• Title/Summary/Keyword: Nuclear morphology

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Two Freshwater Cryptomonads New to Korea: Cryptomonas marssonii and C. pyrenoidifera

  • Kim, Jee-Hwan;Boo , Sung-Min;Shin, Woong-Ghi
    • ALGAE
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    • v.22 no.3
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    • pp.147-152
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    • 2007
  • We described two brownish freshwater Cryptomonas species, C. marssonii Skuja and C. pyrenoidifera Geitler as first records in Korea. The identification was based on light microscopy, scanning electron microscopy, and nuclear SSU rDNA sequences analysis. Cryptomonas marssonii is characterized by its sigmoid shape with a sharply pointed and dorsally curved antapex, dorso-ventrally flattened cell, two lateral plastids without pyrenoid, and its dimension of 18-25 μm in length and 8-13 μm in width. Cryptomonas pyrenoidifera is characterized by ovoid to elliptical shape with a partially twisted or rounded antapex, dorso-ventrally biconvex cell, lateral plastids with two pyrenoids, and the dimensions of 15-22 μm in length and 10-14 μm in width. Nuclear SSU rDNA sequences between C. marssonii WCK01 from Korea and CCAC0086 from Gernmay, and between C. pyrenoidifera WCK02 from Korea and CCMP152 from Australia were identical, respectively.

Fabrication and Characterization of Carbon-Coated Cu Nanopowders by Pulsed Wire Evaporation Method (전기선폭발법에 의해 카본 코팅된 Cu 나노분말의 제조 및 특성 연구)

  • Lee, H.M.;Park, J.H.;Hong, S.M.;Uhm, Y.R.;Rhee, C.K.
    • Journal of Powder Materials
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    • v.16 no.4
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    • pp.243-248
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    • 2009
  • Carbon-coated Cu nanopowders with core/shell structure have been successfully fabricated by pulsed wire evaporation (PWE) method, in which a mixed gas of Ar/$CH_4$ (10 vol.%) was used as an ambient gas. The characterization of the samples was carried out using x-ray diffraction (XRD), scanning electron microscope (SEM), and high resolution transmission electron microscope (HRTEM). It was found that the nanoparticles show a spherical morphology with the size ranging of 10-40 nm and are covered with graphite layers of 2-4 nm. When oxygen-passivated Cu nanopowders were annealed under flowing argon gas (600 and 800$^{\circ}C$), the crystallinity of $Cu_2O$ phase and the particle size gradually increased. On the other hand, carbon-coated Cu nanopowders remained similar to as-prepared case with no additional oxide or carbide phases even after the annealing, indicating that the metal nanoparticles are well protected by the carbon-coating layers.

Morphometry of Nuclei in Adenocarcinoma of Prostate (전립선 선암의 화상 계측에 관한 연구)

  • Park, Hye-Rim;Chae, Seung-Wan;Sohn, Jin-Hee;Park, Young-Euy
    • The Korean Journal of Cytopathology
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    • v.6 no.2
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    • pp.99-105
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    • 1995
  • Morphometry of nuclei of the benign and malignant prostatic lesions was performed to study the relationship between nuclear size and shape and the prognosis of prostatic adenocarcinoma fifty one cases of prostatic adenocarcinoma and 13 cases of benign prostatic hyperplasia were included to evaluate area, perimeter, Dmax, Dmin, and 5 form factors of the nuclei by image analyzer(Zeiss Ibas 2000) using hematoxylin-eosin stained slides. All analytic factors of nuclear size and shape were significantly different between benign lesions and adenocarcinomas. Increased nuclear size was associated with nuclear irregularity, presence of metastasis, advanced clinical stage, and high Gleason's grade and score of prostatic adenocarcinoma. On Kaplan-Meier method, survival was decreased with older age, no hormonal treatment, stage D, high Gleason's grade and stage as well as with larger size and irregular shape of the nuclei in conclusion, morphometry of nuclei of the prostate can be a helpful tool to differentiate between be nign and malignant lesions. Nuclear morphology is thought to be associated with prognosis of prostatic adenocarcinoma.

