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http://dx.doi.org/10.7845/kjm.2012.031

Biosynthesis of Copolyesters Consisting of 3-Hydroxyvalerate and Medium-chain-length 3-hydroxyalkanoates by the Pseudomonas aeruginosa P-5 Strain  

Woo, Sang-Hee (Department of Microbiology and Molecular Biology, Chungnam National University)
Kim, Jae-Hee (Department of Microbiology and Molecular Biology, Chungnam National University)
Ni, Yu-Yang (Department of Microbiology and Molecular Biology, Chungnam National University)
Rhee, Young-Ha (Department of Microbiology and Molecular Biology, Chungnam National University)
Publication Information
Korean Journal of Microbiology / v.48, no.3, 2012 , pp. 200-206 More about this Journal
Abstract
A bacterial strain capable of synthesizing polyhydroxyalkanoates (PHAs) with an unusual pattern of monomer units was isolated from activated sludge using the enrichment culture technique. The organism, identified as Pseudomonas aeruginosa P-5, produced polyesters consisting of 3-hydroxyvalerate and medium-chain-length (MCL) 3-hydroxyalkanoate monomer units when $C_{-odd}$ alkanoic acids such as nonanoic acid and heptanoic acid were fed as the sole carbon source. Solvent fractionation experiments using chloroform and hexane revealed that the 3-hydroxyalkanoate monomer units in these polyesters were copolymerized. The molar concentration of 3-hydroxyvalerate in the polyesters produced were significantly elevated up to 26 mol% by adding 1.0 g/L valeric acid as the cosubstrate. These copolyesters were sticky with low degrees of crystallinity. The PHA synthase genes were cloned, and the deduced amino acid sequences were determined. P. aeruginosa P-5 possessed genes encoding MCL-PHA synthases (PhaC1 and PhaC2) but lacked the short-chain-length PHA synthase gene, suggesting that the MCL-PHA synthases from P. aeruginosa P-5 are uniquely active for polymerizing (R)-3-hydroxyvaleryl-CoA as well as MCL (R)-3-hydroxyacyl-CoAs.
Keywords
Pseudomonas aeruginosa; 3-hydroxyvalerate; MCL-PHA synthase; polyhydroxyalkanoate;
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