• Journal of Ginseng Research
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• v.44 no.4
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• pp.544-551
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• 2020

Background: Previous studies have shown the insecticidal efficacy of ginsenosides. In the present study, we aimed to investigate the metabolic mechanism related to the inhibitory effect of panaxadiol saponins (PDSs) against the Asian corn borer Ostrinia furnacalis (Guenee). Methods: Third instar larvae of O. furnacalis were fed normal diets with different concentrations of PDSs for 4 days. The consumption index, relative growth rate, approximate digestibility, and conversion of ingested and digested food were recorded. A targeted gas chromatographye-mass spectrometry assay was performed to detect the profiles of amino acids, fatty acids, and carbohydrates in larvae of O. furnacalis. In addition, the activity of detoxification-related enzymes was determined. Results and Conclusions: PDSs decreased the consumption index, relative growth rate, approximate digestibility, and conversion of ingested and digested food in the 3rd instar larvae of O. furnacalis in a dose-dependent manner. PDSs decreased 15 free amino acids, 16 free fatty acids, and 5 carbohydrates and increased the levels of palmitoleic acid, palmitic acid, and 9-octadecenoic acid in the 3rd instar larvae. The activity of detoxification-related enzymes, such as acetylcholinesterase, glutathione S-transferase, cytochrome P450, carboxylesterase, trehalase, acid phosphatase, and alkaline phosphatase, was reduced in a dose-dependent manner in the 3rd instar larvae exposed to PDSs. These data confirmed the inhibitory effect of PDSs against growth, food utilization, and detoxification in the 3rd instar larvae of O. furnacalis and the potential for using PDSs as an efficient tool for insect pest management for O. furnacalis larvae.

• Korean journal of applied entomology
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• v.32 no.4
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• pp.395-406
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• 1993

This experiment was carried out to develop an artificial diet and a mass rearing system, which is essentIal in the pheromone research for Ostrmia furnacalis. Component deletion and addit.ion methods were applied t.o define the nut.ritional Importance of some components, especially yeast, Wesson's salt mixture and vitamin complex. Secondly we have developed a proper rearmg syst.em for O. furnacalis. and also tried to find out the factors affecting rapid sclerotization (dark-canning) of pupae. AddItion of yeast raIsed the pupal weight. and pupation rate. However Wesson's salt mixture got the female pupae lighter. Vitamin complex as a substitue for yeast showed a good survivahty in an early larval stage buL pupal weight was lower than that of the individuals reared on the yeast dIet. Also corrugated cardboard was found to be very good for pupation. And the rate of dark~tannmg in pupae was higher as the period of larval development was shorter.

• Korean journal of applied entomology
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• v.61 no.1
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• pp.275-281
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• 2022

We investigated the occurrence pattern of the Asian Corn Borer, Ostrinia furnacalis in job's tears, and we tested the field control effects of 5 insecticides and 3 eco-friendly materials. Adults of O. furnacalis began to occur in May and have passed three generations until the end of October. As results of the field test, five insecticides (novaluron, lufenuron, spinetoram, cyclaniliprole, flufenoxuron) and three eco-friendly materials (gosam extracts 90%, neem extracts 60%, citronella oil 30 + derris extracts 20 + cinnamon extracts 10%) showed high control effects against O. furnacalis in two different areas, and no phytotoxicity even at double dose of pesticides.

• Korean journal of applied entomology
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• v.55 no.4
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• pp.329-336
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• 2016