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Nuclear star formation in galaxies due to non-axisymmetric bulges

  • Kim, Eunbin;Kim, Sungsoo S.;Lee, Gwang-Ho;Lee, Myung Gyoon;Grijs, Richard De;Choi, Yun-Young
    • The Bulletin of The Korean Astronomical Society
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    • v.39 no.1
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    • pp.38.1-38.1
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    • 2014
  • A non-axisymmetric mass distribution in the galactic bulge (or bar) causes gas flow from the disk to the nuclear region, inducing intense star formation in the nucleus. We investigate the relation between the ellipticity of the bulge and the presence of a nuclear starburst by using a volume-limited sample of galaxies. We use 1,680 spiral galaxies with Mr < -19.5 at 0.02 <= z < 0.05 in the Sloan Digital Sky Survey Data Release 7. We find that the occurrence of nuclear starburst has a moderate correlation with bulge ellipticity in intermediate-type spiral galaxies (morphology classes Sab~Sb) in low galaxy number density environments. In high galaxy number density environments, close encounters and mergers between galaxies can cause gas inflow to the nuclear region even without the presence of non-axisymmetric bulges.

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Design and Optimization for the Windowless Target of the China Nuclear Waste Transmutation Reactor

  • Cheng, Desheng;Wang, Weihua;Yang, Shijun;Deng, Haifei;Wang, Rongfei;Wang, Binjun
    • Nuclear Engineering and Technology
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    • v.48 no.2
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    • pp.360-367
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    • 2016
  • A windowless spallation target can provide a neutron source and maintain neutron chain reaction for a subcritical reactor, and is a key component of China's nuclear waste transmutation of coupling accelerator and subcritical reactor. The main issue of the windowless target design is to form a stable and controllable free surface that can ensure that energy spectrum distribution is acquired for the neutron physical design when the high energy proton beam beats the lead-bismuth eutectic in the spallation target area. In this study, morphology and flow characteristics of the free surface of the windowless target were analyzed through the volume of fluid model using computational fluid dynamics simulation, and the results show that the outlet cross section size of the target is the key to form a stable and controllable free surface, as well as the outlet with an arc transition. The optimization parameter of the target design, in which the radius of outlet cross section is $60{\pm}1mm$, is verified to form a stable and controllable free surface and to reduce the formation of air bubbles. This work can function as a reference for carrying out engineering design of windowless target and for verification experiments.

TRIGGERING AND ENERGETICS OF A SINGLE DROP VAPOR EXPLOSION: THE ROLE OF ENTRAPPED NON-CONDENSABLE GASES

  • Hansson, Roberta Concilio
    • Nuclear Engineering and Technology
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    • v.41 no.9
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    • pp.1215-1222
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    • 2009
  • The present work pertains to a research program to study Molten Fuel-Coolant Interactions (MFCI), which may occur in a nuclear power plant during a hypothetical severe accident. Dynamics of the hot liquid (melt) droplet and the volatile liquid (coolant) were investigated in the MISTEE (Micro-Interactions in Steam Explosion Experiments) facility by performing well-controlled, externally triggered, single-droplet experiments, using a high-speed visualization system with synchronized digital cinematography and continuous X-ray radiography. The current study is concerned with the MISTEE-NCG test campaign, in which a considerable amount of non-condensable gases (NCG) are present in the film that enfolds the molten droplet. The SHARP images for the MISTEE-NCG tests were analyzed and special attention was given to the morphology (aspect ratio) and dynamics of the air/ vapor bubble, as well as the melt drop preconditioning. Energetics of the vapor explosion (conversion ratio) were also evaluated. The MISTEE-NCG tests showed two main aspects when compared to the MISTEE test series (without entrapped air). First, analysis showed that the melt preconditioning still strongly depends on the coolant subcooling. Second, in respect to the energetics, the tests consistently showed a reduced conversion ratio compared to that of the MISTEE test series.

Uranium tetrafluoride production at pilot scale using a mercury electrode cell

  • Dides, Munir;Hernandez, Jose;Olivares, Luis
    • Nuclear Engineering and Technology
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    • v.54 no.5
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    • pp.1909-1913
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    • 2022
  • This work shows the technical feasibility to obtain uranium tetrafluoride through an electrochemical mercury cell. This technique represents a custom scaling-up methodology from our previous studies to obtain UF4 using the dropping mercury electrode cell. The UF4 products were obtained from natural UF6 gas, which was hydrolyzed to obtain a 50 g/L UO2F2 solution. The electrolysis cell was made using a mercury reservoir, to reach UF4 production rates of 1 Kg UF4/day. This custom design allowed a stable UF4 production thanks to the mercury cathode, which do not permit the accumulation of solid products in its surface. The cell was tested using current densities from 5.000 to 17.500 A/m2 and temperatures from 25 to 65 ℃. The maximum current efficiency achieved under these conditions was 80%. The UF4 powders possessed spherical morphology, with diameters between 20 and 80 ㎛. Compared to the SnCl2 precipitation, this process did not allow preferential growth of the precipitates. This improved the compaction of the UF4 - Mg powders mixtures, with densities between 3.0 and 3.5 g/cm3. The purity of the UF4 products was over 98%.

