DOI QR코드

DOI QR Code

PCR-Based Determination of the Prevalence of Common Venereal Bacterial Pathogens in Breeding Thoroughbreds of South Korea

  • Lee, Sang-Kyu (College of Veterinary Medicine, Seoul National University) ;
  • Lee, Inhyung (College of Veterinary Medicine, Seoul National University)
  • Received : 2019.03.18
  • Accepted : 2019.09.03
  • Published : 2019.10.31

Abstract

Taylorella equigenitalis (T. equigenitalis), Klebsiella pneumoniae (K. pneumoniae), and Pseudomonas aeruginosa (P. aeruginosa) are sexually transmittable bacteria known to cause venereal diseases (VD) in horses. T. equigenitalis causes contagious equine metritis (CEM), which is a considerable concern for equine breeding industry. K. pneumoniae and P. aeruginosa may cause endometritis and infertility in susceptible mares. The purpose of this study was to investigate the prevalence of these bacteria among breeding Thoroughbreds in South Korea. External genital swabs were collected from 178 breeding Thoroughbreds, including 11 stallions and 167 mares. The samples were tested using a commercial multiplex real-time PCR kit. T. equigenitalis, P. aeruginosa, and K. pneumoniae were present in 5.6%, 7.3%, and 5.6% of tested Thoroughbreds, respectively. The results highlight the need for regular testing of South Korean Thoroughbreds, particularly those used for breeding, for these bacteria. The regular pre-breeding test for these bacteria will prevent health complications for the horse and financial losses for the owner as a result of VD.

Keywords

References

  1. Blanchard TL, Kenney RM, Timoney PJ. Venereal disease. Vet Clin North Am Equine Pract 1992; 8: 191-203. https://doi.org/10.1016/S0749-0739(17)30475-3
  2. Causey RC. Uterine therapy for mares with bacterial infections. In: Current Therapy in Equine Reproduction. St. Louis: Saunders Elsevier. 2007: 105-107.
  3. Jeong HY, Lee SK, Park JY, Kim HJ, Yang SJ, Lee SK, Ko JA, Yang SK, Park C, Kim SH, Kang HE, Choi JG. Status of Taylorella equigenitalis Infection in Thoroughbred Horses in the Republic of Korea and the Molecular Characterization of the Korean Taylorella equigenitalis Isolates. J Equi Vet Sci 2018; 69: 102-107. https://doi.org/10.1016/j.jevs.2018.06.012
  4. Metcalf ES. Venereal disease. In: Equine Reproduction, 2nd edn. Chichester: Wiley. 2011.
  5. Ministry of Agriculture, Food and Rural Affairs website. Act on the prevention of contagious animal diseasess. Available at: http://www.mafra.go.kr. Accessed Mar 05, 2019.
  6. Moore JE, Buckley TC, Millar BC, Gibson P, Cannon G, Egan C, Cosgrove H, Stanbridge S, Anzai T, Matsuda M, Murphy PG. Molecular surveillance of the incidence of Taylorella equigenitalis and Pseudomonas aeruginosa from horses in Ireland by sequence-specific PCR. Equine Vet J 2001; 33: 319-22. https://doi.org/10.2746/042516401776249750
  7. Morel MCGD. Infertility. In: Equine Reproductive Physiology, Breeding and Stud Management. Ipswich: Farming Press Books 1993: 386-392.
  8. Pasolini MP, Prete CD, Fabbri S, Auletta L. Endometritis and infertility in the mare - the challenge in equine Breeding Industry-a review. In: Genital Infections and Infertility. London: IntechOpen. 2016: 290.
  9. Ricketts S, Troedsson MHT. Fertility expectations and management for optimal fertility. In: Current Therapy in Equine Reproduction. St. Louis: Saunders Elsevier. 2007: 54-55.
  10. Samper JC, Tibary A. Disease transmission in horses. Theriogenology 2006; 66: 551-559. https://doi.org/10.1016/j.theriogenology.2006.04.019
  11. Satue K, Gardon JC. Infection and Infertility in Mares. In: Genital Infections and Infertility. London: IntechOpen. 2016: 257-283.
  12. Snider TA. Reproductive Disorders in Horses. Vet Clin North Am Equine Pract 2015; 31: 389-405. https://doi.org/10.1016/j.cveq.2015.04.011
  13. Tibary A, Pearson LK, Fite CL. Reproductive tract infections. In: Equine infectious diseases, 2nd edn. St. Louis: Saunders. 2013: 104-106.
  14. Troedsson MHT. Endometritis. In: Equine Reproduction, 2nd edn. Chichester: Wiley. 2011: 2609-2613.
  15. Horseracing Betting Betting Levyboard website. Codes of Practice 2019. Availabe at: https://codes.hblb.org.uk. Accessed Mar 05, 2019.
  16. Wilson D. Endometritis, bacterial and fungal. In: Clinical veterinary advisor : the horse. St. Louis: Saunders. 2010: 166-168.