Browse > Article

Estrogen Receptor Alpha Agonist Propyl Pyrazole Triol Causes Alterations of the Morphology and Function of the Mouse Male Reproductive System  

Lee, Eun-Jung (Department of Biology, College of Life Sciences and Natural Resources, Sunchon National University)
Cho, Hyun-Wook (Department of Biology, College of Life Sciences and Natural Resources, Sunchon National University)
Publication Information
Animal cells and systems / v.13, no.2, 2009 , pp. 205-212 More about this Journal
Abstract
Known as a female hormone, estrogen, performs important functions, and the activities of the hormone are mediated via the estrogen receptor. The principal objective of the present study was to assess the effects of a estrogen receptor agonist in male reproductive organs. In this study, the estrogen receptor alpha agonist, PPT, was injected subcutaneously into adult male mice. The effects of PPT on the murine reproductive system were histologically assessed at 3,5, and 8 weeks after treatment. In the treatment group, reductions were observed in the weight of the body, testis and epididymis. Microscopic examination revealed a reduction in seminiferous tubular diameter in the testis, and epithelial cell height in the epididymis during the experiment. 8 weeks after treatment, spermatogenesis was not detected, nor was the lumen of the seminiferous tubules. In the fertility test, 1 week after PPT injection, the fertilizing ability of males was decreased, and on the 2nd and 3rd weeks, complete infertility was observed. In conclusion, the injection of high concentrations of PPT into adult males induced physiological changes, including infertility, and also induced morphological changes, including a reduction in the height of epithelial cells within the reproductive system.
Keywords
estrogen receptor; agonist; mouse; testis; epididymis; fertility;
Citations & Related Records

