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http://dx.doi.org/10.13048/jkm.17036

The effects of Pongamia pinnata on osteogenic differentiation and mineralization of human stem cells derived from the gingiva  

Lee, Hyunjin (Department of Periodontics, College of Medicine, The Catholic University of Korea)
Uddin, Md. Salah (Ethnobotanical Database of Bangladesh)
Kim, Yong-In (International Biological Material Research Center, Korea Research Institute of Bioscience and Biotechnology)
Choi, Sangho (International Biological Material Research Center, Korea Research Institute of Bioscience and Biotechnology)
Park, Jun-Beom (Department of Periodontics, College of Medicine, The Catholic University of Korea)
Publication Information
The Journal of Korean Medicine / v.38, no.4, 2017 , pp. 1-10 More about this Journal
Abstract
Objectives: The aim of the present study is to evaluate the effects of the extract of Pongamia pinnata on the morphology, viability, and differentiation potential of human stem cells derived from the gingiva. Methods: Stem cells obtained from gingivae were cultured in an osteogenic medium in the presence of methanol extract of Pongamia pinnata (PPT) at concentrations ranging from 0.001 to 1%. Evaluations of cell morphology and cellular viability were done at Day 1. Alkaline phosphatase activity assays and Alizarin red S staining were performed to evaluate the osteogenic differentiation of stem cells. Results: The morphology of stem cells in the presence of PPT at final concentrations of 0%, 0.001%, 0.01%, 0.1%, and 1% did not produce any noticeable changes when compared with the untreated control group. Application of PPT produced a significant increase in alkaline phosphatase activity when compared to the control group. The results of the Alizarin Red S staining showed a significant increase of absorbance with the 0.001% group. Conclusions: Based on these findings, it was concluded that PPT could produce beneficial effects on mesenchymal stem cells with enhanced osteogenic differentiation.
Keywords
cell differentiation; herbal medicine; Pongamia; stem cells;
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1 Jeong SH, Lee JE, Jin SH, Ko Y, Park JB. Effects of Asiasari radix on the morphology and viability of mesenchymal stem cells derived from the gingiva. Mol Med Rep. 2014;10:3315-3319.   DOI
2 Jeong SH, Kim BB, Lee JE, Ko Y, Park JB. Evaluation of the effects of Angelicae dahuricae radix on the morphology and viability of mesenchymal stem cells. Mol Med Rep. 2015;12:1556-1560.   DOI
3 Jeong SH, Lee JE, Kim BB, Ko Y, Park JB. Evaluation of the effects of Cimicifugae Rhizoma on the morphology and viability of mesenchymal stem cells. Exp Ther Med. 2015;10:629-634.   DOI
4 Ha DH, Pathak S, Yong CS, Kim JO, Jeong JH, Park JB. Potential differentiation ability of gingiva originated human mesenchymal stem cell in the presence of tacrolimus. Sci Rep. 2016;6:34910.   DOI
5 Maumus M, Jorgensen C, Noel D. Mesenchymal stem cells in regenerative medicine applied to rheumatic diseases: role of secretome and exosomes. Biochimie. 2013;95:2229-2234.   DOI
6 Zhang N, Kang T, Xia Y, Wen Q, Zhang X, Li H, et al. Effects of salvianolic acid B on survival, self-renewal and neuronal differentiation of bone marrow derived neural stem cells. Eur J Pharmacol. 2012;697:32-39.   DOI
7 Yang L, Wang NL, Cai GP. Maohuoside A promotes osteogenesis of rat mesenchymal stem cells via BMP and MAPK signaling pathways. Mol Cell Biochem. 2011;358:37-44.   DOI
8 Kim DR, Lee JE, Shim KJ, Cho JH, Lee HC, Park SK, et al. Effects of herbal Epimedium on the improvement of bone metabolic disorder through the induction of osteogenic differentiation from bone marrow-derived mesenchymal stem cells. Mol Med Rep. 2017;15: 125-130.   DOI
9 Qian K, Xu H, Dai T, Shi K. Effects of Tanshinone IIA on osteogenic differentiation of mouse bone marrow mesenchymal stem cells. Naunyn Schmiedebergs Arch Pharmacol. 2015; 388:1201-1209.   DOI
