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Antimicrobial Activity of Ethanol Extract from Sargassum thunbergii (지충이(Sargassum thunbergii) 에탄올 추출물의 항균활성)

  • Lee, So-Young;Song, Eu-Jin;Kim, Koth-Bong-Woo-Ri;Yoon, So-Young;Kim, Seo-Jin;Lee, So-Jeong;Hong, Yong-Ki;Lim, Sung-Mee;Ahn, Dong-Hyun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.38 no.4
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    • pp.502-508
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    • 2009
  • Antimicrobial activity of Sargassum thunbergii was determined by paper disc assay and minimum concentration inhibitor (MIC) test. A water extract of S. thunbergii did not show the antimicrobial activity, but an ethanol extract of S. thunbergii (SHE) inhibited Serratia liquefaciens, Salmonella Typhimurium, Pseudomonas aerogenosa and all of the tested gram-positive bacteria at 4 mg/mL. Especially, Bacillus subtilis, Clostridium perfringens and Listeria monocytogenes were susceptible to SHE. As the results of MIC test, SHE inhibited the growth of B. subtilis, Staphylococcus aureus and Listeria monocytogenes at concentration of $0.1{\sim}0.3%$, and inhibited C. perfringens at 0.01%. In the thermal and pH stability test for SHE, antibacterial activities of SHE were maintained when the SHE was treated at $121^{\circ}C$ for 15 minutes or under pH $2{\sim}8$. SHE was partitioned in the order of n-hexane, chloroform, ethyl acetate and butanol. As the results of the MIC test for each obtained fraction, no fraction exhibited higher antibacterial activity than that of the crude SHE. However, a mixture of chloroform, ethylacetate and ethanol fractions showed higher antibacterial activity than SHE.

The Effect of Carbon Dioxide Leaked from Geological Storage Site on Soil Fertility: A Study on Artificial Leakage (지중 저장지로부터 누출된 이산화탄소가 토양 비옥도에 미치는 영향: 인위 누출 연구)

  • Baek, Seung Han;Lee, Sang-Woo;Lee, Woo-Chun;Yun, Seong-Taek;Kim, Soon-Oh
    • Economic and Environmental Geology
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    • v.54 no.4
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    • pp.409-425
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    • 2021
  • Carbon dioxide has been known to be a typical greenhouse gas causing global warming, and a number of efforts have been proposed to reduce its concentration in the atmosphere. Among them, carbon dioxide capture and storage (CCS) has been taken into great account to accomplish the target reduction of carbon dioxide. In order to commercialize the CCS, its safety should be secured. In particular, if the stored carbon dioxide is leaked in the arable land, serious problems could come up in terms of crop growth. This study was conducted to investigate the effect of carbon dioxide leaked from storage sites on soil fertility. The leakage of carbon dioxide was simulated using the facility of its artificial injection into soils in the laboratory. Several soil chemical properties, such as pH, cation exchange capacity, electrical conductivity, the concentrations of exchangeable cations, nitrogen (N) (total-N, nitrate-N, and ammonia-N), phosphorus (P) (total-P and available-P), sulfur (S) (total-S and available-S), available-boron (B), and the contents of soil organic matter, were monitored as indicators of soil fertility during the period of artificial injection of carbon dioxide. Two kinds of soils, such as non-cultivated and cultivated soils, were compared in the artificial injection tests, and the latter included maize- and soybean-cultivated soils. The non-cultivated soil (NCS) was sandy soil of 42.6% porosity, the maize-cultivated soil (MCS) and soybean-cultivated soil (SCS) were loamy sand having 46.8% and 48.0% of porosities, respectively. The artificial injection facility had six columns: one was for the control without carbon dioxide injection, and the other five columns were used for the injections tests. Total injection periods for NCS and MCS/SCS were 60 and 70 days, respectively, and artificial rainfall events were simulated using one pore volume after the 12-day injection for the NCS and the 14-day injection for the MCS/SCS. After each rainfall event, the soil fertility indicators were measured for soil and leachate solution, and they were compared before and after the injection of carbon dioxide. The results indicate that the residual concentrations of exchangeable cations, total-N, total-P, the content of soil organic matter, and electrical conductivity were not likely to be affected by the injection of carbon dioxide. However, the residual concentrations of nitrate-N, ammonia-N, available-P, available-S, and available-B tended to decrease after the carbon dioxide injection, indicating that soil fertility might be reduced. Meanwhile, soil pH did not seem to be influenced due to the buffering capacity of soils, but it is speculated that a long-term leakage of carbon dioxide might bring about soil acidification.

