• Title/Summary/Keyword: PAK2

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Effect of Water Temperature and Packing Type on Quality of Fresh-cut Pak-choi (세척 청경채의 선도 유지에 대한 세척수 온도와 포장 형태의 영향)

  • Kim, Byeong-Sam;Chang, Min-Sun;Park, Shin-Young;Cha, Hwan-Soo;Kwon, Ki-Hyun;Kim, Gun-Hee
    • Food Science and Preservation
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    • v.15 no.1
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    • pp.1-8
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    • 2008
  • Quality attributes of fresh-cut pak-choi (Brassica campestris var. chinensis) as affected by hydrocooling and packing were investigated in terms of weight loss, respiration, vitamin C content, total chlorophyll content, microbial load and sensory properties during storage at 4 and $10^{\circ}C$. Fresh pak-choi was trimmed and washed with cold water $(1^{\circ}C)$ as well as tap water $(6^{\circ}C)$ for 30 sec 3 times and then packaged in PP (polypropylene) film bag and PETE (polyethylene terephthalate) bay, and stored for 9 days at 4 and $10^{\circ}C$. Weight loss was decreased by washing and packing generally. Respiration rate was increased slowly over the storage at $4^{\circ}C$. Vitamin C content and total chlorophyll contents of pak-choi packaged within PETE bay decreased gradually during storage. Hydrocooling and packing within PETE bay treatments resulted in approximately 1-2 log CFU/g reduction of microbial load.

The Clinical Significance of STAT-PAK ULTRA FAST$^{(R)}$ and ICT Tuberculosis$^{(R)}$ for Serologic Diagnosis of Tuberculosis (폐결핵 진단을 위한 STAT-PAK ULTRA FAST$^{(R)}$와 ICT Tuberculosis$^{(R)}$의 유용성에 관한 연구)

  • Kim, Geun-Hwa;Park, Hee-Sun;Kim, Myung-Hoon;Kang, Dong-Won;Lee, Kyu-Seung;Ko, Dong-Seok;Suh, Jae-Chul;Jeong, Seong-Su;Kim, Ju-Ock;Kim, Sun-Young
    • Tuberculosis and Respiratory Diseases
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    • v.47 no.3
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    • pp.311-320
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    • 1999
  • Background: In recent years, tuberculosis has re-emerged as a major health problem in both industrialized & developing countries. Recent advances in identifying & purifying antigens secreted in active tuberculosis infection have lead to the development of serological assays based on a number of immunodominant antigens. To date, the most sensitive and specific of these antigens has been the 38-kDa antigen. Method: Two rapid membrane-based serologic assays using antigen(38-kDa) from mycobacterium tuberculosis for the diagnosis of tuberculosis were evaluated in 22 patients with smear-positive pulmonary tuberculosis, 14 patients with inactive pulmonary tuberculosis, and 9 patients with non-tuberculous lung disease. Result: The evaluation of validity(sensitivity, specificity, positive predictive value, negative predictive value, false positivity and false negativity) of STAT-PAK ULTRA FAST$^{(R)}$ were 77.3%, 28.6%, 63.0%, 44.4%, 71.4 %, and 22.7% for differential diagnosis of active pulmonary tuberculosis and inactive pulmonary tuberculosis, respectively. The evaluation of validity of STAT-PAK ULTRA FAST$^{(R)}$ were 77.3%, 33.3%, 73.9%, 37.5%, 66.7%, and 22.7% for differential diagnosis of active pulmonary tuberculosis and non-tuberculosis. The evaluation of validity of ICT Tuberculosis$^{(R)}$ were 54.5%, 57%, 66.7%, 44.4%, 42.9%, and 45.5% for differential diagnosis of active pulmonary tuberculosis and inactive pulmonary tuberculosis. The evaluation of validity of ICT Tuberculosis$^{(R)}$ were 54.5%, 100%, 100%, 47.4%, 0%, and 45.4% for differential diagnosis of active pulmonary tuberculosis and non-tuberculosis. Conclusion: We concluded no effectiveness of STAT-PAK ULTRA FAST$^{(R)}$ & ICT tuberculosis$^{(R)}$on serologic diagnosis of pulmonary tuberculosis. In the future, further large-scale study should be needed for serologic diagnosis of pulmonary tuberculosis.

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Production of Monoclonal Antibody about Specific Key Enzyme of Hyoscyamine $6{\beta}-Hydroxylase$ (H6H) in Scopolia parviflora

  • Kang, Young-Min;Jung, Hee-Young;Kang, Seung-Mi;Jin, Byung-Rae;Lee, Sang-Chul;Lee, Byung-Hyun;Choi, Myung-Suk
    • Korean Journal of Medicinal Crop Science
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    • v.12 no.2
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    • pp.135-140
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    • 2004
  • Total RNAs were isolated from cultured roots of Scopolia parviflora, $poly(A)^+$ RNA was obtained through the mRNA purification, cDNA library of Hnh6h was constructed. Recombinant baculoviruses in Spodoptera frugiperda (Sf) cells were constructed by use of the transfer vector pBacPAK, which has the AcNPV sequence under the polyhedrin promoter. The expression vector carrying Hnh6h gene was transferred to S. parviflora and obtained transgenic hairy root lines. Our results confirmed the over expression of the H6H protein was used by anti-pBacPAK about cDNAs of S. parviflora. This study will served for production of tropane alkaloids by metabolic engineering.