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Selection of RAPD marker to discriminate the bolting-resistant varieties and commercial dried medicinal materials of Angelica species  

Bang, Kyong-Hwan (Industrial Crop Div., Nat,l Crop Exp. Station, RDA)
Yu, Hong-Seob (Industrial Crop Div., Nat,l Crop Exp. Station, RDA)
Koo, Dal-Hoe (Dept. of Biology, Chungnam Nat,l Unv.)
Cho, Joon-Hyeong (Industrial Crop Div., Nat,l Crop Exp. Station, RDA)
Park, Hee-Woon (Industrial Crop Div., Nat,l Crop Exp. Station, RDA)
Seong, Nak-Sul (Industrial Crop Div., Nat,l Crop Exp. Station, RDA)
Park, Sang-Il (Dept. of Plant Resources, Chungbuk Nat,l Unv.)
Kim, Hong-Sig (Dept. of Plant Resources, Chungbuk Nat,l Unv.)
Publication Information
Korean Journal of Medicinal Crop Science / v.10, no.1, 2002 , pp. 46-50 More about this Journal
Abstract
In DNA level, genetic study of Angelica species was firstly conducted to discriminate the bolting-resistant or low bolting variety, so called as Manchu, from other Korea collected lines and also this technuque was applied to identify the origin of commercial dried materials obtained from current oriental medicinal market. By RAPD analysis with 72 primers including sixty of 10-mers and twelve of 20-mers, respectively, three primers, which were related to the bolting resistant traits of Angelica gigas, were identified. Comparing the RAPD bands, URP04 primer showed the 1.7 kb specific band, which seemed to be related to delaying bolting traits, since it was observed only in Jinbu elite lines but not in others. On the other hand, since 1.2 kb band amplified by OPD11 was observed in other collected lines but not in Manchu var. and Jinbu line, this primer also could be considered as a selection marker for identifying bolting resistant or delaying bolting traits. In the same manner, since OPP09 did not show 1 kb major band but produced 0.8 kb and 1.2 kb bands in Manchu var., these three bands amplified by the primer could be considered one of the important key specifying Manchu var. related with the trait of Angelica gigas. OPC02 primer showed the same band patterns in all Korean collected lines, but not in other foreign introduced lines, such as A. sinensis from China, and A. acutiloba from Japan. Since these four RAPD primers, OPD11, OPP09, URP04, and OPC02 showed the specific polymorphisms in Angelica species, thus, these were useful to discriminate the three Angelica species, A. gigas, A. sinensis, and A. acutiloba.
Keywords
Angelica species; Manchu variety; discrimination; RAPD marker;
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