• Journal of the Korean Society for Marine Environment & Energy
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• v.19 no.2
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• pp.87-98
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• 2016

During the period between July 3 and 27 of 2009, water samples were collected from the Russian coast at a depth of 30m from 26 stations (including Ulleung and Japan basins) onboard the Russian survey vessel R/V Lavrentyev following 4 lines (D, R, E, and A). The samples were analyzed for nutrients and chlorophyll a contents. All parameters exhibited higher values in warm waters than in cold waters ($NH_4:1.8-fold$, $PO_4:1.8-fold$, $SiO_2:1.2-fold$, and chlorophyll-${\alpha}$:1.9-fold), except nitrates, which was 1.4-fold higher in cold waters than in warm waters. The horizontal distribution of ammonia, phosphate, and chlorophyll-${\alpha}$ was very similar to each other and showed the highest values in the waters near Russia, where a upwelling influence of cold current and bottom water prevails, while relatively low distribution was observed at the Ulleung Basin. On the other hand, nitrates showed the highest concentration at the Ulleung Basin, which is under the direct influence of the Tsushima warm water, and showed a gradual decrease northward. The N/P ratio showed the highest value in the Tsushima middle water, rather than in the North Korean Cold Water, the Tsushima Warm Water was the primary source of nitrate flow into the East Sea. However, the average concentration of phosphate in the warm waters was < $0.2{\mu}M$, thereby limiting phytoplankton growth, while a high concentration of phosphate in cold waters showed a direct correlation with chlorophyll-${\alpha}$. The results of principal component analysis for the identification of primary factors that influence the marine environment showed that principal component I was water temperature and principal component II was influenced chlorophyll-${\alpha}$ and nutrients. Therefore, Study area has greatest influenced by water temperature, and clearly distinct cold and warm water regions were observed in the East Sea.

• Economic and Environmental Geology
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• v.50 no.6
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• pp.477-486
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• 2017

In this study, we analyze precise seabed geomorphology and conditions for comparing the nearshore areas of the Dongdo(East Island) and the Seodo(West Island) using detailed bathymetry data and seafloor backscattering images, in Dokdo, the East Sea. We have been obtained the detailed bathymetry data and the seafloor backscattering data. The survey range is about $250m{\times}250m$ including land of islets to the nearshore areas of the southern part of the Dongdo and the Seodo. As a result of bathymetry survey, the southern area of the Dongdo(~50 m) is deeper than the Seodo(~30 m) in the water depth. The survey areas are consist of extended bedrocks from land of the Dongdo and the Seodo. The underwater rock region of the Seodo is larger than the Dongdo. In spite of similar extended rocks features from islets, there are some distinctive seabed characteristics between the southern nearshore areas of the Dongdo and the Seodo. The Talus-shaped seafloor environment formed by gravel and underwater rocks originating from the land of the Dongdo is up to about 15 m depth. And the boundary line of between extended bedrocks and seabottom is unclear in the southern nearshore of the Dongdo. On the other hand, the southern coast of the Seodo is characterized by relatively large scale underwater rocks and evenly distributed sediments, which clearly distinguish the boundary of between extended bedrocks and seafloor. This is because the tuff layers exposed to the coastal cliffs of the Dongdo are weak against weathering and erosion. It is considered that there are more influences of the clastic sediments carried from the land of the Dongdo compared with the Seodo. Particularly, the land of the Dongdo has been undergoing construction activities. And also a highly unstable ground such as faults, joints and cracks appears in the Dongdo. In previous study, there are dissimilar features of the massive tuff breccia formations of the Dongdo and the Seodo. These conditions are thought to have influenced the different seabed characteristics in the southern nearshore areas of the Dongdo and the Seodo.

• Journal of Korean Home Economics Education Association
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• v.19 no.3
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• pp.37-60
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• 2007

