatus; RAG-1; Sequence polymorphism;
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