• The Journal of Korean Medicine
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• v.38 no.4
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• pp.1-10
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• 2017

Objectives: The aim of the present study is to evaluate the effects of the extract of Pongamia pinnata on the morphology, viability, and differentiation potential of human stem cells derived from the gingiva. Methods: Stem cells obtained from gingivae were cultured in an osteogenic medium in the presence of methanol extract of Pongamia pinnata (PPT) at concentrations ranging from 0.001 to 1%. Evaluations of cell morphology and cellular viability were done at Day 1. Alkaline phosphatase activity assays and Alizarin red S staining were performed to evaluate the osteogenic differentiation of stem cells. Results: The morphology of stem cells in the presence of PPT at final concentrations of 0%, 0.001%, 0.01%, 0.1%, and 1% did not produce any noticeable changes when compared with the untreated control group. Application of PPT produced a significant increase in alkaline phosphatase activity when compared to the control group. The results of the Alizarin Red S staining showed a significant increase of absorbance with the 0.001% group. Conclusions: Based on these findings, it was concluded that PPT could produce beneficial effects on mesenchymal stem cells with enhanced osteogenic differentiation.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.45 no.8
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• pp.697-703
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• 2017

A teflon ionization modeling has been conducted to predict the performance of a PPT(Pulsed Plasma Thruster). One dimensional unsteady circuit model and Teflon ablation model were implemented. The Saha equation was adapted to predict the ionization of Carbon and Fluorine gas. The lumped circuit model including a resistance and a inductance model of a plasma was adapted to predict the magnitude of a discharge current. Numerical simulation results had good agreements with pervious research. The degree of current change according to PPT operating voltage was examined.

• Animal cells and systems
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• v.13 no.2
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• pp.205-212
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• 2009

Known as a female hormone, estrogen, performs important functions, and the activities of the hormone are mediated via the estrogen receptor. The principal objective of the present study was to assess the effects of a estrogen receptor agonist in male reproductive organs. In this study, the estrogen receptor alpha agonist, PPT, was injected subcutaneously into adult male mice. The effects of PPT on the murine reproductive system were histologically assessed at 3,5, and 8 weeks after treatment. In the treatment group, reductions were observed in the weight of the body, testis and epididymis. Microscopic examination revealed a reduction in seminiferous tubular diameter in the testis, and epithelial cell height in the epididymis during the experiment. 8 weeks after treatment, spermatogenesis was not detected, nor was the lumen of the seminiferous tubules. In the fertility test, 1 week after PPT injection, the fertilizing ability of males was decreased, and on the 2nd and 3rd weeks, complete infertility was observed. In conclusion, the injection of high concentrations of PPT into adult males induced physiological changes, including infertility, and also induced morphological changes, including a reduction in the height of epithelial cells within the reproductive system.

• Journal of International Academy of Physical Therapy Research
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• v.7 no.1
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• pp.956-964
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• 2016

The purpose of this study was to identify the effects of continuous muscle strengthening applied to the antagonist of the sternocleidomatoid, upper trapezius, and pectoralis major, which are the shortened muscles of forward head posture(FHP) subjects, and Evjenth-Hamberg stretching(EHS) applied to the shortened muscles on changes in pressure pain threshold(PPT). Twenty subjects were divided into the continuous antagonist strengthening(CAS) group(n=10) and the EHS group(n=10), and each group performed its respective exercise three times a week for a six week period. The results were as follows: The comparison of changes in PPT within each group before and after the treatment showed a statistically significant difference(p<.05) according to the treatment period and a statistically significant difference according to the treatment period and method(p<.05). While the comparison of the tests of between subjects effects between the groups did not show a statistically significant difference, the CAS group exhibited better effects. The above results suggest that the combined application of CAS and EHS generates better effects on changes in PPT than the single application of EHS. Given that stretching and muscle strengthening exercises even for the short research period of six weeks could change the PPT, continuous exercises and a correct postural habit for a longer period of time are likely to help prevent chronic pain and correct FHP.

• Journal of Aquaculture
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• v.17 no.4
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• pp.246-250
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• 2004

Survival of 12.7 g (average body weight) Litopenaeus vannamei was examined at temperatures of 14, 16, 18, 28, 32, 34 and 36$^{\circ}C$ and salinities of l0, 20, 30 and 40 ppt after 7 days. Hemolymph osmolality was measured at 12 combinations of salinities (l0, 20, 30 and 40 ppt) and temperature levels (18, 28 and 34$^{\circ}C$) after 14 days. The results show that the species have their best survival between temperatures of 18 and 3$0^{\circ}C$ and salinity above 20 ppt. Hemolymph osmolality increased with increased salinity at all temperatures tested. The isosmotic point calculated from the linear relationship between hemolymph osmolality and medium osmolality and recorded as 826, 809 and 1,117 mOsm/kg which is equivalent to 29.4, 28.8 and 40.2 ppt at 18, 28 and 34$^{\circ}C$, respectively. The slopes obtained from the relationship between hemolymph osmolality and medium osmolality were 0.314, 0.276 and 0.541 for L. vannamei at 18, 28 and 34$^{\circ}C$, respectively, suggesting that the shrimp at 34$^{\circ}C$ regulated osmotic concentration much worse than those at 18 and 28$^{\circ}C$. This result also indicated that L. vannamei living at 34$^{\circ}C$, showed more fluctuation in hemolymph osmolality than those at 18 and 28$^{\circ}C$.