Times Cited By Web Of Science : 0  (Related Records In Web of Science)
Times Cited By SCOPUS : 0
연도 인용수 순위
  • Reference
1 Couse JF and Korach KS (1999) Estrogen receptor null mice: What have we learned and where will they lead us? Endocrine Rev 20: 358-417   DOI   ScienceOn
2 Goyal HO, Braden TD, Mansour M, Williams CS, Kamaleldin A, and Srivastava KK (2001) Diethylstilbestrol-treated adults rats with altered epididymal sperm numbers and sperm motility parameters, but without alterations in sperm production and sperm morphology. Biol Reprod 64: 927-934   DOI   ScienceOn
3 Hess RA, Bunick D, Lee KH, Bahr J, Korach KS, and Lubahn DB (1997) A role for oestrogens in the male reproductive system. Nature 390: 509-512   DOI   PUBMED   ScienceOn
4 O'Donnell L, Robertson KM, Jones ME, and Simpson ER (2001) Estrogen and spermatogenesis. Endocrine Reviews 22: 289-318   DOI   ScienceOn
5 Oliveira CA, Carnes K, Franca LR, and Hess RA (2001) Infertility and testicular atrophy in the antiestrogen-treated adult male rat. Biol Reprod 65: 913-920   DOI   ScienceOn
6 Pfaffl MW, Lange IG, and Meyer HHD (2003) The gastrointestinal tract as target of steroid hormone action: Quantification of steroid receptor mRNA expression (AR, ER$\alpha$, ER$\beta$ and PR) in 10 bovine gastrointestinal tract compartments by kinetic RT-PCR. J Steroid Biochem Mol BioI 84: 159-166   DOI   PUBMED   ScienceOn
7 Roesch DM (2006) Effects of selective estrogen receptor agonists on food intake and body weight gain in rats. Physiol Behavior 87: 39-44   DOI   PUBMED   ScienceOn
8 Stauffer SR, Coletta CJ, Tedesco R, Nishiguchi G, Carlson K, Sun J, Katzenellenbogen BS, and Katzenellenbogen JA (2000) Pyrazole ligands: Structure-affinity/activity relationships and estrogen receptor-$\alpha$-selective agonists. J Med Chem 43: 4934-4947   DOI   ScienceOn
9 Sun J, Huang YR, Harrington WR, Sheng S, Katzenellenbogen JA, and Katzenellenbogen BS (2002) Antagonists selective for estrogen receptor $\alpha$. Endocrinol 143: 941-947   DOI   PUBMED   ScienceOn
10 Toft D and Gorski J (1966) A receptor molecule for estrogens: Isolation from the rat uterus and preliminary characterization. PNAS 55: 1574-1581   DOI   ScienceOn
11 Cappon GD, Horimoto M, and Hurtt ME (2004) Reproductive toxicity assessment of lasofoxifene, a selective estrogen receptor modulator (SERM), in male rats. Birth Defects Res (Part B) 71: 142-149   DOI   ScienceOn
12 Taylor AH and Al-Azzawi F (2000) Immunolocalisation of oestrogen receptor beta in human tissues. J Mol Endocrinol 24: 145-155   DOI   ScienceOn
13 Carreau S, Genissel C, Bilinska B, and Levallet J (1999) Sources of oestrogen in the testis and reproductive tract of the male. Intl J Androl 22: 211-223   DOI   PUBMED
14 Oliveira CA, Mahecha GAB, Carnes K, Prins GS, Saunders PTK, Franca LR, and Hess RA (2004) Differential hormonal regulation of estrogen receptors ER$\alpha$ and ER$\beta$ and androgen receptor expression in rat efferent ductules. Reprod 128: 73-86   DOI   PUBMED   ScienceOn
15 Cho HW, Nie R, Carnes K, Zhou Q, Sharief NAQ, and Hess RA (2003) The antiestrogen ICI 182,780 induces early effects on the adult male mouse reproductive tract and long-term decreased fertility without testicular atrophy. Reprod Biol Endocrinol 1: 57   DOI   ScienceOn
16 Harris HA, Katzenellenbogen JA, and Katzenellenbogen BS (2002) Characterization ofthe biological roles of the estrogen receptors, ERa and ER$\beta$, in estrogen target tissues in vivo through the use of an ERa-selective ligand. Endocrinol 143: 4172-4177   DOI   ScienceOn
17 Kaneto M, Kanamori S, Hishikawa A, and Kishi K (1999) Epididymal sperm motion as a parameter of male reproductive toxicity: sperm motion, fertility, and histopathology in ethinylestradiol-treated rats. Reprod Toxicol 13: 279-289   DOI   ScienceOn
18 Wakeling AE, Newboult E, and Peters SW (1989) Effects of antiestrogens on the proliferation of MCF-7 human breast cancer cells. J Mol Endocrinol 2: 225-234   DOI   ScienceOn
19 Kraichely OM, Sun J, Katzenellenbogen JA, and Katzenellenbogen BS (2000) Conformational changes and coactivator recruitment by novel ligands for estrogen receptor-$\alpha$ and estrogen receptor-$\beta$. Correlations with biological character and distinct differences among SRC coactivator family members. Endocrinol 141: 3534-3545   DOI   PUBMED   ScienceOn
20 Kuiper GGJM, Enmark E, Pelto-Huikko M, Nilsson S, and Gustafsson J (1996) Cloning of a novel estrogen receptor expressed in rat prostate and ovary. PNAS 93: 5925-5930   DOI
21 Shiau AK, Barstad D, Loria PM, Cheng L, Kushner PJ, Agard DA, and Greene GL (1998) The structural basis of estrogen receptor/coactivator recognition and the antagonism of this interaction by tamoxifen. Cell 95: 927-937   DOI   ScienceOn
22 Janulis L, Bahr JM, Hess RA, Janssen S, Osawa Y, and Bunick D (1998) Rat testicular germ cells and epididymal sperm contain active P450 aromatase. J Androl 19: 65-71   PUBMED
23 Cook JC, Johnson L, O'Connor JC, Biegel LB, Krams CH, Frame SR, and Hurtt ME (1998) Effects of dietary 17 beta-estradiol exposure on serum hormone concentrations and testicular parameters in male Crl:CD BR rats. Toxicol Sci 44: 155-168   DOI   ScienceOn
24 Wade GN and Heller HW (1993) Tamoxifen mimics the effect of estradiol on food intake, body weight, and body composition in rats. Am J Physiol 264: R1219-1223
25 Hess RA (2003) Estrogen in the adult male reproductive tract: a review. Reprod Biol Endocrinol 1: 52   DOI   ScienceOn
26 Cignarella A, Paoletti R, and Puglisi L (2001) Direct Effects of estrogen on the vessel wall. Med Res Rev 21: 171-184   DOI   ScienceOn
27 Levallet J, Bilinska B, Mittre H, Genissel C, Fresnel J, and Carreaul S (1998) Expression and immunolocalization of functional cytochrome P450 aromatase in mature rat testicular cells. BioI Reprod 58: 919-926   DOI   ScienceOn
28 Zhou Q, Nie R, Prins GS, Saunders PTK, Katzenellenbogen BS, and Hess RA (2002) Localization of androgen and estrogen receptors in adult male mouse reproductive tract. J Androl 23 : 870-881   PUBMED
29 Han HJ, Jung JC, and Taub M (1999) Response of primary rabbit kidney proximal tubule cells to estrogens. J Cell Physiol 178: 35-43   DOI   ScienceOn
30 Atanassova N, McKinnell C, Turner KJ, Walker M, Fisher IS, Morley M, Millar MR, Groome NP, and Sharpe RM (2000) Comparative effects of neonatal exposure of male rats to potent and weak (environmental) estrogens on spermatogenesis at puberty and the relationship to adult testis size and fertility: evidence for stimulatory effects of low estrogen levels. Endocrinol 141: 3898-3907   DOI   ScienceOn
31 Sapino A, Pietribiasi F, Bussolati G, and Marchisio PC (1986) Estrogen- and tamoxifen-induced rearrangement of cytoskeletal and adhesion structures in breast cancer MCF-7 cells. Cancer Res 46: 2526-2531   PUBMED
32 Gill-Sharma MK, Dsouza S, Padwal V, Balasinor N, Aleem M, Parte P, and Juneja HS (2001) Antifertility effects of estradiol in adult male rats. J Endocrinol Invest 24: 598-607   PUBMED
33 Oliveira CA, Nie R, Carnes K, Franca LR, Prins GS, Saunders PTK, and Hess RA (2003) The antiestrogen ICI 182,780 decreases the expression of estrogen receptor-alpha but has no effect on estrogen receptor-beta and androgen receptor in rat efferent ductules. Reprod Biol Endocrinol 1: 75   DOI   ScienceOn
34 Nitta H, Bunick D, Hess RA, Janulis L, Newton SC, Millette CF, Osawa Y, Shizuta Y, Toda K, and Bahr JM (1993) Germ cells of the mouse testis express P450 aromatase. Endocrinol 132: 1396-1401   DOI   ScienceOn