Korean Journal of Breeding Science (한국육종학회지)

The Korean Society of Breeding Science (한국육종학회)
권호 표지

Development of SSLP Marker Targeted to P34 Null Gene in Soybean

콩 P34 단백질 결핍 유전자를 이용한 SSLP 마커 개발

  • 양기웅 (국립식량과학원 기능성작물부) ;
  • 고종민 (국립식량과학원 기능성작물부) ;
  • 이영훈 (국립식량과학원 기능성작물부) ;
  • 전명기 (국립식량과학원 기능성작물부) ;
  • 정찬식 (국립식량과학원 기능성작물부) ;
  • 백인열 (국립식량과학원 기능성작물부) ;
  • 김현태 (국립식량과학원 기능성작물부) ;
  • 박금룡 (국립식량과학원 기능성작물부)
  • Received : 2010.11.18
  • Published : 2010.12.31
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Abstract

Soybean seed possesses about 15 allergenic proteins recognized by IgEs from soy-sensitive human. The allergenic impact of soybean proteins limit its extensive usage in a broad range of processed foods. Soybean protein P34 or Gly m Bd 30k of the cysteine protease family is one of the major allergen of the soybean seed. P34-null soybean, PI567476, was identified among soybean (Glycine max & Glycine soja Sieb. and Zucc) of approximately 16,226 accessions from USDA soybean germplasm screened. Also, for P34 gene (Williams 82; whole genome sequence cultivar) and P34 null gene (PI567476) comparative analysis of sequences listed in the NCBI database showed the presence of a SSLP (Simple Sequence Length Polymorphism) of 4 base pair. So, a SSLP marker was designed to reveal the polymorphism of the locus. In this study, a population of 339 $F_2$ recombinant inbred lines generated by cross between Taekwang (Glycine max) and PI567476 was used to select $F_{2:3}$ plant of a P34 null gene. The result separation rate Taekwang type, heterozygous type and PI567476 type were shown in 85: 187: 67 since single gene is concerned in as the separation rate of 1:2:1 in $X^2{_{0.05}}=5.99$, df=2. In future, selected plant will identify protein level, whether P34 null protein is equal to P34 null gene.

콩 알레르기에 민감한 사람들은 15가지가 넘는 콩 알레르기 단백질을 인지한다. 이러한 알레르기 단백질 때문에 콩의 광범위한 사용이 제한적이다. 시스테인 프로테아제에 속하는 P34 단백질은 콩의 주된 알러젠이다. 미국 국무부에서 16,226개의 유전자원에서 P34 단백질이 결핍된 PI567476 유전자원을 찾아냈다. P34 유전자 염기서열과 P34 유전자가 결실된 염기서열을 NCBI 데이터베이스에서 확인한 결과 P34 유전자가 결실된 염기서열에서 4 bp 가 삽입되었다는 것을 확인하였고, 그 부위에서 SSLP 마커를 개발하였다. 본 연구에서는 태광콩과 PI567476을 이용한 교배조합 $F_2$ 339개체를 개발한 분자표지마커로 확인하였다. 실험결과 태광콩 유형과 heterozygous 유형 및 PI567476 유형의 분리비는 85: 187: 67로 $X^2{_{0.05}}=5.99$, df=2에서 1:2:1의 분리비로 하나의 유전자가 관여한다는 것으로 나타났다. 앞으로 P34 단백질 관련 분자마커가 단백질 수준에서 정확히 일치하는지 확인 할 것이다.

Keywords

Acknowledgement

Supported by : 국립식량과학원

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