• Title/Summary/Keyword: Optimal manufacture conditions

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Optimization of Culture Conditions for the Yeast and Analysis of Qualities of Makgeolli Brewed with the Yeast Isolated from Korean Traditional Nuruk (전통누룩으로부터 막걸리용 효모의 선별 및 최적 배양조건)

  • Kang, Hyang-Rin;Lee, Ae-Ran;Kwon, Young-Hee;Kim, Jae-Ho;Kim, Hye-Ryun;Ahn, Byung-Hak
    • The Korean Journal of Mycology
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    • v.40 no.4
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    • pp.204-209
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    • 2012
  • In this study, a novel yeast, Y111-5 for Makgeolli manufacture was selected from Nuruk yeasts, and its optimal culture condition were investigated. The Y111-5 strain was identified as Saccharomyces cerevisiae by phylogenetic analysis of 18S RNA sequence. The maximal growth was obtained when the yeast was cultivated at $30^{\circ}C$ for 15 h in the medium containing sucrose 9% and yeast extract 5%.

The fabrication and the analysis on a communication device for bilateral (양방향 통신 장치 제작 및 분석)

  • You, Il-hyun
    • Journal of Convergence for Information Technology
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    • v.8 no.2
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    • pp.83-90
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    • 2018
  • We have studied the optimal conditions for design and development on the communication device for a bilateral, and it's electrodes for transmitting electric signal are constructed on the $36^{\circ}$ rotated $LiTaO_3$ substrate by evaporating Al-Cu(W 30%) alloy. At first, we manufactured three kind of samples using this method, and selected two samples as similar with frequency, ripple and passband characteristics, and then we connect two samples by series in order to make bilateral devices. As results, we obtained that the electrode structure has better characteristics then the others, when it's width of reflector and electrode are $1{\lambda}/4$, $1{\lambda}/12$ respectively, and it's frequency is approximately 190.3MHz. Near future, I hope to help the manufacture for communication devices for the multi-channel and the duplex filter.

A Study on Bond Wire Fusing Analysis of GaN Amplifier and Selection of Current Capacity Considering Transient Current (GaN증폭기의 본드 와이어 용융단선 현상분석과 과도전류를 고려한 전류용량 선정에 대한 연구)

  • Woo-Sung, Yoo;Yeon-Su, Seok;Kyu-Hyeok, Hwang;Ki-Jun, Kim
    • Journal of IKEEE
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    • v.26 no.4
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    • pp.537-544
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    • 2022
  • This paper analyzes the occurrence and cause of bond wires fusing used in the manufacture of pulsed high power amplifiers. Recently GaN HEMT has been spotlight in the fields of electronic warfare, radar, base station and satellite communication. In order to produce the maximum output power, which is the main performance of the high-power amplifier, optimal impedance matching is required. And the material, diameter and number of bond wires must be determined in consideration of not only the rated current but also the heat generated by the transient current. In particular, it was confirmed that compound semiconductor with a wide energy band gap such as GaN trigger fusing of the bond wire due to an increase in thermal resistance when the design efficiency is low or the heat dissipation is insufficient. This data has been simulated for exothermic conditions, and it is expected to be used as a reference for applications using GaN devices as verified through IR microscope.

The study on the metabolism of benzidine in the isolated perfused rat liver (흰쥐의 적출 간 관류법을 이용한 벤지딘 대사에 관한 연구)

