• Title/Summary/Keyword: P.I.

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Characteristics of the R plasmid pKU10 isolated from Pseudomonas putida (Pseudomonas putida에서 분리한 플라스미드 pKU 10의 특성)

  • 임영복;이영록
    • Korean Journal of Microbiology
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    • v.25 no.4
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    • pp.282-289
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    • 1987
  • The characteristics of the plasmid pKU10 isolated from Pseudomonas putida KU816 were investigated and its restriction map was constructed. The pKU10 plasmid was a small R plasmid carrying genes for resistance to ampicillin, tetracyclin, and chloramphenicol, and cured by treatment with mitomycin C. The molecular size of pKU10 was estimated to be 9.4Kb. Pseudomonas strains and E. coli cells could be transformed for antibiotic resistance characters specified by pKU10 plasmid DNA. By incompatibility test with other plasmids, pKU10 is grouped into IncP-1. EcoRI, XhoI, SalI, BglII, and SmaI cleaved pKU10 once, while PstI cleaved at two sites, and HindIII cleaved at six sites. The restriction map was constructed by partial and complete digestion of the purified plasmid DNA with single, double, or triple restriction enzymes. Thus, pKU10 is expected to be used for a cloning vector in Pseudomonas cells.

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AN ANALOGUE OF THE HILTON-MILNER THEOREM FOR WEAK COMPOSITIONS

  • Ku, Cheng Yeaw;Wong, Kok Bin
    • Bulletin of the Korean Mathematical Society
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    • v.52 no.3
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    • pp.1007-1025
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    • 2015
  • Let $\mathbb{N}_0$ be the set of non-negative integers, and let P(n, l) denote the set of all weak compositions of n with l parts, i.e., $P(n,l)=\{(x_1,x_2,{\cdots},x_l){\in}\mathbb{N}^l_0\;:\;x_1+x_2+{\cdots}+x_l=n\}$. For any element $u=(u_1,u_2,{\cdots},u_l){\in}P(n,l)$, denote its ith-coordinate by u(i), i.e., $u(i)=u_i$. A family $A{\subseteq}P(n,l)$ is said to be t-intersecting if ${\mid}\{i:u(i)=v(i)\}{\mid}{\geq}t$ for all $u,v{\epsilon}A$. A family $A{\subseteq}P(n,l)$ is said to be trivially t-intersecting if there is a t-set T of $[l]=\{1,2,{\cdots},l\}$ and elements $y_s{\in}\mathbb{N}_0(s{\in}T)$ such that $A=\{u{\in}P(n,l):u(j)=yj\;for\;all\;j{\in}T\}$. We prove that given any positive integers l, t with $l{\geq}2t+3$, there exists a constant $n_0(l,t)$ depending only on l and t, such that for all $n{\geq}n_0(l,t)$, if $A{\subseteq}P(n,l)$ is non-trivially t-intersecting, then $${\mid}A{\mid}{\leq}(^{n+l-t-l}_{l-t-1})-(^{n-1}_{l-t-1})+t$$. Moreover, equality holds if and only if there is a t-set T of [l] such that $$A=\bigcup_{s{\in}[l]{\backslash}T}\;A_s{\cup}\{q_i:i{\in}T\}$$, where $$A_s=\{u{\in}P(n,l):u(j)=0\;for\;all\;j{\in}T\;and\;u(s)=0\}$$ and $$q_i{\in}P(n,l)\;with\;q_i(j)=0\;fo\;all\;j{\in}[l]{\backslash}\{i\}\;and\;q_i(i)=n$$.

Cloning of Genes for the Biosynthesis of Glutathione from E. coIi K-12 (E.coli K-12 균주로부터 글루타치온 합성 유전자의 클로닝)

  • 남용석;박영인;이세영
    • Microbiology and Biotechnology Letters
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    • v.19 no.6
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    • pp.575-582
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    • 1991
  • To increase the production of glutathione by the expression of recombinant gsh plasmids, two genes responsible for the biosynthesis of glutathione were isolated and cloned. To clone a gshI gene, the GS903 mutant strain, which is deficient in $\gamma$-glutamylcysteine synthetase activity, has been raised. A gshI gene was cloned using pBR322 plasmid as a 3.6 Kb PstI DNA fragment isolated from E. coli K-12 chromosomal DNA. Also a gshIl gene was cloned using pUC13 plasmid as a 2.2 Kb PstI-BamHI DNA fragment. To study the effects of plasmid copy number and passenger DNA size on the expression levels of the gsh genes, various recombinant plasmids containing different sets of genes were constructed. The expression levels of the gsh genes were increased approximately twice higher in pUC series plasmids than that in pBR322 plasmid. But the sizes of the passenger DNA containing the gsh genes in the vector plasmid did not affect on the expression levels of the gsh genes.

