• Journal of Animal Science and Technology
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• v.45 no.2
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• pp.211-218
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• 2003

For the Exp. 1, a total of sixty pigs (10.57$\pm$0.30kg average initial body weight) were used in a 15-d growth assay to determine the effect of dietary $\alpha$-1,6-galactosidase and $\beta$-1,4-mannanase on growth performance and nutrient digestibility. Dietary treatments included 1) CON (corn-dried whey-SBM based diet), 2) EC0.1 (CON diet+0.1% enzyme complex of $\alpha$-1,6-galactosidase and $\beta$-1,4-mannanase). Through the entire experimental period, gain/feed of pigs fed EC0.1 diet was higher (0.43 vs 0.52) than that of pigs fed CON diet (P<0.05). Pigs fed EC0.1 diet showed significant (P<0.05) improvement in dry matter (74.82% vs 82.41%) and nitrogen (70.59% vs 77.88%) digestibilities compared to pigs fed CON diet. For the Exp. 2, a total of thirty six pigs (22.30$\pm$0.45kg average initial body weight) were used in a 30-d growth assay to determine the effects of dietary $\alpha$-1,6-galactosidase and $\beta$-1,4-mannanase in low energy diet on growth performance and nutrient digestibility. Dietary treatments included 1) AME (adequate ME diet), 2) AME+EC0.1 (AME diet+0.1% enzyme complex) and LME+EC0.1 (low ME diet + 0.1% enzyme complex). Through the entire experimental period, average daily feed intake of pigs fed enzyme complex supplemented diets was higher than that of pigs fed CON diet (P<0.05). Also, pigs fed AME+EC0.1 diet showed significant (P<0.05) increase in ADFI (1,401g vs 1,733g) compared to pigs fed CON diet. Pigs fed enzyme complex supplemented diet showed significant (P<0.05) improvement in dry matter and nitrogen digestibilities compared to pigs fed CON diet. In conclusion, the results obtained from these feeding trials suggest that the supplementation of $\alpha$-1,6-galactosidase and $\beta$-1,4-mannanase was an effective means for improving growth performance and dry matter and nitrogen digestibilities in nursery and growing pigs.

• Korean Journal of Poultry Science
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• v.34 no.4
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• pp.259-269
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• 2007

This study was compared the effect of shelf-life and DHA accumulation in chicken meat from broilers fed experimental diets for two weeks($21{\sim}35$ days) of growers. Two hundred-ten male Ross broilers, 1 day of age, were randomly allocated to seven treatment groups. Experimental diets were assigned to each of the seven groups: control diet containing tallow, T1 with 1.00% fish oil, T2 with 2.00% fish oil, T3 with 2.00% fish oil, 200 ppm vitamin E and 200 ppm vitamin C, T4 with 2.00% fish oil and 200 ppm vitamin C, T5 with 2.00% fish oil and 200 ppm vitamin E, and T6 with 3.00% fish oil. The levels of DHA in chicken meat was the highest in T6, and T1 in breast muscle and thigh muscle with skin was higher than that of $T2{\sim}T5,\;T2{\sim}T5$ in raw chicken meat and wing with skin was about two-fold higher than that of T1. The contents of DHA in chicken meat according to storage days were significantly reduced to 42.30%, 49.38% and 48.51% in T1, T2 and T6, respectively, and this decrease was higher than that of T3, T4 and T5 (p<0.05). Particularly, the rate of reduction of DHA was the lowest in the T3 and T5, which were the lowest in TBARS(thiobarbituric acid reactive substances). TBARS increased in the order of T6, T2 and T1, but reduced in the order of T3, T5 and T4 according to storage days, and there was a significant difference among the treatment groups (p<0.05).

• Journal of Animal Science and Technology
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• v.46 no.1
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• pp.49-54
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• 2004

Effects of low quality roughage sources on duration and frequencies of rumination and chewing in Hanwoo steers were determined, Animals were fed three diets; concentrate+rice straw(SO:SO), concentrate+ rice straw + popped rice hull(SO:3S: IS) and concentrate+ rice straw+ground rice hull(SO:3S: IS) to compare both rice straw alone and combination with rice hull. Eating and ruminating time of steers fed concentrate+ rice straw(SO:50), concentrate+ rice straw + popped rice hull(50:35:15) and concentrate + rice straw + ground rice hull(50:3S:15) were 78.8 and 338.4minlday; 98.0 and 362.5minlday, and 160 and 519.2min/day, respectively. When steers were fed popped rice hull and ground rice hull, time spent for both eating and ruminating was significantly increased(p <0.05). When steers fed popped and ground rice hull, number of ruminating chews and number of chews per rumination time were significantly decreased(p<0.05). The number of rumination boli and boli per rumination time had significantly decreased(p<0.05), but chewing time per boli, number of chew'S per bolus were significantly increased(p