  • Bae, Mun Joo;Roh, Jae Hoon;Cho, Young Bong;Kim, Choon Sung;Chun, Mi Ryoung;Kim, Chi Nyon
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.6 no.1
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    • pp.28-37
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    • 1996
  • Benzidine, an aromatic amine used primarily in the manufacture of azo dyes, is recognized as a urinary bladder carcinogen in humans. In rats, mice, and hamsters, chronic exposure to benzidine resulted in tumors of the liver. The present study was undertaken to suggest analyzing the metabolites of benzidine with the optimal condition, identify the metabolites of benzidine, and observe time variance of the metabolites in the isolated perfusated rat liver. N-acetylbenzidine was synthesized by acetylation of benzidine with acetic anhydride and separated by thin layer chromatography(TLC) and high performance liquid chromatography(HPLC). To analysis benzidine and the metabolites of benzidine, HPLC operating condition has been optimized by means of preliminary experiment. The mobile phase consisted of acetonitrile(37%) in phosphate buffer, flow rate maintained at 1.0 ml/min. Optimal detective conditions were electrochemicaldetector(ECD) at 0.75 V for benzidine and N-acetylbenzidine and ultravioletdetector(UVD) at 287 nm for N,N'-diacetylbenzidine. The separation system was composed of a guard column and a separation column(Polymer C18, $4.6{\times}250cm$) at a temparature of $40^{\circ}C$. The perfusion system was equilibrated for 30 minutes before addition of benzidine to the perfusate. Samples of the perfusate were collected at time intervals(0, 10, 20, 30, 60, 90, 120 min) during the 2 hour perfusion. Before analyzing samples by HPLC/ECD/UVD, samples had been treated with sep-pak. Samples of perfusate analyzed by HPLC/ECD/UVD and the metabolites of benzidine in the isolated perfused rat liver were N-acetylbenzidine and N,N'-diacetylbenzidine. Benzidine metabolized over 60% during the initial 30 minutes of perfusion, extensively by 1 hour, and was undetectable in the perfusate. N-acetylbenzidine increased by 30 minutes of perfusion, declined. N,N'-diacetylbenzidine increased the 0-90 minutes period, remained constant during the 90-120 minutes period.

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The Characteristics of Manufacture Filter Media for Water Treatment Using Mixture Response with Ash and Food Waste (연소재 및 식품폐기물의 혼합 반응에 따른 수처리 여과재 제조 특성)

  • Park, Seung-Do;Lee, Won-Ho;Lee, Min-Hee
    • Journal of the Korean GEO-environmental Society
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    • v.19 no.5
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    • pp.5-12
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    • 2018
  • The porosity formation by the addition of additives was found to be the highest in the case of aluminum powder 3% and $Ca(OH)_2$ 2% under the condition that strength was maintained. The optimum mixing ratio of the binder was shown to be the most effective at (Ash+Food waste+clay):(water glass+colloidal silica) 7:3, and the temperature response is most economical and effective at $1,000^{\circ}C$. The optimal mixing ratio is the strength in 30% of ash, 30% of clay and 10% of food waste, which is the effective in non-point pollution water treatment. Filter media produced under optimal mixing conditions were analyzed as $SiO_2$ 65.8%, density $1.4g/cm^3$, porosity 25.6%, pH 9.8, and no hazardous substances were detected. As a result of the filtration of the water treatment, the mean concentration of the filtered SS was $14.06mg/{\ell}$, and the removal efficiency of SS was 90%, the recovery rate of the reversal is 97.1%. This enables the development of filter media considering economic efficiency and efficiency as well as the utilization of waste resources, enabling high value added of waste resources.

Optimization and Scale-up of Fish Skin Peptide Loaded Liposome Preparation and Its Storage Stability (어피 펩타이드 리포좀 대량생산 최적 조건 및 저장 안정성)

  • Lee, JungGyu;Lee, YunJung;Bai, JingJing;Kim, Soojin;Cho, Youngjae;Choi, Mi-Jung
    • Food Engineering Progress
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    • v.21 no.4
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    • pp.360-366
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    • 2017
  • Fish skin peptide-loaded liposomes were prepared in 100 mL and 1 L solution as lab scales, and 10 L solution as a prototype scale. The particle size and zeta potential were measured to determine the optimal conditions for the production of fish skin peptide-loaded liposome. The liposome was manufactured by the following conditions: (1) primary homogenization at 4,000 rpm, 8,000 rpm, and 12,000 rpm for 3 minutes; (2) secondary homogenization at 40 watt (W), 60 W, and 80 W for 3 minutes. From this experimental design, the optimal conditions of homogenization were selected as 4,000 rpm and 60 W. For the next step, fish peptides were prepared as the concentrations of 3, 6, and 12% at the optimum manufacturing conditions of liposome and stored at $4^{\circ}C$. Particle size, polydispersion index (pdI), and zeta potential of peptide-loaded liposome were measured for its stability. Particle size increased significantly as manufacture scale and peptide concentration increased, and decreased over storage time. The zeta potential results increased as storage time increased at 10 L scale. In addition, 12% peptide showed the formation of a sediment layer after 3 weeks, and 6% peptide was considered to be the most suitable for industrial application.

