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Screening of Korean Medicinal Herbs for Hormonal Activities using Recombinant Yeast Assay and MCF-7 Human Breast Cancer Cells  

Yang Se-Ran (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University)
Hong Hee-Do (Korea Food Research institute, Sungnam city)
Cho Sung-Dae (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University)
Ahn Nam-Shik (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University)
Jung Ji-Won (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University)
Park Joon-Suk (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University)
Jo Eun-Hye (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University)
Hwang Jae-Woong (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University)
Sun bo (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University)
Park Jung-Ran (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University)
Lee Seong-Hun (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University)
Jung Ji-Youn (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University)
Choi Changsun (Department of Veterinary Pathology, College of Veterinary Medicine, Ohio State University, USA)
Publication Information
Journal of Food Hygiene and Safety / v.20, no.1, 2005 , pp. 1-6 More about this Journal
Abstract
To investigate whether there are estrogenic and anti-estrogenic activities in various medicinal herbs and discover prominent chemo-preventive agents, we screened and compared the ethanol extracts of 9 plants through the recombinant yeast assay and MCF-7 human breast cancer cell assay, In recombinant yeast assay, seven medicinal herbs showed estrogenicity, and four extracts showed androgenecity. In MCF-7 proliferation assay, the growth of MCF-7 cells was inhibited by eight extracts before and even after co-treatment with bisphenol A. It is interesting that the extracts of Glycyrrhiza uralensis, Cassia tora, Syringa velutina, Zingiber officinale, Malva verticillata, and Panax ginseng C.A. Meyer exhibited inhibitory effects as phytoestrogens in estrogen-responsive human breast cancer cells. This study suggests that some Korean medicinal herbs might be considered as phytoestrogens and be useful to further analyze those plants which contain the estrogenic effect in order to identify the active principles.
Keywords
Breast cancer; MCF-7 human breast cancer cell; Phytoestrogen; Recombinant yeast assay; Screening;
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1 Messina, M. & Barnes, S. The role of soy products in reducing risk of cancer. J Natl Cancer Inst 83, 541-6 (1991)   DOI   PUBMED
2 Krishnan, A. V., Stathis, P., Permuth, S. F., Tokes, L. & Feldman, D. Bisphenol-A: an estrogenic substance is released from polycarbonate flasks during autoclaving. Endocrinology 132, 2279-86 (1993)   DOI   ScienceOn
3 Breinholt, V. & Larsen, J. C. Detection of weak estrogenic flavonoids using a recombinant yeast strain and a modified MCF7 cell proliferation assay. Chem Res Toxicol 11, 622-9 (1998)   DOI   ScienceOn
4 Rivas, A. et al. Estrogenic effect of a series of bisphenol analogues on gene and protein expression in MCF-7 breast cancer cells. J Steroid Biochem Mol Biol 82, 45-53 (2002)   DOI   ScienceOn
5 Meerts, I. A. et al. In vitro estrogenicity of polybrominated diphenyl ethers, hydroxylated PDBEs, and polybrominated bisphenol A compounds. Environ Health Perspect 109, 399-407 (2001)   DOI   ScienceOn
6 Soto, A. M., Justicia, H., Wray, J. W. & Sonnenschein, C. p-Nonyl-phenol: an estrogenic xenobiotic released from 'modified' polystyrene. Environ Health Perspect 92, 167-73 (1991)   DOI   PUBMED
7 Soto, A. M. et al. The E-SCREEN assay as a tool to identify estrogens: an update on estrogenic environmental pollutants. Environ Health Perspect 103 Suppl 7, 113-22(1995)
