• 한국가축번식학회지
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• 제9권2호
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• pp.133-139
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• 1985

An immunoassay may be defined as an analytical procedure involving the competitive reaction between a limiting concentration of specific antibody and two populations of antigen, one of which is labelled or immobillized. The advent of immunoassay has revolutionised our knowledge of reproductive physiology and the practice of veterinary and clinical medicine. Radioimmunoassay (RIA) was the first of these methods to be developed, which meausred the analyte with good sensitivity, accuracy and precision (1,2). The essential components of RIA are:-(i) a limited concentration of antibodies, (ii) a reference preparation, and (iii) an antigen labelled with a radioisotope (usually tritium or iodine-125). Most procedures invelove isolating the antibody-bound fraction and measuring the amount of labelled antigen. Good facilities are available for scintilltion counting, data reduction nd statistical analysis. RIA is undergoing refinement through:-(i) the introduction of new techniques to separate the antibody-bound and free fractions which minimize the misclassification of labelled antigen into these compartments, and the amount of non-specfic binding. (3), (ii) the development of non-extration for the measurement of haptens (4), (iii) the determination of a, pp.rent free (i.e. non-protein bound) analytes (5), and (iv) the use of monoclonal antibodies(6). In 1968, Miles and Hales introduced in important new type of immunoassay which they termed immunora-diometric assay (IRMA) based on t도 use of isotopically labelled specific antibodies(7) in a move from limited to excess reagent systems. The concept of two-site IRMAs (with a capture antibody on a solid-phase, and a second labelled antibody to a different antigenic determinant of the analyte) has enabled the development of more sensitive and less-time consuming methods for the measurement of protein hormones ovar wide concentration of analyte (8). The increasing use of isotopic methos for diverse a, pp.ications has exposed several problems. For example, the radioactive half-life and radiolysis of the labelled reagent limits assay sensitivity and imposes a time limit on the usefulness of a kit. In addition, the potential health hazards associated with the use and disposal of radioactive cmpounds and the solvents and photofluors necessary for liquid scientillation counting are incompatable with the development of extra-laboratory tests. To date, the most practical alternative labels to radioisotopes, for the measurement of analytes in a concentration > 1 ng/ml, are erythrocytes, polystyrene particiles, gold sols, dyes and enzymes or cofactors with a visual or colorimetric end-point(9). Increased sensitivity to<1 pg/ml may be obtained with fluorescent and chemiluminescent labels, or enzymes with a fluorometric, chemiluminometric or bioluminometric end-point. The sensitivity of any immunoassay or immunometric assay depends on the affinity of the antibody-antigen reaction, the specific activity of the label, the precision with which the reagents are manipulated and the nonspecific background signal (10). The sensitivity of a limited reagent system for the measurement of haptens or proteins is mainly dependent upon the affinity of the antibodies and the smalleest amount of reagent that may be manipulated. Consequently, it is difficult in practice to improve on the sensitivity obtained with iodine-125 as the label. Conversely, with excess reagent systems for the measurement of proteins it is theoretically possible to increase assay sensitivity at least 1000 fold with alternative luminescent labels. To date, a 10-fold improvement has been achieved, and attempts are being made to reduce the influence of other variables on the specific signal from the immunoreaction.

• 한국지구과학회지
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• 제27권2호
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• pp.221-235
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• 2006

