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http://dx.doi.org/10.5307/JBE.2005.30.5.306

A Biosensor for Diagnosis of Swine Sarcoptic Mange  

Cho H. K. (Dept. of Biosystems Engineering, Chungbuk National University)
Jee C. H. (Dept. of Veterinary Medicine, Chungbuk National University)
Publication Information
Journal of Biosystems Engineering / v.30, no.5, 2005 , pp. 306-311 More about this Journal
Abstract
In this study, a biosensor was developed to rapidly diagnose the swine sarcoptic mange (Sarcoptes scabies var. suis). The ELISA was modified to reduce the processing time for rapid diagnosis. The biosensor consists of a biological reaction part, and a measurement and control part. The biological reaction part was designed for using micro-pumps and valves for fluid transportation, and the measurement control part composed of a photodiode, a light-emitting diode fur light measurement, and a microcomputer to implement assay A polystyrene covet was used as a reaction chamber. Signal output was read as the rate of change in optical density at 645nm. Eighteen pigs diagnosed with sacroptic mange and 19 control pigs were tested. Fifteen sacbies-infested pigs showed positive results ($83.3\%$ sensitivity). Sixteen control pigs showed negative results ($84.2\%$ specificity). The system could execute a diagnosis cycle in about 45 min. The results suggest that this biosensor is useful for the rapid diagnosis of swine sacroptic mange.
Keywords
Biosensor; ELISA; Swine sarcoptic mange (Sarcoptes scabies var. suis);
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Times Cited By KSCI : 1  (Citation Analysis)
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