• Title/Summary/Keyword: PAIR

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Development of SSLP Marker Targeted to P34 Null Gene in Soybean (콩 P34 단백질 결핍 유전자를 이용한 SSLP 마커 개발)

  • Yang, Kiwoung;Ko, Jong-Min;Lee, Young-Hoon;Jeon, Myeong Gi;Jung, Chan-Sik;Baek, In-Youl;Kim, Hyun-Tae;Park, Keum-Yong
    • Korean Journal of Breeding Science
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    • v.42 no.5
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    • pp.502-506
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    • 2010
  • Soybean seed possesses about 15 allergenic proteins recognized by IgEs from soy-sensitive human. The allergenic impact of soybean proteins limit its extensive usage in a broad range of processed foods. Soybean protein P34 or Gly m Bd 30k of the cysteine protease family is one of the major allergen of the soybean seed. P34-null soybean, PI567476, was identified among soybean (Glycine max & Glycine soja Sieb. and Zucc) of approximately 16,226 accessions from USDA soybean germplasm screened. Also, for P34 gene (Williams 82; whole genome sequence cultivar) and P34 null gene (PI567476) comparative analysis of sequences listed in the NCBI database showed the presence of a SSLP (Simple Sequence Length Polymorphism) of 4 base pair. So, a SSLP marker was designed to reveal the polymorphism of the locus. In this study, a population of 339 $F_2$ recombinant inbred lines generated by cross between Taekwang (Glycine max) and PI567476 was used to select $F_{2:3}$ plant of a P34 null gene. The result separation rate Taekwang type, heterozygous type and PI567476 type were shown in 85: 187: 67 since single gene is concerned in as the separation rate of 1:2:1 in $X^2{_{0.05}}=5.99$, df=2. In future, selected plant will identify protein level, whether P34 null protein is equal to P34 null gene.

Estimation of the Optimal Harvest and Stock Assessment of Hairtail Caught by Multiple Fisheries (다수어업의 갈치 자원평가 및 최적어획량 추정)

  • Nam, Jongoh;Cho, Hoonseok
    • Ocean and Polar Research
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    • v.40 no.4
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    • pp.237-247
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    • 2018
  • This study aims to estimate optimal harvests, fishing efforts, and stock levels of hairtail harvested by the large pair bottom trawl, the large otter trawl, the large purse seine, the offshore long line, and the offshore angling fisheries by using the surplus production models and the current value Hamiltonian method. Processes of this study are as follows. First of all, this study estimates the standardized fishing efforts regarding the harvesting of the hairtail by the above five fishing gears based on the general linear model developed by Gavaris. Secondly, this study estimates environmental carrying capacity (k), intrinsic growth rate (r), and catchability coefficient (q) by applying the Clarke Yoshimoto Pooley (CY&P) model among various surplus production models. Thirdly, this study estimates the optimal harvests, fishing efforts, and stock levels regarding the hairtail by the current value Hamiltonian method, including the average landing price, the average unit cost, and the social discount rate. Finally, this study attempts a sensitivity analysis to figure out changes in optimal harvests, fishing efforts, and stock levels due to changes in the average landing price and the average unit cost. As results induced by the current value Hamiltonian method, the optimal harvests, fishing efforts, and stock levels regarding the hairtail caught by several fishing gears were estimated as 33,133 tons, 901,080 horse power, and 79,877 tons, respectively. In addition, from the results of the sensitivity analysis, first of all, if the average landing price of the hairtail constantly increases, the optimal harvests of it increase at a decreasing rate, and then harvests finally slightly decrease as a result of decreases in stock levels. Secondly, if the average unit cost of fishing efforts continuously increases, the optimal fishing efforts decreases, but optimal stock levels increase. Optimal harvests start climbing and then decrease continuously due to increases in the average unit cost. In summary, this study suggests that the optimal harvests (33,133 tons) were larger than actual harvests (25,133 tons), but the optimal fishing efforts (901,080 horse power) were much less than estimated standardized fishing efforts (1,277,284 horse power), corresponding to the average of the recent three years (2014-2016). This result implies that the hairtail has been inefficiently harvested and recently overfished due to excessive fishing efforts. Efficient management and conservation policies on stock levels need to be urgently implemented. Some appropriate strategies would be to include the hairtail in the Korean TAC species or to extend the closed fishing season for this species.

