• Biomolecules & Therapeutics
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• 제2권4호
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• pp.322-325
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• 1994

To optimize the immunological efficacy of CFC-101, an outer-membrane protein vaccine purified from relatively less pathogenic 4 different Pseudomonas aeruginosa strains, we investigated to establish its dose, administration route, interval and frequency of vaccination in mice. As expected, the 4 CFC-101 producing strains were less pathogenic than the challenging organism, P. aeruginosa GN11189. CFC-101 completely protected the death caused by P. aeruginosa at above 0.05 mg/kg vaccinized by 3 times with 7-day intervals. At the optimally effective dose of 0.2 mg/kg of CFC-101, at least 3 immunizations were necessary for complete protection against P. aeruginosa-induced death. If immunized 3 times, the immunization interval could be shortened up to 2 days to acquire the best protection against P. aeruginosa. CFC-101 was effective either by intraperitoneal, subcutaneous or intramuscular but not by oral administration. The present results show that the newly developed Pseudomonas vaccine, CFC-101, is highly effective for the protection from death caused by pseudomonal infections.

• 한국안광학회지
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• 제9권2호
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• pp.353-359
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• 2004

Pseudomonas aeruginosa에 대한 soft contact lens 다목적 용액(multi purpose solution, MPS)의 항균효과의 검정을 탁도측정법에 의해 실시하였다. Muller Hinton Broth 액체배지에 pseudomonas aeruginosa를 배양한 후 5개사의 5종류의 MPS를 이용해 각각 시간별로 처리해 대조군과 비교하여 항균효과를 비교해 본 결과 한 제품을 제외하고는 거의 비슷한 효과를 나타내었다. 또한 동일한 조건하에서 MPS의 항균효과는 구성성분, pH에 따라 달라질 수 있으며 5개 회사제품 중 4개에서 pseudomonas aeruginosa에 대한 항균효과가 있는 것으로 나타났다.

• 미생물학회지
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• 제45권4호
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• pp.362-367
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• 2009

대청호의 한 지류인 소옥천으로부터 고효율 조류억제 세균을 분리하기 위하여 176균주를 분리 스크린하였으며, 이 중 AJ1이 가장 높은 조류성장억제능을 나타내었다(지름 50.0 mm 성장억제환). 조류성장억제능이 높았던 AJ1 균주 동정을 위하여 형태학적, 생리 생화학적, 16S rRNA gene sequence 분석, 지방산 분석을 수행하였으며 그 결과, Pseudomonas aeruginosa 로 판별되었다. AJ1 배양액을 원심분리한 후 상등액을 조류배양액에 첨가 시, 상등액에 의한 조류성장억제능이 나타남에 따라 세포 외 물질 분비에 의한 것을 확인할 수 있었다. 가장 높은 조류성장억 제능[60.2(${\pm}$1.3)%]은 탄소원으로 mannitol을 사용하고, 온도 $30^{\circ}C$, pH 8에서 배양할 때 보였다. 또한, AJ1 균주의 배양기간 및 투여시기에 따른 조류성장억제능 평가 결과, 조류 성장 초기 단계에 조류성장억제균을 투여하였을 때 조류성장억제능이 높게 나타났고, 균주의 배양기간에 따른 조류성장억제능은 연관성이 나타나지 않았다. 상등액 접종량에 따른 조류성장억제능은 상등액의 접종량이 높아질수록 M. aeruginosa의 제거량은 증가하였으며, 고농도(40ml/L)로 적용하였을 때 80.3(${\pm}$8.6)%의 가장 높은 M. aeruginosa 제거효율을 보여주었다. 제거 속도의 경우 상등액 접종량이 낮아질수록 M. aeruginosa 제거율이 높아지는 경향을 확인하였으며, 저농도(10 ml/L)로 적용시 $8.2{\mu}g$ chl-a/supernatant ml/day로 가장 높게 나타났다. 본 연구 결과, AJ1 균주의 현장 적용 시 M. aeruginosa의 제어에 효율적일 것으로 사료된다.

