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Development of SSLP Marker Targeted to P34 Null Gene in Soybean  

Yang, Kiwoung (Department of Functional Crop, NICS, RDA)
Ko, Jong-Min (Department of Functional Crop, NICS, RDA)
Lee, Young-Hoon (Department of Functional Crop, NICS, RDA)
Jeon, Myeong Gi (Department of Functional Crop, NICS, RDA)
Jung, Chan-Sik (Department of Functional Crop, NICS, RDA)
Baek, In-Youl (Department of Functional Crop, NICS, RDA)
Kim, Hyun-Tae (Department of Functional Crop, NICS, RDA)
Park, Keum-Yong (Department of Functional Crop, NICS, RDA)
Publication Information
Korean Journal of Breeding Science / v.42, no.5, 2010 , pp. 502-506 More about this Journal
Abstract
Soybean seed possesses about 15 allergenic proteins recognized by IgEs from soy-sensitive human. The allergenic impact of soybean proteins limit its extensive usage in a broad range of processed foods. Soybean protein P34 or Gly m Bd 30k of the cysteine protease family is one of the major allergen of the soybean seed. P34-null soybean, PI567476, was identified among soybean (Glycine max & Glycine soja Sieb. and Zucc) of approximately 16,226 accessions from USDA soybean germplasm screened. Also, for P34 gene (Williams 82; whole genome sequence cultivar) and P34 null gene (PI567476) comparative analysis of sequences listed in the NCBI database showed the presence of a SSLP (Simple Sequence Length Polymorphism) of 4 base pair. So, a SSLP marker was designed to reveal the polymorphism of the locus. In this study, a population of 339 $F_2$ recombinant inbred lines generated by cross between Taekwang (Glycine max) and PI567476 was used to select $F_{2:3}$ plant of a P34 null gene. The result separation rate Taekwang type, heterozygous type and PI567476 type were shown in 85: 187: 67 since single gene is concerned in as the separation rate of 1:2:1 in $X^2{_{0.05}}=5.99$, df=2. In future, selected plant will identify protein level, whether P34 null protein is equal to P34 null gene.
Keywords
P34 null gene; Soybean allergy; SSLP marker; MAS(Marker Assisted Selection);
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