Novel quantitative trait loci for the strong-culm and high-yield related traits in rice detected from the F2 population between the super thick-culm and super grain-bearing line 'LTAT-29' and the high-yielding variety 'Takanari'

  • Nomura, Tomohiro;Yamamoto, Toshio;Ueda, Tadamasa;Yonemaru, Junichi;Abe, Akira;Adachi, Shunsuke;Hirasawa, Tadashi;Ookawa, Taiichiro
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.95-95
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    • 2017
  • Lodging is a serious issue in rice production, because it drastically decreases the biomass production and grain yield. Since the Green Revolution, the lodging resistance has been increased by lowering the moment of above-ground parts due to the short culm by the semi-dwarf gene sd1. However, it has been pointed out that sd1 alone has suppressive effects for biomass production and yield. To increase rice yield, the long-culm and large panicle type varieties with a superior lodging resistance need to be developed. To improve the lodging resistance and yield of these type varieties, it would be effective to identify novel alleles for these traits underlying natural variations in rice and to pyramid these alleles to a single rice variety. In order to perform this strategy, we have developed new rice lines derived from crosses among varieties with superior alleles. At first, TULT-gh-5-5 was selected from a cross between strong culm and high biomass variety Leaf Star and high-yielding variety Takanari, and TUAT-32HB was selected from a cross between high-yielding variety Akenohoshi and Takanari. Then, we developed the super thick-culm and super grain-bearing line, LTAT-29 derived from a cross between TULT-gh-5-5 and TUAT-32HB. In the current study, to identify the QTLs and genes relating to the strong culm and the high yield of LTAT-29, we performed QTL analysis using SNPs markers with $F_2$ population derived from a cross between LTAT-29 and Takanari. LTAT-29 has never lodged throughout the growth period despite it had long culms and heavy panicles. LTAT-29 had a larger outer diameter of the culm and twice the size of the section modulus than Takanari. As a result, the bending moment at breaking of LTAT-29 was significantly larger than that of Takanari. Brown rice yield of LTAT-29 was $9.2t\;ha^{-1}$ about 10% higher than that of Takanari due to the larger number of spikelets per panicle. LTAT-29 had a greater number of secondary branches per panicle. In the $F_2$ population between LTAT-29 and Takanari, we found continuous frequency distributions in the section modulus and the spikelet number per panicle. Two QTLs increased the section modulus by the alleles of LTAT-29 were detected on Chr.1L and Chr.2L. One QTL increased the spikelet number per panicle of Takanari by the allele of LTAT-29 was detected on Chr.1L, and two QTLs increased the number of secondary branches per panicle by the alleles of LTAT-29 were detected on Chr.1L and Chr.4L. It was found that the alleles of these QTLs were the japonica type originated from Leaf Star or Akenohoshi. The novel QTLs for the traits related to super thick-culm and super grain-bearing and their combinations could be utilized for improving the lodging resistance and yield in rice varieties.

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Agronomic Characteristics of Adzuki bean (Vigna angularis W.F. Wight) Germplasm in Korea (팥 유전자원의 작물학적 형질 특성)

  • Yoon, Seong-Tak;Qin, Yufeng;Kim, Tae-Ho;Choi, Seong-Hoon;Nam, Jung-Chang;Lee, Jun-Soo
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.57 no.1
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    • pp.7-15
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    • 2012
  • Agronomic characteristics of 150 adzuki bean ($Vigna$ $angularis$ W.F. Wight) germplasm collected in Korea were investigated in order to establish basic data for the improvement of crop breeding. Growth types were classified as 3 types of indeterminate type, determinate type, and semideterminate type, of which indeterminate type was the highest percentage of 96.6%. Seed color were classified as 8 colors of which dark brown took 66.5% in 150 adzuki bean germplasm. Days from seeding to maturity showed the range from 89 to 142 days with 115 days in the average and the highest frequency proportion was 37.2% of the group from 131 to 140 days, and the next was 27.3% of the group from 121 to 130 days among 150 germplasm. Pod length showed the range from 5.9 to 10.1 cm with 7.8 cm in the average and the highest frequency distribution of it was the group from 7.1 to 8.0 cm with the proportion of 45.1% and the next was the group from 8.1 to 9.0 cm with the proportion of 32.2%. Number of pods per plant showed the range from 5.0 to 132.5 pods with 28.5 pods in the average and the highest frequency distribution of it was the group from 11.0 to 20.0 pods with the proportion of 30.8% and the next was the group from 21.0 to 30.0 pods with the proportion of 28.6%. Number of seeds per pod showed the range from 5.3 to 11.3 seeds with 8.2 seeds in the average and the highest frequency proportion of it was the group from 7.1 to 8.0 seeds, which occupied 32.2% among 150 germplasm. 89.3% of 150 germplasm showed the resistance to shattering of grains at harvest period. 100 grain weight showed the range from 5.7 to 23.0 g with 12.9 g in the average and the highest frequency proportion of it was the group from 9.01 to 13.0 g, which occupied 43.3% and the next was the group from 13.01 to 17.00 g with the proportion of 28.5% of 150 germplasm.

