• Nano
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• v.13 no.12
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• pp.1850147.1-1850147.14
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• 2018

In this work, water-soluble and blue-emitting carbon nanodots (CDs) were synthesized from apple peels for the first time via one-step hydrothermal method. The synthetic route is facile, green, economical and viable. The as-prepared CDs were characterized thoroughly by transmission electron microscopy (TEM), X-ray diffraction (XRD), Raman, Fourier transform infrared (FT-IR), X-ray photoelectron (XPS), fluorescence and UV-Vis absorption spectroscopy in terms of their morphology, surface functional groups and optical properties. The results show that these CDs possessed ultrasmall size, good dispersivity, and high tolerance to pH, ionic strength and continuous UV irradiation. Significantly, the CDs had fast and reversible response towards temperature, and the accurate linear relationship between fluorescence intensity and temperature was used to design a novel nanothermometer in a broad temperature range from 5 to $65^{\circ}C$ facilely. In addition, the fluorescence intensity of CDs was observed to be quenched immediately by Cr(VI) ions based on the inner filter effect. A low-cost Cr(VI) ions sensor was proposed employing CDs as fluorescent probe, and it displayed a wide linear range from 0.5 to $200{\mu}M$ with a detection limit of $0.73{\mu}M$. The practicability of the developed Cr(VI) sensor for real water sample assay was also validated with satisfactory recoveries.

• The Plant Pathology Journal
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• v.37 no.6
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• pp.555-565
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• 2021

Bacteriophages infecting Acidovorax citrulli, the causal agent of bacterial fruit blotch, have been proven to be effective for the prevention and control of this disease. However, the occurrence of bacteriophage-resistant bacteria is one of hurdles in phage biocontrol and the understanding of phage resistance in this bacterium is an essential step. In this study, we aim to investigate possible phage resistance of A. citrulli and relationship between phage resistance and pathogenicity, and to isolate and characterize the genes involved in these phenomena. A phage-resistant and less-virulent mutant named as AC-17-G1 was isolated among 3,264 A. citrulli Tn5 mutants through serial spot assays and plaque assays followed by pathogenicity test using seed coating method. The mutant has the integrated Tn5 in the middle of a cupin protein gene. This mutant recovered its pathogenicity and phage sensitivity by complementation with corresponding wild-type gene. Site-directed mutation of this gene from wild-type by CRISPR/Cas9 system resulted in the loss of pathogenicity and acquisition of phage resistance. The growth of AC-17-G1 in King's B medium was much less than the wild-type, but the growth turned into normal in the medium supplemented with D-mannose 6-phosphate or D-fructose 6-phosphate indicating the cupin protein functions as a phosphomannos isomerase. Sodium dodecyl sulfa analysis of lipopolysaccharide (LPS) extracted from the mutant was smaller than that from wild-type. All these data suggest that the cupin protein is a phosphomannos isomerase involved in LPS synthesis, and LPS is an important determinant of pathogenicity and phage susceptibility of A. citrulli.

• BMB Reports
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• v.39 no.3
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• pp.329-334
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• 2006

RNAi (RNA interference) has become a popular means of knocking down a specific gene in vivo. The most common approach involves the use of chemically synthesized short interfering RNAs (siRNAs), which are relatively easy and fast to use, but which are costly and have only transient effects. These limitations can be overcome by using short hairpin RNA (shRNA) expression vectors. However, current methods of generating shRNA expression vectors require either the synthesis of long (50-70 nt) costly oligonucleotides or multi-step processes. To overcome this drawback, we have developed a one-step short-oligonucleotides-based method with preparation costs of only 15% of those of the conventional methods used to obtain essentially the same DNA fragment encoding shRNA. Sequences containing 19 bases homologous to target genes were synthesized as 17- and 31-nt DNA oligonucleotides and used to construct shRNA expression vectors. Using these plasmids, we were able to effectively silence target genes. Because our method relies on the onestep ligation of short oligonucleotides, it is simple, less error-prone, and economical.

