Browse > Article

Cloning and characterization of the multiprotein bridging factor 1 (YIMBFI) gene from the dimorphi yeast Yarrowia lipolytica  

Kim, Jeong-Yoon (Department of Microbiology, Chungnam University, DaeJeon 305-764)
Kim, Jang-Hwan (Department of Microbiology, Chungnam University, DaeJeon 305-764)
Cheon, Seong-Ah (Department of Microbiology, Chungnam University, DaeJeon 305-764)
Yunkyoung Song (Department of Microbiology, Chungnam University, DaeJeon 305-764)
Publication Information
Journal of Microbiology / v.40, no.2, 2002 , pp. 173-177 More about this Journal
Abstract
In order to identify Yawowia lipolytica genes induced by serum, cDNA representational difference analysis was performed using a PCR-select CDNA subtraction method. One of the genes cloned from the subtraction was a gene (YIMBFl) homologous to Saccharomyces cerevisiae MBF1 encoding the coactivator multiprotein bridging factor 1. Disruption of YIMBFl revealed that the gene was net essential for viability, and the Ylmbf△ strain did not show any distinct phenotypic change on solid serum medium. In liquid medium, however. a difference was found in the ability to maintain hyphae induced by serum. This result suggests that the YIMbf1 protein may mediate transcriptional activation of certain genes involved in the hypha fonmation of Y. lipolytica.
Keywords
Yarrowia lipolyrica; multiprotein bridging factor; MBF1; serum;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
Times Cited By Web Of Science : 3  (Related Records In Web of Science)
Times Cited By SCOPUS : 1
연도 인용수 순위
1 Improvement of heterologous protein productivity using recombinant Yarrowia lipolytica and cyclic fed-batch process strategy /
[ Chang, C.C.;D.D. Ryu;C.S. Park;J.Y. Kim ] / Biotechnol. Bioeng.   DOI   ScienceOn
2 Yeast coactivator MBF1 mediates GCN4-dependent transcriptional activation /
[ Takemaru, K.;S. Harashima;H. Ueda;S. Hirose ] / Mol. Cell. Biol.
3 PCR- and ligation-mediated cynthesis of split-marker cassettes with long flanking homology regions for gene disruption in Candida albicans /
[ de Hoogt, R.;W.H. Luyten;R. Contreras;M.D. De backer ] / Biotechniques
4 Dimorphism and virulence in Candida albicans /
[ Mitchell, A.P. ] / Curr. Opin. Microbiol.   DOI   ScienceOn
5 Cryptosporidium parvum: functional complementation of a parasite transcriptional coactivator CpMBF1 in yeast /
[ Zhu, G.;M.J. LaGier;S. Hirose;J.S. Keithly ] / Exp. Parasitol.   DOI   ScienceOn
6 MHY1 encodes a C2H2-type zinc finger protein that promotes dimorphic transition in the yeast Yarrowia lipolytica /
[ Hurtado, C.A.;R.A. Rachubinski ] / J. Bacteriol.
7 Nonfilamentous C. albicans mutants are avirulent /
[ Lo, H.J.;J.R. Kohler;B. Didomenico;D. Loebenberg;A. Cacciapuoti;G.R. Fink ] / Cell   DOI   ScienceOn
8 Multiprotein bridging factor 1 (MBF1) is an evolutionarily conserved transcriptional coactivator that connects a regulatory factor and TATA element-binding protein /
[ Takemaru, K.;F.Q. Li;H. Ueda;S. Hirose ] / Proc. Natl. Acad. Sci.   DOI   ScienceOn
9 Yarrowia lipolytica /
[ Barth, G.;C. Gaillardin;Wolf, K.(ed.) ] / Nonconventional yeasts in biotechnology
10 CLUSTALW: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice /
[ Thompson, J.D.;D.G. Higgins;T.J. Gibson ] / Nucleic Acids Res.   DOI   ScienceOn
11 Comparison of promoters suitable for regulated overex-pression of β-galactosidase in the alkane-utilizing yeast Yarrowia lipolytica /
[ Juretzek, T.;H. Wang;J. Nicaud;S. Mauersberger;G. Barth ] / Biotechnol. Bioprocess Eng.   과학기술학회마을   DOI
12 Candida albicans and Yarrowia lipolytica as alternative models for analysis budding patterns and germ tube formation in dimorphic fungi /
[ Herrero, A.B.;M.C. Lopez;l. Fernandez-Lago;A. Dominguez ] / Microbiology
13 Seruminduced hypha formation in the dimorphic yeast Yarrowia lipolytica /
[ Kim, J.;S.A. Cheon;S. Park;Y. Song;J.Y. Kim ] / FEMS Microbiol. Lett.   DOI   ScienceOn
14 HOY1, a homeo gene required for hyphal formation in Yarrowia lipolytica /
[ Torres-Guzman, J.C.;A. Dominguez ] / Mol. Cell. Biol.
15 The role of human MBF1 as a transcriptional coactivator /
[ Kabe, Y.;M. Goto;D. Shima;T. Imai;T. Wada;K. Morohashi;M. Shirakawa;W. Hirose;H. Handa ] / J. Biol. Chem.   DOI
16 Ras signaling is required for serum-induced hyphal differentiation in Candida albicans /
[ Feng, Q.;E. Summers;B. Guo;G. Fink ] / J. Bacteriol
17 Tagging morphogenetic genes by insertional mutagenesis in the yeast Yarrowia lipolytica /
[ Richard, M.;R.R. Quijano;S. Bezzate;F. Bordon-Pallier;C. Gaillardin ] / J. Bacteriol.   DOI   ScienceOn
18 Factors affecting the morphogenetic switch in Yarrowia lipolytica /
[ Perez-Campo, F.M.;A. Dominguez ] / Curr. Microbiol.   DOI   ScienceOn
19 Control of pseudohyphae formation in Saccharomyces cerevisiae /
[ Gancedo, J.M. ] / FEMS Microbiol. Rev.   DOI   ScienceOn
20 EDF-1, a novel gene product down-regulated in human endothelial cell differentiation /
[ Dragoni, I.;M. Mariotti;G.G. Consalez;M.R. Soria;J.A. Maier ] / J. Biol. Chem.   DOI   ScienceOn
21 Physiology and genetics of the dimorphic fungus Yarrowia lipolytica /
[ Barth, G.;C. Gaillardin ] / FEMS Microbiol. Rev.   DOI   ScienceOn
22 Expression, secretion, and processing of rice alpha-amylase in the yeast Yarrowia lipolytica /
[ Park, C.S.;C.C. Chang;J.Y. Kim;D.M. ogrydziak;D.D. Ryu ] / J. Biol. Chem.   DOI   ScienceOn