Development and Application of the High Speed Weigh-in-motion for Overweight Enforcement (고속축하중측정시스템 개발과 과적단속시스템 적용방안 연구)

  • Kwon, Soon-Min;Suh, Young-Chan
    • International Journal of Highway Engineering
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    • v.11 no.4
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    • pp.69-78
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    • 2009
  • Korea has achieved significant economic growth with building the Gyeongbu Expressway. As the number of new road construction projects has decreased, it becomes more important to maintain optimal status of the current road networks. One of the best ways to accomplish it is weight enforcement as active control measure of traffic load. This study is to develop High-speed Weigh-in-motion System in order to enhance efficiency of weight enforcement, and to analyze patterns of overloaded trucks on highways through the system. Furthermore, it is to review possibilities of developing overweight control system with application of the HS-WIM system. The HS-WIM system developed by this study consists of two sets of an axle load sensor, a loop sensor and a wandering sensor on each lane. A wandering sensor detects whether a travelling vehicle is off the lane or not with the function of checking the location of tire imprint. The sensor of the WIM system has better function of classifying types of vehicles than other existing systems by detecting wheel distance and tire type such as single or dual tire. As a result, its measurement errors regarding 12 types of vehicle classification are very low, which is an advantage of the sensor. The verification tests of the system under all conditions showed that the mean measurement errors of axle weight and gross axle weight were within 15 percent and 7 percent respectively. According to the WIM rate standard of the COST-323, the WIM system of this study is ranked at B(10). It means the system is appropriate for the purpose of design, maintenance and valuation of road infrastructure. The WIM system in testing a 5-axle cargo truck, the most frequently overloaded vehicle among 12 types of vehicles, is ranked at A(5) which means the system is available to control overloaded vehicles. In this case, the measurement errors of axle load and gross axle load were within 8 percent and 5 percent respectively. Weight analysis of all types of vehicles on highways showed that the most frequently overloaded vehicles were type 5, 6, 7 and 12 among 12 vehicle types. As a result, it is necessary to use more effective overweight enforcement system for vehicles which are seriously overloaded due to their lift axles. Traffic volume data depending upon vehicle types is basic information for road design and construction, maintenance, analysis of traffic flow, road policies as well as research.

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Adjustment of Nitrogen by the Absorbing Patterns of Nutrients of Some Crops and N - Leaching in the Soil (시비보정(施肥補正)을 위(爲)한 작물(作物)의 양분(養分) 흡수(吸收) 양상(樣相)과 토양중(土壤中) 비료성분(肥料成分) 용탈(溶脫)에 관(關)한 연구(硏究))

  • Kim, Moon-Kyu;Chang, Ki-Woon;Woo, In-Shik;Ham, Suon-Kyu;Nam, Yun-Kyu
    • Korean Journal of Soil Science and Fertilizer
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    • v.22 no.4
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    • pp.307-314
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    • 1989
  • Barley, garlic, and soybean were cultivated in the silt loam, sandy loam, and loam, respectively, and the absorbing patterns of N, P, and K during growing of the crops were determined. While, the leaching of N, P, and K applied in the above soils in four levels of topsoil depths and in four leachate levels of excess precipitation was measured through the column. The depths of the soil were devised to 20, 35, 50, and 65cm, and the levels of excess water were classified to each leachate of 20, 40, 60, and 80mm precipitation, and nitrogen, phosphorous, and potassium in each fraction of the leachate were analyzed. By the analyses of the chemical components during growth of the crops, their absorbing patterns of N, P, and K were investigated. The order of N-leachings in the soils was sandy loam > loam > silt loam, and the leaching of $K_2O$ was very similar to N. The leaching of $P_2O_5$ was slight in all kinds of the soils. By the combination of the absorbing patterns of the crops in three kinds of soils and the leaching of the nitrogen in four levels of soil depth and four levels of excess precipitation, the method to replace the nitrogen lost by leaching was presented.