10 Cai M, Li G, Tao K, Yang Y, Lou L, Cai Z, et al. Maohuoside A acts in a BMP-dependent manner during osteogenesis. Phytother Res. 2013;27:1179-1184.   DOI
11 Kim SY, An SY, Lee JS, Heo JS. Zanthoxylum schinifolium enhances the osteogenic potential of periodontal ligament stem cells. In Vitro Cell Dev Biol Anim. 2015;51:165-173.   DOI
12 Li J, Jiang Z, Li X, Hou Y, Liu F, Li N, et al. Natural therapeutic agents for neurodegenerative diseases from a traditional herbal medicine Pongamia pinnata (L.) Pierre. Bioorg Med Chem Lett. 2015;25:53-58.   DOI
13 Al Muqarrabun LM, Ahmat N, Ruzaina SA, Ismail NH, Sahidin I. Medicinal uses, phytochemistry and pharmacology of Pongamia pinnata (L.) Pierre: a review. J Ethnopharmacol. 2013;150:395-420.   DOI
14 Brijesh S, Daswani PG, Tetali P, Rojatkar SR, Antia NH, Birdi TJ. Studies on Pongamia pinnata (L.) Pierre leaves: understanding the mechanism(s) of action in infectious diarrhea. J Zhejiang Univ Sci B. 2006;7:665-674.   DOI
15 Carcache-Blanco EJ, Kang YH, Park EJ, Su BN, Kardono LB, Riswan S, et al. Constituents of the stem bark of Pongamia pinnata with the potential to induce quinone reductase. J Nat Prod. 2003;66:1197-1202.   DOI
16 Yazdani-Nasab Z, Piravi-Vanak Z. A Study on the Specifications of Cold Pressed Colza Oil. Recent Pat Food Nutr Agric. 2015;7:47-52.   DOI
17 Jin SH, Lee JE, Yun JH, Kim I, Ko Y, Park JB. Isolation and characterization of human mesenchymal stem cells from gingival connective tissue. J Periodontal Res. 2015;50: 461-467.   DOI
18 Ha DH, Yong CS, Kim JO, Jeong JH, Park JB. Effects of tacrolimus on morphology, proliferation and differentiation of mesenchymal stem cells derived from gingiva tissue. Mol Med Rep. 2016;14:69-76.   DOI
19 Liu YR, Qu SX, Maitz MF, Tan R, Weng J. The effect of the major components of Salvia Miltiorrhiza Bunge on bone marrow cells. J Ethnopharmacol. 2007;111:573-583.   DOI
20 Park JB, Zhang H, Lin CY, Chung CP, Byun Y, Park YS, et al. Simvastatin maintains osteoblastic viability while promoting differentiation by partially regulating the expressions of estrogen receptors alpha. J Surg Res. 2012;174:278-283.   DOI
21 Jeong SH, Jiang Y, Guo H, Wang MH, Park JB. Anti-inflammatory and anticancer effects of methanol, ethanol and water extracts of Asiasarum heterotropoide. Biomed Rex. 2016;27:103-109.
22 Mukudai Y, Zhang M, Shiogama S, Kondo S, Ito C, Motohashi H, et al. Methanol and Butanol Extracts of Paeonia lutea Leaves Repress Metastasis of Squamous Cell Carcinoma. Evid Based Complement Alternat Med. 2016;2016: 6087213.
23 Wang MH, Jeong SH, Guo H, Park JB. Anti-inflammatory and cytotoxic effects of methanol, ethanol, and water extracts of Angelicae Dahuricae Radix. J Oral Sci. 2016; 58:125-131.   DOI
24 Wang F, Yu M, Yan X, Wen Y, Zeng Q, Yue W, et al. Gingiva-derived mesenchymal stem cell-mediated therapeutic approach for bone tissue regeneration. Stem Cells Dev. 2011;20: 2093-2102.   DOI
25 Chang NJ, Chen YW, Shieh DE, Fang HY, Shie MY. The effects of injectable calcium silicate-based composites with the Chinese herb on an osteogenic accelerator in vitro. Biomed Mat. 2015;10:055004.   DOI
26 Tomar GB, Srivastava RK, Gupta N, Barhanpurkar AP, Pote ST, Jhaveri HM, et al. Human gingiva-derived mesenchymal stem cells are superior to bone marrow-derived mesenchymal stem cells for cell therapy in regenerative medicine. Biochem Biophys Res Commun. 2010;393:377-383.   DOI
27 Li Z, Jiang CM, An S, Cheng Q, Huang YF, Wang YT, et al. Immunomodulatory properties of dental tissue-derived mesenchymal stem cells. Oral Dis. 2014;20:25-34.   DOI
28 Zhang QZ, Nguyen AL, Yu WH, Le AD. Human oral mucosa and gingiva: a unique reservoir for mesenchymal stem cells. J Dent Res. 2012;91:1011-1018.   DOI
29 Wang XL, Xie XH, Zhang G, Chen SH, Yao D, He K, et al. Exogenous phytoestrogenic molecule icaritin incorporated into a porous scaffold for enhancing bone defect repair. J Orthop Res. 2013;31:164-172.   DOI