• Title/Summary/Keyword: Open A. I.

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Molecular Characterization of Plasmid from Bifidobacterium longum

  • Park, Myeong-Soo;Moon, Hye-Won;Ji, Geun-Eog
    • Journal of Microbiology and Biotechnology
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    • v.13 no.3
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    • pp.457-462
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    • 2003
  • The complete nucleotide sequence of a plasmid, pMG1, isolated from Bifidobacterium longum MG1 has been determined. This plasmid, composed of 3,862 base pairs with 65.1% of G+C content. harbors two major open reading frames (ORF) encoding putative proteins of 29 kDa (ORF I) and 71 kDa (ORF II). ORF I showed relatively high amino acid sequence homology with replication proteins of other plasmids from Gr Im-positive and -negative bacteria. Upstream of ORF I, four sets of tandem repeat sequences resembling the iteron structure of related plasmids were found. S1 endonuclease treatment and Southern blot analysis revealed that pMG1 accumulates single-stranded DNA (ssDNA) intermediate, which indicate i the rolling circle replication (RCR) mechanism of this plasmid. Homology search indicated that ORF II encodes plasmid mobilization protein, and the presence of highly conserved oriT sequence in the upstream of this gene supported this assumption. RT-PCR showed that only ORF I is expressed in vivo. Based on these results, pMG 1 was exploited to construct a shuttle vector, pBES2. It was successfully transformed into Bifidobacterium and maintained stably.

A Comparative Analysis on Elementary Students방 Scientific Attitudes by Questionnaire and Ethnographic Study (질문지법과 문화기술적 방법에 의한 초등학생의 과학적 태도에 대한 비교 분석)

  • 김운배;김효남
    • Journal of Korean Elementary Science Education
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    • v.19 no.1
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    • pp.1-13
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    • 2000
  • For a profound understanding about the elementary students' scientific attitude, I have performed this study based on the students' opinion by the ethnographic interview and participant observation of science classes. I have analyzed the data by qualitative data analyzing method, and compared the analyzed results with analyzed results by the Likert's scaled as il questionnaire method. To perform this study, I selected one elementary school located in an agricultural village in Choong-Buk Province in Korea and studied four 5th grade students. Here, I present results of this study as follows. First, in ethnographic interview, all informants represented positive reaction to scientific curiosity. But, It is appeared that there are external curiosity and internal curiosity. Internal curiosity is more closely and positively related to problem solving skills. Second, all informants represented positive open-mindness expressing their own opinions actively and accepting other students' opinions. Students who had positive critical-mindness and voluntariness recorded high scores in science achievement. Third, scientific attitude analyzed by ethnographic methods didn't correspond with scientific attitude measured by Likert's scale in all cases. I presumed this discrepant result could come from subjects' different tendency on replying to questionnaire and students' own criterion.

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Changes of Soyasaponin Contents in Soybean Sprouts (콩나물 생육기간 중 사포닌 함량의 변화)

  • Chang, Seo-Young;Han, Sangjun
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.61 no.1
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    • pp.57-63
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    • 2016
  • Soyasaponin I, II, III and V contents were investigated in seed, cotyledons and sprouts of soybean (Glycine max (L.) Merill) subjected to germination over five days. High-performance liquid chromatography coupled with tandem mass spectrometric (HPLC-MS/MS) method was used to evaluate the content of soyasaponins. Soyasaponins contents were different according to the varieties. Germination of soybeans dramatically increased soyasaponin contents in soybean sprouts in a time-dependent manner. Cotyledons had a higher contents of soyasaponins compared to dried seed (p<0.05). After five days of germination, Soyasaponin I and II increased 10 times higher after germination. Soyasaponin I and II are major metabolites in cotyledons and hypocotyls. Soyasaponin III and V were also detected in seed and increased depended on the germination stage. Soyasaponin V was at its highest levels in the hypocotyl, almost 7 times higher than the initial content in soybean seeds. Therefore, the germination of soybean sprouts significantly increased soyasaponin content.

Modeling of Hanlim's gas turbine generator & qualitative analysis of PSS operation (한림가스터빈 발전기/제어계의 모델링 및 PSS 동작의 정성적 분석)

  • Choi, K.S.;Moon, Y.H.;Kim, D.J.;Choo, J.B.;Lyu, S.H.
    • Proceedings of the KIEE Conference
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    • 1996.11a
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    • pp.95-98
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    • 1996
  • Response speed of generator/control system kas increased with the aid of the development of power electronics. Even though it is desirable to enhance response speed for the control system(AVR/Gov) of generator itself, in case a certain generator/control system with high response excitation system is connected with bulk power system, terminal voltage and active power of some generators can oscillate with adjoining generators or near area when even a little of disturbance take place. PSS(Power System Stabilizer) is used to damp rotor swing by adding the supplementary signal in phase with speed. As the stable AVR response is very important before PSS is installed, modeling and analysis of generator/control system was performed. Next we have analysed PSS response of Hanlim's gas turbine by transmission line open/close test.