This study has been historically reviewed the clothing and textiles education through references. The clothing and textiles education had been informally offered as a part of household skill, skill of daily life and home skill before the law of school education was established. Therefore, we have reviewed the changes in the clothing and textiles education by separating the prior period between the end of the old Korea and the liberation in 1945, from the later period from the liberation up to now. And also contents of clothing and textiles education between before and after the revision of the 7th curriculum were compared and analyzed. The clothing and textiles education had put emphasis on sewing, handicraft and household during the prior period from the end of the old Korea to the liberation, but after liberation, it became involved in Home Economics Education. The number of classes and the contents of clothing and textiles education had been increased and deepened from the 1st term to the 5th term of curriculum. In particular, the 4th and 5th term of curriculum became a turning point where home life section was intensified. Even though the number of classes was reduced during the 6th term of curriculum, with increase in target school grades for this education, and Home Economics in the middle school remained an independent subject matter. However, during the 7th term of curriculum, Technology-Home Economics Education in the middle school courses led to losing independent subject name and shrinking its contents. Through the revision in the 7th curriculum, the clothing and textiles education achieved great improvement and progress by gaining balance in distribution of contents among school grades and balance between theory and practice. However it still remains as an issue to be more studied and resolved how well the contents are matched with the interests and attention of students. We need to consider and reflect request of students under learner-centered curriculum. Considering enormous changes in the environment surrounding the clothing and textiles education, it is crystal clear that the gap between existing contents of education and teaching methods and our real life will be widened. Because students' perceptions of value have been diversified, it is our task to develop better contents and learning system in order to help students have interests, attention and desire in clothing and textiles life in line with social needs for desirable clothing and textiles education.

• Journal of Oral Medicine and Pain
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• v.34 no.3
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• pp.261-276
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• 2009

Lactacystin, a microbial natural product synthesized by Streptomyces, has been commonly used as a selective proteasome inhibitor in many studies. Proteasome inhibitors is known to be preventing the proliferation of cancer cells in vivo as well as in vitro. Furthermore, proteasome inhibitors, as single or combined with other anticancer agents, are suggested as a new class of potential anticancer agents. This study was undertaken to examine in vitro effects of cytotoxicity and growth inhibition, and the molecular mechanism underlying induction of apoptosis in SCC25 human tongue sqaumous cell carcinoma cell line treated with lactacystin. The viability of SCC25 cells, human normal keratinocytes (HaCaT cells) and human gingiva fibroblasts (HGF-1 cells), and the growth inhibition of SCC25 cells were assessed by MTT assay and clonogenic assay respectively. The hoechst staining, hemacolor staining and TUNEL staining were conducted to observe SCC25 cells undergoing apoptosis. SCC25 cells were treated with lactacystin, and Western blotting, immunocytochemistry, confocal microscopy, FAScan flow cytometry, MMP activity, and proteasome activity were performed. Lactacystin treatment of SCC25 cells resulted in a time- and does-dependent decrease of cell viability and a does-dependent inhibition of cell growth, and induced apoptotic cell death. Interestingly, lactacytin remarkably revealed cytotoxicity in SCC25 cells but not normal cells. And tested SCC25 cells showed several lines of apoptotic manifestation such as nuclear condensation, DNA fragmentation, the reduction of MMP and proteasome activity, the decrease of DNA contents, the release of cytochrome c into cytosol, the translocation of AIF and DFF40 (CAD) onto nuclei, the up-regulation of Bax, and the activation of caspase-7, caspase-3, PARP, lamin A/C and DFF45 (ICAD). Flow cytometric analysis revealed that lactacystin resulted in G1 arrest in cell cycle progression which was associated with up-regulation in the protein expression of CDK inhibitors, $p21^{WAF1/CIP1}$ and $p27^{KIP1}$. We presented data indicating that lactacystin induces G1 cell cycle arrest and apoptois via proteasome, mitochondria and caspase pathway in SCC25 cells. Therefore our data provide the possibility that lactacystin could be as a novel therapeutic strategy for human tongue squamous cell carcinoma.

• Journal of the Korean Society of Marine Environment & Safety
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• v.16 no.2
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• pp.175-183
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• 2010

To understand the variation of macro benthos community according to the installation of structure and topographic placement in the shellfish farm on tidal flat, the practical example of the tidal shellfish growing area at Namsung-ri Goheung was observed. The results of the research for the field observation were summarized as follows. (1) The ground gradient of the shellfish farm was very flat below about $1^{\circ}$. The shellfish farm ground took the shape of $\sqcup$ from the shoreline to the place of 150 m seawards, and the shape of $\sqcap$ from there to the low tide line. During ebb tide, the $\sqcup$ shape ground stored the sea water, and the $\sqcap$ shape ground was supposed to act as the effect factor to leak slowly or to prevent the outflow. (2) The oyster shell bag or the type of riprap wall as the boundary in the shellfish farm was classified into five types. The air exposure time and flooding time were 181 and 434 minutes, respectively. (3) In the numerical experiment, the deep-sea water wave coming in the study area had 0.5 m of maximum wave height to show the very stable conditions and the wave direction pattern of S-direction was dominant at Naro great ridge, and SE, SSW and S-direction were distributed strongly around the shellfish farm. (4) By the grain size analysis, the sediment around tidal flat consisted of gravel 0.00~5.81(average 1.70)%, sand 14.15~18.39(average 13.23)%, silt 27.59~47.15(average 30.84)% and clay 35.79~55.73(average 36.19)%, and the sediment type was divided into (g)M(lightly gravelly mud), sM(sandy mud) and gM(gravelly mud) by Folk's diagram. (5) The macro benthos community survey conducted in this site in January, 2010 showed that 1 species of Mollusca, 8 species of Polychaeta and 2 species of Crustacea appeared, and 11 species occupying over 1% of total abundance were dominant.

