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http://dx.doi.org/10.4489/KJM.2009.37.2.155

Genetic Variation of Scytalone Dehydratase Gene in Colletotrichum spp.  

Yun, Yeo-Hong (Department of Microbiology, Dankook University)
Hyeon, Min-U (Department of Microbiology, Dankook University)
Suh, Dong-Yeon (Department of Microbiology, Dankook University)
Kim, Yong-Min (Department of Microbiology, Dankook University)
Kim, Seong-Hwan (Department of Microbiology, Dankook University,Institute of Basic Sciences, Dankook University)
Choi, Chang-Won (Department of Biology and Medicinal Science, Paichai University)
Kim, Young-Shick (Department of Plant Science and Technology, Sangmyung University)
Publication Information
The Korean Journal of Mycology / v.37, no.2, 2009 , pp. 155-160 More about this Journal
Abstract
The production of dihydroxynaphthalene (DHN) melanin is known to be essential factor for pathogenicity in Colletotrichum lagenarium. However, the genetic diversity of melanin genes was not much known among Colletotrichum spp. To investigate the variability of melanin gene in Colletotrichum spp. that cause anthracnose on diverse crops including tomato, we cloned and sequenced partial sd, one of DHN melanin genes encoding for scytalone dehydratase, from eight strains of C. coccodes, C. acutatum, C. truncatum C. caricae, and C. musae. The size of PCR-amplified sd ranged 437 bp to 545 bp. The nucleotide sequence identity of sd among the Colletotrichum strains tested varied from 49% to 99%. All of the PCR-amplified sd from eight strains contain an intron and have two exons coding for 122 amino acids. Overall, the size and nucleotide sequence of sd varied among the five Colletotrichum spp. Sequence identity of the predicted scytalone dehydratase protein of 122 amino acids ranged 50 to 99%. Phylogentic analysis based on the sd nucleotide sequences revealed that the five Colletotrichum spp. could be genetically divided.
Keywords
Anthracnose; Colletotrichum spp.; DHN melanin; Scytalone dehydratase gene; Tomato;
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1 Perpetua, N. S., Kubo, Y., Yasuda, N., Takano, Y. and Furusawa, I. 1996. Cloning and characterization of a melanin biosynthetic THR1 reductase gene essential for appressorial penetration of Colletotrichum lagenarium. Mol. Plant-Microbe Interact. 9:323-329.   DOI   ScienceOn
2 Butler, M. J. and Day, A. W. 1998. Fungal melanins: a review. Can. J. Microbiol. 44:1115-1136.   DOI   ScienceOn
3 Howard, R. J. and Ferrai, M. A. 1989. Role of melanin in appressorium function. Exp. Mycol. 13:403-418.   DOI
4 Kim, W. G., Moon, Y. G., Cho, W. D. and Park, S. D. 1999. Anthracnose of safflower caused by Colletotrichum acutatum. Plant Pathol. J. 15:62-67.
5 Kimura, M. 1980. A simple method for estimating evolutionary rate of base substitution through comparative studies of nucleotide sequence. J. Mol. Evol. 16:111-120.   DOI
6 Kubo, Y., Tsuda, M., Furusawa, I. and Shishiyama, J. 1989. Genetic analysis of genes involved in melanin biosynthesis of Cochliobolus miyabeanus. Exp. Mycol. 13:77-84.   DOI
7 Kubo, Y., Takano, Y., Endo, N., Yasuda, N., Tajima, S. and Furusawa, I. 1996. Cloning and structural analysis of the melanin biosynthesis gene SCD1 encoding scytalone dehydratase in Colletotrichum lagenarium. Appl. Environ. Microbiol. 62:4340-4344.
8 Swofford, D. L. 2002. PAUP: Phylogenetic analysis using parsimony (*and other methids). Version 4.0 b10. Sinauer associates, Sunderland, MA.
9 Wang, H. L., Kim S. H. and Breuil, C. 2001. A scytalone dehydratase gene from Ophiostoma floccosum restores the melanization and pathogenicity phenotypes of a melanindeficient Colletotrichum lagenarium mutant. Mol. Genet. Genomics. 266:126-132.   DOI   ScienceOn
10 Zeun, R., and Buchenauer, H. 1985. Effect of tricyclazole on production and melanin contents of sclerotia of Botrytis cinerea. Phytopathol. Z. 112:259-267.   DOI
11 Woloshuk, C. P., Sisler, H. D., Tokousbalides, C. M. and Dutky, S. R. 1980. Melanin biosynthesis in Pyricularia oryzae: Site of tricyclazole inhibition and pathogenicity of melanin-deficient mutants. Pestic. Biochem. Physiol. 14:256-264.   DOI
12 Kim, S. H., Uzunovic, A. and Breuil, C. 1999. Rapid detection of Ophiostoma piceae and O. quercus in stained wood by PCR. Appl. Environ. Microbiol. 65:287-290.
13 Bell, A. A. and Wheeler, M. H. 1986. Biosynthesis and functions of fungal melanins. Annu. Rev. Phytopathol. 24:411-451.   DOI   ScienceOn
14 Kubo, Y., Katoh, M., Furusawa, I. and Shishiyama, J. 1986. Inhibition of melanin biosynthesis by cerulenin in appressoria of Colletotrichum lagenarium. Exp. Mycol. 10:301-306.   DOI