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A Study on the Recent Changes of Level of Club Cover in P & I Insurance (P & I 보험의 보상한도에 관한 고찰 - 최근의 변화 및 쟁점을 중심으로 -)

  • Shin, Gun-Hoon
    • THE INTERNATIONAL COMMERCE & LAW REVIEW
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    • v.22
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    • pp.201-226
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    • 2004
  • P & I Clubs are mutual and non-profit making insurers which offer shipowners cover for the contractual and third-party liabilities. Whereas most shipowners obtain P & I insurance to cover for their legal liabilities, they also obtain hull insurance to cover against damages to the hull of their vessels from commercial hull insurers. P & I insurance was distinguished from hull insurance in respect that it offered non-limited cover to shipowner member, but there was a serious debate between P & I Clubs in respect of the non-limited cover. A compromise by International Group of P & I Clubs eventually emerged under which, with effect from 20 February 1997, a financial cap was placed on the obligation of each shipowner to pay catasrophe calls to his club(20% of each ship's property limitation fund under 1976 Limitation Convention). Nevertheless many shipowners felt that this new cap on their potential catastrophe call had been set still too high, while others resisted any reduction in the figure established by the compromise. In the Meantime, the European Commission issued a Statement of Objections in June 1997, in which it indicated its objections with a compulsory single limit common th all the Group clubs as high as the 1997 compromise. Eventually the board of all the Group clubs decided that the figure of 20% of the Limitation Convention per ship property funds should be dropped down to 2.5% from 20 February 1999.

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Numerical studies of information about elastic parameter sets in non-linear elastic wavefield inversion schemes (비선형 탄성파 파동장 역산 방법에서 탄성파 변수 세트에 관한 정보의 수치적 연구)

  • Sakai, Akio
    • Geophysics and Geophysical Exploration
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    • v.10 no.1
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    • pp.1-18
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    • 2007
  • Non-linear elastic wavefield inversion is a powerful method for estimating elastic parameters for physical constraints that determine subsurface rock and properties. Here, I introduce six elastic-wave velocity models by reconstructing elastic-wave velocity variations from real data and a 2D elastic-wave velocity model. Reflection seismic data information is often decoupled into short and long wavelength components. The local search method has difficulty in estimating the longer wavelength velocity if the starting model is far from the true model, and source frequencies are then changed from lower to higher bands (as in the 'frequency-cascade scheme') to estimate model elastic parameters. Elastic parameters are inverted at each inversion step ('simultaneous mode') with a starting model of linear P- and S-wave velocity trends with depth. Elastic parameters are also derived by inversion in three other modes - using a P- and S-wave velocity basis $('V_P\;V_S\;mode')$; P-impedance and Poisson's ratio basis $('I_P\;Poisson\;mode')$; and P- and S-impedance $('I_P\;I_S\;mode')$. Density values are updated at each elastic inversion step under three assumptions in each mode. By evaluating the accuracy of the inversion for each parameter set for elastic models, it can be concluded that there is no specific difference between the inversion results for the $V_P\;V_S$ mode and the $I_P$ Poisson mode. The same conclusion is expected for the $I_P\;I_S$ mode, too. This gives us a sound basis for full wavelength elastic wavefield inversion.

Device and Circuit Level Performance Comparison of Tunnel FET Architectures and Impact of Heterogeneous Gate Dielectric

  • Narang, Rakhi;Saxena, Manoj;Gupta, R.S.;Gupta, Mridula
    • JSTS:Journal of Semiconductor Technology and Science
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    • v.13 no.3
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    • pp.224-236
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    • 2013
  • This work presents a comparative study of four Double Gate tunnel FET (DG-TFET) architectures: conventional p-i-n DG-TFET, p-n-p-n DG-TFET, a gate dielectric engineered Heterogate (HG) p-i-n DG-TFET and a new device architecture with the merits of both Hetero Gate and p-n-p-n, i.e. HG p-n-p-n DG-TFET. It has been shown that, the problem of high gate capacitance along with low ON current for a p-i-n TFET, which severely hampers the circuit performance of TFET can be overcome by using a p-n-p-n TFET with a dielectric engineered Hetero-gate architecture (i.e. HG p-n-p-n). P-n-p-n architecture improves the ON current and the heterogeneous dielectric helps in reducing the gate capacitance and suppressing the ambipolar behavior. Moreover, the HG architecture does not degrade the output characteristics, unlike the gate drain underlap architecture, and effectively reduces the gate capacitance.