• The Journal of the Petrological Society of Korea
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• v.22 no.1
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• pp.19-34
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• 2013

The Ipcheon Subbasin is an isolated Miocene basin in SE Korea, which has the geometry of an asymmetric graben elongated in the NE-SW direction. It is in contact with basement rocks by faults and separated from adjacent Waup and Eoil basins by the basement. The strata of the basin fills have an overall homoclinal structure, dipping toward NW or WNW. The basin fills consist of Early Miocene sediments rich in dacitic volcanic and volcaniclastic deposits and Middle Miocene non-volcanic and nonmarine conglomerates intercalated with sand layers, which are distributed in the northeastern and southwestern parts of the basin, respectively. Kinematic analysis of syndepositional conjugate faults in the basin fills indicates WNW-ESE extension of the basin. These features are very similar to those of the adjacent Waup and Eoil basins, indicating that the basin extension was governed by the NE-trending northwestern border faults and that the basin experienced a propagating rifting from NE to SW. Basaltic materials, which occur abundantly in the Eoil Basin, are totally absent in the Ipcheon Subbasin. The observations of the dacitic tuff and tuffaceous mudstone in the subbasin, on slabs and under microscope, suggest that they have lithologies very similar to those of the Yondongri Tuff in the Waup Basin. The Middle Miocene non-volcanic sediments of the Waup and Eoil basins and the Ipcheon Subbasin are distributed consistently in the southwestern part of each basin. It is thus concluded that the extension of the Ipcheon Subbasin began at about 22 Ma together with the Waup Basin and was lulled during the main extension period of the Eoil Basin between 20-18 Ma. At about 17 Ma, the subbasin was re-extended due to the activation of the Yeonil Tectonic Line associated with the propagating rifting toward SW. This event is interpreted to have provided new sedimentation space for the Middle Miocene sediments in the southwestern parts of the Waup and Eoil basins and the Ipcheon Subbasin as well.

• Korean Journal of Medicinal Crop Science
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• v.10 no.1
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• pp.46-50
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• 2002

In DNA level, genetic study of Angelica species was firstly conducted to discriminate the bolting-resistant or low bolting variety, so called as Manchu, from other Korea collected lines and also this technuque was applied to identify the origin of commercial dried materials obtained from current oriental medicinal market. By RAPD analysis with 72 primers including sixty of 10-mers and twelve of 20-mers, respectively, three primers, which were related to the bolting resistant traits of Angelica gigas, were identified. Comparing the RAPD bands, URP04 primer showed the 1.7 kb specific band, which seemed to be related to delaying bolting traits, since it was observed only in Jinbu elite lines but not in others. On the other hand, since 1.2 kb band amplified by OPD11 was observed in other collected lines but not in Manchu var. and Jinbu line, this primer also could be considered as a selection marker for identifying bolting resistant or delaying bolting traits. In the same manner, since OPP09 did not show 1 kb major band but produced 0.8 kb and 1.2 kb bands in Manchu var., these three bands amplified by the primer could be considered one of the important key specifying Manchu var. related with the trait of Angelica gigas. OPC02 primer showed the same band patterns in all Korean collected lines, but not in other foreign introduced lines, such as A. sinensis from China, and A. acutiloba from Japan. Since these four RAPD primers, OPD11, OPP09, URP04, and OPC02 showed the specific polymorphisms in Angelica species, thus, these were useful to discriminate the three Angelica species, A. gigas, A. sinensis, and A. acutiloba.

• Tuberculosis and Respiratory Diseases
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• v.48 no.6
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• pp.898-908
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• 2000