8 Nikula, H., Talonpoika, T., Kaleva, M. & Toppari, J. Inhibition of hCG-stimulated steroidogenesis in cultured mouse Leydig tumor cells by bisphenol A and octylphenols. Toxicol Appl Pharmacol 157, 166-73 (1999)   DOI   ScienceOn
9 Stroheker, T., Picard, K., Lhuguenot, J. C., Canivenc-Lavier, M. C. & Chagnon, M. C. Steroid activities comparison of natural and food wrap compounds in human breast cancer cell lines. Food Chem Toxicol 42, 887-97(2004)   DOI   ScienceOn
10 Lephart, E. D., Thompson, J. M., Setchell, K. D., Adlercreutz, H. & Weber, K. S. Phytoestrogens decrease brain calcium-binding proteins but do not alter hypothalamic androgen metabolizing enzymes in adult male rats. Brain Res 859, 123-31 (2000)   DOI   ScienceOn
11 Soto, A. M. & Sonnenschein, C. The role of estrogens on the proliferation of human breast tumor cells (MCF-7). J Steroid Biochem 23, 87-94 (1985)   DOI   ScienceOn
12 Hanna, K., O'Neill, S., Patterson, C. & Lyons-Wall, P. Intake of phytoestrogen-rich foods and associated lifestyle and sociodemographic characteristics in Australian women. Asia Pac J Clin Nutr 13, S73 (2004)
13 Hayakawa, J., Noda, N., Yamada, S. & Uno, K. [Studies on physical and chemical quality evaluation of crude drug preparations. I. Analysis of Pueraria Radix and species Puerariae]. Yakugaku Zasshi 104, 50-6 (1984)
14 Chen, A. C. & Donovan, S. M. Genistein at a concentration present in soy infant formula inhibits Caco-2BBe cell proliferation by causing G2/M cell cycle arrest. J Nutr 134, 1303-8 (2004)
15 Murkies, A. L., Wilcox, G. & Davis, S. R. Clinical review 92: Phytoestrogens. J Clin Endocrinol Metab 83, 297-303 (1998)   DOI   ScienceOn
16 Facemire, C. F., Gross, T. S. & Guillette, L. J., Jr. Reproductive impairment in the Florida panther: nature or nurture? Environ Health Perspect 103 Suppl 4, 79-86 (1995)
17 Jo, E. H. et al. Modulations of the Bcl-2/Bax family were involved in the chemopreventive effects of licorice root (Glycyrrhiza uralensis Fisch) in MCF-7 human breast cancer cell. J Agric Food Chem 52, 1715-9 (2004)   DOI   ScienceOn
18 Gutendorf, B. & Westendorf, J. Comparison of an array of in vitro assays for the assessment of the estrogenic potential of natural and synthetic estrogens, phytoestrogens and xenoestrogens. Toxicology 166, 79-89 (2001)   DOI   PUBMED   ScienceOn
19 Ekbom, A. & Akre, O. Increasing incidence of testicular cancer--birth cohort effects. Apmis 106, 225-9; discussion 229-31 (1998)   DOI   ScienceOn
20 Upmeier, A., Degen, G. H., Diel, P., Michna, H. & Bolt, H. M. Toxicokinetics of bisphenol A in female DA/Han rats after a single i.v. and oral administration. Arch Toxicol 74, 431-6 (2000)   DOI   ScienceOn
21 Lee, Y. S. et al. Requirement of metabolic activation for estrogenic activity of Pueraria mirifica. J Vet Sci 3, 273-7 (2002)
22 Kang, K. S., Che, J. H. & Lee, Y. S. Lack of adverse effects in the F1 offspring maternally exposed to genistein at human intake dose level. Food Chem Toxicol 40, 43-51 (2002)   DOI   ScienceOn
23 Ashby, J. & Tinwell, H. Uterotrophic activity of bisphenol A in the immature rat. Environ Health Perspect 106, 719-20 (1998)   DOI   ScienceOn
24 Setchell, K. D. Phytoestrogens: the biochemistry, physiology, and implications for human health of soy isoflavones. Am J Clin Nutr 68, 1333S-1346S (1998)
25 Knight, D. C. & Eden, J. A. A review of the clinical effects of phytoestrogens. Obstet Gynecol 87, 897-904 (1996)
26 Ramanathan, L. & Gray, W. G. Identification and characterization of a phytoestrogen-specific gene from the MCF-7 human breast cancer cell. Toxicol Appl Pharmacol 191, 107-17 (2003)   DOI   ScienceOn
27 Legler, J. et al. Development of a stably transfected estrogen receptor-mediated luciferase reporter gene assay in the human T47D breast cancer cell line. Toxicol Sci 48, 55-66 (1999)   DOI   ScienceOn
28 Staples, C. A., Dorn, P. B., Klecka, G. M., O'Block, S. T. & Harris, L. R. A review of the environmental fate, effects, and exposures of bisphenol A. Chemosphere 36, 2149-73 (1998)   DOI   ScienceOn