조간대성 해빈 퇴적물의 이동양상을 파악하기 위하여 한반도 남해안의 득량만에서 해빈 주변 표층퇴적물과 해빈 단면, 해빈 퇴적물, 해빈 퇴적률, 그리고 해빈 수리에너지 특성에 대한 조사를 실시하였다. 수문 해빈은 급경사의 해빈면과 완경사의 저조단구로 이루어져 일반적으로 조차가 파고에 비해 큰 해빈 단면의 특징을 보인다. 그러나 만입된 안의 중앙 해빈면은 여름에 평탄해지고 그 밖의 계절에는 파봉과 유흔이 발달하는 등 뚜렷한 계절변화를 보인다. 이러한 해빈 단면의 계절변화는 연구지역의 해빈 퇴적작용이 주로 조석에 의해 지배되지만 파랑의 영향도 강하게 받고있음을 의미하는 것으로, 해빈 퇴적률의 계절변화에서도 잘 나타난다. 즉, 겨울에는 강한 파랑의 영향에 의해 침식이 우세한 반면, 그 밖의 계절에는 상대적인 저에너지 조건 하에서 퇴적이 우세한 특징을 보인다. 그러나 해빈의 중앙은 여름에도 뚜렷하게 침식되는 경향을 보이는데, 이는 여름에 간헐적으로 발생한 남풍 계열의 강한 바람에 의한 파랑이 해빈에 강하게 미치기 때문이다. 한편, 해빈 퇴적물은 만입된 해빈의 중앙에서는 -89.2 mm/yr로 침식된 반면, 양쪽 측면에서는 각각 60.5 mm/yr와 38.2 mm/yr로 퇴적되는 경향을 보인다. 이와 같은 현상은 해빈의 중앙에 분포하는 퇴적물이 파랑이 강화되면 침식되어 양쪽 측면으로 이동하기 때문으로 생각된다. 따라서 해수욕장으로 주로 이용되는 수문 해빈의 중앙은 방파제 건설에 따른 조립질퇴적물의 공급이 제한되어 있음을 고려할 때 지속적으로 침식될 것으로 판단된다.

• Journal of Advanced Marine Engineering and Technology
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• 제38권7호
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• pp.890-899
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• 2014

오스테나이트계 스테인리스강은 우수한 내식성으로 인해 다양한 산업에 널리 적용되는 재료이다. 그러나 열처리나 용접 실시 후 입계에 크롬 탄화물 생성으로 크롬 결핍대가 형성되어, 입계의 내식성이 상대적으로 취약해지는 문제점이 있다. 이를 해결하기 위해 Ti 또는 Nb과 같은 탄소 안정화 원소를 첨가하여 크롬 탄화물 생성을 억제한다. 이러한 안정화된 스테인리스강에 대한 해수환경하에서의 내식성에 관한 연구는 적은 실정이다. 본 연구에서는 안정화 원소(Ti 및 Nb)를 함량 변수로 첨가한 스테인리스강에 대해 해수환경하에서 전기화학적 특성을 평가하고자 하였다. 이를 위해 합금원소 첨가에 따른 미세조직의 변화를 관찰하였으며, 자연전위 측정과 동전위분극 실험을 통해 전기화학적 특성을 파악하였다. 미세조직 관찰 결과, 모든 시편에서 오스테나이트 기지상 이외에 합금원소 첨가에 따른 개재물이 관찰되었다. 이러한 개재물은 기지 조직과 상이한 전기화학적 특성을 가지는 것으로 판단되며, 안정화 원소의 종류 및 함량에 따라 뚜렷한 전기화학적 특성 차이를 나타냈다. 본 연구 결과 오스테나이트계 스테인리스강에 내식성 향상을 위해 첨가되는 Ti 또는 Nb은 첨가량에 따라 서로 다른 전기화학적 특성을 나타내므로, 이를 고려한 합금 설계가 중요할 것으로 사료된다.

• 한국대기환경학회지
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• 제31권3호
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• pp.239-254
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• 2015

Little information on HUmic-Like Substances (HULIS) in ambient particulate matter has been reported yet in Korea. HULIS makes up a significant fraction of the water-soluble organic mass in the atmospheric aerosols and influence their water uptake properties. In this study 24-hr $PM_{2.5}$ samples were collected between December 2013 and October 2014 at an urban site in Gwangju and analyzed for organic carbon (OC), elemental carbon (EC), water-soluble OC (WSOC), HULIS, and ionic species, to investigate possible sources and formation processes of HULIS. HULIS was separated using solid phase extraction method and quantified by total organic carbon analyzer. During the study period, HULIS concentration ranged from 0.19 to $5.65{\mu}gC/m^3$ with an average of $1.83{\pm}1.22{\mu}gC/m^3$, accounting for on average 45% of the WSOC (12~ 73%), with higher in cold season than in warm season. Strong correlation of WSOC with HULIS ($R^2=0.91$) indicates their similar chemical characteristics. On the basis of the relationships between HULIS and a variety of chemical species (EC, $K^+$, $NO_3{^-}$, $SO_4{^{2-}}$, and oxalate), it was postulated that HULIS observed during summer and winter were likely attributed to secondary formation and primary emissions from biomass burning (BB) and traffics. Stronger correlation of HULIS with $K^+$, which is a BB tracer, in winter ($R^2=0.81$) than in summer ($R^2=0.66$), suggests more significant contribution of BB emissions in winter to the observed HULIS. It is interesting to note that BB emissions may also have an influence on the HULIS in summer, but further study using levoglucosan that is a unique organic marker of BB emissions is required during summer. Higher correlation between HULIS and oxalate, which is mainly formed through cloud processing and/or photochemical oxidation processes, was found in the summer ($R^2=0.76$) than in the winter ($R^2=0.63$), reflecting a high fraction of secondary organic aerosol in the summer.

