• Journal of Life Science
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• v.29 no.9
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• pp.949-954
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• 2019

Membrane topology is a key characteristic of membrane proteins. We previously reported the cloning of the chromosome 4 open-reading frame 32 (C4orf32) gene as a potential membrane protein; however, the cellular localization and membrane topology of C4orf32 was as yet unknown. In this study, we found that green fluorescent protein (GFP) fused to the C-terminus of C4orf32 (C4orf32-GFP) was localized to the endoplasmic reticulum (ER). We applied three tools to identify determinants of C4orf32 topology: protease protection, fluorescence protease protection (FPP), and an inducible system using the ternary complex between FK506 binding protein 12 (FKBP), rapamycin, and the rapamycin-binding domain of mTOR (FRB) (the FRB-rapamycin-FKBP system). Using protease protection and FPP assays, we found that the GFP tag in C4orf32-GFP was localized to the cytoplasmic surface of the ER membrane of HeLa cells. Protease protection and FPP assays are useful and complimentary tools for identifying the topology of GFP fusion membrane proteins. The FRB-rapamycin-FKBP system was also used to study the topology of C4orf32. In the absence of rapamycin, a monomeric red fluorescent protein-FKBP fusion (mRFP-FKBP) and C4orf32-GFP-FRB were localized to the cytoplasm and the ER membrane, respectively. However, in the presence of rapamycin, the mRFP-FKBP was shifted from the cytoplasm to the ER and colocalized with the C4orf32-GFP-FRB. These results indicate that the FRB moiety is facing the cytoplasmic surface of ER membrane. Overall, our results clearly suggest that C4orf32 belongs to the family of type I ER resident membrane proteins.

• Journal of Digital Convergence
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• v.17 no.1
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• pp.347-356
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• 2019

Recently, the liberalization of industrial control systems has been accompanied by a major change in the structural paradigm of information systems in the public and public sectors, and potential cyber security problems in the future major infrastructure control systems that cannot respond to the level of security of existing information systems. To cope with this, a cyber security evaluation tool that can evaluate security vulnerability in three dimensions against various infrastructure control system environment is needed. However, a cyber security evaluation in the domestic environments does not have the concept of the current security status and satisfy settings of the infrastructure. Also, the most of items in that environments have had short-term inspection themselves which makes a limitation by a technical area. In order to overcome this problems, many researches are needed to apply CSET (Cyber Security Evaluation Tool) which is the US cyber security evaluation tool to the control environment of various domestic infrastructure. In this paper, first, we analyze methods to apply to the major domain through the analysis of various case studies on existing security assessement tools. Finally, we discuss future directions.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.40 no.5
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• pp.429-437
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• 2022

Recently, the demand for a QA (Question Answering) system for human-machine communication has increased. Among the QA systems, a closed domain QA system that can handle spatial-related questions is called GeoQA. In this study, a new type of graph database, LPG (Labeled Property Graph) was used to overcome the limitations of the RDF (Resource Description Framework) based database, which was mainly used in the GeoQA field. In addition, GraphQL (Graph Query Language), an API-type query language, is introduced to address the fact that the LPG query language is not standardized and the GeoQA system may depend on specific products. In this study, database was built so that answers could be retrieved when spatial-related questions were entered. Each data was obtained from the national spatial information portal and local data open service. The spatial relationships between each spatial objects were calculated in advance and stored in edge form. The user's questions were first converted to GraphQL through FOL (First Order Logic) format and delivered to the database through the GraphQL server. The LPG used in the experiment is Neo4j, the graph database that currently has the highest market share, and some of the built-in functions and QGIS were used for spatial calculations. As a result of building the system, it was confirmed that the user's question could be transformed, processed through the Apollo GraphQL server, and an appropriate answer could be obtained from the database.