• Journal of the Korean Crystal Growth and Crystal Technology
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• v.15 no.6
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• pp.244-251
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• 2005

The relationship between particle size of hydro-thermally synthesized barium titanate powders (BT01, BT02, BT03, BT04, BT05) and the powder properties was investigated by means of particle size, specific surface area, zeta potential, XPS, XRD and SEM. Particle size determined by laser light scattering is closely related with specific surface area and the tetragonality (c/a) obtained from XRD. The specific surface area of the samples inversely decreased with increasing particle size except BT03 powder. BT03 sample showed higher surface area than BT04 sample of equivalent particle size, which was attributed mostly to the agglomeration of particles in terms of SEM image and XRD analysis. Zeta potential increased with increasing particle size with the exception of BT02 and BT03 which showed larger minus value of zeta potential in comparison with other BT powders. Beta potential results of BT02 and BT03 are considered to be related with the dissolution of $Ba^{2+}$ ion in these powers which was examined by XPS.

• BMB Reports
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• v.29 no.3
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• pp.230-235
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• 1996

The interaction of ${\alpha}-ketoglutarate$ dehydrogenase complex (${\alpha}-KGDC$) with a hydrophobic fluorescent probe [1,1'-bi(4-aniline)naphthalene-5,5'-disulfonic acid] (bis-ANS) was studied. The punfied ${\alpha}-KGDC$ was potently inhibited by bis-ANS with an apparent half maximal inhibitory concentration ($IC_{50}$) of 9.8 ${\mu}m$ at pH 8.0. The catalytic activities of both the E1o and E2o subunits were predominantly inhibited while that of the E3 component was hardly affected. The binding of bis-ANS to the enzyme caused a marked enhancement and blue shift from 523 nm to 482 nm in the fluorescence emission spectrum. The dissociation constant ($K_d$) and the number of binding sites (n) were calculated to be 0.87 mM and 158, respectively. Allosteric regulators such as purine nucleotides and divalent cations further increased the fluorescence intensity of the $bis-ANS-{\alpha}-KGDC$ binary complex. These data suggest that the binding of these allosteric regulators to ${\alpha}-KGDC$ may cause the conformational changes in the enzyme and that bis-ANS could be used as a valuable probe to study the interaction of the multi-enzyme complex and its allosteric regulators.

• The Korean Journal of Soil Zoology
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• v.5 no.2
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• pp.63-70
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• 2000

The effect of combinations of temperature, water column height and extraction time onthe efficiency of wet funnel extraction methods for enumerating Enchytraeids in a blanket bog peat soil from Moor House, Cumbria, England were investigated. The optimal conditions for extracting enchytraeids from this study were found to be a water surface temperature of 35$^{\circ}C$, with an extraction time of 6 hours, regardless of initial water temperature and water level in the extraction funnel. The original O'Connor method (40-45$^{\circ}C$ for 3 hours extraction and gradually increasing heating) yielding a high variation in the extraction efficiency, largely due to the comparatively higher temperature and shorter extraction time than this method. Attempts to extract without heat over longer periods showed very low extraction efficiencies for this highly organic blanket bog soil when compared with any of the heating wet funnel methods.

• Safety and Health at Work
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• v.7 no.3
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• pp.218-224
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• 2016

Background: Butchering is often associated with high rates of work-related musculoskeletal disorders (WRMSDs). However, published work on the prevalence of WRMSDs among butchers in Nigeria is scarce. This is important because meat processing practices differ across geographical and cultural locations. This study was therefore aimed at analyzing WRMSDs among butchers in Kano metropolis. Methods: Sociodemographic and work-settings information was obtained from 102 male cattle butchers (age, $37.49{\pm}11.68years$) through survey. Information on the prevalence and pattern of musculoskeletal disorders was obtained from the respondents using the Standardized Nordic Questionnaire. Additional information on health seeking practices was also obtained using a pro forma. Associations between the prevalence of WRMSDs and each of the sociodemographic data and work settings were explored using Chi-square analysis. The level of significance was set at p < 0.05. Results: The 12-month and point prevalence rates of WRMSDs among butchers in this study were 88.2% and 74.5%, respectively. Whereas lower back complaints (66.7%) were the overall and lower body quadrant's most commonly reported WRMSDs among the butchers surveyed, wrist/hand complaints were the leading upper quadrant's (45.1%) most commonly reported WRMSDs among the respondents. There were significant associations between age and majority of WRMSDs in the body regions. Only 23.3% of the 90 individuals who had WRMSD visited the hospital to seek redress for their WRMSD. Conclusion: The prevalence of WRMSDs is high among butchers in Kano Metropolis. Few individuals with WRMSD utilize healthcare facilities. Age is a major risk factor in this setting.