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Mathematical Consideration on PV Cell Modeling (PV cell modeling의 수학적 고찰)

  • Park, Hyeonah;Kim, Hyosung
    • The Transactions of the Korean Institute of Power Electronics
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    • v.19 no.1
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    • pp.51-56
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    • 2014
  • PV cell modeling is necessary both for software and hardware simulators in analyzing and testing the performance of PV generation systems. Unique I-V curve of a PV cell identifies its own characteristics by electrical equivalent model that is composed of diode constants ($I_o$, $v_t$), photo-generated current ($I_{ph}$), series resistance ($R_s$), and shunt resistance ($R_{sh}$). Photo-generated current can be easily estimated since it is proportional to irradiation level. However, other electrical parameters should be solved from the manufacturer's data sheet that is consisted with three remarkable operating points such as open circuit voltage ($V_{oc}$), short circuit current ($I_{sc}$), and maximum power voltage/current ($V_{MPP}/I_{MPP}$). This paper explains and analyzes mathematical process of a novel PV cell modeling algorithm that was proposed by the authors with the name of "K-algorithm".

Design and Implementation of a Six-Stage Pipeline RV32I Processor Based on RISC-V Architecture (RISC-V 아키텍처 기반 6단계 파이프라인 RV32I프로세서의 설계 및 구현)

  • Kyoungjin Min;Seojin Choi;Yubeen Hwang;Sunhee Kim
    • Journal of the Semiconductor & Display Technology
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    • v.23 no.2
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    • pp.76-81
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    • 2024
  • UC Berkeley developed RISC-V, which is an open-source Instruction Set Architecture. This paper proposes a 32-bit 6-stage pipeline architecture based on the RV32I RSIC-V. The performance of the proposed 6-stage pipeline architecture is compared with the existing 32-bit 5-stage pipeline architecture also based on the RV32I processor ISA to determine the impact of the number of pipeline stages on performance. The RISC-V processor is designed in Verilog-HDL and implemented using Quartus Prime 20.1. To compare performance the Dhrystone benchmark is used. Subsequently, peripherals such as GPIO, TIMER, and UART are connected to verify operation through an FPGA. The maximum clock frequency for the 5-stage pipeline processor is 42.02 MHz, while for the 6-stage pipeline processor, it was 49.9MHz, representing an 18.75% increase.

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PRIMARY IDEALS IN THE RING OF COTINUOUS FUNCTIONS

  • Bae, Soon Sook
    • Kyungpook Mathematical Journal
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    • v.18 no.1
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    • pp.105-107
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    • 1978
  • Considering the prime z-filters on a topological space X through the structures of the ring C(X) of continuous functions. a prime z-filter is uniquely determined by a primary z-ideal in the ring C(X), i. e., they have a one-to-one correspondence. Any primary ideal is contained in a unique maximal ideal in C(X). Denoting $\mathfrak{F}(X)$, $\mathfrak{Q}(X)$, 𝔐(X) the prime, primary-z, maximal spectra, respectively, $\mathfrak{Q}(X)$ is neither an open nor a closed subspace of $\mathfrak{F}(X)$.

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Deriving adoption strategies of deep learning open source framework through case studies (딥러닝 오픈소스 프레임워크의 사례연구를 통한 도입 전략 도출)