• 대한약리학회지
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• 제23권2호
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• pp.57-75
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• 1987

Two modalities of gonadotropin secretion, pulsatile gonadotropin and preovulatory gonadotropin surge, have been identified in the mammals. Pulsatile gonadotropin secretion is modulated by the pulsatile pattern of GnRH release and complex ovarian steroid feedback actions. The neural mechansim that regulates the pulsatile release of GnRH in the hypothalamus is called "GnRH pulse generator". Ovarian steroids, estradiol and progesterone, appear to exert thier feedback effects both directly on the pituitary to modulate gonadotropin release and on a hypothalamic site to modulate GnRH release; estradiol primarily affects the amplitude while progesterone decreases the frequency of the pulsatile GnRH. Steroid hormones are known to affect catecholamine transmission in brain. MBH-POA is richly innervated by NE systems and close apposition of NE terminals and GnRH cell bodies occurs in the MBH as well as in the POA. NE normally facilitates pulsatile LH release by acting through ${\alpha}-receptor$ mechanism. However, precise nature of facilitative role of NE transmission in maintaining pulsatile LH has not been clearly understood. Close apposition of DA and GnRH terminals in ME might permit DA to influence GnRH release. Action of DA transmission probably is mediated by axo-axonic contacts between GnRH and DA fibers in the ME. Dopamine transmission does not normally regulate pulsatile LH release, but under certain conditions, increased DA transmission inhibit LH pulse. Endogenous opioid acts to suppress the secretion of GnRH into hypophysial portal circulation, thereby inhibiting gonadotropin secretion. However, an interaction between endogenenous opioid peptides and gonadotropin release is a complex one which involves ovarian hormones as well. LH secretion appears to be most suppressed by endogenenous opioids during the luteal phase, at a time of elevated progesterone secretion. The arcuate nucleus contains not only cell bodies for GnRH and ${\beta}-endorphin$ but also a dense aborization of fibers suggesting that GnRH release is changed by the interactions between GnRH and ${\beta}-endorphin$ cell bodies within the arcuate nucleus. The frequency and amplitude of pulsatile LH release seem to be increased during the preovulatory gonadotropin surge. Estradiol exerts positive feedback action on the hypothalamo-pituitary axis to trigger preovulatory LH surge. GnRH is also crucial hormonal stimulus for preovulatory LH surge. It is unlikely, however, that increased secretion of GnRH during the preovulatory gonadotropin surge represents an obligatory neural signal for generation of the LH discharge in primates including human. Modulation of preovulatory LH surge by catecholamines has been studied almost exclusively in rats. NE and E may be involved in distinct way to accumulate GnRH in the MBH and its release into the hypophysial portal system during the critical period for LH surge on proestrus in rats. However, the mechanisms whereby augmented adrenergic transmission may facilitate the formation and accumulation of GnRH in the ME-ARC nerve terminals before the LH surge have not been clearly understood.

