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http://dx.doi.org/10.3746/jkfn.2009.38.4.502

Antimicrobial Activity of Ethanol Extract from Sargassum thunbergii  

Lee, So-Young (Dept. of Food Science & Technology/ Institute of Food Science, Pukyong National University)
Song, Eu-Jin (Dept. of Food Science & Technology/ Institute of Food Science, Pukyong National University)
Kim, Koth-Bong-Woo-Ri (Dept. of Food Science & Technology/ Institute of Food Science, Pukyong National University)
Yoon, So-Young (Dept. of Food Science & Technology/ Institute of Food Science, Pukyong National University)
Kim, Seo-Jin (Dept. of Food Science & Technology/ Institute of Food Science, Pukyong National University)
Lee, So-Jeong (Dept. of Food Science & Technology/ Institute of Food Science, Pukyong National University)
Hong, Yong-Ki (Dept. of Biotechnology, Pukyong National University)
Lim, Sung-Mee (Dept. of Food Science & Technology, Tongmyong University)
Ahn, Dong-Hyun (Dept. of Food Science & Technology/ Institute of Food Science, Pukyong National University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.38, no.4, 2009 , pp. 502-508 More about this Journal
Abstract
Antimicrobial activity of Sargassum thunbergii was determined by paper disc assay and minimum concentration inhibitor (MIC) test. A water extract of S. thunbergii did not show the antimicrobial activity, but an ethanol extract of S. thunbergii (SHE) inhibited Serratia liquefaciens, Salmonella Typhimurium, Pseudomonas aerogenosa and all of the tested gram-positive bacteria at 4 mg/mL. Especially, Bacillus subtilis, Clostridium perfringens and Listeria monocytogenes were susceptible to SHE. As the results of MIC test, SHE inhibited the growth of B. subtilis, Staphylococcus aureus and Listeria monocytogenes at concentration of $0.1{\sim}0.3%$, and inhibited C. perfringens at 0.01%. In the thermal and pH stability test for SHE, antibacterial activities of SHE were maintained when the SHE was treated at $121^{\circ}C$ for 15 minutes or under pH $2{\sim}8$. SHE was partitioned in the order of n-hexane, chloroform, ethyl acetate and butanol. As the results of the MIC test for each obtained fraction, no fraction exhibited higher antibacterial activity than that of the crude SHE. However, a mixture of chloroform, ethylacetate and ethanol fractions showed higher antibacterial activity than SHE.
Keywords
algae; Sargassum thunbergii; antibacterial activity;
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1 Chang DS, Shin DH, Chung DH, Kim CM, Lee IS. 2002. Food hygiene. Jungmoongak, Seoul. p 244-246
2 Ferrand C, Marc F, Fritsch P. 2000. Chemical and toxicological studies of products resulting from sorbic acid and methylamine interaction in food conditions. Amino Acids 18: 251-263   DOI
3 Buchanan RL, Shepherd AJ. 1981. Inhibition of Aspergillus parasiticus by thymol. J Food Sci 46: 976-977   DOI
4 Korea food and drug administration. 2005. Statistics of outbreak of food poisoning. Ministry of health and welfare
5 Cherry JP. 1999. Improving the safety of fresh produce with antimicrobials. Food Technol 53: 54-59
6 Choi HY, Han YS. 2003. Isolation and Identification of antimicrobial compound from dansam (Salivia miltiorrhiza Bunge). J Korean Soc Food Sci Nutr 32: 22-28   과학기술학회마을   DOI   ScienceOn
7 Sim CJ, Lee GH, Jung JH, Yi SD, Kim YH, Oh MJ. 2004. Isolation and identification of antimicrobial activity substances from Rhodiola sachlinensis. Kor J Food Preserv 11: 63-70