• 미생물학회지
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• 제48권3호
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• pp.200-206
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• 2012

활성슬러지로부터 특이한 조성의 polyhydroxyalkanoates (PHAs)를 생합성하는 Pseudomonas aeruginosa P-5를 분리하였다. 이 균주는 nonanoic acid나 heptanoic acid와 같은 홀수개의 탄소수를 가지는 지방산을 단일 탄소원으로 공급해주었을 경우, 3-hydroxyvalerate (3HV)와 medium-chain-length (MCL) 3-hydroxyalkanoates 단위체로 이루어진 공중합체를 생산하였다. 공중합체 내 3HV의 함량은 valeric acid와 같은 보조기질을 공급함으로써 증가시킬 수 있었으며, 2 g/L nonanoic acid와 1 g/L valeric acid로 이루어진 혼합기질로부터 3HV의 함량이 26 mol%에 달하는 공중합체를 얻을 수 있었다. 이러한 공중합체는 결정성이 매우 낮아 점착성 고분자로서의 성질을 보였다. P. aeruginosa P-5 균주는 MCL-PHA synthase 유전자(phaC1, phaC2)를 가지고 있는 반면에 SCL-PHA synthase 유전자는 결여되어 있는 것으로 나타났다. 따라서 P. aeruginosa P-5 균주의 MCL-PHA synthase는 MCL(R)-3-hydroxyacyl-CoAs 뿐만 아니라 (R)-3-hydroxyvaleryl-CoA를 기질로 인지하는 특이한 기질특이성을 갖는 것으로 사료된다.

• 생태와환경
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• 제39권2호통권116호
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• pp.209-218
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• 2006

두 종의 초식성 동물플랑크톤(Daphnia magna. Moina macrocopa)의 직접노출 밀도차(0, 4, 8개체)와 동물플랑크톤 배양여과액(ZCMF) 농도차(0, 25, 50%)에 따른, 두남조 Microcystis aeruginosa와 Planktothrix agardhii의 생체량과 세포내 microcystin(MC)을 1일 간격으로 측정하였고, 직접노출실험의 경우 동물플랑크톤 생존륜을 1일 간격으로 관찰하였다. 동물플랑크톤 직접노출 실험 결과, 두 균주에서 세포내 MC함량이 증가하였으며 (rm-ANOVA: P< 0.039), P. agardhii균주에서는 4개체 처리군(ZT1)보다 8개체 처리군(ZT2)에서 통계적으로 유의한 수준으로 높은 세포내 MC값이 관찰되었다(Tukey test. P<0.082).세포내 MC가 최고값을 보인 3일 또는 4일째에, 처리군들은 대조군에 비하여 통계적으로 유의한 차이를 보였다(One-way ANOVA, P< 0.021). ZCMF에 노출시킨 M. aeruginosa균주에서 세포내 MC함량이 통계적으로 유의한 수준으로 차이가 있었다(rm-ANOVA: P< 0.004), 실험기간동안 세포내 MC값은 M. aeruginosa균주에서 25%처리군 보다 50%처리군에서 통계적으로 유의한 수준으로 높게 나타났다(Tukey test, Plt; 0.025). 본연구결과, 세포내 MC량은 초식성 동물플랑크톤의 직접노출 밀도차와 분비화학물질(infochemical)농도차에 의존하여 증가 혹은 변화될수 있는 가능성이 있는 것으로 나타났으며, 부영양호에서 독성 남조 번성 조절시, 이러한 가능성을 고려하여 생물조절이 이루어져야 할 것으로 보인다.

• Journal of Microbiology and Biotechnology
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• 제26권10호
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• pp.1701-1707
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• 2016

Lipoxygenase (LOX) is an industrial enzyme with wide applications in food and pharmaceutical industries. The available structure information indicates that eukaryotic LOXs consist of N terminus β-barrel and C terminus catalytic domains. However, the latest crystal structure of Pseudomonas aeruginosa LOX shows it is significantly different from those of eukaryotic LOXs, including the N-terminal helix domain. In this paper, the functions of this N-terminal helix domain in the soluble expression and catalysis of P. aeruginosa LOX were analyzed. Genetic truncation of this helix domain resulted in an insoluble P. aeruginosa LOX mutant. The active C-terminal domain was obtained by dispase digestion of the P. aeruginosa LOX derivative containing the genetically introduced dispase recognition sites. This functional C-terminal domain showed raised substrate affinity but reduced catalytic activity and thermostability. Crystal structure analyses demonstrate that the broken polar contacts connecting the two domains and the exposed hydrophobic substrate binding pocket may contribute to the insoluble expression of the C terminus domain and the changes in the enzyme properties. Our data suggest that the N terminus domain of P. aeruginosa LOX is required for its soluble expression in E. coli, which is different from that of the eukaryotic LOXs. Besides this, this N-terminal domain is not necessary for catalysis but shows positive effects on the enzyme properties. The results presented here provide new and valuable information on the functions of the N terminus helix domain of P. aeruginosa LOX and further improvement of its enzyme properties by molecular modification.

