• Journal of Life Science
• /
• v.32 no.12
• /
• pp.997-1004
• /
• 2022

To verify the nutritional superiority and safety of Protaetia brevitarsis larvae fed a food source containing soybean curd cake, the nutrients and harmful substances of larvae reared only with fermented oak sawdust and a food source containing soybean curd cake were comparatively analyzed. The crude protein content was 50.0% in the larvae fed with a food source containing soybean curd cake (SCC), which was 1.1 times higher than that of the larvae fed with fermented oak sawdust (FOS) at 44.7%. Potassium, which showed the highest content among macrominerals, was 2.5 times higher in SCC (3,169.6 mg/100 g) than in FOS (1,245.9 mg/100 g). Among trace minerals, zinc showed 12.6 times higher content than FOS (8.2 mg/100 g) in SCC (103.0 mg/100 g). As a result of the analysis of harmful substances, all heavy metals met the edible insect control standards, and pathogenic microorganisms, such as E. coli and Salmonella spp, were not detected. According to the above study, the larvae of P. brevitarsis fed soybean curd cake contain various nutrients, and safety has also been confirmed, so it is judged to be suitable for use as food.

• The Korean Journal of Mycology
• /
• v.50 no.2
• /
• pp.93-102
• /
• 2022

A new cultivar of oak mushroom Lentinula edodes 'Jadam' was bred from monokaryotic strains of 'Hwadam-18' and 'GMLE36295-22'. The optimum temperature for mycelial growth of 'Jadam' on potato dextrose agar was 22-25℃. Total cultivation period of the new cultivar, from inoculation to its first harvest, was 122-124 days, shorter to that of the control cultivar 'Hwadam'. Total yield of 'Jadam' was 623.8 g per 3 kg substrate, and is higher than that of 'Hwadam' (455.2 g). In the fruiting body of the new cultivar, the stipe was shorter and thinner than those of 'Hwadam'. A farmhouse field test showed that the period of mycelial growth and browning was shorter than that of control cultivar 'L808'. The total yield for 2 flush was 543.3 g for 'Jadam'. It was similar to the 585.3 g of the control cultivar. Therefore, the new cultivar 'Jadam' could be a substitute for 'L808' in the field of farms.

• Journal of Mushroom
• /
• v.16 no.2
• /
• pp.70-73
• /
• 2018

To determine the optimum amount of spent oyster mushroom substrate (SOMS) for use in cultivation of Lentinula edodes, the chemical properties of the substrate and culture conditions of Lentinula edodes were investigated. Replacing 20-50% of a sawdust substrate with SOMS yielded a C/N ratio of 62-76. In case of substrates containing SOMS, the total nitrogen and phenolic contents of were higher, whereas fructose and organic acid contents were lower than those of the control substrate. Cultivation tests showed that the 3-cycle yield of 20% SOMS treatment was 286.7 g, similar to that of the control, while 50% SOMS treatment significantly decreased the yield. In conclusion, development of oak mushroom substrate using SOMS would recycle waste products and decrease material costs.

• Journal of Mushroom
• /
• v.13 no.4
• /
• pp.294-300
• /
• 2015

We cultivated 117 hybrid strains of Lentinula edodes in greenhouse. 5 hybrid strains(07-55, 07-66, 07-84, 07-93 and 07-117) were selected from comparison of their characteristics of fruiting body and productivity. Biological efficiency of 5 hybrid strains was investigated 36.1(07-66), 35.6(07-117), 27.1(07-93), 25.7(07-84) and 6.5%(07-55), respectively. Selected strains 07-66, 07-117 and 07-93 showed high biological efficiency but observed commercially low quality fruiting body. Temperature type of fruiting body of 5 hybrid strains observed as follows high temperature type(07-117), mid temperature type(07-66 and 07-84) and low temperature type(07-55 and 07-93). Temperature type of fruiting body of 07-93 strain has wide range relatively. In the results, 07-84 strain was selected finally with consideration for quality of fruiting body, productivity and temperature type($8-21^{\circ}C$) in farm test.

• Journal of Mushroom
• /
• v.6 no.1
• /
• pp.20-26
• /
• 2008

The stromatal forms of T. fuciformis and the mycelia of Hypoxylon sp. were collected. The DNA sequence in the ITS region of the 5.8S ribosomal genes of isolated strain KG103 was very similar to that of T. fuciformis AF042409 with a homology of over 98% in the EMBL/GenBank database through BLAST searching. A second isolate, No KG201, one of the symbiotic strains for cultivating T. fuciformis also exhibited high homology with Annulohhypoxylon stygium AJ390406. Potato Dextrose Medium exhibited the best mycelial growth of 14 mm/14 days and 85 mm/14 days for T. fuciformis and its symbiotic fungi, respectively. Optimum culture conditions for the micelial growth were pH 5 at $25^{\circ}C$. For the optimization of artificial cultivation of T. fuciformis in bottle with sawdust medium, several conditions such as type of sawdust, supplements, pH, moisture content, and incubation temperature were investigated. T. fuciformis and symbiotic fungi showed fast mycelial growth on corn cob media (77 and 52%) followed by oak tree sawdust and cotton seed meal. The optimal temperature for mycelial growth of T. fuciformis and symbiotic fungi on corn cob media was $25^{\circ}C$ at 55% of moisture content.