8 Awad NE, Selim MA, Saleh MM, Matloub AA. 2003. Seasonal variation of the lipoidal matters and hypolipidaemic activity of the red alga Corallina officinalis L. Phytother Res 17: 19-25   DOI   ScienceOn
9 Ryu BH, Kim DS, Cho K, Sin DB. 1989. Antitumor activity of seaweeds agarne Sarcoma-180. Kor J Food Sci Technol 21: 595-600   과학기술학회마을
10 Liu JN, Yoshida Y, Wang MQ, Okai Y, Yamachita UB. 1997. B cell stimulating activity of seaweed extracts. Int J Immunopharmac 19: 135-142   DOI   ScienceOn
11 Lee HJ, Kim JH, Lee CH, Kim JS, Kwak ST, Lee KB, Song KS, Choi BW, Lee BH. 1999. Inhibitory activities of sea weeds on prolyl endopeptidase, tyrosinase and coagulation. Kor J Pharmacogn 30: 231-237
12 Kuda T, Kunii T, Goto H, Suzuki T, Yano T. 2007. Varieties of antioxidant and antibacterial properties of Ecklonia kurome products harvested and processed in the Noto Peninsula, Japan. Food Chem 103: 900-905   DOI   ScienceOn
13 Kim SH, Lim SB, Ko YH, Oh MC, Park CS. 1994. Extraction yields of Hizikia fusiforme by solvents and their antimicrobial effects. Bull Korean Fish Soc 27: 462-468   과학기술학회마을
14 Amico G, Oriente G, Piatelli M, Tringali C, Fattorusso E, Mango S, Mayol L. 1980. Diterpenes based on the dolabellane skeleton from Dictyota dichotoma. Tetrahedron 36: 1409-1414   DOI   ScienceOn
15 Glombitza KW, Rosener HU, Vilter H, Rauwald W. 1973. Antibiotics from algae. 8. Phloroglucinol from Phaeophyceae. Planta Med 24: 301-303   DOI
16 Lim CW, Lee JS, Cho YJ. 2000. Structure and some properties of the antimicrobial compounds in the red alga, Symphyosladia latiscula. J Korean Fish Soc 33: 280-287
17 Montes-Belmont R, Carvajal M. 1998. Control of Aspergillus flavus in maize with plant essential oil and their components. J Food Prot 61: 616-619   DOI
18 Hwang JS, Han YS. 2003. Isolation and identification of antimicrobial compound from Mokdan bark (Paeonia suffruticosa ANDR). J Korean Soc Food Sci Nutr 32: 1059-1065   과학기술학회마을   DOI   ScienceOn
19 Val AG, Platas G, Basilio A, Cabello A, Gorrochategui J, Suay I, Vicente F, Portillo M, Rio MJ, Reina GG, Pelaez F. 2001. Screening of antimicrobial activities in red, green and brown macroalgae from Gran Canaria (Canary islands, Spain). Int Microbiol 4: 35-40
20 Murakami M, Makabe K, Okada S, Yamaguchi K, Konosu S. 1988. Screening of biologically activity compounds in microalgae. Nippon Suisan Cakki Shi 54: 1035-1039   DOI
21 Nadal NGM, Casillias CM, Rodriguez LV, Rodriguez JR, Vera LT. 1966. Antibiotic properties of marine algae-III. Cymopolia barbara. Bot Marina 9: 121-126
22 Nakamura S, Kato AM, Kobayashi K. 1991. New antimicrobial characteristics of lysozyme-dextran conjugate. J Agric Food Chem 39: 647-650   DOI
23 Kubo I, Himejima M, Tsujimoto K, Muroi H, Ichikawa N. 1992. Antibacterial activity of crinitol and its potentiation. J Nat Prod 55: 780-785   DOI
24 Park CS, Cha MS. 2000. Comparison of antibacterial activity of green tea extracts and preservation to the pathogenic bacteria. Korean J Food Nutr 13: 36-44   과학기술학회마을
25 Magallanes C, Cordoba C, Orozco R. 2003. Antimicrobial activity of ethanolic extracts of marine algae from central coast of Peru. Rev Peru Biol 10: 125-132
26 Iwashima M, Mori J, Ting X, Matsunaga T, Hayashi K, Shinoda D, Saito H, Sankawa U, Hayashi T. 2005. Antioxidant and antiviral activities of plastoquinones from the brown alga Sargassum micracanthum, and a new chromene derivative converted from the plastoquinones. Biol Pharm Bull 28: 374-377   DOI   ScienceOn
27 Kim JY, Kim KH, Suh HS, Choi WC. 1997. Antiinflammatory effects of new chemical compounds, HS-1580 series (HS-1580, HS-1581, HS-1582). J Life Sci 16: 1181-1187