• 상하수도학회지
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• 제20권5호
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• pp.755-761
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• 2006

Recently, there is growing interest in ultraviolet (UV) irradiation as a disinfection technic in drinking water production due to its effectiveness to inactivate microorganisms such as Crytosporidium parvum without forming disinfection byproducts. However, UV disinfection is known for its drawback such as photoreactivation. Despite many works concerning the photoreactivation, most of works were focused on indicator or non pathogenic microorganisms. The objective of this study is to examine the photoreactivation of Pseudomonas aeruginosa which is an opportunistic pathogen as UV radiation by LP and MP UV lamp was applied. The result showed that P. aeruginosa had high photo repair efficiency regardless of the type of UV irradiation. Both of the effective log repair values of LP and MP UV irradiation were found approximately 2.6 log. In addition, photo repaired P. aeruginosa was not significantly different in forming biofilm in comparison with non treated P. aeruginosa.

• 한국물환경학회지
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• 제33권4호
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• pp.441-448
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• 2017

Microcystis aeruginosa (M. aeruginosa) is a cyanobacterium species that can form harmful algal blooms in freshwater bodies worldwide. The use of Artemisia asiatica extracts to control M. aeruginosa inhibition will be environmentally friendly and promising. Artemisia asiatica extracts removed successfully upto 88% of M. aeruginosa pH 8 at $25^{\circ}C$ of temperature. These results was indicated that the amount of 2.24 g/L Artemisia asiatica extracts was removed 1g dryweight/L of M. aeruginosa. The kinetic data showed substrate inhibition kinetics and maximum growth rate was obtained when the M. aeruginosa was grown in medium containing 2.5 g/L of initial concentration of Artemisia asiatica extracts. In the various growth control models, Luong model showed the highest correlation coefficient of 0.9916. Therefore, the Luong model was the most suitable control model for the growth control of M. aruginosa using Artemisia asiatica extracts. In conclusion, the growth control of M. aruginosa using Artemisia asiatica extracts can be applied in the field without controlling the temperature and pH of rivers and streams, and it is possible to control the growth of M. aruginosa efficiently in a short time. The natural extract, Artemisia asiatica extracts, can be a promising inhibition due to its high efficiency and low dose requirements.

• KSBB Journal
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• 제13권5호
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• pp.511-517
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• 1998

Temperature and pH conditions were studied for an effective biosurfactant production by Pseudomonas aeruginosa YPJ-80. Efficient methods of biosurfactant separation were also investigated. pH-uncontrolled experiments at 35$^{\circ}C$ and an initial pH of 8 resulted in the best cell growth (3.6 g/L) and biosurfactant production (0.073 g biosurfactant/g cell). Biosurfactant separation was most efficient using solvent extraction with chloroform/methanol (2:1 vol%) followed by acidification using 1N HCl.

• 한국임상수의학회지
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• 제36권5호
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• pp.245-247
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• 2019

Taylorella equigenitalis (T. equigenitalis), Klebsiella pneumoniae (K. pneumoniae), and Pseudomonas aeruginosa (P. aeruginosa) are sexually transmittable bacteria known to cause venereal diseases (VD) in horses. T. equigenitalis causes contagious equine metritis (CEM), which is a considerable concern for equine breeding industry. K. pneumoniae and P. aeruginosa may cause endometritis and infertility in susceptible mares. The purpose of this study was to investigate the prevalence of these bacteria among breeding Thoroughbreds in South Korea. External genital swabs were collected from 178 breeding Thoroughbreds, including 11 stallions and 167 mares. The samples were tested using a commercial multiplex real-time PCR kit. T. equigenitalis, P. aeruginosa, and K. pneumoniae were present in 5.6%, 7.3%, and 5.6% of tested Thoroughbreds, respectively. The results highlight the need for regular testing of South Korean Thoroughbreds, particularly those used for breeding, for these bacteria. The regular pre-breeding test for these bacteria will prevent health complications for the horse and financial losses for the owner as a result of VD.