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Evaluation of Crop Characteristics of Sorghum (Sorghum bicolor L.) Germplasm for the Selection of Excellent Resources (우수자원 선발을 위한 수수(Sorghum bicolor L.) 유전자원의 특성평가)

  • Yoon, Seong-Tak;Jeong, In-Ho;Han, Tae-Kyu;Kim, Young-Jung;Yu, Je-Bin;Yang, Gyeong;Ye, Min-Hee;Baek, Seung-Woo;Kim, Kun-Woo
    • Korean Journal of Plant Resources
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    • v.29 no.4
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    • pp.479-494
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    • 2016
  • The aim of this study is to select the superior resources of high yield, high content of functional material optimal to mechanical harvesting by the evaluation of crop growth and yield characteristics in sorghum germplasm. One hundred accessions of sorghum germplasm were used in this experiment. Days from seeding to heading date showed the range from 68 to 94 days with the highest frequency proportion was the group from 80 to 85 days, which occupied 34% (34 plant resources) of 100 germplasm. Ear types of 100 sorghum germplasm could be classified as 7 types of broom-tillering, half broom-tillering, extreme open-loose type, open-loose type, intermediate type, compact type, extreme-compact type of which intermediate type was the highest ratio of 28% (28 plant resources) of 100 germplasm. Yield showed the range from 106 to 365 ㎏/10a with the highest frequency proportion of it was the group from 150 to 200 ㎏/10a, which occupied 44% (44 plant resources) of 100 germplasm. Among 100 sorghum germplasm, 18 ideal resources of high yield and short plant height appropriate for mechanical harvesting were selected. In order to evaluate high content of functional substance, selected 18 resources were analyzed for total polyphenol content, DPPH radical scavenging activity and total anthocyanin content. Finally, we selected 5 resources of short plant height, high yield, high content of total polyphenol and high DPPH radical scavenging activity among 18 genetic resources.

The Influence of University Entrepreneurship Education's Creativity Capacity to Entrepreneurship Willingness: The Moderating Role of Social Support (창의성 역량 교육이 창업의지에 미치는 영향에 관한 연구: 사회적 지지의 조절효과를 중심으로)

  • Ahn, Tae Uk;Park, Jae Hwan;Lee, Il han
    • 한국벤처창업학회:학술대회논문집
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    • 2017.08a
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    • pp.1-13
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    • 2017
  • In the era of the 4th Industrial Revolution, as the importance of creative talent became more and more important, we recognized the urgent need for start-ups to innovate new growth industries and creative talents to lead these eras.And the importance of entrepreneurship education emphasized in entrepreneurship education and the necessity of creativity competency education.However, there is a lack of research on how creativity competency education affects entrepreneurship intention for college students. Especially, research on the moderating effect of social support on the effect of entrepreneurship through education is very insufficient. The purpose of this study is to examine the effects of creativity competence education on the will of entrepreneurship and empowerment of social support. For this purpose, 393 college students were used for empirical analysis for 1 month from August 2016. As a result of this study, the ability of communicative communication and creative problem solving had a positive effect on self - efficacy. On the other hand, innovative work behavior abilities did not directly affect self-efficacy. In addition, creative problem solving ability and innovative work behavior ability had a positive effect on the will to start up. Collaborative communication skills, however, did not directly affect the willingness to start a business. Self - efficacy has a positive effect on the will of founding. Finally, the moderating effect of social support between self-efficacy and willingness to work was not directly affected. The implication of this study is that the effect of the creativity competency education on college students has a positive effect on the self - efficacy of self and the positive influence on the will to start up. The results of this study are as follows. First, it is found that the influence of social support (parental support) is not positively influenced by the control effect of the parents. In the case of college students, As well as the need to do so. Therefore, in order to further increase the willingness of young people to establish a university in the university, it is necessary to provide education and programs not only for direct education for college students, but also for improving the understanding of the talents and entrepreneurship required by the age of their parents.

