Browse > Article

Isolation of Differentially Expressed Genes by Low Temperature Treatment in Winter Oilseed Rape (Brassica napus L. cv. Tammi)  

Chun Jong-Un (Life Resources Department, College of Agriculture and Life Science, Sunchon National University)
Seo Dong-Joong (Life Resources Department, College of Agriculture and Life Science, Sunchon National University)
Bae Chang-Hyu (Life Resources Department, College of Agriculture and Life Science, Sunchon National University)
Publication Information
Korean Journal of Plant Resources / v.19, no.3, 2006 , pp. 440-446 More about this Journal
Abstract
To investigate flowering related genes in winter-type oilseed rape (Brassica napus L. cv. Tammi), differentially expressed genes were isolated from leaves of the plant after low temperature treatment which is requirements for floral induction. As a result of suppression subtractive hybridization (SSH), 288 clones were randomly selected from SSH library. Using reverse Northern blot analysis, 150 of 288 clones were identified to be differentially expressed. Out of these 150 clones, 45 clones showed very high identities with the known genes. Four clones showed very high identities over 90% with metallothionein-like gene that is related to flowering-induced genes. Of these 4 clones, the cDNA clone, rfs-13, revealed high identity with meotallothionein-like protein in Arabidopsis thaliana (98%) and Brassica compestris (89%). Furthermore, gene expressed in immature flower stages was confirmed by Northern blot analysis.
Keywords
Brassica napus; cDNA; Flowering; Oil seed rape; Suppression subtractive hybridization (SSH); Transcription;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Goodwin, W., J.A. Pallas and G.I. Jenkins. 1996. Transcripts of a gene encoding a putative cell wall-plasma membrane linker protein are specifically cold-induced in Brassica napus. Plant Mol. Biol. 31: 771-781   DOI
2 Kawashima, I., Y. Inokuchi, M. Chino, M. Kimura and N. Shimizu. 1991. Isolation of a gene for a metallothinoein-like protein from soybean. Plant Cell Physiol. 32: 913-916
3 Kitashiba, H., T. Iwai, K. Toriyama, M. Watanabe and K. Hinata. 1996. Indentification of genes expressed in the shoot apex of Brassica campestris during floral transition. Sex Plant Reprod. 9: 186-188   DOI
4 Koornneef, M., C.Hanhart, P. van Loenen-Martinet and H. Blankestijn de vries. 1995. The effect of daylength on the transition to flowering in phytochrome-deficient, late-flowering and double mutants of Arabidopsis thaliana. Physiol. Plant. 95: 260-266   DOI
5 Levin, J.Z. and E.M. Meyerowitz. 1995. UFO: an Arabidopsis gene involved in both floral meristem identity and floral organ development. Plant Cell 7: 529-548   DOI   ScienceOn
6 Madsen, L.H., N.C. Collins, M. Rakwalska, G. Backes, N. Sandal, L. Krusell, J. Jensen, E.H. Waterman, A. Jahoor, M. Ayliffe, A.J. Pryor, P. Langridge, P. Schulze-Lefert and J. Stougaard. 2003. Barley disease resistance gene analogs of the NBS-LRR class: identification and mapping. Mol. Genet. Genomics 269: 150-161
7 Robert, L.S., F. Robson, A. Sharpe, D. Lydiate and G. Coupland. 1998. Conserved structure and function of the Arabidopsis flowering time gene CONSTANS in Brassica napus. Plant Mol. Biol. 37: 763-772   DOI   ScienceOn
8 Ryousuke, H., L. Takeshi and S.Ko. 2002. Isolation of rice genes possibly involved in the photoperiodic control of flowering by a fluorescent differential display method. Plant Cell Physiol. 43: 494-504   DOI   ScienceOn
