Browse > Article
http://dx.doi.org/10.5010/JPB.2022.49.3.193

A survey of viruses and viroids in astringent persimmon (Diospyros kaki Thunb.) and the development of a one-step multiplex reverse transcription-polymerase chain reaction assay for the identification of pathogens  

Kwon, Boram (School of Applied Biosciences, Kyungpook National University)
Lee, Hong-Kyu (School of Applied Biosciences, Kyungpook National University)
Yang, Hee-Ji (School of Applied Biosciences, Kyungpook National University)
Kim, So-Yeon (School of Applied Biosciences, Kyungpook National University)
Lee, Da-Som (School of Applied Biosciences, Kyungpook National University)
An, ChanHoon (Forest Microbiology Division, National Institute of Forest Science)
Kim, Tae-Dong (Forest Tree Improvement and Biotechnology Division, National Institute of Forest Science)
Park, Chung Youl (Division of Wild Plant Seeds Research, Baekdudaegan National Arboretum)
Lee, Su-Heon (School of Applied Biosciences, Kyungpook National University)
Publication Information
Journal of Plant Biotechnology / v.49, no.3, 2022 , pp. 193-206 More about this Journal
Abstract
Astringent persimmon (Diospyros kaki Thunb.) is an important fruit crop in Korea; it possesses significant medicinal potential. However, knowledge regarding the pathogens affecting this crop, particularly, viruses and viroids, is limited. In the present study, reverse transcription-polymerase chain reaction (RT-PCR) and high-throughput transcriptome sequencing (HTS) were used to investigate the viruses and viroids infecting astringent persimmons cultivated in Korea. A one-step multiplex RT-PCR (mRT-PCR) method for the simultaneous detection of the pathogens was developed by designing species-specific primers and selecting the primer pairs via combination and detection limit testing. Seven of the sixteen cultivars tested were found to be infection-free. The RT-PCR and HTS analyses identified two viruses and one viroid in the infected samples (n = 51/100 samples collected from 16 cultivars). The incidence of single infections (n = 39/51) was higher than that of mixed infections (n = 12/51); the infection rate of the Persimmon cryptic virus was the highest (n = 31/39). Comparison of the monoplex and mRT-PCR results using randomly selected samples confirmed the efficiency of mRT-PCR for the identification of pathogens. Collectively, the present study provides useful resources for developing disease-free seedlings; further, the developed mRT-PCR method can be extended to investigate pathogens in other woody plants.
Keywords
astringent persimmon; Citrus viroid VI; multiplex RT-PCR; Persimmon cryptic virus; Persimmon virus A;
Citations & Related Records
Times Cited By KSCI : 9  (Citation Analysis)
연도 인용수 순위
1 Ito T, Sato A, Suzaki K (2015) An assemblage of divergent variants of a novel putative closterovirus from American persimmon. Virus Genes 51:105-111   DOI
2 Jarvis A, Lane A, Hijmans RJ (2008) The effect of climate change on crop wild relatives. Agric Ecosyst Environ 126:13-23   DOI
3 Jevremovic D, Paunovic SA (2019) First report of persimmon cryptic virus in persimmon in North Macedonia. J Plant Pathol 101:1285-1285   DOI
4 Joung SY, Lee SJ, Sung NJ, Jo JS, Kang SK (1995) The chemical composition of persimmon (Diospyros kaki, Thumb) leaf tea. J Korean Soc Food Nutr 24:720-726 (In Korean)
5 Kajiura M (1946) Persimmon cultivars and their improvement. 2 Breed Hortic 1:175-182
6 Kim CG, Lee SM, Jeong HK, Jang JK, Kim YH, Lee CK (2010) Impacts of climate change on Korean agriculture and its counterstrategies. Korea Rural Economic Institute, Seoul (In Korean)
7 Kim DH, Kim IS, Lee G, Cho IS, Cho KH, Shin IS, Kim SH, Chun JA, Choi IM (2015) Current occurrence of Persimmon viroid and Citrus viroid in persimmon in JellaNam-do and testing for viroid inactivation methods. J Plant Biotechnol 42:43-48 (In Korean)   DOI