A Study on the Effect Analysis and Improvement of Cardiopulmonary Resuscitation on Life-rafts (구명뗏목에서의 비상대응 심폐소생술의 효과 분석 및 개선에 관한 연구)

  • Lee, Chang-Woo
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.25 no.4
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    • pp.433-440
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    • 2019
  • Offshore working environments such as ships, offshore oil and gas plants, and offshore wind turbines are isolated and directly exposed to rough seas, which pose high risks of safety accidents. Therefore, all workers in offshore plants should be able to cope with emergency situations and must be qualified according to relevant laws and regulations such as the International Convention on Standards of Training, Certification and Watchkeeping for Seafarers (STCW Convention) and Offshore Petroleum Industry Training Organization (OPITO) standards. In particular, marine workers should be able to perform cardiopulmonary resuscitation (CPR) in isolated locations or enclosed and confined spaces such as those in life-boats, life-rafts, rescue-boats, etc. Because the floor material is made of rubber, it may be difficult to perform chest compressions in life-rafts used to escape from emergency situations in ships or offshore plants. Chest compressions performed on life-rafts may reduce the accuracy of CPR and increase fatigue for those providing aid. To measure the accuracy and fatigue of those performing CPR in life-rafts, 15 experimenters with more than five years of experience as first aid instructors were exposed to different CPR environments in a marine safety training center equipped with an artificial wave generator. The results showed that the accuracy of CPR in the classroom was 99.6 %, but that in various life-raft environments was only 84 %. T-verification of the two sites confirmed the reduced accuracy of CPR performed on life-rafts. CPR on life-rafts should be performed in groups of two and with the use of automated chest compression devices.

Effect of Sensory Integration Group Therapy on Sensory Processing, Peer Interaction and Task Performance of Children With Intellectual Disabilities (그룹감각통합치료가 지적장애아동의 감각처리와 또래상호작용, 과제수행에 미치는 영향)

  • Choi, Ji-Hyon;Kim, Hee;Lee, Jae-Shin;Yoo, Doo-Han
    • Korean Journal of Occupational Therapy
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    • v.26 no.4
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    • pp.111-125
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    • 2018
  • Objective : This study was designed to determine the effects of sensory integration group therapy with regard to the sensory processing, peer interactions, and task performance, and whether they continue. Methods : Twelve children aged 7 to 13 years enrolled in this study for 60 minutes per session, three times a week for 8 weeks. Sensory integration group therapy consisted of a total of 24 activities that included sensory processing, play skills, and interaction with peers. Short Sensory Profile was used to select subjects. In order to measure the outcome, we used the Short Sensory Profile, Penn Interactive Peer Play Scale and Canadian Occupational Performance Measure at pre, post, and follow-up evaluations. The results were analyzed by means of repeated measures analysis, and the pre-test, post-test, and follow-up tests were compared using the Wilcoxon matched-pair signed rank test. Results : After sensory integration group therapy, sensory processing, peer interaction, and task performance significantly improved(p<.05). In addition, we confirmed that the effects of treatment were maintained in the 4 weeks follow-up test. Conclusion : Sensory integration group therapy is an effective way of mediating effects not only by improving sensory processing skills, but also by providing imitation and training in groups for children in need of peer interaction and linking them to daily life.

Highly-permeable Mixed Matrix Membranes Based on SBS-g-POEM Copolymer, ZIF-8 and Ionic Liquid (SBS-g-POEM 공중합체, ZIF-8, 이온성 액체에 기반한 고투과성 혼합 매질 분리막)

  • Kang, Dong A;Kim, Kihoon;Kim, Jong Hak
    • Membrane Journal
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    • v.29 no.1
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    • pp.44-50
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    • 2019
  • In this paper, we developed mixed matrix membranes (MMMs) consisting of SBS-g-POEM block-graft copolymer, ionic liquid (EMIMTFSI) and ZIF-8 nanoparticles to separate a $CO_2/N_2$ gas pair. The SBS-g-POEM is a rubbery block-graft copolymer synthesized through low-cost free-radical polymerization. The EMIMTFSI was dissolved into the SBS-g-POEM matrix and solution synthesized ZIF-8 nanoparticles were also dispersed into the copolymer matrix. The physico-chemical properties of manufactured membranes were characterized by Fourier transform infrared spectroscopy (FT-IR), differential scanning calorimetry (DSC), scanning electron microscopy (SEM), X-ray diffraction (XRD), which showed that the components were compatible with each other. The gas separation performance was confirmed by time-lag measurements showing $CO_2$ permeability of 537.0 barrer and $CO_2/N_2$ selectivity of 15.2. The result represents the EMIMTFSI and ZIF-8 nanoparticles improves the gas permeability more than two-times, without significantly sacrificing the $CO_2/N_2$ selectivity.