• 한국농공학회지
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• 제16권2호
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• pp.3438-3453
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• 1974

Unmeasured value of water for human lives is widely approved, but the water as one of natural resources cannot be evaluated with ease since it changes itself ceaselessly by flowing-out or transforming the phase. Major objectives of the study concerned consequently with investigating its potentiality and evaluating its time seriesly availabity in a volumatic unit. And the study was performed to give the accurate original data to the planners concerned. Some developed rational methods of predicting runoff related to hydrological factors as precipitation, were to be discusseed for their theorical background and to be introduced whether they needed some corrections or not, comparing their estimation with actual runoff from synthetic unit-hydrograph methods. To do so, the study was performed to select Kongju Station, located at the watershed of the Keum River, and to collect such hydrological data from 1962 to 1972 as runoff, water level, precipitation, and so on. On the other hand, the hydrological characteristics of runoff were concluded more reasonably in numerical values, with calculating the the ratio of daily runoff to annual discharge of the flow in percentage, as. the distribution ratio of runoff. The results of the study can be summarized as follows; (1) There needed some consideration to apply the Kajiyama's Formula for predicting monthly runoff of rivers in Korea.(2) The rational methods of predicting runoff might be recommended to become less theorical and reliable than the unique analyzation of data concerned in each given water basin. The results from the Keum River prepared above would be available to any programms concerned. (3) The most accurate estimation for runoff could be suggested to synthetic unithydrograph methods calculated from the relation between each storm and runoff. However it was not contained in the study. (4) The relations between rainfall and runoff at KongJu Station were as following table. The table showed some intersting implications about the characteristics of runoff at site, which indicated that the runoff during three months from July to September approached total of 60% of quantity while precipitation concentrated on the other three from June to August. And there were some months which had more amount of runoff than expected values calculated from the precipitation, such as Febrary, March, August, September, Octover, and December, shown in the table. Such implications should be suggested to meet any correction factors in the future formulation concerned with the subjects, if any rational methods would be required.

• 한국작물학회지
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• 제33권4호
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• pp.353-359
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• 1988

두께가 서로 다른 $C_3$ 식물의 잎은 단위엽면적당 광합성 능력에 있어서도 차이가 나는 바 잎의 내부구조와 기체교환 사이의 관계를 바탕으로 그 원인을 구명하였다. 광합성의 2대 제한요인으로 기체확산과 생화학적 과정의 상대적인 중요도를 결정하기 위해 중엽세포의 표면은 기체확산 저항의, 그리고 세포의 체적은 탄소고정 능력의 지표로 가정하였다. 즉 세포의 표면적이 증가하면 이산화탄소의 액상확산 저항이 감소하며 체적이 증대되면 carboxylation, oxygenation, 그리고 dark respiration 반응속도가 증가한다고 간주하였다. 이러한 개념을 함축하는 광합성 모형을 작성하고 이 가설의 검증을 위해 대두 품종 Amsoy잎을 이용한 실험을 수행하였다. 생장조절실내에서 200, 400, 600$\mu$mol photons m$^{-2}$ s$^{-1}$ PAR을 공급하여 서로 다른 두께의 잎을 준비하였으며 제3 및 4본엽에 대해 1,000 $\mu$mol photons m$^{-2}$ s$^{-1}$ PAR 및 28 기온 환경하에서 이산화탄소 흡수속도를 측정한 결과 세포의 체적과 표면적의 영향을 동시에 고려한 광합성 모형이 세포 표면적만을 고려한 경우 보다 실측치에 가까운 예측치를 산출하였다. 이로 미루어 세포의 표면적과 체적은 잎의 두께 및 그에 따른 광합성 능력의 예측에 적절한 변수로 간주된다.

• Journal of Radiation Protection and Research
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• 제45권3호
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• pp.101-107
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• 2020

Background: An important lesson learned from the Fukushima accident is that the transition to the mid- and long-term phases from the emergency-response phase requires less than a year, which is not very long. It is necessary to know how much radioactive material has been deposited in an urban area to establish mid- and long-term countermeasures after a radioactive accident. Therefore, an urban deposition model that can indicate the site-specific characteristics must be developed. Materials and Methods: In this study, the generalized urban deposition velocity and the subsequent variation in radionuclide contamination were estimated based on the characteristics of the Korean urban environment. Furthermore, the application of the obtained generalized deposition velocity in a hypothetical scenario was investigated. Results and Discussion: The generalized deposition velocities of ¹³⁷Cs, ¹⁰⁶Ru, and ¹³¹I for each residence type were obtained using three-dimensional (3D) modeling. For all residence types, the deposition velocities of ¹³¹I are greater than those of ¹⁰⁶Ru and ¹³⁷Cs. In addition, we calculated the generalized deposition velocities for each residential types. Iodine was the most deposited nuclide during initial deposition. However, the concentration of iodine in urban environment drastically decreases owing to its relatively shorter half-life than ¹⁰⁶Ru and ¹³⁷Cs. Furthermore, the amount of radioactive material deposited in nonresidential areas, especially in parks and schools, is more than that deposited in residential areas. Conclusion: In this study, the generalized urban deposition velocities and the subsequent deposition changes were estimated for the Korean urban environment. The 3D modeling was performed for each type of urban residential area, and the average deposition velocity was obtained and applied to a hypothetical accident. Based on the estimated deposition velocities, the decision-making systems can be improved for responding to radioactive contamination in urban areas. Furthermore, this study can be useful to predict the radiological dose in case of large-scale urban contamination and can support decision-making for long-term measurement after nuclear accident.

