Mycobiology

  • 제37권3호
  • /
  • Pages.240-242
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  • 2009
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  • 1229-8093(pISSN)
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  • 2092-9323(eISSN)
한국균학회 (The Korean Society of Mycology)
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An Efficient Method to Prepare PCR Cloning Vectors

  • Hong, Soon-Gyu (Polar BioCenter, Korea Polar Research Institute, KORDI) ;
  • Choi, Ji-Young (Polar BioCenter, Korea Polar Research Institute, KORDI) ;
  • Pryor, Barry M. (Division of Plant Pathology and Microbiology, Department of Plant Sciences, College of Agriculture and Life Sciences, University of Arizona) ;
  • Lee, Hong-Kum (Polar BioCenter, Korea Polar Research Institute, KORDI)
  • 발행 : 2009.09.30
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초록

An improved procedure for preparing PCR cloning vectors was developed. This procedure includes the incorporation of adapters to create XcmI restriction enzyme sites in pBluescript II SK(+) vectors, digestion with XcmI followed by further digestion of the small fragment produced by XcmI digestion with additional enzymes, and purification with PCR purification kits. Using this procedure, PCR cloning vectors with high ligation efficiencies and low blue or false-positive colonies were obtained.

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참고문헌

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  2. Chuang, S. E., Wang, K. C. and Cheng, A. L. 1995. Single-step direct cloning of PDC products. Trends Genet. 11:7-8 https://doi.org/10.1016/S0168-9525(00)88976-3
  3. Holton, T. A. and Graham, M. W. 1991. A simple and efficient method for direct cloning of PCR products using ddT-tailed vectors. Nucleic Acids Res. 19:1156 https://doi.org/10.1093/nar/19.5.1156
  4. Kovalic, D., Kwak, J. H. and Weisblum, B. 1991. General method for direct cloning of DNA fragments generated by the polymerase chain reaction. Nucleic Acids Res. 19:4560 https://doi.org/10.1093/nar/19.16.4560
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