Rotordynamic Performance Measurements and Predictions of a FCEV Air Compressor Supported on Gas Foil Bearings (가스 포일 베어링으로 지지되는 연료전지 전기자동차용 공기압축기의 회전체동역학적 성능 측정 및 예측)

  • Hwang, Sung Ho;Moon, Chang Gook;Kim, Tae Ho;Lee, Jongsung;Cho, Kyung Seok;Ha, Kyoung-Ku;Lee, Chang Ha
    • Tribology and Lubricants
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    • v.35 no.1
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    • pp.44-51
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    • 2019
  • The paper presents the rotordynamic performance measurements and model predictions of a fuel cell electric vehicle (FCEV) air compressor supported on gas foil bearings (GFBs). The rotor has an impeller on one end and a thrust runner on the other end. The front (impeller side) and rear (thrust side) gas foil journal bearings (GFJBs) are located between the impeller and thrust runner to support the radial loads, and a pair of gas foil thrust bearings are located on both sides of the thrust runner to support the axial loads. The test GFJBs have a partial arc shim foil installed between the top foil and bump strip layers to enhance hydrodynamic pressure generation. During the rotordynamic performance tests, two sets of orthogonally installed eddy-current displacement sensors measure the rotor radial motions at the rotor impeller and thrust ends. A series of speed-up and coast-down tests to 100k rpm demonstrates the dominant synchronous (1X) rotor responses to imbalance masses without noticeable subsynchronous motions, which indicates a rotordynamically stable rotor-GFB system. Finite element analysis of the rotor determines the rotor free-free (bending) natural modes and frequencies well beyond the maximum rotating frequency. The predicted damped natural frequencies and damping ratios of the rotor-GFB system reveal rotordynamic stability over the speeds of interest. The imbalance response predictions show that the predicted critical speeds and rotor amplitudes strongly agree with the test measurements, thus validating the developed rotordynamic model.

Bird accidents in Southern Mongolia: a case study of bird electrocution (몽골 남부지역의 야생조류 사고: 감전사를 중심으로)

  • Ganbold, Onolragchaa;Bing, Gi-Chang;Purevee, Erdenetushig;Munkhbayar, Munkhbaatar;Choi, Won-Suk;Jargalsaikhan, Ariunbold;Paik, In-Hwan;Purevdorj, Zoljargal;Jargal, Namsrai;Paek, Woon-Kee
    • Korean Journal of Ornithology
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    • v.25 no.2
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    • pp.94-100
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    • 2018
  • Bird electrocution on contact with electricity lines is well reported and is quite common in Mongolian open areas. We visited 15-kV electricity distribution pole lines in a Southern Mongolian semi-desert region three times in 2017, in April, July, and September, to assess their risks to birds. The carcasses of 45 electrocuted birds representing 12 species were identified from 250 poles (overall mortality rate of 1.12% every 10 km). The majority of these electrocuted birds were endangered Saker Falcon (n=11) and common Black Kite (n=11). The lacks of roosting or perching sites in our study sites (indeed other open areas in Mongolia) may resulted for such high rate bird electrocution. A 751-base pair (bp) cytochrome c oxidase I (COI) gene was amplified for DNA sequence-based identification of carcasses that were difficult to identify directly. Our findings revealed the high electrocution risk for birds in Mongolian open areas, especially for the birds of prey, with relatively low-efficiency electrocution mitigation approaches. The findings also indicate that there is a need for better understanding of the risk of bird electrocution, particularly in the open areas. This will contribute to the conservation of endangered species.

