• Title/Summary/Keyword: P242

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Differential display RT-PCR 기법을 이용한 돼지 등심조직의 품종 간 발현차이 유전자의 연구

  • Kim, Nam-Guk;Jo, Jung-Ho;Im, Jong-Hyeon;Bang, Gyeong-Jeong;Song, Min-Jin;Park, Beom-Yeong;Kim, Eon-Hyeon;Lee, Chang-Su
    • Proceedings of the Korean Society for Food Science of Animal Resources Conference
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    • 2005.05a
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    • pp.239-242
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    • 2005
  • 본 연구는 성장 속도 및 서로 다른 육질 특성을 지닌 돼지 품종을 이용하여, 육질 및 성장에 관련된 유전자원을 확보하고, 이를 이용한 유전 육종의 기초 자료를 제공하기 위하여 수행하였다. Differential display (DD) RT-PCR 기법을 통해 돼지 품종 간 발현 차이를 보이는 유전자인 NADH dehydrogenase 1과 ATPase 6를 동정하였다. 동정된 유전자의 발현량 분석을 위한 RT-PCR 결과, 각 유전자의 발현량이 재래돼지에서 외래 품종 (랜드레이 스 및 요크셔)에 비해 2배 이상 높음을 확인 할 수 있었다 (p<0.01). 이러한 발현차이 유전자를 이용하여 육질과의 관련성 연구 및 유전자의 기능에 대한 연구가 지속되어야 할 것이다.

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NONLOCAL FRACTIONAL DIFFERENTIAL INCLUSIONS WITH IMPULSE EFFECTS AND DELAY

  • ALSARORI, NAWAL A.;GHADLE, KIRTIWANT P.
    • Journal of the Korean Society for Industrial and Applied Mathematics
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    • v.24 no.2
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    • pp.229-242
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    • 2020
  • Functional fractional differential inclusions with impulse effects in general Banach spaces are studied. We discuss the situation when the semigroup generated by the linear part is equicontinuous and the multifunction is Caratheodory. First, we define the PC-mild solutions for functional fractional semilinear impulsive differential inclusions. We then prove the existence of PC-mild solutions for such inclusions by using the fixed point theorem, multivalued properties and applications of NCHM (noncompactness Hausdorff measure). Eventually, we enhance the acquired results by giving an example.

An Efficient Method to Prepare PCR Cloning Vectors

  • Hong, Soon-Gyu;Choi, Ji-Young;Pryor, Barry M.;Lee, Hong-Kum
    • Mycobiology
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    • v.37 no.3
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    • pp.240-242
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    • 2009
  • An improved procedure for preparing PCR cloning vectors was developed. This procedure includes the incorporation of adapters to create XcmI restriction enzyme sites in pBluescript II SK(+) vectors, digestion with XcmI followed by further digestion of the small fragment produced by XcmI digestion with additional enzymes, and purification with PCR purification kits. Using this procedure, PCR cloning vectors with high ligation efficiencies and low blue or false-positive colonies were obtained.

The Influence of Smoking and Low Dose Radiation Exposure to the Damage of the Lymphocyte DNA (흡연과 낮은 방사선 피폭량이 Lymphocyte DNA 손상에 미치는 영향)

  • Shin Heuyn-Kil;Kim Yun-Joo;Kwon Eun-Hye;Yook Jin-Young;Choi Soo-Yong
    • Environmental Analysis Health and Toxicology
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    • v.18 no.4
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    • pp.237-242
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    • 2003
  • Single cell gel electrophoresis (SCGE) was used to the experiment with the variation on the amount of smoking and low dose radiation exposure to find how much the Lymphocyte DNA was damaged, and especially for whom smoke a lot(about 20 or more than 20 cigarettes a day) it was found to be highly damaged. While, the damage of 'not more than 20 cigarettes a day' was found to be not so much significant as like for whom smoke about or more than 20 cigarettes a day And, according to the different amount of the radiation exposure, the Lymphocyte DNA was found to be considerably damaged for 0-13m Sv (P<0.01), it was not able to prove the relationship between the DNA damage and the radiation exposure.

