• Applied Biological Chemistry
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• v.41 no.7
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• pp.489-495
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• 1998

The optimum culture condition of Scopulariopsis brevicaulis for the production of ${\alpha}$-galactosidase was as follows: Tryptone 1.5%, $NH_4NO_3$ 0.2%, Raffinose 2.5%, $KH_2PO_4$ 0.5%, yeast extract 0.5%, pH 7.0, $27^{\circ}C$. The optimum pH and temperature for the enzyme activity of ${\alpha}$-galactosidase producing Scopulariopsis brevicaulis were pH 7.0 and $27^{\circ}C$, respectively. The enzyme was relatively stable at $pH\;6.0{\sim}8.0$ and at temperature below $40^{\circ}C$. The activity of the enzyme was inhibited by $Ag^{2+},\;Hg^{2+},\;Cu^{2+}$, p-chloromercuribenzoic acid and Iodine. These results would indicate the presence of -SH groups in the catalytic site of the enzyme. Km value was 1.9 mM for $p-nitrophenyl-{\alpha}-D-galactopyranoside$ and Vmax value was $9.66{\times}10^2\;{\mu}M/min$. Sugar constituents of culture broth were identified by HPLC that the enzyme liberated sucrose, glucose and fructose from raffinose and raffinose was significantly decreased.

• Journal of Digital Convergence
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• v.20 no.2
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• pp.573-583
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• 2022

This study was attempted to analyze the effect of a note-taking strategy applied to blended learning adult nursing education. Data were collected from September 17 to October 15, 2021, and analyzed using the SPSS/WIN 26.0 program with a single group pre/post design targeting 33 nursing students. As a main result, learning motivation rose from 3.27 before class to 3.40 after class, but there was no significant difference(t=-1.501, p=.143), and class participation significantly increased from 2.95 before class to 3.27 after class(t=-2.669, p=.012). Motivation for learning before class was pre-class participation(r=.838, p<.001), learning motivation after class(r=.545, p=.001), and class participation after class(r=.462, p=.007) and each showed a significant positive correlation. Learning motivation and class participation according to gender(Wilks'𝛌=.866), class interest(Wilks'𝛌=.632), and class satisfaction(Wilks'𝛌=.822) were all higher than the significance level of .05, so they were no significant difference. Based on these results, applying a note-taking strategy to blended learning adult nursing education was effective in improving class participation. In a future study, it is necessary to verify the effectiveness by applying a note-taking strategy customized according to academic achievement.

• Journal of Physiology & Pathology in Korean Medicine
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• v.22 no.5
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• pp.1250-1255
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• 2008

Onchungeum(OCE), a herbal formula, has been used for treatment of anemia, discharging blood and skin diseases. In the previous study, we investigated the anti-cancer effect of OCE by G1 arrest of the cell cycle in human hepatocarcinoma cells, Hep3B cells. In this study, it was examined that the difference of anti-proliferative mechanisms by change in the ratio of OCE composition (OCE I) in Hep3B cells. Treatment of OCE I exhibited a relatively strong anti-proliferative activity and caused various morphological changes such as membrane shrinkage and cell floating. In addition, OCE-I arrests the cell cycle at G1 phase, which was associated with the down-regulation of cyclin D1 and Cdk6 expressions. The G1 arrest was also associated with the induction of Cdk inhibitors p27 and p21. Moreover, both p21 and p27 were detected by immunoprecipitation with anti-Cdk4 and anti-Cdk2 antibodies in OCE I-treated cells but in case of OCE, p21 did not make any complexes with Cdk4 and Cdk2. These results suggest that the change in the ratio of OCE composition might induce different mechanisms in anti-cancer efficacy of OCE, which may confer characteristic principles in oriental medical formula.

• Journal of Korean Clinical Nursing Research
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• v.29 no.1
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• pp.1-11
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• 2023

Purpose: The purpose of this study was to identify the influencing effects of job stress, professional autonomy, and reciprocity on the job embeddedness among comprehensive nursing care unit nurses. Methods: The participants in this study were 147 nurses who have worked for over 6 months in Comprehensive Nursing Care Unit. Data were collected from January 3 to January 31, 2022 from six general hospitals with more than 300 beds in three cities in G, G and P. Results: As the results of hierarchical regression analysis, job embeddedness was lower when nurses had clinical experiences for 1 to under 5 years (β=-.49, p<.001), 5 to under 10 years (β=-.27, p=.035), 10 to under 20 years (β=-.54, p<.001) compared to those who had clinical experiences for more than 20 years. Also, job embeddedness was higher when there was greater balance within team caregiving of reciprocality (β=.27, p<.001) and intrinsic reward (β=.22, p=.003), and lower role conflict (β =-.27, p<.001). Conclusion: The results of the study showed that job embededness would increase if the role conflict of comprehensive nursing care unit nurses reduces, if there was a culture that the members can make cooperative relationship with other health care professionals and, if there was an appropriate intrinsic reward depending on their work experience.

