• Korean Journal of Oriental Medicine
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• v.14 no.3
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• pp.113-119
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• 2008

In pulse diagnosis, the indentation pressure is one of the most important factors as well as the change of pulse shape and the distribution of pressure via time. But, on the oriental medical doctor's indentation pressure control, the understandings of the neurophysiological meanings and mechanisms have been lacked. So, in this paper, we considered on these issues and then proposed a proper system which can imitate the OMD's indentation pressure control mechanisms. As a result, both tactile information and kinesthetic information were found to be essential to the indentation pressure control so that a system, which can measure both the physical indent pressure and the displacement of an indentation arm, has been proposed. With this proposed system, while the indentation was being controlled through the moving step number of the step motor, the physical indentation pres sure and displacement of the indentation arm were measured. From these measured data, the relationships between the moving step number and both physical indentation pressure and displacement were revealed to have linear characteristics in early phase and to have nonlinear characteristics in latter phase. Additionally, three types of graph were generated whose X axis means the moving step number, the physical indentation pressure and the displacement respectively and Y axis means the pulse pressure. By comparing these graphs, we come to conclude that different concepts on indentation pressure control cause different diagnostic results on floating/sinking degrees for the same subject. Consequently, an indentation system for the pulse diagnosis should be able to provide both the tactile information and kinesthetic information, that is, the physical indentation pressure and the displacement of the indentation arm. In future, the proposed system should be optimized to the pulse diagnosis environment and how to combine the both information for more reliable diagnosis should be studied.

• Proceedings of the Korean Society of Applied Pharmacology
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• 2005.04a
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• pp.5-14
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• 2005

Recently, data from several groups have raised the concept of 'checkpoint' in transcription. As capping of nascent RNA transcript is tightly coupled to RNA polymerase II transcription, we seek to obtain direct evidence that transcripiton checkpoint via capping enzyme functions in this early regulatory step. One of temperature sensitive (ts) alleles of ceg1, a guanylyltransferase subunit of the Saccharomyces cerevisiaecapping enzyme, showed 6-azauracil (6AU) sensitivity at the permissive growth temperature, which is a phenotype that is correlated with a transcription elongational defect. This ts allele, ceg1-63 also has an impaired ability to induce PUR5 in response to a 6AU treatment. However, this cellular and molecular defect is not due to the preferential degradation of the transcript attributed from a lack of guanylyltransferase activity. On the contrary, the data suggests that the guanylyltransferase subunit of the capping enzyme plays a role in transcription elongation. First, in addition to the 6AU sensitivity, ceg1-63is synthetically lethal with elongation defective mutations of the largest subunit of RNA polymerase II. Secondly, it exhibited a lower GAL1 mRNA turn-over after glucoseshut off. Third, it decreased the transcription read through a tandem array of promoter proximal pause sites in an orientation dependent manner. Interestingly, this mutant also showed lower pass through a pause site located further downstream of the promoter. Taken together, these results suggest that the capping enzyme plays the role of an early transcription checkpoint possibly in the step of the reversion of repression by stimulating polymerase to escape from the promoter proximal arrest once RNA becomes appropriately capped.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2017.05a
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• pp.978-983
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• 2017

Ammonium dinitramide(ADN) is currently under inder investigation as a replacement for ammonium perchlorate, both for environmental and toxicological reasons. Another promising application is ADN-based liquid monopropellant as a replacement for hydarzine, which is highly toxic and carcinogenic. Production of ADN is today normally performed via guanylurea dinitramide(GUDN) by reaction with potassium hydroxide to yiela potassium dinitramide(KDN). In a second reaction step, KDN is reacted with ammonium sulfate to give ADN. In our new improved process, ADN is synthesized from GUDN in one single reaction step. The simplified process improves purity, reduces the amount of by-products and allows production of ADN at a potentially lower cost, which is crucial to favour the use of ADN.

• Korean Parent-Child Health Journal
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• v.22 no.1
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• pp.1-9
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• 2019

Purpose: The purpose of this study is to develop educational material for parents of hospitalized preschool children who receive nebulizer therapy treatment. The educational material is aimed at providing information on the correct use of nebulizer. Methods: The development of educational material was based on the ADDIE model and consists of an A4-sized leaflet and a video. Results: The leaflet included basic information, procedures, and cautionary information for nebulizer therapy, as well as frequently asked questions. The one minute and fifty second video is accessed via QR code and provides step-by-step instruction on nebulizer therapy. Conclusion: This study recommends that the educational leaflet and video developed for nebulizer therapy use be provided to parents of preschool children hospitalized in the pediatric ward.

• Journal of Plant Biotechnology
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• v.49 no.3
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• pp.193-206
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• 2022

Astringent persimmon (Diospyros kaki Thunb.) is an important fruit crop in Korea; it possesses significant medicinal potential. However, knowledge regarding the pathogens affecting this crop, particularly, viruses and viroids, is limited. In the present study, reverse transcription-polymerase chain reaction (RT-PCR) and high-throughput transcriptome sequencing (HTS) were used to investigate the viruses and viroids infecting astringent persimmons cultivated in Korea. A one-step multiplex RT-PCR (mRT-PCR) method for the simultaneous detection of the pathogens was developed by designing species-specific primers and selecting the primer pairs via combination and detection limit testing. Seven of the sixteen cultivars tested were found to be infection-free. The RT-PCR and HTS analyses identified two viruses and one viroid in the infected samples (n = 51/100 samples collected from 16 cultivars). The incidence of single infections (n = 39/51) was higher than that of mixed infections (n = 12/51); the infection rate of the Persimmon cryptic virus was the highest (n = 31/39). Comparison of the monoplex and mRT-PCR results using randomly selected samples confirmed the efficiency of mRT-PCR for the identification of pathogens. Collectively, the present study provides useful resources for developing disease-free seedlings; further, the developed mRT-PCR method can be extended to investigate pathogens in other woody plants.