Design of a shoe rack for effective sterilization and deodorization of the shoes contaminated by various bacteria (오염된 신발의 효율적인 살균과 탈취를 위한 신발장의 설계)

  • Lee, Sam-Cheol;Jang, Yong-Seok
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.17 no.6
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    • pp.199-206
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    • 2016
  • The aim of this study was to establish the optimal conditions to design a shoe rack that could effectively sterilize and deodorize shoes contaminated by various bacteria. The manufacture and evaluation of a shoe rock was conducted for one year from October 2014 to September 2015. The antibacterial efficacies were evaluated using Escherichia coli and Staphylococcus aureus by JIS Z 2801:2010. The deodorization efficiency was identified by the standard method for deodorization efficiency evaluation of an indoor air cleaner established by Korea Air Cleaning Association, SPS-KACA002-132:2006. The results showed that antibacterial evaluation showed more than 99.9% of the sterilization effects on Escherichia coli and Staphylococcus aureus after exposure to ultraviolet (UV) light for 30 seconds. The average deodorization efficiency for the test gases was 42.5%. The temperature in the shoes and in the shoe rack was lower than $40^{\circ}C$ and $25^{\circ}C$, respectively, when the UV lamp was turned on for 5 minutes and off for 25 minutes repeatedly. This was controlled by turning the UV lamp on and off to maintain the appropriate temperature in the shoes. In conclusion, the real deodorization efficiency is expected to be higher than the measured value because the internal volume of the shoe rack was smaller than the test chamber used to measure the deodorization efficiency. The deodorization effect could be increased further by the sterilization of bacteria causing bad odors in shoes. Further studies will be needed to determine the temperature deviations within the shoe rack to provide constant conditions.

Preparation and Characterization of High Absorptive Cellulose Film Derived from Styela Clava Tunic for Wound Dressing (흡수성이 우수한 창상치료용 미더덕껍질 셀룰로오스필름의 제조 및 특성 분석)

  • Seong, Keum-Yong;Koh, Eun-Kyoung;Lee, Seunghyun;Kwak, Moon Hwa;Son, Hong Joo;Lee, Hee Seob;Hwang, Dae Youn;Jung, Young Jin
    • Textile Coloration and Finishing
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    • v.27 no.1
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    • pp.70-79
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    • 2015
  • To establish the optimal conditions for the manufacture of high absortive cellulose film(CF) originated from Styela clava tunic(SCT), the physicochemical properties included absorption was measured in CFs prepared under the various conditions. The highest absorption was observed for SCT-CF20 prepared from the cellulose solution dissolved at $80^{\circ}C$ for 20min, although the filtration treatment did not induce any significance alteration. Also, the absorption was higher in SCT-CF20-F prepared by the freeze drying than SCT-CF20-N(ambient air drying) and SCT-CF20-H(heat drying). The addition of porogen($NaHCO_3$) induced an increase of absorption in SCT-CF20-PF relative to SCT-CF20-F, while the number of interconnected porous structures was enhanced in SCT-CF20-PF. Furthermore, a high level of SK-MEL-2 cells viability was observed in SCT-CF20-PN and SCT-CF20-PF cultured group. These results show that SCT-CF may have high absorption and biocompatibility when prepared from SCT cellulose solution dissolved at $80^{\circ}C$ for 20min after addition of porogen and then subjected to freeze drying.

