• Journal of Dairy Science and Biotechnology
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• 제31권2호
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• pp.165-170
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• 2013

Milk and dairy products are not only excellent foods for humans, providing plentiful varied nutrients, but are also a good medium for detrimental food-borne pathogens. Although the food safety field has stabilized due to standardization of food processing, such as the hazard analysis critical control point (HACCP), outbreaks and cases caused by food-borne pathogens still occur at high rates. In approximately 30% of cases, the disease-causing pathogenic organism is undetermined. Recently, a biosensor was developed that has a simple and fast response and overcomes the problems of conventional methods such as cultivation, immuno-assay, polymerase chain reaction, and microarray. Due to the high selectivity and sensitivity of optical biosensors, it is a suitable method for the immediate detection of food-borne pathogens in milk and dairy products.

• Current Optics and Photonics
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• 제4권1호
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• pp.81-81
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• 2020

• 한국광학회지
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• 제30권1호
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• pp.15-22
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• 2019

소산파 기반 집적광학 바이오센서에 적합한 $Si_3N_4$ 립-광도파로 구조에 대한 해석적 분석을 통해서 단일모드 도파조건을 도출하였다. 두-모드 간섭현상을 이용하는 집적광학 바이오센서 구조를 제시하고, 단일모드와 두-모드(감지영역)에 해당되는 립-광도파로 제원인 폭, 립 및 코어 두께 각각에 대해서 $3{\mu}m$, 2 nm, 150 nm와 $3{\mu}m$, 20 nm, 340 nm를 제안하였으며, FMM 전산해석을 통해서 감지영역의 두-모드들에 대한 광학적 특성들을 검토하였다.

• 센서학회지
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• 제27권1호
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• pp.25-30
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• 2018

Closed-form analytical expressions and 3-dimensional normalized charts for the homogeneous sensing and surface sensing structures are derived to provide the conditions for the maximum sensitivity of integrated-optic biosensors based on evanescent-wave and stepindex planar optical waveguides. The analysis is made for transverse electric (TE) polarization mode, in both cases where the measurand is homogeneously distributed in the cover (namely, homogeneous sensing), and where it is an ultrathin film on the waveguide-cover interface (namely, surface sensing).

• Journal of Biosystems Engineering
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• 제32권6호
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• pp.440-447
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• 2007

In this study, a biosensor was developed using an enzyme-linked immunosorbent assay (ELISA) to rapidly measure the fungicide iprovalicarb residues in agricultural products. The biosensor was designed to include micro-pumps and solenoid valves for fluid transport, a spectrophotometer cuvet as a reaction chamber, a photodiode with a light-emitting diode for optical density measurement, and a control microcomputer to implement assay. The rate of change in optical density of the cuvet was read as final signal output. Micro-pumps were evaluated to investigate their delivery capability, the highest values of the error and the coefficient of variation were 4.3% and 4.6% respectively. As the incubation period was reduced from 15 minutes to 11 minutes to shorten the total processing time, the sensor sensitivity was decreased as the antibody dilution ratio was reduced to a half. The maximum usable period of the coated cuvet was found to be two days with 1% error limit. To predict the concentration of the iprovalicarb residue in agricultural products, a linear calibration model was obtained with r-square values of 0.992 for potato and 0.985 for onion. In validation test for the samples of potatoes and onions against the high performance liquid chromatography, very high correlation values were obtained as 0.996 and 0.993 respectively. Using the cuvet immobilized with antigen, it took 21-minutes for the biosensor to complete the measuring process of the iprovalicarb residues.

• 센서학회지
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• 제29권3호
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• pp.172-179
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• 2020

We studied an integrated-optic biosensor configuration that operates at a wavelength of 0.63 ㎛ based on the evanescent-wave and two horizontal mode power coupling of Si₃N₄ rib-optical waveguides formed on a Si/SiO₂/Si₃N₄/SiO₂ multilayer thin films. The sensor consists of a single-mode input waveguide, followed by a two-mode section which acts as the sensing region, and a Y-branch output for separating the two output waveguides. The coupling between the two propagating modes in the sensing region produces a periodically repeated optical power exchanges along the propagation. A light power was steered from one output channel to the other due to the change in the cladding layer (bio-material) refractive index, which affected the effective refractive index (phase-shift) of two modes through evanescent-wave. Waveguide analyses based on the rib optical waveguide dimensions were performed using various numerical computational software. Sensitivity values of 12~23 and 65~165 au/RIU, respectively for the width and length of 4 ㎛, and 3841.46 and 26250 ㎛ of the two-mode region corresponding to the refractive index range 1.36~1.43 and 1.398~1.41, respectively, were obtained.