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Screening of Korean Medicinal Herbs for Hormonal Activities using Recombinant Yeast Assay and MCF-7 Human Breast Cancer Cells (재조합효모와 MCF -7 사람유방암세포주를 이용한 한국산 약용식물의 호르몬 활성 스크리닝)

  • Yang Se-Ran;Hong Hee-Do;Cho Sung-Dae;Ahn Nam-Shik;Jung Ji-Won;Park Joon-Suk;Jo Eun-Hye;Hwang Jae-Woong;Sun bo;Park Jung-Ran;Lee Seong-Hun;Jung Ji-Youn;Choi Changsun
    • Journal of Food Hygiene and Safety
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    • v.20 no.1
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    • pp.1-6
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    • 2005
  • To investigate whether there are estrogenic and anti-estrogenic activities in various medicinal herbs and discover prominent chemo-preventive agents, we screened and compared the ethanol extracts of 9 plants through the recombinant yeast assay and MCF-7 human breast cancer cell assay, In recombinant yeast assay, seven medicinal herbs showed estrogenicity, and four extracts showed androgenecity. In MCF-7 proliferation assay, the growth of MCF-7 cells was inhibited by eight extracts before and even after co-treatment with bisphenol A. It is interesting that the extracts of Glycyrrhiza uralensis, Cassia tora, Syringa velutina, Zingiber officinale, Malva verticillata, and Panax ginseng C.A. Meyer exhibited inhibitory effects as phytoestrogens in estrogen-responsive human breast cancer cells. This study suggests that some Korean medicinal herbs might be considered as phytoestrogens and be useful to further analyze those plants which contain the estrogenic effect in order to identify the active principles.

Shalf Life Enhancement of Minimally Processed Fruits and Vegetables

  • Kim, Dong-Man
    • Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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    • 1993.12a
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    • pp.6-9
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    • 1993
  • According to changes in population, economic conditions, life-stile and eating habits, the frui ts and vegetables market wi 11 be shi fted from processed (i. e. , canned) to fresh. Undressed fresh produce, consisting of washed, disinfected and peeled fruits and vegetables that either sliced or grated, are currently increased in demand by retail and institutional market which use them as salad components or in ready-to use foods, Main attributes of minimally processed fruits and vegetables are convenience and fresh-like quality. Minimally processed Products readily deteriorate in quality, especially color and texture, as a result of endogeneous enzyme enhanced respiration and microorganisms which lead to reduced shelf Iife. According to changes in population, economic conditions, life-stile and eating habits, the frui ts and vegetables market wi 11 be shi fted from processed (i. e. , canned) to fresh. Undressed fresh produce, consisting of washed, disinfected and peeled fruits and vegetables that either sliced or grated, are currently increased in demand by retail and institutional market which use them as salad components or in ready-to use foods, Main attributes of minimally processed fruits and vegetables are convenience and fresh-like quality. Minimally processed Products readily deteriorate in quality, especially color and texture, as a result of endogeneous enzyme enhanced respiration and microorganisms which lead to reduced shelf Iife. Thus. to prevent these undesirable changes , val'ious techniques such as controlled atmosphere (CA) storage, modified atmosphere OIA) storage, including vacuum packaging have been receiving considerable attention, Although milch research has been done to find optimal conditions for whole intact frui ts and vegetables, only limi ted information is avai lable on fresh cut. and other minimally processed products. 81 iced frui ts exhibi t increas~d ethylene production and respiration compal'ed to whole f, 'uits during distribution in response to tissue damage. As a result, accelerated senescence and enzymatic browning OCCUI', Recent l'esearch on minimally processed fl'uits and vegetables has mainly focused on methods to inhibit browning, due to ban on use of sulfur dioxide, In order to retard or prevent these physiological changes, val'ious al ternatives, reducing agents. acidulants, chelating agents and inol'ganic sal ts have been evaluated for use on fresh cut fl'ui ts. Al though some agents were effective replacement for sulfur dioxide. consum$\textregistered$I'S demandless use of chemical on such products. Shel~ life of minimally processed products has been extended by inhibition of metabolic reactions associated with loss of quality and by inhibition of aerobic spoilage caused by wide variety of microorganisms. Appl ication of ~I.-\ packaging, including vacuum packaging, retards the rate of respiration, prevents growth of aerobic spoilage organisms, inhibits oxidation and color deterioration. Tissue softening is another major problem in minimally processed products because enzymes re 1 a ted to ce 11 wa 11 degrada t i on are not inactivated. Various treatments have been investigated for retardation of the softening of sliced products. Some studies have concentrated on the application of an active packaging system with ~I, l. packaging and calcium infi 1 tration as possible measures to retain firmness of processed products. In my opinion, one important step for production of minimally processed frui ts wi th favorabl e color of cut surface and firm texture is the selection of better cultivar. As the view, changing tendency of fresh color by apple cultivars and relationship between the tendency and PPO activity will be discussed in the seminar. In addition to the topic, research result on quality enhancement of fresh apple slices by heat shock treatment will be introduced.

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