• Title/Summary/Keyword: P123

Search Result 1,339, Processing Time 0.029 seconds

Studies on the Production of Thermostable Amylase. Part 1. Optimal Culture Conditions and Purification of Enzyme. (내열성 Amylase의 생산에 관한 연구 (제1보) 최적배양조건과 효소의 정제)

  • 오두환;이강표;변유량;유주현
    • Microbiology and Biotechnology Letters
    • /
    • v.9 no.2
    • /
    • pp.91-97
    • /
    • 1981
  • A thermophilic soil isolate Bacillus sp. Y-127 was selected for the production of thermostable amylase. The strain was used for the enzyme production and the thermostable amylase was characterized. The optimum cultural conditions for the enzyme production were 6$0^{\circ}C$ at pH 7.0 for 32 hours using a mineral medium containing 2% soluble starch and 0.2% yeast extract. The extra-cellular enzyme was purified about 123-folds with about 6% recovery. The purified enzyme was stable at pH between 4.0 and 7.0, and temperature up to 6$0^{\circ}C$.

  • PDF

Effects of gum elastic bougie in intubation with difficult airway (마네킹을 이용한 어려운 기도에서 부지 기관 내 삽관의 효과)

  • Shim, Gyu-Sik;Bang, Sung-Hwan;Ahn, Hee-Jeong
    • The Korean Journal of Emergency Medical Services
    • /
    • v.24 no.2
    • /
    • pp.123-131
    • /
    • 2020
  • Purpose: This study aimed to find out the effectiveness of gum elastic bougies for intubation in comparison to stylet according to airway type using a manikin. Methods: The study subjects were 52 paramedic students who intubated using a Macintosh laryngoscope and compared stylet and gum elastic bougie use in a 7.5 mmID endotracheal tube, on a manikin with either normal or difficult airway. Difficult airway was made Philadelphia neck collar. Collected data included intubation time, accuracy and ease of intubation, later analyzed by frequency analysis, descriptive analysis, independent t-test, chi square test, paired t-test, and McNemar test using SPSS Statistics 18.0. Results: There was a significant difference in intubation time according to intubation device and airway type (p=.000). There was no significant difference in accuracy of intubation according to intubation device or airway type (normal airway p=1.000, difficult airway p=.052). There was a significant difference in ease of intubation scale according to intubation device and airway type (p=.000, p=.000). Conclusion: Based on the speed and ease of intubation, gum elastic bougie is recommended for intubation in patients with difficult airways such as those with cervical injury.

Function of mORF1 Protein as a Terminal Recognition Factor for the Linear Mitochondrial Plasmid pMLP1 from Pleurotus ostreatus

  • Kim, Eun-Kyoung;Roe, Jung-Hye
    • Journal of Microbiology
    • /
    • v.37 no.4
    • /
    • pp.229-233
    • /
    • 1999
  • The mitochondrial plasmid pMLP1 from a white-rot fungus, Pleurotus ostreatus, is a double-stranded DNA containing 381 bp terminal inverted repeat (TIR) whose 5'-ends are covalently bound by terminal proteins. The plasmid contains two major open reading frames (ORFs), encoding putative DNA and RNA polymerases, and a minor ORF encoding a small, highly basic protein. To identify the DNA binding activity that recognizes the TIR region of pMLP1, gel retardation assays were performed with mitochondrial extracts. A specific protein binding to a region between 123 and 248 nt within TIR was observed. We examined whether the gene product of mORF1 bindes to this region specifically. E. coli cell extract which contains an overproduced mORF1 protein formed a complex specific to the region between 123 and 248 nt. Inclusion of mORF1 protein in the specific complex formed between P. ostreatus mitochondrial extract and TIR was confirmed by a supershift assay using polyclonal antibodies against the mORF1 protein. Our result suggest that the product of mORF1 may function as a terminal region recognition factor (TRF), recognizing an internal region in TIR.

  • PDF

Dopamine Transporter Density Assessed with $[^{123}I]IPT$ SPECT Before and After Risperidone Treatment in Children with Tourette's Disorder (뚜렛 장애 아동에서 Risperidone 치료 전후 $[^{123}I]IPT$ SPECT로 측정한 기저 신경절 도파민 운반체 밀도)

  • Kim, Tae-Hoon;Ryu, Won-Gee;Park, Se-Young;Chi, Dae-Yoon;Choi, Tae-Hyun;Lee, Kyung-Yul;Cheon, Keun-Ah;Yun, Mi-Jin;Kim, Jai-Keun;Lee, Jong-Doo;Ryu, Young-Hoon
    • The Korean Journal of Nuclear Medicine
    • /
    • v.38 no.1
    • /
    • pp.41-51
    • /
    • 2004
  • PURPOSE: Tourette's disorder (TD), which is characterized by multiple waxing and waning motor tics and one or more vocal tics, is known to be associated with abnormalities in the dopaminergic system. To testify our hypothesis that risperidone would improve tic symptoms of TD patients through the change of the dopaminergic system, we measured the dopamine transporter (DAT) densities between drug-naive children with TD and normal children, and investigated the DAT density before and after treatment with risperidone in drug-naive children with TD, using iodine-123 labelled $N-(3-iodopropen-2-yl)-2{\beta}-carbomethoxy-3beta-(4-chlorophenyl)tropane$ ($[^{123}I]IPT$) single photon omission computed tomography (SPECT). MATERIALS and METHODS: $[^{123}I]IPT$ SPECT Imaging and Yale Global Tic Severity Scale-Korean version (YGTSS-K) for assessing the tic symptom severity were carried out before and after treatment with risperidone for 8 weeks in nine drug-naive children with TD. Eleven normal children also underwent SPECT imaging 2 hours after an intravenous administration of $[^{123}I]IPT$. RESULTS: Drug-naive children with TD had a significantly greater increase in the specific/nonspecific DAT binding ratio of both basal ganglia compared with the normal children. However, no significant difference in the specific/nonspecific DAT binding ratio of the basal ganglia before and after treatment with risperidone in children with TD was found, although tic symptoms were significantly improved with risperidone. CONCLUSION: These findings suggest that DAT densities are directly associated with the pathophysiology of TD, however, that the effect of risperidone on tic symptoms in children with TD is not attributed to the change of dopaminergic system.

