Browse > Article

Approach for Cloning and Characterization of Blue/White Flower Color Specific cDNA Clones from Two Commelina Species  

Lee Gunho (Genome Research Institute, Chungnam National University)
Yeon Mooshik (Genome Research Institute, Chungnam National University)
Hur Yoonkang (Genome Research Institute, Chungnam National University)
Publication Information
Journal of Plant Biotechnology / v.7, no.1, 2005 , pp. 45-50 More about this Journal
Abstract
To clone blue and white flower color specific genes, mRNA differential display was carried out with two different Commelina species, C. communis Linne for blue color and C. coreana Leveille for. leucantha Nakai for white color. Fifty two and 100 cDNA clones specific for blue or white flower color, respectively, were ranging from 200 to 700 bp in size. From the reverse northern blot analysis, 12 and 7 positive clones were selected for blue and white flower, respectively. These clones appear to be novel cDNAs for these Commelina plants, but not color specific. This finding was supported by the northern blot analysis. However, two clones, B18 and B19, derived from blue flowered Commelina were highly expressed than in the white Commelina species, implying that further study will be valuable. The results indicated that both mRNA display experiment and dot blot analysis may not sensitive enough to clone color-determining gene from the plant, leading to explore more advanced method, like high-density colony array study (HDCA).
Keywords
Commelina species; mRNA differential display; flower color;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Chen IP, Haehnel U, Altschmied L, Schubert I, Puchta H (2003) The transcriptional response of Arabidopsis to genotoxic stress - a high-density colony array study (HDCA). Plant J 35: 771-786   DOI   ScienceOn
2 Fukusaki E, Kawasaki K, Kajiyama S, An CI, Suzuki K, Tanaka Y, Kobayashi A (2004) Flower color modulations of Torenia hybrida by downregulation of chalcone synthase genes with RNA interference. J Biotechnol 111: 229-240   DOI   ScienceOn
3 Sambrook J, Russell OW (2001) Molecular Cloning: A Laboratory Manual, 3rded., Cold Spring Harbor Press, Cold Spring Harbor, New York
4 Yabuya T, Nakamura M, Iwashita T, Yamaguchi M, Takehara T (1997) Anthocyanin-flavone copigmentation in bluish purple flowers of Japanese garden iris (Iris ensata Thunb.). Euphytica 98: 163-167   DOI   ScienceOn
5 Fukui Y, Tanaka Y, Kusumi T, Iwashita T, Nomoto K (2003) A rationale for the shift in colour towards blue in transgenic carnation flowers expressing the flavonoid 3' ,5' -hydroxylase gene. Phytochemistry 63: 15-23   DOI   ScienceOn
6 Yoshida K, Toyama Y, Kameda K, Kondo T (2000) Contribution of each caffeoyl residue of the pigment molecule of gentiodelphin to blue color development. Phytochemistry 54: 85-92   DOI   ScienceOn
7 De Vetten N, ter Horst J, van Schaik HP, de Boer A, Mol J, Koes R (1999) A cytochrome $b_5$ is required for full activity of flavonoid 3',5' -hydroxylase, a cytochrome P450 involved in the formation of blue flower colors. Proc Natl Acad Sci 96: 778-783   DOI
8 Holton TA, Brugllera F, Tanaka Y (1993b) Cloning and expression of flavonol synthases from Petunia hybrida. Plant J 4: 1003-1010   DOI   ScienceOn
9 Yoshida K (1995) Cause of blue petal color. Nature 373: 291   DOI   ScienceOn
10 Fujiwara H, Tanaka Y, Fukui Y, Nakao M, Ashikari T, Kusumi T (1997) Anthocyanin 5-aromatic acyltrasferase from Gentiana triflora, purification, characterization and its role in anthocyanin biosynthesis. Eur J Biochem 249: 45-51   DOI   ScienceOn
11 Goto T Kondo T (1991) Structure and molecular stacking of anthocyan ins-flower color variation. Angrew Chem Int Ed Engl 30: 17-33   DOI
12 Yoshida K, Toyama-Kato Y, Kameda K, Kondo T (2003) Sepal color variation of Hydrangea macrophylla and vacuolar pH measured with a proton-selective microelectrode Plant Cell Physiol 44: 262-268   DOI   ScienceOn
13 Holton TA, Brugliera F, Lester DR, Tanaka Y, Hyland CD, Menting JGT, Lu CY, Farcy E Stevenson TW, Cornish EC (1993a) Cloning and expression of cytochrome P450 genes controlling flower colour. Nature 366: 276-279   DOI   ScienceOn
14 Tanaka Y, Tsuda S, Kusumi T (1998) Metabolic engineering to modify flower color. Plant Cell Physiol 137: 1119-1126
15 Okinaka Y, Shimada Y, Nakano-Shimada R, Ohbayashi M, Kiyokawa S, Kikuchi Y (2003) Selective accumulation of delphinidin derivatives in tobacco using a putative flavonoid 3' ,5' -hydroxylase cDNA from Campanula medium. Biosci Biotechnol Biochem 67: 161-165   DOI   ScienceOn
16 Fujiwara H, Tanaka Y, Yonekura-Sakakibara K, FukuchiMizutani M, Nakao M, Fukui Y, Yamaguchi M, Ashikari T, Kusumi T (1998) cDNA cloning, gene expression and subcellular localization of anthocyanin 5-aromatic acyltransferase from Genetiana triflora. Plant J 16: 421-431   DOI   ScienceOn
17 Hrabak EM, Chan CWM, Gribskov M, Harper JF, Choi JH, Halford H, Kudla J, Luan S, Nimmo HG, Sussman MR, Thomas M, Walker-Simmons K, Zhu JK, Harmon AC (2003) The Arabidopsis CDPK-SnRK superfamily of protein kinases. Plant Physiol 132: 666-680   DOI   ScienceOn
18 Mol J, Cornish E, Mason J, Koes R (1999) Novel coloured flowers. Curr Opin Biotechnol 10: 198-201   DOI   ScienceOn
19 Mori S, Kobayashi H, Hoshi Y, Kondo M, Nakano M (2004) Heterologous expression of the flavonoid 3', 5' -hydroxylase gene of Vinca major alters flower color in transgenic Petunia hybirida. Plant Cell Physiol 22: 415-421
20 Adia R, Yoshida K, Kondo T, Kishimoto S, Shibata M (2000) Copigmentation gives bluer flower on transgenic torenia plants with the antisense dihydroflavonol-4-reductase gene. Plant Sci 160: 49-56   DOI   ScienceOn
21 Shirley BW (1996) Flavonoid biosynthesis: 'new' functions for an 'old' pathway. Trends Plant Sci 1: 377-382