• The Korean Journal of Mycology
• /
• v.9 no.3
• /
• pp.123-128
• /
• 1981

Three edible species, e.g. Lyophyllum sp. and Armillaria mellea introduced from Japan in 1979 and Auriaularia auricula-judae from Taiwan in 1978 were selected for artifcial cultivation. The media, MSDS for Lyophyllum sp, MHS and PDS for A. auricula-judae, and MSDS and MHS for A. mellea were selected. The range of optimum pH of Lyophyllum sp. was 6.0 and pH 8.0 for A. auricula-judae and pH 6.0 to 7.0 for A. mellea. The range of optimum temperature for the mycelial growth of Lyophyllum sp. was 25 to $30^{\circ}C,\;30^{\circ}C$ for A. auricula-judae and $25^{\circ}C$ for A. mellea. When the moisture content 75 to 80% (w/w) in the substrates, the mycelial growth of Lyophyllum sp. and A. auricula-judae, was rapid. 65% (w/w) for A. mellea. As the spawning substrates, wheat for Lyophyllum sp. oak sawdust for A. auricula-judae and poplar sawdust for A. mellea were selected, respectively. In this experiment, yields of Lyophyllum sp. and A. auricula-judae were high on the poplar sawdust added 20% (w/w) of rice bran. Lyophyllum sp. and A. auricula-judae for sources of new edible mushrooms seems to be possible.

• Journal of Mushroom
• /
• v.5 no.1
• /
• pp.29-33
• /
• 2007

This study for establishment of cultivation technique was carried out in home, Pleurotus ostreatus, Ganoderma lucidum. The mushroom kit allows you to grow some of the mushrooms used by the finest chefs in the world easily and quickly almost anywhere in your own home or office. Mushroom kits may be placed on a coffee table, counter, or desk. They will produce mushrooms virtually anywhere room temperature is maintained. Now we have the opportunity not only to enjoy watching these exotic mushrooms grow but also to enjoy eating the freshest organic mushrooms possible. To study for the possibility of indoor cultivation of P. ostreatus, and G. lucidum, we invested cultivation status with two kinds of sawdust, cotton waste and rice straw in washroom, kitchen, living room, and bedroom. It took 2~4 days to make primordia formation in a block of P. ostreatus, and G. lucidum was 11 days. Weight of P. ostreatus on cotton waste, rice straw, and willow sawdust were 2,060g, 90g, and 770g, respectively. and weight of G. lucidum on oak sawdust was 172g. Th best result was achieved in washroom, among used washroom, kitchen, living room, and bedroom.

• Journal of Korea Foresty Energy
• /
• v.23 no.2
• /
• pp.21-28
• /
• 2004

Three different strains of Lentinula edodes, Sanlim 5-Ho, Sanlim 6-Ho and Nongki 3-Ho, were cultured in the sawdust media of Mongolian oak(Quercu mongolica Fisch) for 90 days under dark and light conditions(each 30 days) and fruiting period(30 days). Weight loss of sawdust media was determined after fungal cultures and the contents of ergosterol in fungal mycelia were quantified by HPLC analysis followed by solvent extraction. Compared with the two other fungal strains$(8\%)$, weight loss of Sanlim 5-Ho was slightly lowered to $7\%$. The level of ergosterol content, a parameter for fungal growth, was continuously enhanced in Sanlim 5-Ho for dark and light incubation periods. However, Sanlim 6-Ho and Nongki 3-Ho recorded the maximized fungal growth under light condition. In fruiting periods the ergosterol contents were lowered in the three strains. Intra- and extracellular enzymes during cultural and fruiting periods were also characterized. The activity of Mn-peroxidase and laccase, which are characteristics enzymes for white rot fungi as lignin degrading enzymes, were determined as a high level overall the periods. As cellulose degrading indicators, the activity of CMCase, avicelase, xylanase and glucanase were detectable in initial incubation period.

• Korean journal of applied entomology
• /
• v.44 no.3 s.140
• /
• pp.189-197
• /
• 2005

Cellulose-digesting bacteria were isolated from hindgut of Allomyrina dichotoma (Coleoptera: Dynastidae). The bacterial isolates were identified as Yersinia sp. and Bacillus sp. The addition of the identified bacteria to diet increased growth rate of the cetoniid beetle, Protaetia brevitarsis senlensis (Coleoptera: Cetoniidae), probably by digesting cellulose nutrient contained in the oak tree sawdust diet. An additive of wheat flour at more than 10% to the sawdust diet significantly enhanced growth of P. brevitarsis senlensis. Trimmed branches of apple trees have been disposed in the apple farms and could be used for a diet component of the cetoniid beetle when the cellulose-digesting bacteria were mixed with the derived-sawdust. Resulting manure from mass rearing of P. brevitarsis senlensis contained high organic matters and trace amounts of toxic metals. When the manure were splayed on soil, it was effective as a natural compost and significantly stimulated lettuce growth. This research suggests a model technology to use cellulose-digesting bacteria to use for culturing grub, which results in natural recycles of trimmed branches in apple farms as grub diet, and to use grub manure as a natural compost.

• Mycobiology
• /
• v.36 no.2
• /
• pp.88-92
• /
• 2008

Vegetative growth of four different strains of Hericium erinaceus was observed. The temperature suitable for optimal mycelial growth was determined to be $25^{\circ}C$, with growth observed in the extend temperature range of $20{\sim}30^{\circ}C$. The different strains of this mushroom showed distinct pH requirements for their optimum vegetative growth, with the most favorable growth observed at pH 6. Considering vegetative mycelial growth, PDA, YM, Hennerberg, Hamada, and Glucose peptone were the most favorable media, and Czapek Dox, Hoppkins, Glucose tryptone, and Lilly were the most unfavorable media for these mushroom strains. With the exception of lactose, most of the carbon sources assayed demonstrated favorable vegetative growth of H. erinaceus. For mycelial growth, the most suitable nitrogen source was alanine and the most unsuitable was histidine. Oak sawdust medium supplemented with $10{\sim}20%$ rice bran was the best for mycelial growth of the mushroom.