Background : Eotaxin a CC chemokine specific for eosinophils, is implicated in the pathogenesis of asthma by recruiting eosinophils into the airways. Theophylline has been used for the treatment of asthma and recently was proposed to have an anti-inflammatory action. The aim of this study is to examine whether theophylline may inhibit the eosinophilic airway inflammation by reducing the expression of eotaxin. Methods : The expression of eotaxin mRNA was assessed by Northern analysis in A549 cells 4 h after stimulation with TNF-$\alpha$ or IL-1$\beta$. And then, theophylline was added to A549 cells stimulated with 0.1 ng/mL IL-1$\beta$. Results : Eotaxin mRNA expression rates induced by 0.1, 1, 10 ng/mL TNF-$\alpha$ as compared with $\beta$-actin, were 7%, 22%. 28%, respectively. Eotaxin mRNA expression rates induced by 0.01, 0.1, 1, 10 ng/mL IL-1$\beta$, as compared with $\beta$-actin, were 10%, 42%, 63%, 72%, respectively. Eotaxin mRNA expression rates after the addition of 0, 0.001, 0.01, 0.1 ${\mu}M$ dexamethasone induced by 10 ng/mL TNF-$\alpha$ as compared with $\beta$-actin, were 27%, 18%, 8%, respectively. Eotaxin mRNA expression rate after the addition of 0, 0.001, 0.01, 0.1 ${\mu}M$ dexamethasone induced by 0.1 ng/mL IL-1$\beta$ as compared with $\beta$-actin, were 43%, 47%, 12%, 8%, respectively. Eotaxin mRNA expression rates after the addition of 0, 0.001, 0.01, 0.1, 1, 10 mM theophylline induced by 0.1 ng/mL IL-1$\beta$, as compared with $\beta$-actin, were 48%, 40%, 33%, 22%, 16%, 14%, respectively. Conclusion : These results suggest that theophylline may reduce eosinophil infiltration of the airway at least in part by reducing the expression of eotaxin under the conditions of these experiments.

• Tuberculosis and Respiratory Diseases
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• v.40 no.6
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• pp.653-658
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• 1993

Background: Recent advancement of molecular genetics has revealed that malignant transformation of a cell may be a complex multistep process and this process is grouped, in general, into two distinct categories, activation of protooncogenes and inactivation of tumor suppressor genes. This study was focused on the mutation of p53 tumor suppressor gene, because p53 gene mutation is now generally accepted to be one of the most frequent genetic changes in a variety of human cancers. Although lung cancer is one of the common cancers in Korea, the genetic change in the carcinogenesis process is not yet known clearly. To investigate the role of p53 gene mutation in lung cancer, we examined the mutations of exon 4-8 of the p53 gene in humna lung cancer cell lines, because most of the mutations of p53 gene have been reported to develop in exon 4-8. Method: Genomic DNA was obtained by the digestion of proteinase K and the extraction by phenol-chloroform-ethanol method from two human pulmonary adenocarcinoma cell lines, PC-9 and PC-14, and one human small cell lung cancer cell line, H69. To detect the mutations of exon 4-8 of the p53 gene, polymerase chain reaction single-strand conformation polymorphism(PCR-SSCP) analysis was performed with the DNA extracted from the cells. Results: The mutation of p53 gene was present in all three cell lines tested. In PC-9, PC-14 and H69, the altered mobility was detected in exon 7, 7 and 5, respectively. Conclusion: These results suggest that p53 gene mutation plays an important role in certain steps of the carcinogenesis of human non-small cell and small cell lung cancer.

• Journal of The Korean Association For Science Education
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• v.30 no.6
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• pp.693-718
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• 2010

This study investigated secondary school science teachers' experiences to explore the influencing factors in science teachers efficiency (STE). The participants, thirty three secondary school science teachers who have more than four years of teaching experience, were interviewed about describing each teacher's experience throughout one's years of teaching. The grounded theory introduced by Strauss and Corbin (1998) was used to analyze the data in this study. The results of paradigm analysis revealed that STE is influenced by 125 concepts, 38 sub-categories, and 16 categories. In a paradigm model, the central phenomenon was 'constructing STE', and the causal condition was 'want to be a teacher' as career choice motivation. The contextual conditions that have an affect on the central phenomenon were 'self awareness of the teacher' and 'social awareness of the teacher.' The mediate conditions, which facilitated or restrained the action/interaction strategies, were 'societal tendency', 'school climate', and 'personal context.' The action/interaction strategies to control the phenomenon were 'following the line,' 'identifying effective teaching strategies,' 'taking teacher education programs,' and 'contributing to school improvement.' The consequences were 'teacher's self awareness', 'challenge,' and 'stagnating in teaching.' The overall conclusion drawn from this research is that, the definition of STE is beliefs in science teachers' capabilities to set up objects in some school teaching context and, organize and execute the course of action required to attain these. Additionally, STE has three dimensions of teacher's behaviors: science instructional efficiency, efficiency in engaging students, and efficiency in managing school conditions. This study offers insight into the nature of STE and theoretical framework. These findings may give science teachers and teacher educators the practical knowledge necessary to build effective training programs and interventions that would help increase STE and facilitate effective teaching.