• Journal of Korean Society of Rural Planning
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• v.29 no.3
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• pp.25-37
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• 2023

According to the 「Rural Spatial Reconstruction and Regeneration Support Act」, enacted on March 29, 2024, all local governments are required to establish a 'Rural Spatial Reconstruction and Regeneration Plan' (hereinafter referred to as the 'Rural Spatial Plan'). In order for the 'Rural Spatial Plan' to be appropriately established, this study analyzed the supply and demand of spatial data from the perspective of user stakeholders and derived implications for improving rural spatial planning data utilization. In conclusion, three key recommendations come from this result. Firstly, it is necessary to establish an integrated DB for rural spatial planning data. This can solve the problem of low awareness of scattered data-providing websites, reduce the processing time of non-GIS data, and reduce the time required to acquire data by securing the availability of data search and download. In particular, research should be conducted on the establishment of a spatial analysis simulation system to support stakeholders' decision-making, considering that many stakeholders have difficulty in spatial analysis because spatial analysis techniques were not actively used in rural projects before the implementation of the rural agreement system in 2020. Secondly, research on how to improve data acquisition should be conducted in each data sector. The data sector group with the lowest ease of receiving are 'Local Community Domain', 'Changes in Domestic and International Conditions', and 'Provision and Utilization of Daily Life Services'. Lastly, in-depth research is needed on how to raise each rural spatial planning data supply stakeholder to the position of player. Stakeholders of 'University Institutions' and 'Public Enterprises and Research Institutes' should give those who participate in the formulation of rural spatial plans access to the raw data collected for public work. Stakeholders of 'Private company' need to come up with realistic measures to build a data pool centered on consultative bodies between existing private companies and then prepare a step-by-step strategy to fully open it by participating various stakeholders. In order to induce 'Village Residents and Associations' stakeholders to play a leading role as owners and producers of data, personnel should be trained to collect and record data related to the village. In addition, support measures should be prepared to continue these activities.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.43 no.4
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• pp.501-510
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• 2023

The importance of using construction data is increasing in accordance with the recent trend in the smart construction. However, construction project and maintenance information is distributed on the web, and the existing BIM(Building Information Modeling) information exchange and linking method using IFC(Industry Foundation Classes) cannot support connection with BIM data and web resources. This study aims to establish the BIM-based port facility data integration system using linked data(LD) technology in order to integrate BIM and heterogeneous data in the port maintenance domain. To this end, the port BIM-based ifcOWL and port facility maintenance ontology were designed, and LD was built for the BIM and maintenance information of Busan New Port 2-1 Pier3, a BIM pilot project. In addition, service prototypes such as search, statistics and SPARQL(SPARQL Protocol and RDF Query Language) endpoint functions were implemented using the issued LD. The LD-based information utilization system is expected to improve the reusability of information by converting the existing closed information system into an open system and BIM and maintenance data as a web resource in a standard format.

• Journal of Navigation and Port Research
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• v.48 no.3
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• pp.155-163
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• 2024

As the demand for reducing carbon emissions increases, efforts to reduce the usage of fossil fuels and research on renewable energy are also increasing. Among the various renewable energy harvesting techniques, the floating offshore wind turbine has several advantages. Compared to other technologies, it has fewer installation limitations due to interference with human activity. Additionally, a large wind turbine farm can be constructed in the open ocean. Therefore, it is important to conduct motion analysis of floating offshore wind turbines in waves during the initial stage of conceptual design. In this study, a frequency motion analysis was conducted on a semi-submersible type floating offshore wind turbine. The analysis focused on the effects of varying trim on the motion characteristics. Specifically, motion response analysis was performed on heave, roll, and pitch. Natural period analysis confirmed that changing the trim angle did not significantly affect the heave and pitch motions, but it did have a regular impact on the roll motion.

• Information Systems Review
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• v.19 no.4
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• pp.27-42
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• 2017

With the rapid development in network, graphic technology, and digital technology, content industry is emerging as an important industry for new cultural development and economic development. The development in digital content technology has remarkably expanded the generation and distribution of contents, thereby creating new value and extending into a large distribution market. However, the ease of distribution and duplication, which characterizes digital technology, has increased the circulation of illegal contents due to illegal copying, theft, and alteration. The damage caused by this illegal content is severe. Currently, a copyright protection system targeting online sites is available. By contrast, no system has been established for offline companies that sell offline genuine content, which compete with online companies. The demand for content of overseas tourists is increasing due to the Korean wave craze. Nevertheless, many offline content providers have lost competitiveness due to illegal content distribution with online companies. In this study, we analyzed the case and status of similar copyright certification systems in Korea and overseas through previous research and studied a system to certify the offline genuine contents business. In addition to the case analysis, we focused on interviews obtained through in-depth interviews with the copyright stakeholders. We also developed a certification framework by establishing the certification domain, certification direction, and incentive of the certification system for offline businesses with genuine content. Selected certification direction is ethical, open, inward, store, and rigid (post evaluation). This study aimed to increase awareness among consumers about the use of genuine content and establish a transparent trading order in a healthy content market.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.10
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• pp.578-589
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• 2018

