• Journal of Ecology and Environment
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• v.46 no.4
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• pp.292-303
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• 2022

Background: Climate change significantly influences the geographical distribution of plant species worldwide. Selecting indicator species allows for better-informed and more effective ecosystem management in response to climate change. The Korean Peninsula is the northernmost distribution zone of warm temperate evergreen broad-leaved (WTEB) species in Northeast Asia. Considering the ecological value of these species, we evaluated the current distribution range and future suitable habitat for 13 WTEB tree species designated as climate-sensitive biological indicator species. Results: Up-to-date and accurate WTEB species distribution maps were constructed using herbarium specimens and citizen science data from the Korea Biodiversity Observation Network. Current northern limits for several species have shifted to higher latitudes compared to previous records. For example, the northern latitude limit for Stauntonia hexaphylla is higher (37° 02' N, Deokjeokdo archipelago) than that reported previously (36° 13' N). The minimum temperature of the coldest month (Bio6) is the major factor influencing species distribution. Under future climate change scenarios, suitable habitats are predicted to expand toward higher latitudes inland and along the western coastal areas. Conclusions: Our results support the suitability of WTEB trees as significant biological indicators of species' responses to warming. The findings also suggest the need for consistent monitoring of species distribution shifts. This study provides an important baseline dataset for future monitoring and management of indicator species' responses to changing climate conditions in South Korea.

• Journal of Plant Biotechnology
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• v.7 no.1
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• pp.45-50
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• 2005

To clone blue and white flower color specific genes, mRNA differential display was carried out with two different Commelina species, C. communis Linne for blue color and C. coreana Leveille for. leucantha Nakai for white color. Fifty two and 100 cDNA clones specific for blue or white flower color, respectively, were ranging from 200 to 700 bp in size. From the reverse northern blot analysis, 12 and 7 positive clones were selected for blue and white flower, respectively. These clones appear to be novel cDNAs for these Commelina plants, but not color specific. This finding was supported by the northern blot analysis. However, two clones, B18 and B19, derived from blue flowered Commelina were highly expressed than in the white Commelina species, implying that further study will be valuable. The results indicated that both mRNA display experiment and dot blot analysis may not sensitive enough to clone color-determining gene from the plant, leading to explore more advanced method, like high-density colony array study (HDCA).

• Korean Journal of Plant Taxonomy
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• v.40 no.4
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• pp.208-217
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• 2010

Crossosomatales is a recently recognized order in the rosid II clade with about 64 species in eight morphologically distinct families that have been previously classified in as many as 15 other orders. Phylogenetic relationships among the families and genera within Crossosomatales were investigated using chloroplast atpB, matK, and rbcL sequences employing maximum parsimony, maximum likelihood, and Bayesian methods. The phylogenetic framework was used to examine the patterns of morphological evolution and synapomorphies for subclades within Crossosomatales. The combined data with representative species from all genera in the order strongly supported monophyly of Crossosomatales. Strong support was found for the families in the Southern Hemisphere, in which Aphloiaceae is sister to the clade of (Geissolomataceae, (Ixerbaceae + Strasburgeriaceae)). The sister relationship between the Southern Hemisphere clade and families distributed primarily in the Northern Hemisphere was also supported. As in the previous studies, following relationships were found within the Northern Hemisphere clade: Staphyleaceae is sister to a clade of (Guamatelaceae, (Stachyuraceae + Crossosomataceae)). The pattern analysis indicates that evolutionary pattern of morphological characters is complex, requiring multiple changes within Crossosomatales. Several reproductive traits, such as inflorescence, aril, stigma, and conspicuous protrusion from pollen aperture, corroborate the molecular phylogeny.

• Korean Journal of Plant Tissue Culture
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• v.22 no.4
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• pp.195-200
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• 1995

The mammalian adenosine deaminase(ADA) gene was stably expressed in transgenic tobacco plane. The chimeric ADA gene 35S/35S/AMV/ADA/Tnos, has been constructed. This chimeric gene was introduced into the binary vector pRD400, which was thereafter mobilized into Agrobacterium tumefaiens strain MP90 harboring disarmed Ti-plasmid. The resulting strains were used to transform Nicofiana tabacum L. using the leaf disc. Incorporation of the chimeric gene into plant were confirmed by PCR and Northern blot analyses. Immunoblot analysis showed that ADA protein was successfully synthesized in the transgenic tobacco plants.