Roles of i-SiC Buffer Layer in Amorphous p-SiC/i-SiC/i-Si/n-Si Thin Film Solar Cells (비정질 p-SiC/i-SiC/i-Si/n-Si 박막 태양전지에서 i-SiC 완충층의 역할)

  • Kim, Hyun-Chul;Shin, Hyuck-Jae;Lee, Jae-Shin
    • Korean Journal of Materials Research
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    • v.9 no.12
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    • pp.1155-1159
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    • 1999
  • Thin film solar cells on a glass/$SnO_2$ substrate with p-SiC/i-Si/n-Si heterojunction structures were fabricated using a plasma-enhanced chemical-vapor deposition system. The photovoltaic properties of the solar cells were examined with varying the gas phase composition, x=$CH_4/\;(SiH_4+CH_4)$, during the deposition of the p-SiC layer. In the range of x=0~0.4, the efficiency of solar cell increased because of the increased band gap of the p-SiC window layer. Further increase in the gas phase composition, however, led to a decrease in the cell efficiency probably due to in the increased composition mismatch at the p-SiC/i-Si layers. As a result, the efficiency of a glass/$SnO_2$/p-SiC/i-SiC/i-Si/n-Si/Ag thin film solar cell with $1cm^2$ area was 8.6% ($V_{oc}$=0.85V, $J_{sc}$=16.42mA/$cm^2$, FF=0.615) under 100mW/$cm^2$ light intensity.

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Exact Tests for Variance Ratios in Unbalanced Random Effect Linear Models

  • Huh, Moon-Yul;Li, Seung-Chun
    • Journal of the Korean Statistical Society
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    • v.25 no.4
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    • pp.457-469
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    • 1996
  • In this paper, we propose a method for an exact test of H : $p_i$ = $r_i$ for all i against K : $p_i$ $\neq$ $r_i$ for some i in an unbalanced random effect linear model, where $p_i$ denotes the ratio of the i-th variance component to the error variance. Then we present a method to test H : $p_i$ $\leq$ r against K : $p_i$> r for some specific i by applying orthogonal projection on the model. We also show that any test statistic that follows an F-distribution on the boundary of the hypotheses is equal to the one given here.

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Studies on the Inulin Hydrolyzing Enzyme from Aspergillu sp. (C-58) (III) - Purification of inulase (P-I) from Aspergillus sp. (C-58) - (Aspergillus sp. (C-58)균주가 생산하는 Inulin 분해효소에 관한 연구 - Aspergillus sp. C-58균주가 생산하는 inulase P-I의 정제 -)

  • Kwon, Tae-Jong;Seu, Jung-Hwu
    • Microbiology and Biotechnology Letters
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    • v.11 no.1
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    • pp.47-52
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    • 1983
  • The extracellular inulase produced by Aspergillus sp. C-58 was isolated by pH and charcoal treatment, precipitation with ammonium sulfate from the crude extract, and separated into 3 fractions (P-I, II, III) by DEAE-cellulose column chromatography in the ratio of 31.1:1.7:1 with respect to the activity. The ratio of inulase activity to sucrase activity of P-I, P-II and P-III fraction was 0.23, 0.24 and 1.1 respectively. The enzyme P-I fraction was purified 482 fold with a 22.8% yield by DEAE-Sephadex A-50, Sephadex G-75, Sephadex G-100 (1st and 2nd) column chromatography, and appeared homogeneous on polyacrylamide disc gel electrophoresis and ultracentrifugation.

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On 2-Absorbing and Weakly 2-Absorbing Primary Ideals of a Commutative Semiring

  • Soheilnia, Fatemeh
    • Kyungpook Mathematical Journal
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    • v.56 no.1
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    • pp.107-120
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    • 2016
  • Let R be a commutative semiring. The purpose of this note is to investigate the concept of 2-absorbing (resp., weakly 2-absorbing) primary ideals generalizing of 2-absorbing (resp., weakly 2-absorbing) ideals of semirings. A proper ideal I of R said to be a 2-absorbing (resp., weakly 2-absorbing) primary ideal if whenever $a,b,c{\in}R$ such that $abc{\in}I$ (resp., $0{\neq}abc{\in}I$), then either $ab{\in}I$ or $bc{\in}\sqrt{I}$ or $ac{\in}\sqrt{I}$. Moreover, when I is a Q-ideal and P is a k-ideal of R/I with $I{\subseteq}P$, it is shown that if P is a 2-absorbing (resp., weakly 2-absorbing) primary ideal of R, then P/I is a 2-absorbing (resp., weakly 2-absorbing) primary ideal of R/I and it is also proved that if I and P/I are weakly 2-absorbing primary ideals, then P is a weakly 2-absorbing primary ideal of R.