Occurrence of oriental corn borer, Ostrinia furnacalis, and yield in a paddy-upland rotation field for 8 maize (Eolrukchal 1, Heugjeom 2, Miheukchal, Ilmichal, Heukjinjuchal, Chalok 4, Mibaek 2, Daehakchal) and 7 sorghum (Hwanggeumchal, Anzunbaengisusu, Moktaksusu, Sodamchal, DS-202, Nampungchal, Donganme) varieties has been surveyed. In a monitoring study using a pheromone trap carried out from 15 May to 10 September, the density of O. furnacalis adults increased rapidly from about 2 weeks after maize planting and reached the highest density at mid June. After that, their density was fluctuated a little at earlier September. The number of the damaged maize and invasive pores on the stem of 2 maizes and sorghum varieties was examined. The mean number of the damaged maize per 20 plants was 19 and 18 plants, and the number of invasive pores was 1.8 and 1.4 per maize stem in Daehakchal and Mibaek 2, respectively. In a survey carried out at harvest period using 8 maize varieties, the damaged ratio was 94%, 92%, 71%, 64%, 54%, 52%, and 45% in Daehakchal, Mibaek 2, Ilmichal, Eolrukchal 1, Chalok 4, Miheukchal, and Heugjeom 2, respectively. The number of invasive pore per Ilmichal stem was 1.4 and that of the others was less than 1.0 per stem. In addition, the damaged ratio of maize ears was 50% in Ilmichal, 40% in Heukjinjuchal, 37% in Daehakchal, etc. The damage pattern of 2 sorghum varieties, Nampungchal and Donganme, by O. furnacalis larvae was steeply increased from planting to 2 months and the trend was continued up to earlier August. At this time, the mean number of damaged sorghum was 13 and 9.2 plants for Nampungchal and Donganme, and the number of invasive stem pores was 1.06 and 0.46, respectively. In another survey carried out at harvest period for 7 sorghum varieties, their damaged ratio was 95% in DS-202, 76% in Moktaksusu, 75% in Sodamchal, 67% in Nampungchal, 57% in Anzunbaengisusu, 46% in Donganme, and 34% in Hwanggeumchal. The damage of sorghum varieties was much higher and severer than that of maizes by O. furnacalis larvae. The number of invasive pores on a sorghum stem was 1.7 in DS-202, 1.4 in Moktaksusu, 1.3 in Sodamchal, 1.1 in Nampungchal, 1.0 in Anzunbaengisusu, 0.5 in Donganme, and 0.4 in Hwanggeumchal. Meanwhile, there was no distinct connection between damaging results and yields of maizes and sorghums by O. furnacalis larvae in a paddy-upland rotation field. These results from this study can be applicable for the establishment of a management strategy to control Oriental corn borer in paddy-upland rotation fields for maize and sorghum.

• Korean Journal of Organic Agriculture
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• v.26 no.3
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• pp.409-425
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• 2018

Occurrence and yield characteristics of lepidopteran insect pests in both PUR (paddy-upland rotation) and CU (continuing upland) fields for 8 maize (Eolrukchal 1, Heugjeom 2, Miheukchal, Ilmichal, Heukjinjuchal, Chalok 4, Mibaek 2, Daehakchal) and 7 sorghum (Hwanggeumchal, Anzunbaengisusu, Moktaksusu, Sodamchal, DS-202, Nampungchal, Donganme) varieties was surveyed. The population of Ostrinia furnacalis adults showed 3 times highest peaks in 2016. In addition, the damaged ratios of maize and sorghum leaves and stems were increased depending on the growth periods of the crops and those by O. furnacalis larvae were also increased steeply in mid August. The damaged ratios of maizes such as Miheukchal, Eolrukchal 1, Ilmichal, Heugjeom 2, and Mibaek 2 by O. furnacalis larvae were statistically different (P=0.05) between organically managed PUR and CU fields. Except for DS202 among sorghum varieties, the damaged ratios of the other ones were high and similar (50-92%) in the CU field. The ear damaged ratio of maize varieties by lepidopteran larvae including O. furnacalis and Helicoverpa armigera was from 5 to 38% in Chalok 4 and Heukjinjuchal, respectively, and Daehakchal gave relatively higher damage ratio in the PUR field (33%) than in the CU field (14%). The ear ratio of Sodamchal and DS-202 varieties in the PUR field was 94 and 82%, and all sorghum varieties were damaged over 78% by lepidopteran larvae in the CU field. Interestingly, the correlation coefficients between the damaged ratio and invasive pores on their stems by O. furnacalis larvae were positive (maize $r=0.69_{paddy-upland}$, $0.95_{continuing}$; sorghum $r=0.93_{paddy-upland}$, $0.97_{continuing}$, respectively). Additionally, the correlation coefficients between the damaged ratio and the ear weight or the thousand kernel weight (g) of maizes and sorghums by lepidopteran larvae were negative (maize $r=-0.38_{paddy-upland}$, $-0.49_{continuing}$; sorghum $r=-0.70_{paddy-upland}$, $-0.57_{continuing}$, respectively). These results indicate that the focused management on lepidopteran insect pests occurring maizes and sorghums cultivated in PUR and CU fields is an important strategy to secure their yields.

