• 농업과학연구
• /
• 제49권3호
• /
• pp.655-666
• /
• 2022

We assessed the persistence of eight major cruciferous crops-leaf mustard, oilseed rape, cabbage, broccoli, cauliflower, Chinese cabbage, turnip, and radish-growing in the field. In the first part of our experiment, we tested the viability of seeds that had been buried at two different soil depths for up to 16 months. We then broadcast seeds over the soil surface and left them undisturbed to investigate the survivorship of the resultant plants over two years. Seed viability was significantly affected by plant taxa and burial depth, but not substantially affected by the duration of burial. Although seeds of leaf mustard had the greatest viability among all crops examined here, the viability rates were significantly lower at 2 cm depth than at 15 cm. Seeds of leaf mustard, oilseed rape, broccoli, turnip, and Chinese cabbage remained viable throughout the 16-month period. A study of plant demography revealed that only leaf mustard and oilseed rape succeeded in producing seeds and overwintering in the undisturbed field. However, neither of those species competed well with other plants long-term and their overall growth and survival rates declined during the evaluation period. In addition, insect herbivory severely decreased the growth of all of these crops. Our results suggest that populations of leaf mustard and oilseed rape do not tend to persist in the field for more than a few years without disturbance and external seed inputs.

• 한국초지조사료학회지
• /
• 제43권1호
• /
• pp.56-61
• /
• 2023

The germination process is critical for plant growth and development and it is largely affected by environmental stress, especially salinity. Recently, hydrogen sulfide (H₂S) is well known to act as a signaling molecule in a defense mechanism against stress conditions but poorly understood regulating seed germination. In this study, the effects of NaHS (the H₂S donor) pretreatment on various biochemical (hydrogen peroxide (H₂O₂) content and amylase and protease activity) and physiological properties (germination rate) during seed germination of oilseed rape (Brassica napus L. cv. Mosa) were examined under salt stress. The seed germination and seedling growth of oilseed rape were inhibited by NaCl treatment but it was alleviated by NaHS pretreatment. The NaCl treatment increased H₂O₂ content leading to oxidative stress, but NaHS pre-treatments maintained much lower levels of H₂O₂ in germinating seeds under salt stress. Amylase activity, a starch degradation enzyme, significantly increased over 2-fold in control, NaHS pretreatment, and NaHS pretreatment under NaCl during seed germination compared to NaCl treatment. Protease activity was highly induced in NaHS-pretreated seeds compared to NaCl treatment, accompanied by a decrease in protein content. These results indicate that NaHS pretreatment could improve seed germination under salt stress conditions by decreasing H₂O₂ accumulation and activating the degradation of protein and starch to support seedling growth.

• 한국작물학회지
• /
• 제36권5호
• /
• pp.445-458
• /
• 1991

유지작물의 안전 생산과 품질향상을 위해 지금까지 유지작물에 발생되는 주요 기상재해에 대한 국내외 문헌을 종합 고찰하였다 유지작물에 발생되는 주요 기상재해는 타작물에서와 같이 냉해나 한해와 같은 온도에 의한 장해, 습해나 도복과 같은 풍수해, 그리고 기타재해 등 각종 재해가 발생되고 있는 것으로 보고되있다. 그러나 유지작물은 국내외를 막론하고 주요작물에 비해 비중이 낮고 재배면적도 적어 비중이 낮게 취급된 관계로 각종 재해에 대한 정확한 피해정도나 재해경감 대책이 밝혀지지 많은 부분이 많은 것으로 고찰되었다. 지금까지 조사 보고된 결과들 중에서는 참깨와 땅콩에 대한 결과들이 많고 그 중에서도 침관수해, 한충해, 도복피해 등 풍수해에 대한 연구결과가 많으며 이 같은 재해들은 피해정도도 매우 큰 것으로 보고되어 심한 경우는 90%이상의 수량감소를 초래하는 것으로 보고하였다. 이와 같은 재해에 대한 피서를 최소화하기 위해서는 우선 품종개량 면에서 각종 재해에 대한 내성을 가진 품종이 개발되어야 할 것이며, 재배기술적인 측면에서도 재해를 분산시킬 수 있는 방법이나 비닐멀칭재배법 등과 같이 기상재해의 사전예방 기술도 적용되어야 할 것으로 고찰되었다. 그러나 최근의 농산물유입 개방화에 따른 다품목 소량재배 추세와 다양한 식품의 안정적 생산공급 측면에서 점차 비중이 높아가고 있는 유서 작물의 안전생산과 품질향상을 위해서는 품종개량과 재배기술 면에서 보다 적극적인 기상재해 경감대책 연구와 노력이 필요한 것으로 전망되었다.

• 한국자원식물학회지
• /
• 제19권3호
• /
• pp.440-446
• /
• 2006

To investigate flowering related genes in winter-type oilseed rape (Brassica napus L. cv. Tammi), differentially expressed genes were isolated from leaves of the plant after low temperature treatment which is requirements for floral induction. As a result of suppression subtractive hybridization (SSH), 288 clones were randomly selected from SSH library. Using reverse Northern blot analysis, 150 of 288 clones were identified to be differentially expressed. Out of these 150 clones, 45 clones showed very high identities with the known genes. Four clones showed very high identities over 90% with metallothionein-like gene that is related to flowering-induced genes. Of these 4 clones, the cDNA clone, rfs-13, revealed high identity with meotallothionein-like protein in Arabidopsis thaliana (98%) and Brassica compestris (89%). Furthermore, gene expressed in immature flower stages was confirmed by Northern blot analysis.