Effect of Rice Seed Disinfection of Loess-sulfur on the Suppression of Bakanae disease caused by Fusarium fujikuroi (벼 키다리병 방제에 관한 황토유황의 종자소독 효과)

  • So, Hyun-Kyu;Kim, Yong-Ki;Hong, Sung-Jun;Han, Eun-Jung;Park, Jong-Ho;Shim, Chang-Ki;Kim, Min-Jeong;Kim, Seok-Cheol
    • Korean Journal of Organic Agriculture
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    • v.25 no.2
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    • pp.345-355
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    • 2017
  • This study was conducted to evaluate rice seed disinfection efficacy of loess-sulfur for the suppression of Bakanae disease caused by Fusarium fujikuroi. Rice seeds were treated at different concentrations of loess-sulfur, soaking time and temperature, and combination of hot-water treatment. Rice cultivar, Shindongjin harvested from Bakanae disease-infested area in 2015, was used. Loess-sulfur was treated as follows; concentration of undiluted solution, 2%, 1% and 0.5%; soaking time of 24 and 48 hours; treatment temperature of $20^{\circ}C$ and $30^{\circ}C$; hot water treatment or not. Optimal conditions of rice seed disinfection were selected soaking time of 48 hours and the suspension of 0.5% and 1% loess-sulfur by investigating seed germination and isolation frequency of Fusarium spp. on Komada agar medium in vitro, and were established 3 disinfection conditions as hot water ($60^{\circ}C$, 10 min.) + 1% loess-sulfur ($20^{\circ}C$, 48 hours), 1% loess-sulfur only ($30^{\circ}C$, 48 hours) and 1% loess-sulfur only ($20^{\circ}C$, 48 hours) through additional test in greenhouse. Above 3 conditions were verified by rice seedling box and paddy field test in the way of investigating Bakanae diseased plants (%) and healthy plants (%). Consequently, most effective rice seed disinfection conditions on Bakanae disease were combination of hot water and 1% loess-sulfur and loess-sulfur only at $30^{\circ}C$. Furthermore, treatments with these conditions showed control value of 100% were maintained from seedling to the heading stage in the field. However, treatment of 1% loess-sulfur only at $20^{\circ}C$ showed low control value of 78.2% in paddy field. Hot water only treatment turned out to be an effective disinfection method when conducted thoroughly with $60^{\circ}C$, 10 min. However, it was thought additional soaking process with loess-sulfur after hot water treatment served more high control effect against Bakanae disease when rice seeds were disinfected on a large scale. This results expected rice seed disinfection with loess-sulfur were effectively and easily usable method if farmers had only one of either hot water-disinfector or seed-disinfector. In addition, loess-sulfur is well-known to farmers, simple to manufacture method and cheap.

Development of New Organic Filler Made from Rice Husk by Paperboard Mill Trials (산업용지 현장테스트를 통한 왕겨 유기충전제 최적 사양 탐색)

  • Lee, Ji Young;Kim, Young Hun;Kim, Chul Hwan;Sung, Yong Joo;Wi, Sang Wook;Park, Jong-Hea;Kim, Eun Hea
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.47 no.4
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    • pp.96-101
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    • 2015
  • In the previous study, we investigated the physical properties of new organic fillers made from major agricultural byproducts, including rice husks, peanut husks and garlic stems, and we estimated that rice husk was the best candidate for use as new organic fillers in paperboard. In this study, an organic filler prototype was produced with rice husk and the mill trials were carried out in a white liner chipboard (duplexboard) mill. The rice husk organic filler was added to the middle ply of SC $350g/m^2$ to determine the optimal conditions for the manufacture of rice husk organic fillers. The mill trials were performed three times and the bulk improvement and drying energy reduction were measured to identify the functionality of the rice husk organic filler compared to that of the commercial wood powder. In the first mill trial, the test failed because the surface roughness of the duplexboard had deteriorated after the rice husk organic filler was added to the OCC stock. As all of the particles remaining on the 60 mesh sieves were removed and the particle size was decreased by increasing the length of the grinding process, the surface roughness of the duplexboard did not be deteriorated in the second mill trial. However, the bulk improvement and drying energy reduction were not observed. In the final mill trial, as the particle size of the rice husk organic filler was controlled by increasing the portion of particles passing through the 60 mesh sieves and remaining 100 mesh sieves, higher bulk improvement and drying energy reduction were acquired compared to the commercial wood powder.