9 Levy, Y.Y. and C. Dean. 1998. The transition to flowering. Plant Cell 10: 1973-1989   DOI   ScienceOn
10 Sgez-Vsqhez, J., P. Gallois and M. Delseny. 2000. Accumulation and molecular targeting of BnC24, a Brassica napus ribosomal protein corresponding to a mRNA accumulating in response to cold treatment. Plant Sci. 156: 35-46   DOI   ScienceOn
11 Diatchenko, L., Y.C.F. Lau, A.P. Campbell, A. Chenchik, F.Moqdam, B.Huang, S. Lukyanov,K. Lukyanov, N.Gurskaya, E.D. Sverdlov and P.D. Siebert. 1996. Suppression subtractive hybridization: A method for generation differentially regulated or tissue-specific cDNA probes and libraries. Proc. Natl.Acad. USA 93: 6025-6030
12 Baszcynski, C.L., L. Fallis and G. Bellemere. 1988. Nucleotide sequence of a full length cDNA clone of a Brassica napus ribulose- bisphosphate carboxylase-oxygenase small subunit gene. Nucleic Acids Res. 16: 4732   DOI   ScienceOn
13 Michaels, S.D. and R.M. Amasino. 2000. Memories of winter: vernalization and the competence to flower. Plant Cell and Environ. 23: 1145-1153   DOI   ScienceOn
14 Shimon G., S.Gazalah, C.Marie-Jeanne,H. Tabeb,Mizied F.O.R.N., Inbal Y., D. Chen and B. Michal. 2003. Large-scale identification of leaf senescence-associated genes. Plant J. 36: 629-642   DOI   ScienceOn
15 Kobayashi, T., E. Kobayashi, S. Sato, Y. Hotta, N. Miyajima, A. Tanaka and S. Tabata. 1994. Characterization of cDNAs induced in meiotic prophase in lilymicrosporocytes. DNA Research 1: 15-26   DOI
16 Pidkowich, M.S., J.E. Klenz and G.W. Haughn. 1999. Themaking of a flower: control of floralmeristemidentity in Arabidopsis. Trends in Plant Science 4: 64-70   DOI   ScienceOn
17 Mandel, M.A., C. Gustafson-Brown, B. Savidge and M.F. Yanofsky. 1992.Molecular characterization of Arabidopsis floral homoeotic gene APETALA1. Nature 360: 273-277   DOI   ScienceOn
18 Blazquez, M.A., L.N. Soowal, I. Lee and D. Weigel. 1997. LEAFY expression and flower initiation in Arabidopsis. Development 124: 3835-3844
19 Ok, S.H., S.H. Shin, J.S. Shin, K.M. Kim and J.U. Chun. 2004. Isolation of genes related to light and lowtemperature stress in barley (Hordeum vulgare L.). Kor. J. Breed. 36: 38-46
20 Ok, S.H.,H.M. Park, Y.J Kim., S.C. Bahn, J.M. Bae, M.C. Suh, J.U. Jeung, K.M. Kim and J.S. Shin. 2003. Identification of differentially expressed genes during flower development in carnation (Dianthus caryophyllus). Plant Sci. 165: 291-297   DOI   ScienceOn
21 Kole, C., P. Quijada, S.D. Michaels and R.M. Amasino. 2001. Evidence for homology of flowering-time genes VFR2 from Brassica rapa and FLC from Arabidopsis thaliana. Theor. Appl. Genet. 102: 425-430   DOI
22 Hitz, W.D., T.J. Carlson, J.R. Booth Jr, A.J. Kinney, K.L. Stecca and N.S.Yadav. 1994. Cloning of a higher-plant plastied omega-6 fatty acid desaturase cDNA and its expression in a Cyanobacterium. Plant Physiol. 105: 635-641   DOI
23 Zhou, J. and P.B. Glodsbrough. 1995. Structure. organization and expression of the metallothionein gene family in Arabidopsis. Mol. Gen. Genet. 248: 318-328   DOI
24 Blazquez, M.A., R. Green, O. Nilsson, M. Sussman and D. Weigel. 1998. Gibberellins promote flowering of Arabidopsis by activating the LEAFY promoter. Plant Cell 10: 791-800   DOI   ScienceOn
25 Lardon, A. and A.M. Triboi-Blondel. 1995. Cold and freeze at flowering effects on seed yields inwinter rapeseed. Field Crops Research 44: 95-101   DOI   ScienceOn