• Korean Journal of Plant Taxonomy
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• v.37 no.1
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• pp.41-59
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• 2007

This study was to establish the floristic composition of northern region in Gyeonggi-Province area($126^{\circ}45^{\prime}-127^{\circ}37^{\prime}E$, $37^{\circ}31^{\prime}-38^{\circ}17^{\prime}N$). This work was investigated from April, 1988 to October, 2004. The flora of vascular plants of northern region in Gyeonggi-Province were consisted of total 2,030 taxa; 145 families, 656 genera, 1,611 species, 7 subspecies, 320 varieties, 1 subvarieties and 91 formae. Among them, Korean endemic species were composed of 46 families, 85 genera, 84 species, 35 varieties, 1 subvarieties, and 18 formae, total 138 taxa. The rare and endangered plants based on floral region indicated by Ministry of Environment were 416 taxa; V rank species 18 taxa, IV rank species 79 taxa, III rank species 111 taxa, II rank species 80 taxa, I rank species 128 taxa. Also the naturalized plants were 114 species, correspond to 39.7% of among total 287 species appeared in South Korea. the result of PCA, Gwangju mountain chain at northern region of Gyeonggi-Province has rich and diverse vascular plants, and there are many Korean endemic, rare and endangered plants. therefore Gwangju mountain chain must be the core axis in for conserving the plant biodiversity and richness.

• The Korean Journal of Ecology
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• v.17 no.4
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• pp.513-521
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• 1994

Distribution patern of 30 species that are occurring predominantly in the mantle communities (Mantelgesellschaften) in South Korea was studied. The study was arried out by geographic and bioclimatic analysis on 368 releves obtained from the Zurich-Montpellier School's method, which involves direct analysis on the latitude, altitude, annual mean temperature and the lowest temperature of the site. Rosa multiflora and Pueraria thunbergiana which are regarded as repersentative pioneer species to the mantle community has the highest frequency, 70.1% and 60.3%, respectively. Three distribution patterns were recognized, i.e. northern type, central type and southern type, and each type was characterized by horizontal and altitudinal amplitude. Their concetrate distribution ranges on the annual mean temperature were 8~11℃, 9~12℃ and 10~13℃, respectively. It was recognized that tendencies of overlapping and continuous distribution pattern of the types and species exist. Geographically, the souther limit f the northern type is 35.5。N and the northern limit of the southern type 37.0。N. The central type is located at an coincided with the previous study in which cool-temperate forests were synchorologically indentified into northern/altimontane, certral/montane and southern/submontane type. The subsidiary knowledges from this study will provide practical information on the constructuin of the fence plant community for environmental conservation.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 1994.06a
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• pp.86-102
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• 1994

To understand the molecular structure and pathogenesis mechanism of Korean garlic viruses, we have isolate cDNA clones for garlic viruses. The partial nucleotide sequences of 24 cDNA clones were determined and that of six clones containing poly (A) tail were compared with those of other plant viruses. One of those clones, V9 has 81.8% similarity in nucleotide sequence and 93.0% in deduced amino acid sequence, respectively, to the coat protein gene for garlic mosaic virus (GMV). Northern blot analysis with the clone V9 demonstrated that the genome of GMV is 7.8 kb long and has poly (A) tail. The anti-coat protein antibody for GMV recognizes 35 kDa polypeptide which could be the coat protein of GMV from infected garlic leaf extract or virus preparation. Clone G7 has about 62% of deduced amino acid sequence identity with the members of potyvirus group. Northern blot analysis with the clone G7 demonstrated that the genome of the potyvirus I garlic is 9.0 kb long and has poly (A) tail. The third clone, S81, shows 42% amino acid identity to the potexvirus. The other clones are under the characterization. To test the possibility of producing garlic virus resistant plant, we have designed a hairpin type ribozyme to cleave V9 RNA at the middle of the coat protein gene. From the cleavage reactions in vitro with two different sizes of RNA substrates, V9SUB (144 nucleotides) and V9 RNA (1,361 nucleotides), the ribozyme can cleave V9 sequence effectively at the predicted site. To study the activity of the ribozyme in vivo, plant transformation is in progress. Further possibilities to produce garlic virus resistant plant will be discussed.

