• Analytical Science and Technology
• /
• v.26 no.3
• /
• pp.182-189
• /
• 2013

The purpose of this research is to separate and quantitate selenium species in some food samples with HPLC-ICP-MS. Cation exchange chromatography showed efficient separation only for inorganic Se species while reversed phase ion pair chromatography showed good separation for both inorganic and organic Se species. $C_8$ column ($Symmetryshield^{TM}\;RP_8$, 3.5 ${\mu}m$, $4.6{\times}150$ mm) was used with optimum condition of 5% methanol mobile phase, 0.05% of nonafluorovaleric acid ion pairing reagent. Five standard Se species of Se(IV), Se(VI), SeCys(selenocystein), SeMet(selenomethionine) and Se-M-C(seleno methyl cystein) were separated successfully under the optimum condition (mobile phase; 5% methanol, ion-pairing reagent; 0.05% nonafluorovaleric acid, flow rate; 0.9 mL $min^{-1}$). To extract Se species, microwave assisted and enzyme-assisted extraction methods were studied. In enzyme-assisted extraction method, protease I for garlic, protease I plus trypsin for pork and mackerel, and protease XIV for tuna showed the best extraction efficiency. With the optimum condition for each sample, it was found that mostly inorganic Se, SeCys and SeMet are present in the sample studied ranging from few ${\mu}g$ $g^{-1}$ to few tens of ${\mu}g$ $g^{-1}$.

• Journal of the Korea Institute of Military Science and Technology
• /
• v.12 no.6
• /
• pp.733-738
• /
• 2009

In this paper, we have proposed the design and implementation for efficient Command Control and Alert(C2A). Information fusion must be done for knowing the state and identification of targets using multi-sensor. The threat priority of targets which are processed and identified by information fusion is calculated by air-defence operation logic. The threat targets are assigned to the valid and effective weapons by nearest neighborhood algorithm. Furthermore, the assignment result allows operators to effectively operate C2A by providing the operators with visualizing symbol color and the assignment pairing color line. We introduce the prototype which is implemented by the proposed design and algorithm.

• Journal of the Korean Magnetic Resonance Society
• /
• v.19 no.3
• /
• pp.143-148
• /
• 2015

In some pathogenic bacteria, there are RNA thermometers, which regulate the production of virulence associated factors or heat shock proteins depending on temperature changes. Like a riboswitches, RNA thermometers are located in the 5'-untranslated region and involved translational gene regulatory mechanism. RNA thermometers block the ribosome-binding site and start codon area under the $37^{\circ}C$ within their secondary structure. After bacterial infection, increased the temperature in the host causes conformations changes of RNA, and the ribosome-binding site is exposed for translational initiation. Because structural differences between open and closed forms of RNA thermometers are mainly mediated by base pairing changes, NMR spectroscopy is a very useful method to study these thermodynamically changing RNA structure. In this review, we briefly provide a fundamental function of RNA thermometers, and also suggest a proper NMR experiments for studying RNA thermometers.

• Journal of Advanced Navigation Technology
• /
• v.15 no.2
• /
• pp.221-226
• /
• 2011

This paper presents a DTMB deinterleaver structure to reduce SDRAM power consumption. DTMB, the Chinese digital TV standard, has a deinterleaver that consists of many long delay buffers. SDRAM is used for this deinterleaver. The proposed structure pairs data and transfer a pair with an SDRAM transfer. With the reduction of the SDRAM operation number, the proposed structure can save the SDRAM power consumption by around 35%.

• 한국정보디스플레이학회:학술대회논문집
• /
• 2009.10a
• /
• pp.1469-1471
• /
• 2009

Effect of contamination of liquid crystal on the electrical behavior of liquid crystal display (LCD) by spilled components from color filter layer was investigated. It is noted that the level of contamination was different following the kind of liquid crystal and organic layers. Transmittance curve of LCD cell was shifted by spilled components of color filter and BM materials. C-V curve also showed different behavior following color filter type. The results obtained show the image sticking of LCD can be varied by pairing liquid crystal with color filter materials.

• Journal of Communications and Networks
• /
• v.14 no.2
• /
• pp.199-205
• /
• 2012

This work investigates the progressive linear precoder design for packet retransmissions in multi-input multi-output (MIMO) systems with multiple codewords and automatic repeat request (ARQ) bundling feedback. Assuming perfect channel state information, a novel progressive linear ARQ precoder is proposed in the perspective of minimizing the packet error rate. We devise the ARQ precoder by combining power loading and sub channel pairing between current retransmission and previous transmissions. Furthermore, we extend the design to the case that the channel estimation error exists. Numerical results show that the proposed scheme can improve the performance of MIMO ARQ systems significantly regardless of the channel estimation error.

