• Journal of Microbiology
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• v.40 no.2
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• pp.173-177
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• 2002

In order to identify Yawowia lipolytica genes induced by serum, cDNA representational difference analysis was performed using a PCR-select CDNA subtraction method. One of the genes cloned from the subtraction was a gene (YIMBFl) homologous to Saccharomyces cerevisiae MBF1 encoding the coactivator multiprotein bridging factor 1. Disruption of YIMBFl revealed that the gene was net essential for viability, and the Ylmbf△ strain did not show any distinct phenotypic change on solid serum medium. In liquid medium, however. a difference was found in the ability to maintain hyphae induced by serum. This result suggests that the YIMbf1 protein may mediate transcriptional activation of certain genes involved in the hypha fonmation of Y. lipolytica.

• Journal of the Korean Society for Aviation and Aeronautics
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• v.29 no.2
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• pp.84-93
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• 2021

Airline schedule is the most basic data for flight operations and has significant importance to an airline's management. It is crucial to know the airline's current schedule status in order to effectively manage the company and to be prepared for abnormal situations. In this study, machine learning techniques were applied to actual schedule data to examine the possibility of whether the airline's fleet state could be artificially learned without prior information. Given that the schedule is in categorical form, One Hot Encoding was applied and t-SNE was used to reduce the dimension of the data and visualize them to gain insights into the airline's overall fleet status. Interesting results were discovered from the experiments where the initial findings are expected to contribute to the fields of airline schedule health monitoring, anomaly detection, and disruption management.

• Land and Housing Review
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• v.11 no.4
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• pp.115-124
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• 2020

Incentive/Disincentive (I/D) contract is designed for minimizing any disruption of traffic flow in road construction projects. I/D contracting projects have been evaluated with regard to time and cost performance in various states, more than 35 states. However, construction project managers and planners have little understanding of the project factors that affect the project time and cost performance of highway construction projects using I/D regulations. Therefore, the purpose of this research is to find factors that affect I/D project success or failure to improve the decision-making process for the implementation of I/D projects. In order to achieve the objectives of this research, the researchers collected I/D road construction project data from FDOT and performed evaluation for each collected project. Then, project data analysis to identify key factors that affect I/D project performance was performed. In conclusion, five significant factors for project time performance and six significant factors for project cost perfornace were identified and summarized.

• International conference on construction engineering and project management
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• 2005.10a
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• pp.886-891
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• 2005

The frequent maintenance required on some asphalt concrete (AC) pavement sections has made reconstruction with Portland Cement Concrete Pavement (PCCP) a feasible alternative. However, many constructability issues need to be addressed in order to realize the full potential of this alternative. Accelerated paving encompasses three classes of activities: methods to accelerate the rate of strength gain, methods to minimize the construction time, and traffic control strategies to minimize user delay. Three major AC intersections with severe rutting problems were reconstructed with PCCP. The entire reconstruction of each intersection, including demolition of the AC pavement and its replacement with PCCP, took place over a period of three days, starting on Thursday evening and opening the intersection to the traffic on Sunday afternoon. This paper discusses the use of PCCP for accelerated reconstruction of major urban intersections with minimal user and traffic disruption, using innovative construction techniques and traffic management optimization principles and demonstrates that concrete pavements can be constructed efficiently and quickly.

• Journal of Environmental Science International
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• v.21 no.7
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• pp.845-853
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• 2012

Diethyl phthalate (DEP) is widely spread in the natural environment as an endocrine disruption chemicals (EDs). Therefore, in this study, ultrasound (US) and ultraviolet (UVC), including various applied power density (10-40 W/L), UV wavelengths (365 nm, 254 nm and 185 nm) and frequencies (283 kHz, 935 kHz) were applied to a DEP contaminated solution. The pseudo-first order degradation rate constants were in the order of $10^{-1}$ to $10^{-4}\;min^{-1}$ depending on the processes. Photolytic and sonophotolytic DEP degradation rate also were high at shortest UV wavelength (VUV) due to the higher energy of photons, higher molar absorption coefficient of DEP and increased hydroxyl radical generation from homolysis of water. Sonolytic DEP degradation rate increased with increase of applied input power and the dominant reaction mechanism of DEP in sonolysis was estimated as hydroxyl radical reaction by the addition of t-BuOH, which is a common hydroxyl radical scavenger. Moreover, synergistic effect of were also observed for sonophotolytic degradation with various UV irradiation.

• Journal of the Korean Society for Advanced Composite Structures
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• v.6 no.3
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• pp.41-49
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• 2015

The repair of manhole raise has been caused much construction times and disruption of traffic flow, serious environmental pollution from crushed construction wastes, and budget waste due to the repeated repair construction works. In order to overcome such problems, we have developed the new manhole repairing composite structures by using a glass fiber-reinforced polymer (GFRP) pipe, which can raise manhole to the regular height of the overlayed road pavement with rapid construction and minimum traffic jams. This environmental-friendly technology is method completed by the methyl methacrylate monomer (MMA) double wide flanged GFRP pipe composite structures in order to raise manhole to the regular height. In this paper, two kinds of the compressive strength tests of MMA mortar composites were conducted and evaluated by a general compressive strength test, and compressive strength test after freezing-thawing resistance test. It was found that this MMA mortar composites will be used for the application of the double wide flanged GFRP pipe composite structures.