• Food Science and Biotechnology
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• 제17권5호
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• pp.1038-1046
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• 2008

This study was carried out to develop a small-sized biosensor based on surface plasmon resonance (SPR) for the rapid identification of insecticide residues for food safety. The SPR biosensor module consists of a single 770 nm-light emitting diodes (LED) light source, several optical lenses for transferring light, a hemisphere sensor chip, photo detector, A/D converter, power source, and software for signal processing using a computer. Except for the computer, the size and weight of the sensor module are 150 (L)$\times$70 (W)$\times$120 (H) mm and 828 g, respectively. Validation and application procedures were designed to assess refractive index analysis, affinity properties, sensitivity, linearity, limits of detection, and robustness which includes an analysis of baseline stability and reproducibility of ligand immobilization using carbamate (carbofuran and carbaryl) and organophosphate (cadusafos, ethoprofos, and chlorpyrifos) insecticide residues. With direct binding analysis, insecticide residues were detected at less than the minimum 0.01 ppm and analyzed in less than 100 sec with a good linear relationship. Based on these results, we find that the binding interaction with active target groups in enzymes using the miniaturized SPR biosensor could detect low concentrations which satisfy the maximum residue limits for pesticide tolerance in Korea, Japan, and the USA.

• Journal of Biosystems Engineering
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• 제30권5호
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• pp.306-311
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• 2005

In this study, a biosensor was developed to rapidly diagnose the swine sarcoptic mange (Sarcoptes scabies var. suis). The ELISA was modified to reduce the processing time for rapid diagnosis. The biosensor consists of a biological reaction part, and a measurement and control part. The biological reaction part was designed for using micro-pumps and valves for fluid transportation, and the measurement control part composed of a photodiode, a light-emitting diode fur light measurement, and a microcomputer to implement assay A polystyrene covet was used as a reaction chamber. Signal output was read as the rate of change in optical density at 645nm. Eighteen pigs diagnosed with sacroptic mange and 19 control pigs were tested. Fifteen sacbies-infested pigs showed positive results ($83.3\%$ sensitivity). Sixteen control pigs showed negative results ($84.2\%$ specificity). The system could execute a diagnosis cycle in about 45 min. The results suggest that this biosensor is useful for the rapid diagnosis of swine sacroptic mange.

• 센서학회지
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• 제20권2호
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• pp.107-113
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• 2011

LSPR(Localized Surface Plasmon Resonance) sensor measures the refractive index change on the sensor surface. The detection of biological reaction with the unknown refractive index needs to be converted into the signal sensitivity for the refractive index change for comparison with other measurements. To find the signal sensitivity, the three steps of signal processing are proposed, which are signal modeling, signal calibration and signal normalization of LSPR sensor. The detected signal of biotin-streptavidin interaction has been converted into unit of [RU](Resonance Unit) using the proposed method. The converted signal directly can be compared with the other sensors including commercialized one.

• 한국광학회지
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• 제16권5호
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• pp.433-439
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• 2005

백색 광원과 컬러 센서를 이용하여 바이오 센서의 발색 정도를 측정하는 측정기를 개발하였다. 컬러 센서의 적색, 녹색 및 청색영역의 빛의 세기를 비율로 나타냄으로서, 조사 광의 세기가 변하는 경우에도 안정적인 분광 정보를 얻을 수가 있었고, 바이오 센서의 화학적 처리 과정에서 발생하는 수막 등의 영향에 의한 오차를 감소시킬 수 있다. 바이오 센서의 발색 변화율이 작은 경우 컬러 센서의 각 영역에 대한 증폭률을 조정하는 것만으로 시스템의 민감도를 향상 시킬 수 있었다.