• 한국건설관리학회논문집
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• 제13권1호
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• pp.118-128
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• 2012

1980년대 이후 국내 아파트에서 적용되어온 벽식구조는 리모델링 시 많은 문제점을 유발시켜 정부에서는 법적 인센티브를 제공하며 무량판 및 라멘구조를 장려하고 있다. 이에 따라 기존의 골조의 문제점을 개선한 친환경 라멘조인 그린 프레임이 개발되어 구조적 안전성 뿐 아니라 시공성, 친환경성에 대한 검증이 이루어졌다. 그린프레임의 경우 설계단계에서 작성된 프리캐스트 콘크리트(Precast Concrete; PC) 부재 정보를 이용하여 물량 산출 및 철근 가공도(bar bending schedule) 등을 자동으로 작성하면 인력저감 뿐 아니라 철근 손율(loss)을 줄이는 철근조합을 용이하게 수행할 수 있다. 따라서 본 연구는 합성 PC부재에 의해 설계된 그린 프레임의 철근물량 산출 자동화 알고리즘을 개발하는 것을 목적으로 한다. 철근물량 산출자동화 알고리즘은 구조 설계정보, 시방정보, 합성PC의 철골정보 등을 이용하여 작성한 후 사례현장 적용을 통하여 개발된 알고리즘의 효용성을 증명한다. 개발된 알고리즘에 의해 저장된 정보는 철근가공조립도, 철근 재단 리스트(bar cutting list)작성 자동화 뿐 아니라 철근 손율을 최소화 할 수 있는 최적조합과 주문물량 산출 자동화에도 활용될 것이다. 또한 공사관리인력 저감 뿐 아니라 철근 손율 최소화 관리에 따른 공사 원가절감의 효과를 기대할 수 있다.

• 한국초등과학교육학회지:초등과학교육
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• 제40권1호
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• pp.113-125
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• 2021

In this study, a problem-centered learning based STEAM field experience learning program was developed and the effects of applying it were investigated. The program was composed of 8 sessions by using problem-centered learning education method and integrating STEAM elements between disciplines. The contents of program are as follow. In the step of sharing problems and making a problem-solving plan, they understood the various examples and meanings of endangered species, explored the project activities, and made an inquiry plan. In the search and re-exploration phase, a smart device was used to investigate the appearance, habitat environment and cause of extinction for Clithon retropictus, and a site inquiry plan was established for each group. Then, they moved to the field to explore brackish-headed gallops and discuss ways to protect endangered species. In the step of creating a solution, a web-based report was produced as the final product using smart devices based on the results of the inquiry. In the presentation and evaluation stage, the produced web-based report was used to present each group, conduct mutual evaluation, and organize project activities. The developed program was applied to 6th grade 29 students enrolled in the J University Gifted Education Center. In order to find out the effectiveness of the program, tests of science process skill, creative problem-solving ability, and scientific attitude were conducted before and after of program learning, and the results were statistically analyzed by t-test. In addition, a STEAM program satisfaction test was conducted after project in order to find out the satisfaction of the class. As a result of application of the program, the results were significantly improved in openness, criticism, and creativity among the sub-factors of creative problem-solving ability and scientific attitude. Satisfaction with the STEAM program was also high, but no significant result was found in science process skill. Therefore, the program of this study could be influenced on improvement of creative problem-solving ability and scientific attitude of gifted students in elementary science.