28 Chimenti F, Bizzarri B, Bolasco A, Secci D, Chimenti P, Carradori S, Granese A, Rivanera D, Lilli D, Zicari A, Scaltrito MM, Sisto F. 2007. A novel class of selective anti-Helicobacter pylori agents 2-one-2H-chromene-3-carboxamide derivatives. Bioorg Med Chem Lett 17: 3065-3071   DOI   ScienceOn
29 Zhuang C, Itoh H, Mizuno T. 1995. Antitumor active fucoidan from the brown seaweed, umitoranoo (Sargassum thunbergii). Biosci Biotech Biochem 59: 563-567   DOI   ScienceOn
30 Kim JH, Lee DS, Lim CW, Park HY, Park JH. 2002. Antibacterial activity of sea-mustard, Laminaria japonica extracts on the cariogenic bacteria, Streptococcus mutans. J Korean Fish Soc 35: 191-195   과학기술학회마을   DOI
31 Kang SY, Oh MJ, Shin JA. 2005. Antimicrobial activity of korean marine algae against fish pathogenic bacteria. J Fish Pathol 18: 147-156
32 Park UY, Kim SH, Kim JH, Kim YG, Chang DS. 1995. Purification of antimicrobial substance for the extract from the root bark of Morus alba. J Fd Hyg Safety 10: 225-230   과학기술학회마을
33 Kim JH, Lee DS, Lim CW, Park HY, Park JH. 2002. Antibacterial activity of sea-mustard, Laminaria japonica extracts on the cariogenic bacteria, Streptococcus mutans. J Korean Fish Soc 35: 191-195   과학기술학회마을   DOI
34 Kong MY, Kang TS, Lee MK, Park BK, Oh DH. 2001. Antimicrobial and antioxidative activities of solvent fractions of Quercus mongolica leaf. J Korean Soc Food Sci Nutr 30: 415-420   과학기술학회마을
35 Seo YW, Park KE, Nam TJ. 2007. Isolation of a new chromene from the brown alga Sargassum thunbergii. Bull Korean Chem Soc 28: 1831-1833   DOI   ScienceOn
36 Yin MC, Cheng WS. 1998. Inhibition of Aspergillus niger and Aspergillus flavus by some herbs and spices. J Food Prot 61: 123-125   DOI
37 Ouattara B, Simard RE, Holley RA, Piette GJP, Begin A. 1997. Antibacterial activity of selected fatty acids and essential oil against six meat spoilage organisms. Int J Food Microbiol 37: 155-162   DOI   ScienceOn
38 Yang F, Wang Lin, Hu Qiuhui. 2005. Preparation of polysaccharide derived from Sargassum thunbergii and its antioxidant activity. Food Sci 26: 224-227
39 Choi JS, Park HJ, Jung HA, Chung HY, Jung JH, Choi WC. 2000. A cyclohexanonyl bromophenol from the red alga Symphyocladia latiuscula. J Nat Prod 63: 1705-1706   DOI   ScienceOn
40 Fenical W, Sims JJ. 1973. Zonarol and isozonarol, fungitoxic hydroguinones from the brown seaweed Dictyopteris zonarioides. J Org Chem 38: 2383-2386   DOI
41 Seo Y, Lee HJ, Park KE, Kim YA, Ahn JW, Yoo JS, Lee BJ. 2004. Peroxynitrite-scavenging constituents from the brown alga Sargassum thunbergii. Biosci Biotech Eng 9: 212-216   과학기술학회마을   DOI   ScienceOn
42 Lee JH, Lee KH, Yoo HI, Zhou XL, Kim YS, Han GC, Nam KW. 2006. Antimicrobial activity of Neorhodomela aculeata extracts against human skin pathogens. J Kor Fish Soc 39: 292-296   과학기술학회마을   DOI
43 EI-Shenawy MA, Marth EH. 1989. Behavior of Listeria monosytogenes in the presence of sodium propionate. J Food Microbiol 8: 85-92   DOI   ScienceOn
44 Morales JL, Cantillo-Ciau ZO, Sanchez-Molina I, Mena-Rejon GJ. 2006. Screening of antibacterial and antifungal activity of six marine macroalgae from coasts of Yucatan Peninsula. Pharm Biol 44: 632-635   DOI   ScienceOn
45 Yoon JW, Yoo MY, Choi JH, Lee MK, Oh DH. 2005. Antimicrobial effects of ethanol extracted and sub-fractionated materials from different parts of Quercus aliena Blume. J Korean Soc Food Sci Nutr 34: 910-914   과학기술학회마을   DOI   ScienceOn
46 Kim YH, Kim EH, Lee CH, Kim MH, Rho JR. 2007. Two new monogalactosyl diacylglycerols from brown alga Sargassum thunbergii. Lipids 42: 395-399   DOI