• Journal of KIISE:Computer Systems and Theory
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• v.26 no.12
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• pp.1539-1547
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• 1999

캐리-세이브 가산기 (CSA)는 회로 설계 과정에서 빠른 연산 수행을 위해 가장 널리 이용되는 연산기 중의 하나이다. 그러나, 현재까지 산업체에서 CSA를 이용한 설계는 설계자의 경험에 따른 수작업에 의존하고 있고 그 결과 최적의 회로를 만들기 위해 매우 많은 시간과 노력이 소비되고 있다. 이에 따라 최근 CSA를 기초로 하는 회로 합성 자동화 기법에 대한 연구의 필요성이 대두되고 있는 상황에서, 본 논문은 연산 속도를 최적화하는 효율적인 CSA 할당 알고리즘을 제안한다. 우리는 CSA 할당 문제를 2단계로 접근한다: (1) 연산식의 멀티 비트 입력들만을 고려하여 최소 수행 속도 (optimal-delay)의 CSA 트리를 할당한다; (2) (1)에서 구한 CSA 트리의 수행 속도 증가가 최소화 (minimal increase of delay) 되는 방향으로 CSA들의 캐리 입력 포트들에 나머지 싱글 비트 입력들을 배정한다. 실제 실험에서 우리의 제안된 알고리즘을 적용하여 연산식들의 회로 속도를 회로 면적의 증가 없이 상당한 수준까지 줄일 수 있었다.Abstract Carry-save-adder (CSA) is one of the most widely used implementations for fast arithmetics in industry. However, optimizing arithmetic circuits using CSAs is mostly carried out by the designer manually based on his/her design experience, which is a very time-consuming and error-prone task. To overcome this limitation, in this paper we propose an effective synthesis algorithm for solving the problem of finding an allocation of CSAs with a minimal timing for an arithmetic expression. Specifically, we propose a two step approach: (1) allocating a delay-optimal CSA tree for the multi-bit inputs of the arithmetic expression and (2) determining the assignment of the single-bit inputs to carry inputs of the CSAs which leads to a minimal increase of delay of the CSA tree obtained in step (1). For a number of arithmetic expressions, we found that our approach is very effective, reducing the timing of the circuits significantly without increasing the circuit area.

• Textile Coloration and Finishing
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• v.28 no.1
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• pp.40-47
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• 2016

As a coating material for loudspeaker dampers, resilient polyurethane/epoxy hybrid resins were synthesized to replace conventional phenol resin and examined the physical properties, which are not only environmentally friendly but also not harmful to human. Five types of polyurethane resins were synthesized in the step-shot method using methylene diisocyanate, three polyols such as poly tetramethylene ether glycol(PTMEG, MW:2000), poly(1,4-buthylene adipate(PBAP, MW:2000), and poly carbonatediol(PCD, MW:2000), and three chain extenders such as ethylene glycol(EG), neopentyl glycol(NPG), and 1,4-buthandiol(1,4-BD). The five types of synthesized polyurethane resins and commercially available bisphenol A type epoxy resin were blended in weight ratios of 90:10, 70:30, and 50:50 to synthesize 15 types of polyurethane/epoxy hybrid resins. Among the polyurethane resins, the one that was synthesized using PCD and 1,4-BD showed excellent tensile strength, 100% modulus, low extension, and relatively high viscosity. Polyurethane/epoxy hybrid resins with higher epoxy resin contents showed better thermal properties and water resistance while those with higher polyurethane contents showed higher flexibility. The polyurethane/epoxy hybrid resin made by blending the polyurethane based on PCD and 1,4-BD with a bisphenol A type epoxy resin in a weight ratio of 70:30 was identified to be the most suitable to be used in speaker dampers.

• The KIPS Transactions:PartB
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• v.10B no.7
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• pp.803-810
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• 2003

In this paper, we describe a fast image mosaic method for constructing a large-scale image with video image captured from cameras that are arranged in radial shape. In the first step, we adopt the phase correlation algorithm to estimate the horizontal and vertical displacement between two adjacent images. Secondly, we calculate the accurate transform matrix among those cameras with Levenberg-Marquardt method. In the last step, those images are stitched into one large scale image in real-time by applying the transform matrix to the texture mapping function of DirectX. The feature of the method is that we do not need to use special hardware devices or write machine-level programs for Implementing a real-time mosaic system since we use conventional graphic APIs (Application Programming Interfaces), DirectX for image synthesis process.