8 Kim IH, Kim JY, Nam HJ, Lee KU, Cho DH, Lee YJ (2017) Effect of PE film thickness and storage temperature in MAP deastringency of 'Sanggamdungsi' astringent persimmon. Korean J Food Preserv 24:727-733 (In Korean)   DOI
9 Kim NY, Lee HJ, Kim NK, Oh J, Lee SH, Kim H, Moon JS, Jeong RD (2019) Occurrence pattern of viral infection on pear in Korea and genetic characterization of apple scar skin viroid isolates. Hort Sci Technol 767-778
10 Kim YJ, Kim BK (2005) Effect of dietary persimmon peel powder on physico-chemical properties of pork. Korean J Food Sci Anim Resour 25:39-44 (In Korean)
11 Yoo SK, Kim JM, Park SK, Kang JY, Han HJ, Park HW, Kim CW, Heo HJ (2019) Chemical compositions of different cultivars of astringent persimmon (Diospyros kaki Thunb.) and the effects of maturity. Korean J Food Sci Technol 51:248-257 (In Korean)
12 Tilman D, Clark M (2014) Global diets link environmental sustainability and human health. Nature 515:518-522   DOI
13 Wei X, Liu F, Qiu Z, Shao Y, He Y (2014) Ripeness classification of astringent persimmon using hyperspectral imaging technique. Food Bioprocess Technol 7:1371-1380   DOI
14 Xiao J, Grandillo S, Ahn SN, McCouch SR, Tanksley SD, Li J, Yuan L (1996) Genes from wild rice improve yield. Nature 384:223-224   DOI
15 Yonemori K, Honsho C, Kanzaki S, Ino H, Ikegami A, Kitajima A, Sugiura A, Parfitt DE (2008) Sequence analyses of the ITS regions and the matK gene for determining phylogenetic relationships of Diospyros kaki (persimmon) with other wild Diospyros (Ebenaceae) species. Tree Genet Genomes 4:149-158   DOI
16 Morelli M, Chiumenti M, La Notte P, Minafra A, Martelli GP (2014) First report of Persimmon virus A in Italy. J Plant Pathol 96:610
17 Lee S, Cha JS, Kwon Y, Lee YS, Yoo SE, Kim JH, Kim D (2020) Occurrence status of five apple virus and viroid in Korea. Res Plant Dis 26:95-102 (In Korean)   DOI
18 Telis VRN, Gabas AL, Menegalli FC, Telis-Romero J (2020) Water sorption thermodynamic properties applied to persimmon skin and pulp. Thermochim Acta 343:49-56
19 Mac Kenzie DJ, Mc Lean MA, Mukerji S, Green M (1997) Improved RNA extraction from woody plants for the detection of viral pathogens by reverse transcriptionpolymerase chain reaction. Plant Dis 81:222-226   DOI
20 Morelli M, Arli-Sokmen M (2016) First report of persimmon cryptic virus in Turkey. J Plant Pathol 98:183
21 Mujeeb-Kazi A, Kimber G (1985) The Production, Cytology and Practically of Wide Hybrids in the Triticeae. Cereal Res Commun 111-124
22 Nam M, Lee YH, Park CY, Lee MA, Bae YS, Lim S, Lee JH, Moon JS, Lee SH (2015) Development of multiplex RT-PCR for simultaneous detection of garlic viruses and the incidence of garlic viral disease in garlic genetic resources. Plant Pathol J 31:90-96   DOI
23 Park CY, Min HG, Lee HK, Maharjan R, Yoon Y, Lee SH (2018a) Development of a Multiplex Reverse Transcription-Polymerase Chain Reaction Assay for the Simultaneous Detection of Three Viruses in Leguminous Plants. Res. Plant Dis 24:348-352   DOI
24 Seo DH, Jung KM, Kim SJ, Kim KM (2013) Development of EST-SSR markers and analysis of genetic diversity using persimmon (Diospyros kaki Thunb) cultivars collecting from domestic. Korean J Plant Resour 26:491-502 (In Korean)   DOI
25 Suzuki T, Someya S, Hu F, Tanokura M (2005) Comparative study of catechin compositions in five Japanese persimmons (Diospyros kaki). Food Chem 93:149-152   DOI
26 Ito T, Sato A (2020) Three novel viruses detected from Japanese persimmon 'Reigyoku' associated with graft-transmissible stunt. Eur J. Plant Pathol 158:163-175   DOI
27 Asano S, Matsushita Y, Hirayama Y, Naka T (2015) Simultaneous detection of Tomato spotted wilt virus, Dahlia mosaic virus and Chrysanthemum stunt viroid by multiplex RT-PCR in dahlias and their distribution in Japanese dahlias. Lett Appl Microbiol 61:113-120   DOI