• Research in Plant Disease
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• v.29 no.2
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• pp.137-144
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• 2023

Exserohilum turcicum is a fungus that causes northern corn leaf blight (NCLB) and has deleterious effects on maize production globally. Therefore, it is prudent to mitigate the effects of NCLB using genetic diversity. The objective of this research was to assess the severity of NCLB disease on the growth and yield of various maize genotypes. A randomized complete block design field experiment, replicated three times, was conducted to evaluate the effect of E. turcicum on 10 maize hybrids. Percent disease index, plant height, and leaf area were recorded at the silk stage. Cob weight, grain fresh weight, and grain yields were determined at harvest maturity. All measured parameters were significantly different (P<0.05) between the maize hybrids. Of the 10 genotypes, four (PAN 4R-528R, PAN 4R-728BR, PAN 3R-724BR, and P1788BR) were susceptible, five (DKC74-74BR, PAN 5R-582R, PAN 5R-890R, PAN 5R-854R, and PAN 5R-590R) were moderately susceptible, and one (DKC80-40BR) was moderately resistant. DKC80-40BR exhibited greater cob weight, while DKC74-74BR was superior in all other plant growth and yield components. Interestingly, although not significant (P>0.05) and high, maize growth and yield parameters had negative correlations with disease incidence, except for grain fresh weight. Therefore, DKC80-40BR may be selected for cultivation in areas prone to NCLB to reduce maize susceptibility to the disease, while DKC74-74BR may improve crop performance. These hybrids could be considered as potential sources of resistance or tolerance to NCLB for further validation by plant breeders.

• Proceedings of the Botanical Society of Korea Conference
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• 1985.08b
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• pp.23-33
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• 1985

Plant cell culture is emerging to express bioactive foreign proteins because it has several advantages in that it is safe, economical, genetically stable and eukaryotic expression system comparing with other expression systems. However several limitations such as slow growth rate, low expression level and lack of well established down stream process need to be answered. As a preliminary approach to produce the immunologically interested molecules through the plant cell culture, we tested if granulocyte-macrophage colony stimulating factors (GM-CSFs) from both murine (mGM-CSF) and human (hGM-CSF) are produced as a biologically active form through plant cell culture. The murine and human GM-CSF genes were cloned into the plant expression vector, pBI121, and Ti-plasmid mediated transformation of tobacco leaves was conducted using Agrobacterium tumefaciens harboring both recombinant GM-CSF (rGM-CSF) genes. Cell suspension culture was established from the leaf-derived calli of transgenic tobacco plant. Northern blot analysis indicated the expression of the introduced mGM-CSF gene in both transgenic plant and cell suspension cultures. In addition, the biological activities of both murine and human GM-CSF from plant cell culture were confirmed by measuring the proliferation of the GM-CSF dependent FDC-PI and TF-1 cells, respectively.

• Journal of Plant Biology
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• v.17 no.3
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• pp.107-112
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• 1974

Leaf temperature is complicated with the microclimate and the dry matter production in a plant community. But a daily change of leaf temperature varying by the locality in plant body or plant community is not yet clear. To resolve such a question, following experiment was designed; Helianthus tuberosus L., Glycine max L., Zea mays L., Impomoea batatas Lam., and Cucurbita moschata var. toonas Makino were planted in the different sandy loam, $2m{\times}2m-quadrat$, which has a eastern, southern, western and northern edge. In each plot 17-25 plants were planted and the distances between individuals spaced uniformly. And leaf temperature were measured by MR3-C type thermistor from 14th May through 20th August. It is seems that the upper leaf is affected by solar radiation, the lower leaf by released heat from the earth and the middle leaf by the conditions of both zones. Measuring the temperature of a leaf that is on terminal, central, left and right margins and base part, temperature of control plant in a leaf was sloped during about two hours from noon. It is noticeable as a "noon sleeping" phenomenon.henomenon.