• Proceedings of the Membrane Society of Korea Conference
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• 1997.10a
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• pp.39-45
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• 1997

Introduction : Polyimides are one of the most important classes of the high performance polymers due to their excellent electrical, thermal, and high-temperature mechanical properties. But their uses are limited because of their poor solubility. Most polyimide derivatives are processed in the form of polyamic acids, which are subsequently converted into the imide structures.Recently, it has been found that the soluble polyimides with large molecular weight sufficient to application. For enhancing processability, the majority of approaches have involved the following factors. As much as, the separation of the imide ring along the back-bone, that is to say, reducing the density of imide ring in the repeat structure. The introduction of bulky substituents along the back-bone, in order to enhance the free volume of main-chain. The incorporation of flexible or thermally stable linkages in the main-chain, reducing the packing force. The disruption of symmetry or recurrence regularity through copolymerization in order to reduce crystallnity.The objectives of this investigation are the synthesis and characterization of soluble polyimides as membrane materials by the single-step polymerization and the preparation of the asymmetric polyimide membrane by using phase inversion technique. In the present study, three series of polyimide derivatives are synthesized; H series is homopolyimides, A series is prepared from single dianhydride and two diamines, B series is yielded from two dianhydrides and a diamine. The dope solution was directly prepared from the PI solution via one step polymerization from monomers.

• Journal of Urban Science
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• v.8 no.1
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• pp.33-44
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• 2019

The purpose of this study is to investigate the cause of repeated accidents through the investigation and analysis of the collapse cases of domestic earthquakes in order to prevent the earthquake disaster in the urban redevelopment and reconstruction. It is designed and constructed to draw out various problem factors and to find solutions to these problems. And the contents related to various laws and systems of the construction project management stage. Especially. In the management of the construction management centered on the safety management and quality control of the technical aspect design, supervision, construction phase through the cause of the accident and improvement measures based on the investigation report of the collapse of the construction site near Sangdo Kindergarten in Seoul. Supervisor. And constructors should be settled on the responsibility and responsibility of God. In order to achieve the purpose of the project, a system is established to link worker-centered occupational safety and health management with technology-based safety construction management, and analysis of how the quality control of the earthquake prevention affects safety construction management The purpose of this study is to prepare the optimum management plan of construction business.As a result of this study, it is aimed to accomplish the purpose of small scale redevelopment and reconstruction construction project by providing optimization method in the stage of construction project management in order to prevent collapse caused by differences in design,Is expected.

• Journal of Microbiology
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• v.44 no.6
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• pp.649-654
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• 2006

A standard type II polyketide synthase (PKS) gene cluster was isolated while attempting to clone the biosynthetic gene for lipstatin from Streptomyces toxytricini NRRL 15,443. This result was observed using a Southern blot of a PstI-digested S. toxytricini chromosomal DNA library with a 444 bp amplified probe of a ketosynthase (KS) gene fragment. Four open reading frames [thioesterase (TE), $\beta$-ketoacyl systhase (KAS), chain length factor (CLF), and acyl carrier protein (ACP)], were identified through the nucleotide sequence determination and analysis of a 4.5 kb cloned DNA fragment. In order to confirm the involvement of a cloned gene in lipstatin biosynthesis, a gene disruption experiment for the KS gene was performed. However, the resulting gene disruptant did not show any significant difference in lipstatin production when compared to wild-type S. toxytricini. This result suggests that lipstatin may not be synthesized by a type II PKS.

• Journal of Microbiology and Biotechnology
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• v.18 no.3
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• pp.397-403
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• 2008

Nikkomycins are a group of peptidyl nucleoside antibiotics with potent fungicidal, insecticidal, and acaricidal activities. sanN was cloned from the partial genomic library of Streptomyces ansochromogenes 7100. Gene disruption and complementation analysis demonstrated that sanN is essential for nikkomycin biosynthesis in S. ansochromogenes. Primer extension assay indicated that sanN is transcribed from two promoters (sanN-P1 and sanN-P2), and sanN-P2 plays a more important role in nikkomycin biosynthesis. Purified recombinant SanN acts as a dehydrogenase to convert benzoate-CoA to benzaldehyde in a random-order mechanism in vitro, with respective $K_{cat}/K_m$$ values of $3.8mM^{-1}s^{-1}\;and\;12.0mM^{-1}s^{-1}$ toward benzoate-CoA and NADH, suggesting that SanN catalyzes the formation of picolinaldehyde during biosynthesis of nikkomycin X and Z components in the wild-type stain. These data would facilitate us to understand the biosynthetic pathway of nikkomycins and to consider the combinatorial synthesis of novel antibiotic derivatives.