• The Plant Pathology Journal
• /
• 제27권2호
• /
• pp.192-192
• /
• 2011

• 한국작물학회:학술대회논문집
• /
• 한국작물학회 1991년도 추계 학술대회지
• /
• pp.20-21
• /
• 1991

• 한국작물학회:학술대회논문집
• /
• 한국작물학회 2022년도 추계학술대회
• /
• pp.21-21
• /
• 2022

• The Plant Pathology Journal
• /
• 제31권3호
• /
• pp.305-309
• /
• 2015

After four years of cold storage, dimethachlon resistance of two laboratory-induced resistant Sclerotinia sclerotiorum isolates SCG7 and LA50 declined by 99.5% and 98.9%, respectively, and cross resistance to iprodione and procymidone also declined dramatically. Along with the decline of fungicide resistance, osmotic sensitivity to sodium chloride and glucose decreased tremendously; mycelial growth rate, sclerotia number and weight per potato dextrose agar (PDA) plate increased on average by 118.6%, 85. 5% and 64.5%, respectively; and virulence to detached leaves of oilseed rape increased by 72.7% on average. Significant negative correlations were detected between dimethachlon resistance levels and mycelial growth rate on PDA (r = -0.980, P = 0.021), and between resistance levels and lesion diameters on detached leaves of oilseed rape plants (r = -0.997, P = 0.002). These results have profound implications for assessing the potential risk for resistance development to dicarboximide fungicides in S. sclerotiorum.

• BMB Reports
• /
• 제40권2호
• /
• pp.247-260
• /
• 2007

Cinnamate 4-hydroxylase (C4H) is a key enzyme of phenylpropanoid pathway, which synthesizes numerous secondary metabolites to participate in development and adaption. Two C4H isoforms, the 2192-bp BnC4H-1 and 2108-bp BnC4H-2, were cloned from oilseed rape (Brassica napus). They both have two introns and a 1518-bp open reading frame encoding a 505-amino-acid polypeptide. BnC4H-1 is 57.73 kDa with an isoelectric point of 9.11, while 57.75 kDa and 9.13 for BnC4H-2. They share only 80.6% identities on nucleotide level but 96.6% identities and 98.4% positives on protein level. Showing highest homologies to Arabidopsis thaliana C4H, they possess a conserved p450 domain and all P450-featured motifs, and are identical to typical C4Hs at substrate-recognition sites and active site residues. They are most probably associated with endoplasmic reticulum by one or both of the N- and C-terminal transmembrane helices. Phosphorylation may be a necessary post-translational modification. Their secondary structures are dominated by alpha helices and random coils. Most helices locate in the central region, while extended strands mainly distribute before and after this region. Southern blot indicated about 9 or more C4H paralogs in B. napus. In hypocotyl, cotyledon, stem, flower, bud, young- and middle-stage seed, they are co-dominantly expressed. In root and old seed, BnC4H-2 is dominant over BnC4H-1, with a reverse trend in leaf and pericarp. Paralogous C4H numbers in Brassicaceae genomes and possible roles of conserved motifs in 5' UTR and the 2nd intron are discussed.

• Journal of Applied Biological Chemistry
• /
• 제55권4호
• /
• pp.227-234
• /
• 2012

유채 종자 성숙단계별 변화하는 종자의 특성을 살펴본 결과, 개화 후 25일된 미성숙 종자에서 지방산 생성이 관찰되기 시작하였으며, 개화 후 35일된 미성숙 종자에서 지방산 생성이 거의 최고치에 달하는 것을 관찰하였으며, 이 때 백립중이 406 mg으로 가장 무거웠다. 유채 300k Microarray를 이용하여 유채 종자 성숙단계별 발현되는 유전자의 발현양상을 살펴보았다. 유채 300k Microarray는 NCBI에 등록되어 있는 543,448개의 ESTs와 780개의 cDNA정보를 군집 분석하여 80,696개의 유전자정보를 얻어 제작되었다. 개화 후 10, 25, 그리고 35일된 종자에서 total RNA를 분리하여 유채 300k Microarray 실험을 수행한 결과, 약 7,000개의 유전자에 해당하는 8.5%가 잎에 비해 종자(25DAF)에서 발현 양이 2배 이상 증가됨을 알 수 있었고, 10배 이상 증가하는 유전자 비율도 0.4%에 해당하였다. 종자 특이 발현 유전자의 발현양상을 보면, 초기에는 저장 및 세포분화 관련 유전자들의 발현 양이 높게 나타난 반면, 후기에는 지방산 대사 관련 유전자를 포함한 에너지 축적 관련 유전자들의 발현 양이 높게 나타나는 것을 관찰 할 수 있었으며, reverse transcriptase-polymerase chain reaction을 통해서 이를 확인하였다. 본 실험 결과는 종자 특이 발현 프로모터를 발굴하거나 특정 대사 기작 연구에 관여하는 유전자 발현 양상을 광범위하게 살펴봄으로써 좀 더 심도 있는 연구를 할 수 있는 기초자료를 제공하는데 많은 도움이 될 거라 사료된다.