  • Choi, Eunjoo;Lee, Junyeong;Han, Ingoo
    • Journal of Intelligence and Information Systems
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    • v.26 no.4
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    • pp.27-65
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    • 2020
  • Many companies on information and communication technology make public their own developed AI technology, for example, Google's TensorFlow, Facebook's PyTorch, Microsoft's CNTK. By releasing deep learning open source software to the public, the relationship with the developer community and the artificial intelligence (AI) ecosystem can be strengthened, and users can perform experiment, implementation and improvement of it. Accordingly, the field of machine learning is growing rapidly, and developers are using and reproducing various learning algorithms in each field. Although various analysis of open source software has been made, there is a lack of studies to help develop or use deep learning open source software in the industry. This study thus attempts to derive a strategy for adopting the framework through case studies of a deep learning open source framework. Based on the technology-organization-environment (TOE) framework and literature review related to the adoption of open source software, we employed the case study framework that includes technological factors as perceived relative advantage, perceived compatibility, perceived complexity, and perceived trialability, organizational factors as management support and knowledge & expertise, and environmental factors as availability of technology skills and services, and platform long term viability. We conducted a case study analysis of three companies' adoption cases (two cases of success and one case of failure) and revealed that seven out of eight TOE factors and several factors regarding company, team and resource are significant for the adoption of deep learning open source framework. By organizing the case study analysis results, we provided five important success factors for adopting deep learning framework: the knowledge and expertise of developers in the team, hardware (GPU) environment, data enterprise cooperation system, deep learning framework platform, deep learning framework work tool service. In order for an organization to successfully adopt a deep learning open source framework, at the stage of using the framework, first, the hardware (GPU) environment for AI R&D group must support the knowledge and expertise of the developers in the team. Second, it is necessary to support the use of deep learning frameworks by research developers through collecting and managing data inside and outside the company with a data enterprise cooperation system. Third, deep learning research expertise must be supplemented through cooperation with researchers from academic institutions such as universities and research institutes. Satisfying three procedures in the stage of using the deep learning framework, companies will increase the number of deep learning research developers, the ability to use the deep learning framework, and the support of GPU resource. In the proliferation stage of the deep learning framework, fourth, a company makes the deep learning framework platform that improves the research efficiency and effectiveness of the developers, for example, the optimization of the hardware (GPU) environment automatically. Fifth, the deep learning framework tool service team complements the developers' expertise through sharing the information of the external deep learning open source framework community to the in-house community and activating developer retraining and seminars. To implement the identified five success factors, a step-by-step enterprise procedure for adoption of the deep learning framework was proposed: defining the project problem, confirming whether the deep learning methodology is the right method, confirming whether the deep learning framework is the right tool, using the deep learning framework by the enterprise, spreading the framework of the enterprise. The first three steps (i.e. defining the project problem, confirming whether the deep learning methodology is the right method, and confirming whether the deep learning framework is the right tool) are pre-considerations to adopt a deep learning open source framework. After the three pre-considerations steps are clear, next two steps (i.e. using the deep learning framework by the enterprise and spreading the framework of the enterprise) can be processed. In the fourth step, the knowledge and expertise of developers in the team are important in addition to hardware (GPU) environment and data enterprise cooperation system. In final step, five important factors are realized for a successful adoption of the deep learning open source framework. This study provides strategic implications for companies adopting or using deep learning framework according to the needs of each industry and business.

Identification and Functional Characterization of an afsR Homolog Regulatory Gene from Streptomyces venezuelae ATCC 15439

  • Maharjan, Sushila;Oh, Tae-Jin;Lee, Hei-Chan;Sohng, Jae-Kyung
    • Journal of Microbiology and Biotechnology
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    • v.19 no.2
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    • pp.121-127
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    • 2009
  • Sequencing analysis of a 5-kb DNA fragment from Streptomyces venezuelae ATCC 15439 revealed the presence of one 3.1-kb open reading frame(ORF), designated as afsR-sv. The deduced product of afsR-sv(1,056 aa) was found to have high homology with the global regulatory protein AfsR. Homology-based analysis showed that aftR-sv represents a transcriptional activator belonging to the Streptomyces antibiotic regulatory protein(SARP) family that includes an N-terminal SARP domain containing a bacterial transcriptional activation domain(BTAD), an NB-ARC domain, and a C-terminal tetratricopeptide repeat domain. Gene expression analysis by reverse transcriptase PCR(RT-PCR) demonstrated the activation of transcription of genes belonging to pikromycin production, when aftR-sv was overexpressed in S. venezuelae. Heterologous expression of the aftR-sv in different Streptomyces strains resulted in increased production of the respective antibiotics, suggesting that afsR-sv is a positive regulator of antibiotics biosynthesis.

A Method of Selecting Open Source Software by IFCS (IFCS에 의한 오픈소스 소프트웨어 선정기법)

  • Jeon, Hye-Young;Jin, Jing-Shu;Rhew, Sung-Yul
    • The KIPS Transactions:PartD
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    • v.17D no.2
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    • pp.119-128
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    • 2010
  • The application field of OSS is being expanded farther and the existing studies are well established theoretically but not actually widely used. Because so many criteria and complexity of selection methods result in low efficiency and not proper for use. In this master's thesis, to solve these problems I propose a selecting technique for OSS with less criteria, which is more effective and easy to use than before. Based on the existing studies, we identify assessment criteria which common used and redefine 14 evaluation attributes as well as 31 assessment criteria through elimination and grouping. And evaluate the criteria in four steps such as Initial assessment, Functional assessment, Characteristical assessment and Supplemental assessment. To evaluate the adequacy of suggested technique, 29 database management tools for MySQL are selected as candidates, and applied to the technique I proposed. As the result, time and effort for evaluation is reduced sharply and we can use this technique more easily.