• Journal of the Korean Ceramic Society
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• v.56 no.4
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• pp.412-420
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• 2019

In this study, the dielectric and polarization properties of manganese (Mn% = 0.0, 0.1, 0.2, 0.5) doped (Pb_0.93La_0.07)(Zr_0.82Ti_0.18)O₃ (PLZT 7/82/18) anti-ferroelectric ceramics were studied for energy storage capacitor and pyroelectric applications. A systematic investigation demonstrated that the electric properties of PLZT 7/82/18 ceramics are affected significantly by the Mn-doping content. A maximum dielectric constant of ~ 2,128 at 1 kHz was found for 0.1% Mn-doped PLZT ceramics with a low dielectric loss of 0.018. The bipolar polarization versus electric field (P-E) hysteresis loops were traced for all compositions showing a typical anti-ferroelectric nature. The breakdown field was found to decrease with Mn-doping. The energy storage density and efficiency were found to be 460 J/㎤ and ~ 63%, respectively, for 0.2% Mn-doped PLZT ceramics. The pyroelectric coefficient of PLZT ceramics shows an increase based on the amount of Mn-doping.

• Nuclear Engineering and Technology
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• v.55 no.5
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• pp.1519-1526
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• 2023

For the first time Aluminium-BariumeZinc oxide nanocomposite (ZABONC) was synthesized by solution combustion method where calcination was carried out at low temperatures (600℃) to study the electromagnetic (EM) (X/γ) radiation shielding properties. Further for characterization purpose standard techniques like PXRD, SEM, UV-VISIBLE, FTIR were used to find phase purity, functional groups, surface morphology, and to do structural analysis and energy band gap determination. The PXRD pattern shows (hkl) planes corresponding to spinel cubic phase of ZnAl₂O4, cubic Ba(NO₃)₂, α and γ phase of Al₂O₃ which clearly confirms the formation of complex nano composite. From SEM histogram mean size of nano particles was calculated and is in the order of 17 nm. Wood and Tauc's relation direct energy band gap calculation gives energy gap of 2.9 eV. In addition, EM (X/γ) shielding properties were measured and compared with the theoretical ones using standard procedures (NaI (Tl) detector and multi channel analyzer MCA). For energy above 356 keV the measured shielding parameters agree well with the theory, while below this value slight deviation is observed, due to the influence of atomic/crystallite size of the ZABONC. Hence synthesized ZABONC can be used as a shielding material in EM (X/γ) radiation shielding.

• Journal of Ginseng Research
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• v.40 no.2
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• pp.185-195
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• 2016

Background: To investigate the effect of pectinase-treated Panax ginseng (GINST) in cellular and male subfertility animal models. Methods: Hydrogen peroxide ($H_2O_2$)-induced mouse spermatocyte GC-2spd cells were used as an in vitro model. Cell viability was measured using MTT assay. For the in vivo study, GINST (200 mg/kg) mixed with a regular pellet diet was administered orally for 4 mo, and the changes in the mRNA and protein expression level of antioxidative and spermatogenic genes in young and aged control rats were compared using real-time reverse transcription polymerase chain reaction and western blotting. Results: GINST treatment ($50{\mu}g/mL$, $100{\mu}g/mL$, and $200{\mu}g/mL$) significantly (p < 0.05) inhibited the $H_2O_2$-induced ($200{\mu}M$) cytotoxicity in GC-2spd cells. Furthermore, GINST ($50{\mu}g/mL$ and $100{\mu}g/mL$) significantly (p < 0.05) ameliorated the $H_2O_2$-induced decrease in the expression level of antioxidant enzymes (peroxiredoxin 3 and 4, glutathione S-transferase m5, and glutathione peroxidase 4), spermatogenesis-related protein such as inhibin-${\alpha}$, and specific sex hormone receptors (androgen receptor, luteinizing hormone receptor, and follicle-stimulating hormone receptor) in GC-2spd cells. Similarly, the altered expression level of the above mentioned genes and of spermatogenesis-related nectin-2 and cAMP response element-binding protein in aged rat testes was ameliorated with GINST (200 mg/kg) treatment. Taken together, GINST attenuated $H_2O_2$-induced oxidative stress in GC-2 cells and modulated the expression of antioxidant-related genes and of spermatogenic-related proteins and sex hormone receptors in aged rats. Conclusion: GINST may be a potential natural agent for the protection against or treatment of oxidative stress-induced male subfertility and aging-induced male subfertility.