• ALGAE
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• v.35 no.1
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• pp.79-89
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• 2020

Pythium species are ubiquitous organisms known to be pathogens to terrestrial plants and marine algae. While several Pythium species (hereafter, Pythium) are described as pathogens to marine red algae, little is known about the pathogenicity of Pythium on marine green algae. A strain of a Pythium was isolated from a taxonomically unresolved filamentous Ulva collected in an intertidal area of Oslo fjord. Its pathogenicity to a euryhaline Ulva intestinalis collected in the same area was subsequently tested under salinities of 0, 15, and 30 parts per thousand (ppt). The Pythium isolate readily infected U. intestinalis and decimated the filaments at 0 ppt. Mycelium survived on U. intestinalis filaments for at least 2 weeks at 15 and 30 ppt, but the infection did not progress. Sporulation was not observed in the infected algal filaments at any salinity. Conversely, Pythium sporulated on infected grass pieces at 0, 15, and 30 ppt. High salinity retarded sporulation, but did not prevent it. Our Pythium isolate produced filamentous non-inflated sporangia. The sexual stage was never observed and phylogenetic analysis using internal transcribed spacer suggest this isolate belongs to the clade B2. We conclude that the Pythium found in the Oslo fjord was a pathogen of U. intestinalis under low salinity.

• Physical Therapy Rehabilitation Science
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• v.3 no.2
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• pp.142-147
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• 2014

Objective: This study aims to investigate high energy and low energy extracorporeal shockwave therapy (ESWT) and which one is more effective for shoulder pain. Design: Single blind randomized controlled trial. Methods: Fifty two subjects with upper trapezius (UT) trigger point (TrP) participated in this study. They were allocated to high energy (n=26) and low energy group (n=26). This study applies ESWT and investigates the changes of pressure pain threshold (PPT) and visual analogue scale (VAS). The high and low energy groups received focused piezo electric type ESWT 4 Hz, 1,000 pulses and 0.351 and $0.092mJ/mm^2$ respectively. Outcome measures of PPT and pain was measured by algometer and pain VAS. These measurements were performed before and after treatment. Results: The PPT value was significantly increased in both groups after treatment (p<0.05) and VAS scores were significantly decreased after treatment in both groups (p<0.05). However, there were no significant differences between groups. Conclusions: ESWT is an effective treatment for the application of the UT TrP. Although there were significant effects of extracorporeal shock wave therapy on PPT and VAS scores, there were no signficant differences between high and low energy extracorporeal shock wave therapy.

• Physical Therapy Rehabilitation Science
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• v.11 no.3
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• pp.329-334
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• 2022

Objective: Low back pain (LBP) is a symptom that accounts for a large proportion of musculoskeletal pain. Among them, non-specific LBP (NSLBP) means that the pathological cause is unknown, and belongs to the most common LBP. Studies on the mechanism of muscle control in LBP are insufficient, and quantitative studies are needed. Design: Observational cross-sectional study design Methods: A Thirty participants with NSLBP symptoms were enrolled, and their pressure pain thresholds (PPT) and muscle and fat thickness were measured. Participants measured the paraspinal muscles (PM) of the thoracic and lumbar spine and medial hamstring (semitendinosus) on the dominant and non-dominant sides in the prone position. Results: Among the variables that were significant in the correlation analysis, PM of the thoracic and lumbar spine showed a significant relationship in the PPT ([thoracic spine PM]=1.141+0.912 [lumbar spine PM]). Also, there was a significant relationship between the lumbar spine PM in the PPT and the thoracic spine PM in the muscle thickness ([lumbar spine PM of PPT]=4.057+0.117 [thoracic spine PM of muscle thickness]) Conclusions: Although there was no muscle imbalance according to the dominant and non-dominant side, there is a correlation between the pressure pain threshold and the muscle thickness between the paraspinal muscles of the thoracic spine and the lumbar spine.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.8 no.3
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• pp.181-184
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• 1975

A aeries of experiments on the tolerance and growth of common carp, about 10 cm long in body length, in brackish water containing salinities of 2 to 20 ppt, were carried out in indoor aquariums. All fish well survived in waters containing salinities up to 12 ppt, when transfered directly from fresh water, and fairly well survived at 15 ppt, when acclimated at lower salinities before putting them into higher salinities. The temperature during the experiments was subjected to the room temperature ranging from $14^{\circ}\;to\;27^{\circ}C$. The food efficiency and growth rate were good both for alive Tubifex and pelletized dry feed at salinities from 2 to 8 ppt, but at 12 ppt the result was very poor for alive Tubifex while, for pellet feed, food efficiency was very good though growth rate was relatively low.

• The Korean Journal of Physiology and Pharmacology
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• v.16 no.2
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• pp.113-118
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• 2012

Ginsenosides are low molecular weight glycosides found in ginseng that exhibit neuroprotective effects through inhibition of $N$-methyl-D-aspartic acid (NMDA) receptor channel activity. Ginsenosides, like other natural compounds, are metabolized by gastric juices and intestinal microorganisms to produce ginsenoside metabolites. However, little is known about how ginsenoside metabolites regulate NMDA receptor channel activity. In the present study, we investigated the effects of ginsenoside metabolites, such as compound K (CK), protopanaxadiol (PPD), and protopanaxatriol (PPT), on oocytes that heterologously express the rat NMDA receptor. NMDA receptor-mediated ion current ($I_{NMDA}$) was measured using the 2-electrode voltage clamp technique. In oocytes injected with cRNAs encoding NMDA receptor subunits, PPT, but not CK or PPD, reversibly inhibited $I_{NMDA}$ in a concentration-dependent manner. The $IC_{50}$ for PPT on $I_{NMDA}$ was $48.1{\pm}4.6\;{\mu}M$, was non-competitive with NMDA, and was independent of the membrane holding potential. These results demonstrate the possibility that PPT interacts with the NMDA receptor, although not at the NMDA binding site, and that the inhibitory effects of PPT on $I_{NMDA}$ could be related to ginseng-mediated neuroprotection.