• Korean journal of applied entomology
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• v.60 no.4
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• pp.449-462
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• 2021

We analyzed the effects of temperature on the survival, development, and reproduction of the non-diapause Asian corn borer, Ostrinia furnacalis (Lepidoptera: Crambidae). A 16:8 h light:dark photoperiod was supplied to the non-diapause stages of O. furnacalis. The insects were raised on an artificial diet at seven constant temperatures between 15 and 35℃ for immature stages, and eight temperatures between 13 and 33℃ for the adult stage. The survival rate of eggs exceeded 70% at all temperatures, whereas survival of larvae was as low as 7.4% at 15℃. The developmental periods of the immature stages decreased with increasing temperature, but the larval period did not decrease further at 35℃. The pupal weight increased with increasing temperature, but the female weight decreased at 35℃. Variations with different last larval instars within the colonies at different temperatures were observed. Adults produced offspring at all tested temperatures. Adult longevity and the pre-oviposition and oviposition periods decreased with increasing temperature, but the pre-oviposition period increased at 33℃. Total fecundity exceeded 400 viable eggs at 22℃ and 31℃. The mean daily fecundity during the oviposition period and the mean daily fecundity on the day of oviposition increased with increasing temperature, but decreased at 33℃. Daily fecundity sharply increased at earlier adult ages and slowly decreased thereafter. The simulated oviposition frequency was greatest at 22℃. The lower developmental threshold temperatures were estimated to be the lowest at 9.7℃ for the first instar larva and the highest at 14.7℃ for the 5th to last instar larval stage, using a linear model.

• Korean journal of applied entomology
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• v.14 no.4 s.25
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• pp.221-225
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• 1975

The European corn borer, Ostrinia nubilalis (Hubner) has been known as only one species of genus Ostrinia in Korea. After Mutuura and Munroe(1970) revised the genus Ostrinia, the name of the corn stem borer in Korea has been very confused. The critical examination of the external morphology and the genitalia revealed that species of the genus Ostrinia occurring in Korea are O. fyrbacakus (Guenee, O. scapulalis (Walker), O. zaguliaevi Mut. & Mun. and O. zealis (Guenee)

• Korean journal of applied entomology
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• v.60 no.4
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• pp.431-448
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• 2021

We decided the efficient application timings of organo-synthetic insecticides for controlling the first generation larvae of O. furnacalis through investigations of insect stage-specific densities, damage aspects in maize, and effects of insecticides. A waxy maize cultivar, Ilmichal, was cultivated from April 20 (sowing) to July 26 (harvest, dough stage of maize) in Suwon, 2016. The maximum and 50% cumulative catch dates of the overwintering generation adults in the sex pheromone trapping were May 29 and May 31, respectively. Most of the first generation larvae finished their occurrence till the early reproductive stage of maize. The first generation larvae fed on leaves inside the whorl leaves before tassel and stem development of maize, sequentially moved to tassel and stem, and then moved finally to stem and ear parts. In the results of insecticide applications at different dates, the 9-11 leaf stage (June 10~17) and the 6-7 leaf stage (June 3) of maize were the most efficient application timings for direct spray of Etofenprox EC to maize, and for application of Carbofuran granules onto soil surface, respectively, which resulted in suppression of tunnelling damages. The timings for the two insecticides were 12-19 days and 5 days after the adult maximum catch date, respectively. Those timings after the 50% cumulative adult catch date were advanced 2 days.

• Korean journal of applied entomology
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• v.59 no.3
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• pp.185-202
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• 2020

The diapause induction season in Ostrinia furnacalis (Lepidoptera: Crambidae) was estimated in Suwon. Three batches of adult generations were observed, the first one from early May to early July, the second from early or mid-July to early or mid-August, and the third from mid-August to October. In outdoor larval rearing, colony rearing occurring from mid-July to mid-August produced both non-overwintering and overwintering larvae, whereas late-reared colonies produced only overwintering larvae. Larvae collected during July and August in maize fields produced both non-overwintering and overwintering larvae, whereas late-collected larvae produced only overwintering larvae. The results indicated that O. furnacalis has a bi- or trivoltine complex life cycle in this area. In the laboratory, when larvae of all instars within 9 h after molting were first treated to a diapause induction condition (11:13 h = light:dark photoperiod and 20℃), almost all larvae were induced to diapause. However, when similar treatments were conducted age-specifically for the 5th instar larvae, diapause induction rates in 3- and 4-day-old larvae of the 5th instar decreased. In contrast, when larvae were subjected to the diapause induction treatment only during the periods from the hatching stage to the 2nd, 3rd, and 4th instar, almost all larvae were not induced to diapause. The results suggest that the early age of the 5th larval instar is the last stage for sensitivity to diapause induction stimuli. In the diapause-induced larvae, hemolymph trehalose content increased and body supercooling points dropped, compared with those in non-diapause larvae.