A Synthesis and Characteristics for ZrO2-Y2O3 System Powders by Coprecipitation Method ; I. The Properties and Sinterabilities of ZrO2-8m/o Y2O3 (공침법에 의한 ZrO2-Y2O3계 분말합성 및 특성;I. ZrO2-8m/o Y2O3의 특성 및 소결성)

  • 방대규;윤종석;이희수
    • Journal of the Korean Ceramic Society
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    • v.26 no.2
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    • pp.242-248
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    • 1989
  • The powder properties and sinterability of ZrO2-8m/o Y2O3 prepared by coprecipitation were investigated. The specific surface area and the total pore volume were increased with increasing pH of sediment and using of ethyl alcohol for washing. The powders of prepared by freeze drying method were showed the smallest particle size and lowest agglomerate rate, and the powders obtained from spray drying method were showed porous structure. At 130$0^{\circ}C$, their relative density were 94-96%, so these sintered bodies have revealed high sinterability in spite of low sintering temperature.

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Agrobacterium-mediated Transformation of Rice 'Ilmibyeo' using HPT Selection Maker Gene

  • Guo, Jia;Cho, Joon-Hyeong;Jo, Hye-Jeong;Seong, Eun-Soo;Wang, Myeong-Hyeon
    • Korean Journal of Plant Resources
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    • v.20 no.3
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    • pp.242-246
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    • 2007
  • This study was conducted to produce the transgenic plant of rice. We obtained Agrobacterium AGL1 harbaring pCambial 300 vector with HPT gene. We carried out PCR analysis of 22 ea putative transgenic rice to investigate transformed lines. The 3 ea transgenic lines were detected insertion of HPT gene. Transgenic lines selected from PCR analysis were performed by Southern blot. From Southern blot, we obtained that two transgenic lines detected single band. We are going to study the method improving of cotransformation as well as transformation efficiency in rice.

Environmentally Assisted Cracking of Alloys at Temperatures near and above the Critical Temperature of Water

  • Watanabe, Yutaka
    • Corrosion Science and Technology
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    • v.7 no.4
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    • pp.237-242
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    • 2008
  • Physical properties of water, such as dielectric constant and ionic product, significantly vary with the density of water. In the supercritical conditions, since density of water widely varies with pressure, pressure has a strong influence on physical properties of water. Dielectric constant represents a character of water as a solvent, which determines solubility of an inorganic compound including metal oxides. Dissociation equilibrium of an acid is also strongly dependent on water density. Dissociation constant of acid rises with increased density of water, resulting in drop of pH. Density of water and the density-related physical properties of water, therefore, are the major governing factors of corrosion and environmentally assisted cracking of metals in supercritical aqueous solutions. This paper discusses importance of "physical properties of water" in understanding corrosion and cracking behavior of alloys in supercritical water environments, based on experimental data and estimated solubility of metal oxides. It has been pointed out that the water density can have significant effects on stress corrosion cracking (SCC) susceptibility of metals in supercritical water, when dissolution of metal plays the key role in the cracking phenomena.

A STUDY OF RELATIONSHIPS BETWEEN MIDSAGITTAL PLANE AND DENTAL MID LINE (정중시상면과 치아중심선과의 관계에 관한 연구)

  • Jin, Yong-Hwan
    • The Journal of the Korean dental association
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    • v.13 no.5
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    • pp.457-461
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    • 1975
  • The author observed the relationships beween the dental midline and the midsagittal plane by taking 242 cases P-A cephalo-graphy grouped by male and female 2-6 years age group, 7-15 years age group, and adult age group. The following results were obtained by the observation. 1. Generally, the median line almost coincided with dental midline ineach age group. 1. It showed som degree of deviation in each age group. 2. It showed some degree of deviation in each age group. 3. The some degree of deviation shifted in accordance with each age group. 4. In adult age group, the dental milline more coincided with median line in male than in female.

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Characterization of Al:ZnO thin films deposited at different substrate temperatures (기판 온도변화에 따른 Al-ZnO 박막의 특성)

  • No, I.J.;Shin, P.K.;Lee, C.;Kim, Y.H.;Ji, S.H.;Lim, Y.C.;Chung, M.Y.
    • Proceedings of the KIEE Conference
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    • 2007.07a
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    • pp.242-243
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    • 2007
  • Highly transparent conducting aluminum-doped zinc oxide (AZO) thin films were deposited on Corning glass substrate using an Nd:YAG pulsed laser deposition technology. AZO thin films deposited with 650nm thickness showed the best electrical properties of the electrical resistivity of $4.6{\times}10^{-4}[{\Omega}{\cdot}cm]$, a carrier concentration of $9.3{\times}10^{20}[cm^{-3}]$, and a carrier mobility of $31[cm^2/V{\cdot}s]$. Besides, the optical transmittance spectra in visible region (200-800nm) of AZO thin films show an high average transmittance over 90%.

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