28 Cho IS, Yang CY, Yoon JY, Kwon TR, Hammond J, Lim HS (2021) First Report of Passiflora Latent Virus Infecting Persimmon (Diospyros kaki) in Korea. Plant Dis 105:1236
29 George AP, Mowat AD, Collins RJ, Morley-Bunker M (1997) The pattern and control of reproductive development in nonastringent persimmon (Diospyros kaki L.): A review. Sc i Hortic 70:93-122   DOI
30 Hajjar R, Hodgkin T (2007) The use of wild relatives in crop improvement: a survey of developments over the last 20 years. Euphytica 156:1-13   DOI
31 Park CY, Park J, Lee G, Yi SI, Kim BH, Eom JS, Lee SG, Kim H (2018b) Development of a Multiplex RT-PCR for the Simultaneous Detection of Three Viruses in Cherry Plants. Res Plant Dis 24:233-236   DOI
32 Kim ID, Dhungana SK, Chae YG, Son NK, Shin DH (2016) Quality characteristics of 'Dongchul'persimmon (Diospyros kaki Thunb.) fruit grown in Gangwondo, Korea. Korean J Plant Resour 29:313-321 (In Korean)   DOI
33 Kim SY, Jeong SM, Kim SJ, Jeon KI, Park E, Park HR, Lee SC (2006) Effect of heat treatment on the antioxidative and antigenotoxic activity of extracts from persimmon (Diospyros kaki L.) peel. Biosci Biotechnol Biochem 70:999-1002   DOI
34 Lim TH, Choi YH, Song IK, Kim KR, Lee DW, Lee SM (2008) Survey of actual condition of management of persimmon orchards in Sangju, Gyeongbuk in 2007 and 2008. Korean J Pestic Sci 12:414-420 (In Korean)
35 Morelli M, Chiumenti M, De Stradis A, La Notte P, Minafra A (2015) Discovery and molecular characterization of a new cryptovirus dsRNA genome from Japanese persimmon through conventional cloning and high-throughput sequencing. Virus Genes 50:160-164   DOI
36 Nakaune R, Nakano M (2008) Identification of a new Apscaviroid from Japanese persimmon. Arch Virol 153:969-972   DOI
37 Suzuki Y, Hibino T, Kawazu T, Wada T, Kihara T, Koyama H (2003) Extraction of total RNA from leaves of Eucalyptus and other woody and herbaceous plants using sodium isoascorbate. Biotechniques 34:988-993   DOI
38 Tilman D, Balzer C, Hill J, Befort BL (2011) Global food demand and the sustainable intensification of agriculture. Proc Natl Acad Sci 108:20260-20264   DOI
39 Yao B, Wang G, Ma X, Liu W, Tang H, Zhu H, Hong N (2014) Simultaneous detection and differentiation of three viruses in pear plants by a multiplex RT-PCR. J Virol Methods 196:113-119   DOI
40 Cho IS, Park MJ, Kwon SJ, Choi GS, Hammond J, Lim HS (2016) First report of Persimmon cryptic virus and Persimmon virus A in Korea. J Plant Pathol 98:694
41 Gur A, Zamir D (2004) Unused natural variation can lift yield barriers in plant breeding. PLoS Biol 2:e245   DOI
42 Hong JS, Chae KY (2005) Physicochemical characteristics and antioxidant activity of astringent persimmon concentrate by boiling. Korean J Food Cook Sci 21:709-716 (In Korean)
43 Khoury CK, Bjorkman AD, Dempewolf H, Ramirez-Villegas J, Guarino L, Jarvis A, Rieseberg LH, Struik PC (2014) Increasing homogeneity in global food supplies and the implications for food security. Proc Natl Acad Sci 111: 4001-4006   DOI
44 Hwang JH, Park YO, Kim SC, Lee YJ, Kang JS, Choi YW, Son BG, Park YH (2010) Evaluation of genetic diversity among persimmon cultivars (Diospyros kaki Thunb.) using microsatellite markers. J Life Sci 20:632-638 (In Korean)   DOI
45 Ito T, Suzaki K, Nakano M (2013) Genetic characterization of novel putative rhabdovirus and dsRNA virus from Japanese persimmon. J Gen Virol 94:1917-1921   DOI
46 Jing Z, Ruan X, Wang R, Yang Y (2013) Genetic diversity and relationships between and within persimmon (Diospyros L.) wild species and cultivated varieties by SRAP markers. Plant Syst Evol 299:1485-1492   DOI
47 Yu HB, Lim TH, Jung MG, Kim JE, Park CJ, Song JH, Lee DW (2017) Survey on actual pest management condition of persimmon orchards in sancheong, gyeongsangnam-do and yeongdong, chungcheongbuk-do. Korean J Pestic Sci 21: 417-426 (In Korean)   DOI