• Bulletin of the Korean Chemical Society
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• v.33 no.8
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• pp.2535-2538
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• 2012

$SnO_2@carbon$ tube-core nanowire was synthesized via a facile self-organized method, which was in situ by one step via Chemical Vapor Deposition. The resulting composite was characterized by scanning electron microscopy, X-ray diffraction and transmission electron microscope. The diameter of the single nanowire is between 5 nm and 60 nm, while the length would be several tens to hundreds of micrometers. Then X-ray diffraction pattern shows that the composition is amorphous carbon and tin dioxide. Transmission electron microscope images indicate that the nanowire consists of two parts, the outer carbon tube and the inner tin dioxide core. Meanwhile, the possible growth mechanism of $SnO_2@carbon$ tube-core nanowire is also discussed.

• Toxicological Research
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• v.29 no.2
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• pp.107-114
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• 2013

Mass spectrometry (MS) is a very powerful instrument that can be used to analyze a wide range of materials such as proteins, peptides, DNA, drugs, and polymers. The process typically involves either chemical or electron (impact) ionization of the analyte. The resulting charged species or fragment is subsequently identified by the detector. Usually, single mass uses source-induced dissociation (SID), whereas mass/mass uses collision-induced dissociation (CID) to analyze the chemical fragmentations Each technique has its own advantages and disadvantages. While CID is most effective for the analysis of pure substances, multiple-step MS is a powerful technique to get structural data. Analysis of veterinary drugs ampicillin, chloramphenicol, ciprofloxacin, and oxytetracycline serves to highlight the slight differences between SID and CID. For example, minor differences were observed between ciprofloxacin and oxytetracycline via SID or CID. However, distinct fragmentation patterns were observed for ampicllin depending on the analysis method. Both SID and CID showed similar fragmentation spectra but different signal intensities for chloramphenicol. There are several factors that can influence the fragmentation spectra, such as the collision energy, major precursor ion, electrospray mode (positive or negative), and sample homogeneity. Therefore, one must select a fragmentation method on an empirical and case-by-case basis.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2001.04a
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• pp.24-27
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• 2001

Nowadays, increasing demand of the customized products has led to an increased usage of rapid prototyping in the product development. However, the acquisition price of a rapid prototyping equipment is still too high that not every body can afford to buy one. To offer a wide access to the users who need physical prototypes, a connection of the rapid prototyping equipment to the Internet is a viable step. It would allow a large group of customers all over the world to use the manufacturing capability of a service provider offering this kind of manufacturing service. To realize how such an e-manufacturing concept can look like, a LOM-type 3D printer developed at KIST has been used as test site and connected to the Internet. A possible user can log on to the server of the equipment and view his STL file and start the building operation from a remote place. To see whether the operation runs properly, a CCD camera is used to transmit the actual state of operation online. The result so far proves the feasibility of rapid prototyping on the Internet as well as an order-adaptive manufacturing system via web.

• Bulletin of the Korean Chemical Society
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• v.33 no.6
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• pp.1934-1938
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• 2012

The reaction mechanism between cyclopropenylidene and formaldehyde has been systematically investigated employing the MP2/6-311+$G^*$ level of theory to better understand the cyclopropenylidene reactivity with carbonyl compound. Geometry optimization, vibrational analysis, and energy property for the involved stationary points on the potential energy surface have been calculated. Energies of all the species are further corrected by the CCSD(T)/6-311+$G^*$ single-point calculations. It was found that one important reaction intermediate (INTa) has been located firstly $via$ a transition state (TSa). After that, the common intermediate (INTb) for the two pathways (1) and (2) has been formed $via$ TSb. At last, two different products possessing three- and four-membered ring characters have been obtained through two possible reaction pathways. In the reaction pathway (1), a three-membered ring alkyne compound has been obtained. As for the reaction pathway (2), it is the formation of the four-membered ring conjugated diene compound. The energy barrier of the ratedetermining step of pathway (1) is lower than that of the pathway (2), and the ultima product of pathway (2) is more stable than that of the pathway (1).

• Publications of The Korean Astronomical Society
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• v.30 no.2
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• pp.371-374
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• 2015

Classical Cepheids (hereafter Cepheids) belong to a class of important variable stars that can be used to determine distances to nearby galaxies via the famous period-luminosity (PL) relations, i.e. the Leavitt Law. In turn, these distances can then be used to calibrate a host of secondary distance indicators located well within the Hubble flow, and ultimately determine the Hubble constant in a manner independent of the Cosmic Microwave Background (CMB) measurements. Some recent progress in determining the Hubble constant to within ~ 3% level via the Cepheid-based distance scale ladder (the SH0ES and the Carnegie Hubble Program) were first summarized in this Proceeding, followed by a brief discussion on the prospect of using ultra-long period Cepheids (ULPC) in future distance scale work. ULPC are those Cepheids with periods longer than 80 days, which seem to follow a different PL relation than their shorter period Cepheids. It has been suggested that ULPC can be used to determine the Hubble constant in "one-step". However, based on the two ULPCs found in M31, it was found that the large dispersion in derived distance moduli leads to a less accurate distance modulus to M31 compared to the classical Cepheids. This finding might raise an alert regarding the use of ULPCs in future distance scale work.