• The Journal of the Acoustical Society of Korea
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• v.15 no.2
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• pp.72-78
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• 1996

This thesis deals with a method to solve a two-and-one-half-dimensional ($2\frac12$ D) problem, which means that the ocean environment is two-dimensional whereas the source is fully three-dimensionally propagating, including three-dimensional refraction phenomena and three-dimensional back-scattering, using two-dimensional two-way parabolic equation method combined with Fourier synthesis. Two dimensional two-way parabolic equation method uses Galerkin's method for depth and Crank-Nicolson method and alternating direction for range and provides a solution available to range-dependent problem with wave-field back-scattered from discontinuous interface. Since wavenumber, k, is the function of depth and vertical or horizontal range, we can reduce a dimension of three-dimensional Helmholtz equation by Fourier transforming in the range direction. Thus transformed two-dimensional Helmholtz equation is solved through two-way parabolic equation method. Finally, we can have the $2\frac12$ D solution by inverse Fourier transformation of the spectral solution gained from in the last step. Numerical simulation has been carried out for a canonical ocean environment with stair-step bottom in order to test its accuracy using the present analysis. With this spectral parabolic equation method, we have examined three-dimensional acoustic propagation properties in a specified site in the Korean Straits.

• Journal of the Korean Chemical Society
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• v.38 no.2
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• pp.136-145
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• 1994

Polymeric complexes such as M(Ⅱ)(PVPS)(SND), M(Ⅱ)(PVPS)(SOPD) have been prepared with monomeric complexes, M(Ⅱ)(SND) and M(Ⅱ)(SOPD)[M: Co(Ⅱ), Ni(Ⅱ), and Cu(Ⅱ)] and polymer PVPS. These complexes have been indentified by elemental analysis, spectroscopy, and T.G.A. From the results, it was found that M(Ⅱ)(PVPS)(SND), M(Ⅱ)(PVPS)(SOPD) complexes were penta-coordinated configuration. Electrochemical properties of these complexes studied by cyclic voltammetry and differential pulse polarography in 0.1 M TEAP-DMF solution at glassy carbon electrode. Co(Ⅱ)(PVPS)(SND) and Co(Ⅱ)(PVPS)(SOPD) showed irreversible two step reduction, such as Co(Ⅲ)/Co(Ⅱ) and Co(Ⅱ)/Co(Ⅰ), and Ni(Ⅱ)(PVPS)(SND), Ni(Ⅱ)(PVPS)(SOPD), Cu(Ⅱ)(PVPS)(SND), and Cu(Ⅱ)(PVPS)(SOPD) complexes showed irreversible one step reduction, such as Ni(Ⅱ)/Ni(Ⅰ) and Cu(Ⅱ)/Cu(Ⅰ), respectively.

• Journal of the Korean Chemical Society
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• v.35 no.1
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• pp.90-95
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• 1991

A method for the selective syntheses of alkyl (alkoxymethyl)benzoates from halobenzyl halides by two steps in one pot process is described. In the first step, benzyl halide moiety is etherified with alkoxide anion in alcohol by Williamson ether process. In the second step, aryl halide moiety is carbonylated to give alkyl (alkoxymethyl)benzoate with alcohol, Na$_2$CO$_3$, CH$_3$I, and carbon monoxide (1 atm) in the presence of a catalytic amount of Co$_2$(CO)$_8$ in excellent yield.

• International Journal of Control, Automation, and Systems
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• v.2 no.1
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• pp.83-91
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• 2004

The purpose of this paper is to obtain an accurate nonlinear system model to test various control schemes for a motion control system that requires high speed, robustness and accuracy. An industrial sewing machine equipped with a Brushless DC motor is considered. It is modeled by a neural network that is configured as an output-error dynamical system. The identified model is essentially a one step ahead prediction structure in which past inputs and outputs are used to calculate the current output. Using the model, a 2 degree-of-freedom PID controller to compensate the effects of disturbance without degrading tracking performance has been de-signed. In this experiment, it is not preferable for safety reasons to tune the controller online on the actual machinery. Experimental results confirm that the model is a good approximation of sewing machine dynamics and that the proposed control methodology is effective.