First Record of Paciforchestia pyatakovi (Crustacea: Amphipoda: Talitridae) from Korea

  • Kim, Min-Seop;Min, Gi-Sik
    • Animal Systematics, Evolution and Diversity
    • /
    • v.27 no.2
    • /
    • pp.123-130
    • /
    • 2011
  • The primitive beachflea, Paciforchestia pyatakovi (Derzhavin, 1937) living in gravel beaches was previously reported only from Japan and Russia. This species can be easily distinguished from another species in the same genus, P. klawei (Bousfield, 1961), by the degradation of all plepods and both rami that each consist of single article bearing 2-4 distal plumose setae. Descriptions of the diagnostic characteristics of the species are presented.

Factors Influencing Self-leadership in Nursing Students (간호대학생의 셀프리더십에 미치는 영향요인)

  • Kim, Doo Ree;Lim, HyoNam;Kim, Jin-il
    • Journal of the Korea Convergence Society
    • /
    • v.11 no.3
    • /
    • pp.369-377
    • /
    • 2020
  • Self-leadership is a positive factor for the organization and individuals, and it is a necessary competency for nursing students. The purpose of present study was to examine the factors affecting self-leadership. 173 nursing students in D city were collected and analyzed through a structured questionnaire. Self-leadership of nursing students had a positive correlation with creativity (r=.546, p<.001), self-assertion (r=.217, p=.004) and self-esteem (r=.258, p=.001). In addition, the influencing factors for self-leadership of nursing students were gender(β=.212, p=.001), grade(β=.123, p=.060), interpersonal relationship 'very good'(β=.327,p=.007), 'good'(β=.395, p=.023), 'moderate' (β=.328, p=.040) and creativity(β=.578, p<.001). Explanatory power was 35.5%. It is noteworthy to consider creativity and interpersonal relationship in developing strategy to improve self-leadership of nursing students.

A Convergence Study on the Factors Affecting Teacher Efficacy among School Teachers in COVID- 19 Special Disaster Area (코로나바이러스감염증-19 특별재난 일개 지역 교사의 교사 효능감에 영향을 미치는 요인 융합 연구)

  • Kim, Eunl-Hwi;Ha, Young-Sun;Park, Young-Kyung
    • Journal of Digital Convergence
    • /
    • v.19 no.1
    • /
    • pp.239-248
    • /
    • 2021
  • This study was aimed to investigate the factors influencing teacher efficacy of depression, anxiety, stress among school teachers in COVID- 19 special disaster area. The data were collected from May 9 to 16, 2020 for 123 high school teachers in D city. As a result of the study, the influential factor of teacher efficacy was satisfaction with duty (β=0.27, p=.002), economic level (β=0.18, p=.022), education (β=0.18, p=.022), subjective health state (β=0.16, p=.047), stress (β=-0.16, p=.044), gender(β=0.16, p=.042). These factors accounted for 35% in teacher efficacy. It was found that depression(β=-0.09, p=.468) and anxiety(β=-0.12, p=.320) had no significant effect on teacher efficacy. When developing a program to improve teacher efficacy, it is required to prepare measures not only to manage stress but also to improve job satisfaction.

Effect of Oil on Pool Boiling of Refrigerant on Enhanced Tubes having Different Pore Sizes (다공도가 다른 전열촉진관의 냉매 풀비등에 미치는 오일의 영향)

  • Kim Nae-Huyn;Lee Eung-Ryul;Min Chang-Keun
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
    • /
    • v.18 no.3
    • /
    • pp.254-261
    • /
    • 2006
  • The effect of enhanced geometry (pore diameter, gap width) is investigated on the pool boiling of R-123/oil mixture for the enhanced tubes having pores with connecting gaps. Tubes with different pore diameters (and corresponding gap widths) are specially made. Significant heat transfer degradation by oil is observed for the present enhanced tubes. At 5% oil concentration, the degradation is 26 to 49% for $T_{sat}=4.4^{\circ}C$. The degradation increases 50 to 67% for $T_{sat}=26.7^{\circ}C$. The heat transfer degradation is significant even with small amount of oil (20 to 38% degradation at 1% oil concentration for $T_{sat}=4.4^{\circ}C$), probably due to the accumulation of oil in sub-tunnels. The pore size (or gap width) has a significant effect on the heat transfer degradation. The maximum degradation is observed for $d_p$ = 0.20 mm tube at $T_{sat}=4.4^{\circ}C$, and for $d_p$=0.23 mm tube at $T_{sat}=26.7^{\circ}C$. The minimum degradation is observed for $d_p$=0.27 mm tube for both saturation temperatures. It appears that the oil removal is facilitated for the larger pore diameter (along with larger gap) tube. The highest heat transfer coefficient with oil is obtained for $d_p$ =0.23 mm tube, which yielded the highest heat transfer coefficient for pure R-123. The heat transfer degradation increases as the heat flux decreases.