Precise Measurements of the Along-track Surface Deformation Related to the 2016 Kumamoto Earthquakes via Ionospheric Correction of Multiple-Aperture SAR Interferograms (다중개구간섭영상의 이온층 보정을 통한 2016 구마모토 지진의 비행방향 지표변위 정밀 관측)

  • Baek, Won-Kyung;Jung, Hyung-Sup
    • Korean Journal of Remote Sensing
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    • v.34 no.6_4
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    • pp.1489-1501
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    • 2018
  • In 2016 Kumamoto, Japan, the foreshocks of $M_j$ 6.5 and 6.4, mainshock of $M_j$ 7.3 besides more than 2,000 aftershocks occurred in succession. Large surface deformation occurred due to this serial earthquakes and three-dimensional measurements of the deformation have been presented for the study of fault structures (Baek, 2017). The 3d measurements retrieved from two ascending pairs (20160211_20160602, 20151119_20160616) and a descending pair (20160307_20160418) acquired from ALOS PALSAR-2. In order to avoid mixing ionospheric error components on along-track surface deformation, the descending multiple-aperture interferogram, which do not contain the deformation of aftershocks after 20160418, was utilized. For these reason, there was a temporal discrepancy of about 2 months in extracting the north-south deformation. In this study, we applied a directional filter based ionospheric correction to ascending multiple-aperture interferograms, in order to reduce this discrepancy and understand more accurate fault movements. As a result of the ionospheric correction, an additional displacement signal was observed nearby fault lines. The root-mean-squared errors compared to GPS were about 9.87, 8.13 cm respectively. These results show improvements of 4.8 and 6.4 times after ionospheric correction. We expected that these along-track measurements would be used to decide more accurate movements of faults related to the 2016 Kumamoto Earthquake.

A Method to Improve Matching Success Rate between KOMPSAT-3A Imagery and Aerial Ortho-Images (KOMPSAT-3A 영상과 항공정사영상의 영상정합 성공률 향상 방법)

  • Shin, Jung-Il;Yoon, Wan-Sang;Park, Hyeong-Jun;Oh, Kwan-Young;Kim, Tae-Jung
    • Korean Journal of Remote Sensing
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    • v.34 no.6_1
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    • pp.893-903
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    • 2018
  • The necessity of automatic precise georeferencing is increasing with the increase applications of high-resolution satellite imagery. One of the methods for collecting ground control points (GCPs) for precise georeferencing is to use chip images obtained by extracting a subset of an image map such as an ortho-aerial image, and can be automated using an image matching technique. In this case, the importance of the image matching success rate is increased due to the limitation of the number of the chip images for the known reference points such as the unified control point. This study aims to propose a method to improve the success rate of image matching between KOMPSAT-3A images and GCP chip images from aerial ortho-images. We performed the image matching with 7 cases of band pair using KOMPSAT-3A panchromatic (PAN), multispectral (MS), pansharpened (PS) imagery and GCP chip images, then compared matching success rates. As a result, about 10-30% of success rate is increased to about 40-50% when using PS imagery by using PAN and MS imagery. Therefore, using PS imagery for image matching of KOMPSAT-3A images and aerial ortho-images would be helpful to improve the matching success rate.

A Study of Stone Sculptures of Jagneung and Sareung - The Reinstatement and Stone Sculpture Simplification - (장릉(莊陵)과 사릉(思陵)의 석물 연구 - 추봉과 석물 간소화를 중심으로 -)

  • Kim, Yi Soon
    • Korean Journal of Heritage: History & Science
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    • v.45 no.1
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    • pp.34-51
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    • 2012
  • Jangneung (the Royal Tomb of King Danjong) and Sareung (the Royal Tomb of Queen Jeongsun) are simple in their design and layout. Not only are the sizes of the stone statues small, but stone soldiers are not installed at all despite the fact that Danjong at one point served as the King of Joseon. Moreover, only a pair of seoksu (stone animal) is installed for each Tomb. These royal tombs were originally made as common tombs and later upgraded as Royal Tombs when Danjong's royal status was reinstated posthumously in 1698. Thus, in comparison to royal tombs in general, the scale of these tombs is by far modest. For this paper, archival records and other literature for the burial upgrade process and the stone sculptures are examined; also, the stone sculptures have been examined and measured on-site, and they have been compared and analyzed based on the data thus obtained. Furthermore, this paper traces the historical context of the period in which the simplification of the stone sculptures took place for Jangneung and Sareung. The paper argues that the cause of the modest scale lies not so much in the fact that King Danjong had been dethroned as a commoner, but in the cataclysmic famine and epidemic that swept the country during the time when the two royal tombs were upgraded as such.