Building Integrated Photovoltaic (BIPV) system is a typical integrated system that simultaneously performs both building function and solar power generation function. To maximize its potential advantage, however, the solar photovoltaic power generation function must be integrated from the early conceptual design stage, and maximum power generation must be designed. To cope with such requirements, preliminary research on BIPV design process based on architectural design model and computer simulation results for improving solar power generation performance have been published. However, the requirements of the BIPV system have not been clearly identified and systematically reflected in the subsequent design. Moreover, no model has verified the power generation design. To solve these problems, we systematically model the requirements of BIPV system and study power generation design based on the system requirements model. Through the study, we consistently use the standard system modeling language, SysML. Specifically, stakeholder requirements were first identified from stakeholders and related BIPV standards. Then, based on the domain model, the design requirements of the BIPV system were derived at the system level, and the functional and physical architectures of the target system were created based on the system requirements. Finally, the power generation performance of the BIPV system was evaluated through a simulated SysML model (Parametric diagram). If the SysML system model developed herein can be reinforced by reflecting the conditions resulting from building design, it will open an opportunity to study and optimize the power generation in the BIPV system in an integrated fashion.

• Journal of the Daesoon Academy of Sciences
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• v.28
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• pp.37-72
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• 2017

The purpose of this article is to study the prospect of implementing a public common practice of ethics based on the 'Reordering Works of Heaven and Earth.' The 'Reordering Works of Heaven and Earth' demonstrated the prospect of a public common practice of ethics through the shared public action that would connect and mediate both the public domain and the private domain. In addition, the 'Reordering Works of Heaven and Earth' of Gucheon Sangje (九天上帝) provided us with a complete transformation, meaning the opening of a new era, transforming heaven and earth from their state in the Prior World to their optimized state in the Later World. This culminates in a new manifestation of a peaceful world revealed a prosperity and the prospect of common happiness and common order for the public. In addition, the 'Reordering Works of Heaven and Earth' has a public value as a religious culture that responds to social change and social needs. The 'Reordering Works of Heaven and Earth' revealed the prospect of a public common practice of ethics, placed importance on creating a new foundation, and restoring phenomena back to its original order. The 'Reordering Works of Heaven and Earth' presented various multi-faceted, multi-layered outlets of soteriology while exploring solutions to public issues and revealing human dignity. Through the new construction of Heaven and Earth, Gucheon Sangje had intervened in projects for human relief. The public ethical indicators of 'the actor' here are in line with those of the 'Non-action Tao' of Laozi. As the cosmos enters into the new epoch, humans have living together with the previous cosmic principle. Now we can expect the Prior World to open into a different era as humans embrace a cosmic life of 'Non-action Tao.' The active conjunction of the virtues of yin and yang is an idea of horizontal communication related to reordering of the universe. The harmonious union of divine beings and human beings suggests the way of enabling vertical communication. The resolution of grievances for the mutual beneficence of life is an ethics of peace that aims at achieving coexistence and prosperity. The private realization of Tao and the completion of the Tao in the world suggest the prospect of a common practice of ethics as means of implementing human dignity.

• Korean Journal of Soil Science and Fertilizer
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• v.36 no.3
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• pp.134-144
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• 2003

A rhizobacterium Pseudomonas cholororaphis O6 produced several secondary metabolites, such as phenazines, protease, and HCN that may be involved in inhibition of the growth of phytopathogenic fungi. In field study, P. chlororaphis O6 treatment on wheat seed suppressed root rot disease caused by Fusarium culmorum. The major organic acids of cucumber root exudates were fumaric acid, malic acid, benzoic acid, and succinic acid. Glucose and fructose were major monosaccharides in cucumber root exudates. The total amount of organic acids was ten times higher than that of the sugars. P. chlororaphis O6 grew well on cucumber root exudates. The dctA gene of P. chlororaphis O6 consisted of a 1,335 bp open reading frame with a deduced amino acid sequence of 444 residues, corresponding to a molecular size of about 47 kD and pI 8.2. The deduced dctA sequence has ten putative transmembrane domains, as expected of a membrane-embedded protein. Our results indicated that organic acids in cucumber root exudates may play an important role in providing nutrient source for root colonization of biological control bacteria, and the dctA gene of P. chlororaphis O6 may be an important bacterial trait that is involved in utilization of root exudates.