• Journal of the Korea Institute of Military Science and Technology
• /
• v.2 no.2
• /
• pp.279-286
• /
• 1999

Wall-bounded 유동과 달리 자유 전단 유동은 Hyperbolic Tangent Profile을 가지고 비점성 불안정에 의해 지배된다. 따라서 자유 전단 유동에서 난류로의 천이과정은 비점성 불안정성 이론에 의해 해석되어 진다. 본 연구는 분리판(Splitter plate)에 의해 분리된 속도가 다른 자유 유속의 혼합에 의해 형성되는 혼합층에서 와류병합과정에 대한 속도비의 영향에 대하여 연구한다. 속도비는, R, $ \frac{U_1-U_2}{U_1+U_2}$ 로 정의되며, 여기서 $U_1$은 분리판 위에서의 자유 유속을 그리고 $U_2$ 는 분리판 아래에서의 자유 유속을 나타낸다. 본 연구에서 와류구조의 병합작용을 분석하기 위하여 2차원 비정상 Large-Eddy Simulation 방법을 적용하였다. 속도비의 변화에 따라 혼합층에서 불안정 Wave가 성장하게 되고, 유체는 2차원 와류구조에서 Roll-up한다. 이러한 2차원 와류구조는 주위의 다른 와류구조와 상호작용을 하게 되고 하나의 커다란 와류구조를 형성하는 것을 볼 수 있다. 혼합층에서 와류병합과정은 반복적으로 일어나는 것을 알 수 있었고, 이 결과를 이용하여 혼합층의 성장을 제어할 수 있다.

• Archives of Pharmacal Research
• /
• v.20 no.5
• /
• pp.438-442
• /
• 1997

Antisense oligonucleotide represents an interesting tool for selective inhibition of gene expression. However, their low efficiency of introduction within intact cells remains to be overcome. Antisense-$TGF{\beta}$ (25 mer) and antisense-$TGF{\beta}$ (18 mer) were used to study the cellular transport and biological function of antisense oligonucleotide in vitro. Since TGF and TNF play on important role in regulating the nitric oxide production from macrophages, the action of the above antisense oligonucleotides was easily monitored by the determination of nitrite. Poly-L-lysine, benzalkonium chloride and tetraphenylphosphonium chloride were used as polycations, which neutralize the negative charge of antisense oligonucleotide. The production of nitric oxide mediated by .gamma.-IFN in mouse peritoneal macrophage was increased by antisense-TGF.betha. in a dose-dependent manner. Antisense-$TGF{\beta}$ reduced the nitric oxide release from activated RAW 264.7 cells. Significant enhancement in the nitric oxide production was investigated by the cotreatment of poly-L-lysine with antisense-$TGF{\beta}$On the meanwhile, inhibition effect of antisense-$TGF{\beta}$ is not changed by the addition of poly-L-lysine. These results demonstrate that control of expression of $TGF{\beta}$ and TNF.alpha. gene is achieved using antisense technology and the cellular uptake of antisense oligonucleotide could be enhanced by ion-pairing.

• Analytical Science and Technology
• /
• v.9 no.3
• /
• pp.292-301
• /
• 1996

The synthesis of poly(ether)tailed bipyridine complex as redox reaction probes has advanced attempts to interpret very slow diffusion and heterogeneous electron transfer. Diffusion coefficients as low as $1.5{\times}10^{-15}cm^2/s$ have been observed for the oxidation of neat $Co(bpy(ppgm)_2)_{3^-}(ClO_4)_2$ with $LiClO_4$ electrolyte. Heterogeneous electron transfer rate constants of materials were found to vary with diffusion coefficient. The decrease in k as the diffusion coefficient decreases was actually caused by the decreasing D. Diffusion coefficient for compound of strong ion pairing anion($ClO{_4}^-$) was much smaller than the diffusion coefficient for compound of weak ion pairing anion($CF_3COO^-$).

• Analytical Science and Technology
• /
• v.12 no.1
• /
• pp.28-33
• /
• 1999

Glutathione(GSH) in biological samples was determined by high performance liquid chromatographic(HPLC) method with fluorescence detector(FLD) after monobromobimane(MBB) or 4-fluoro-7-sulfobenzofurazan(SBD-F) derivatization. The detection limit of $0.03{\mu}g/mL$ was obtained after MBB derivatization, derivative of MBB was about 200 times more sensitive than that of SBD-F. N-acetylcysteine was used as internal standard and tetrabutylammonium ion as counter ion for better separation. The determination by ion-pairing chromatography after MBB derivatization was characterized by linearity in the range between $0.08{\sim}8.33{\mu}g/mL$ with a good correlation coefficient of 0.998. By precision test appeared relative standard deviation at less than 5% at three different concentrations. This method can be used for the analysis of GSH in plasma and tissue.