Morphology and phylogenetic relationships of Micractinium (Chlorellaceae, Trebouxiophyceae) taxa, including three new species from Antarctica

  • Chae, Hyunsik;Lim, Sooyeon;Kim, Han Soon;Choi, Han-Gu;Kim, Ji Hee
    • ALGAE
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    • v.34 no.4
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    • pp.267-275
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    • 2019
  • Three new species of the genus Micractinium were collected from five localities on the South Shetland Islands in maritime Antarctica, and their morphological and molecular characteristics were investigated. The vegetative cells are spherical to ellipsoidal and a single chloroplast is parietal with a pyrenoid. Because of their simple morphology, no conspicuous morphological characters of new species were recognized under light microscopy. However, molecular phylogenetic relationships were inferred from the concatenated small subunit rDNA, and internal transcribed spacer (ITS) sequence data indicated that the Antarctic microalgal strains are strongly allied to the well-supported genus Micractinium, including M. pusillum, the type species of the genus, and three other species in the genus. The secondary structure of ITS2 and compensatory base changes were used to identify and describe six Antarctic Micractinium strains. Based on their morphological and molecular characteristics, we characterized three new species of Micractinium: M. simplicissimum sp. nov., M. singularis sp. nov., and M. variabile sp. nov.

Phylogenetic Relationships of Soranthera ulvoidea (Chordariaceae, Phaeophyceae) on the Basis of Morphology and Molecular Data

  • Cho, Ga-Youn;Kim, Myung-Sook;Boo, Sung-Min
    • ALGAE
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    • v.20 no.2
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    • pp.91-97
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    • 2005
  • The brown algal family Chordariaceae sensu lato is a focus of taxonomy because recent studies suggest a broad concept of the family, including genera formerly classified in the Dictyosiphonales. Using morphology, plastid rbcL and nrDNA ITS sequences, we evaluated relationships of the monotyic genus Soranthera (S. ulvoidea), which has been classified in the Punctariaceae. The species occurs in Bering Sea and Aleutian Islands, Alaska to Baja California. Thalli are globose to lobed, hollow, 3-5 cm in diameter, and covered with evenly distributed sori. However, two forms within the species are recognized: f. ulvoidea for globose forms and f. difformis for lobed forms. Plastid rbcL and nuclear ITS region sequences were newly determined in samples of S. ulvoidea from the Pacific coast of the North America. We found little variations in the ITS sequences among samples of S. ulvoidea from five different locations and in the rbcL region from two different locations. These results do not support previous classification of f. ulvoidea and f. difformis within the species. All analyses of our rbcL sequence dataset show that Soranthera was placed in the Chordariaceae s.l., but more related to Botrytella than Punctaria and Asperococcus.

First Description of Petalonia zosterifolia and Scytosiphon gracilis (Scytosiphonaceae, Phaeophyceae) from Korea with Special Reference to nrDNA ITS Sequence Comparisons

  • Cho, Ga-Youn;Yang, Eun-Chan;Lee, Sang-Hee;Boo, Sung-Min
    • ALGAE
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    • v.17 no.3
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    • pp.135-144
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    • 2002
  • Scytosiphonaceae is an acetocarpalean brown algal family, that is a recent focus of synstematics and marine biodiversity. We describe Petalonia zosterifolia and Scytosiphon gracilis from Korea for the first time. P. zosterifolia occurred on the East coast, and had flat, linear and solid thalli. S. gracilis was found in Jeju, and had cylindircal to flat and hol-low thalli. However, these two species are so similar that it is difficult to identify by morphology alone. In order to determine if the nuclear DNA reveals the distinctness of both species and to know their phylogenies, the ITS region sequences were newly detrmined in 22 samples of P. zosterifolia, Scytosiphon gracilis, and other three members of the genera from Korea. We found 0.12% variation among samples of P. zosterifolia from different locations, and no variation between S. gracilis samples from diferent years, but extensive interspecific divergences (13.62-22.83%) of each species to other members in Petalonia and Scytosiphon . The ITS sequence dta consistently showed a close relationship between P. zosterifolia and S. gracilis. This result is congruent with morphology and with the published data of plastid rbc and partial nrDNA large subunit gene sequences, and suggests that P. zosterifolia and S. gracilis might have diverged from the most recent common ancestor.