• Journal of Oral Medicine and Pain
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• v.44 no.1
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• pp.25-30
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• 2019

Purpose: The exact causes of burning mouth syndrome (BMS) is unclear so far. There are many studies to elucidate the relation between oral disease and genetic predisposition. In this study, we first tried to investigate salivary exosomal genetic components that could play an important role for diagnosing and elucidating the progression of BMS. Methods: We compared salivary exosomal micro RNAs (miRNAs) of BMS Patients to those of control using next generation sequencing (NGS). Unstimulated whole saliva from 15 patients with BMS and 10 control subjects were divided into two sets. Isolated exosomes and their total RNAs were subject to NGS for the screening of miRNAs. Results: There were up-regulated 10 exosomal miRNAs (hsa-miR-1273h-5p, hsa-miR-1273a, hsa-miR-1304-3p, hsa-miR-4449, hsa-miR-1285-3p, hsa-miR-6802-5p, hsa-miR-1268a, hsa-miR-1273d, hsa-miR-1273f, and hsa-miR-423-5p) and down-regulated 18 exosomal miRNAs (hsa-miR-27b-3p, hsa-miR-16-5p, hsa-miR-186-5p, hsa-miR-142-3p, hsa-miR-141-3p, hsa-miR-150-5p, hsa-miR-374a-5p, hsa-miR-93-5p, hsa-miR-29c-3p, hsa-miR-29a-3p, hsa-miR-148a-3p, hsa-miR-22-3p, hsa-miR-27a-3p, hsa-miR-424-5p, hsa-miR-19b-3p, hsa-miR-99a-5p, hsa-miR-548d-3p, and hsa-miR-19a-3p) in BMS patients comparing with those of control subjects. Conclusions: We show that there are 28 differential expression of miRNAs between the patients with BMS and those of control subjects. The specific function of indicated miRNAs should be further elucidated.

• Journal of the Chungcheong Mathematical Society
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• v.27 no.1
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• pp.1-8
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• 2014

Recently, q-Dedekind-type sums related to q-Euler polynomials was studied by Kim in [T. Kim, Note on q-Dedekind-type sums related to q-Euler polynomials, Glasgow Math. J. 54 (2012), 121-125]. It is aim of this paper to consider a p-adic continuous function for an odd prime to inside a p-adic q-analogue of the higher order Dedekind-type sums with weight related to modified q-Euler polynomials with weight by using Kim's p-adic q-integral.

• Journal of Korean Public Health Nursing
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• v.27 no.2
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• pp.267-279
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• 2013

Purpose: This study was conducted to identify blood pressure control rate and related factors in hypertensive patients. Methods: Data were collected using face to face survey with measuring blood pressure from 268 hypertensive patients. Results: Subjects without spouses were 2.19 times more likely to control their blood pressures (p=.002). Whenever subjects came up 1 score in the low sodium diet score, they were 1.37 times more likely to control their blood pressures (p=.044). The possibility of blood pressure control rose 1.58 times per point in the stress management score (p=.011) and the sleep and rest score (p=.002). Conclusion: It is important to develop education and intervention program of lifestyle regarding low sodium diet, stress management and sleep and rest, in order to improve the blood pressure control.

• Journal of environmental and Sanitary engineering
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• v.17 no.3
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• pp.1-6
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• 2002

This study was carried out to investigate the distribution of Legionella spp and microbe from cooling towers water of Public establishments in Seoul and Gyeonggi-Do. As results of this study, the cooling tower 132 sites were detecting L. pneumophila in order to July(12%) > August (14%) > June, September no-detected. The public establishments were detecting L. pneumophila in order to Department store(27.3%) > Hospital(8.7%) > Office(5.9%) > Big Mart(5.0%) > Hotel, Subway no-detected. The correlation coefficient pH and L.pneumophila showed 0.62(p=0.05), 0.27(p=0.45) in temperature and L.pheumophila, 0.40(p=0.25) in turbidity and L.pneumophila.

• Journal of Veterinary Clinics
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• v.3 no.1
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• pp.265-276
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• 1986

Pathological study was made of 173 gallbladders among 652 slaughter pigs. The size and the pH of the bile juice were made of 50 among 173 gallbladders with lesions and 50 normal gallbladders as control. The results are summerized as follows : 1. The histological lesions were shown hyperemia and congestion(83.2%), Iymphocytic infiltration(37.5%), Iympoid hyperplasia(27%), epithelial downgrowth(27%), erosion and ulcer(22%), papillary hyperplasia (20.2%), pericholecystic edema(19.5%), hemorrhage(11%), and leukemic cell infiltration(0.6%). 2. In the gallbladders with lesions mean size was 4.26${\times}$7.88cm in diameter and mean pH is 7.2. In the control means size and mean pH were 4.57${\times}$8.13cm in diameter and 7.1. The difference of size between gallbladders with lesions and these as control was not statistically significant. The pH of bile juice between two showed also no statistically significant difference.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.27 no.3
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• pp.151-155
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• 2014

We have developed quantum dot light emitting diodes (QD-LEDs) using a InP/ZnSe/ZnS multi-shell QD emission layer. The hybrid structure of organic hole transport layer/QD/organic electron transport layer was used for fabricating QD-LEDs. Poly(4-butylphenyl-diphenyl-amine) (poly-TPD) and tris[2,4,6-trimethyl-3-(pyridin-3-yl)phenyl]borane (3TPYMB) molecules were used as hole-transporting and electron-transporting layers, respectively. The emission, current efficiency, and driving characteristics of QD-LEDs with 50, 65 nm thick 3TPYMB layers were investigated. The QD-LED with a 50 nm thick 3TPYMB layer exhibited a maximum current efficiency of 1.3 cd/A.