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Transgenic lettuce (Lactuca sativa L.) with increased vitamin C levels using GalUR gene (GalUR 유전자를 이용한 비타민 C 증대 상추 (Lactuca sativa L.) 형질전환체 개발)

  • Lim, Mi-Young;Cho, Yi-Nam;Chae, Won-Ki;Park, Young-Soo;Min, Byung-Whan;Harn, Chee-Hark
    • Journal of Plant Biotechnology
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    • v.35 no.2
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    • pp.115-120
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    • 2008
  • L-Ascorbic acid (vitamin C) in vegetables is an essential component of human nutrition. The objective is to transform lettuce (Lactuca sativa L.) with GalUR gene that is involved in the vitamin C biosynthesis. The cotyledons of Hwoahong (Nongwoo Bio Co.) were used to induce the callus and shoot under the selection media with MS + 30 g/L Sucrose + 0.5 mg/L BAP + 0.1 mg/L NAA + 100 mg/L kanamycin + 200 mg/L lilacillin, pH 5.2. The shoot was developed from the cut side of the explants after 3 weeks on the selection media. We successfully transformed the lettuce with GaIUR gene and analyzed the levels of vitamin C. We found that some of the lettuce transgenic lines contained higher levels of vitamin C compared with the normal one (non-transformed). Especially, some of $T_1$ lettuces inserted by GalUR showed about $3{\sim}4$ times higher content of vitamin C compared to the non-transformed lettuce. This data support the previously work performed with GLOase transgenic $T_1$ lettuces from which several times higher content of vitamin C were identified. The $T_2$ lettuces with high content of vitamin C have been selected for further analysis.

The effects of Injinhotang-sihosogantang-gagambang on liver injury (인진호탕합시호소간탕가감방(茵陳蒿湯合柴胡疏肝湯加減方)이 간손상(肝損傷)에 미치는 영향(影響))

  • Kim Tae-Sik;Kang Jae-Chun
    • Journal of Society of Preventive Korean Medicine
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    • v.3 no.2
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    • pp.183-210
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    • 1999
  • In order to investigate the protective effect of Injinhotanghapsihosogantang-gagambang on the liver injury of rats induced by $CCl_4$ and d-galactosamine, the serum transaminase(GOT&GPT) alkaline phosphatase(ALP), lactic dehydrogenase(LDH) for enzyme activities, triglyceride for serum component, liver weight and glutathione S-transferase(GST), Superoxide dimutase(SOD) were measured. All animals were divided into 5 groups, those were normal group(untreated), control group(treated with vehicle 0.9% Saline solution), sample I group(10mg/kg administrated), sample II group(30mg/kg administrated), Silymarin 200 administrated group. The results were as follows: 1. The inhibitory effects of the serum GOT activities in rats induced by $CCl_4$ were noted in both sample I (p<0.001) and sample II group(p<0.001). In serum GPT activities, sample I (p<0.01) and sample II group(p<0.01). 2. The inhibitory effects of the serum LDH activities in rats induced by $CCl_4$ were noted in both sample I (p<0.001) and sample II group(p<0.001). 3. The increased effects of the serum ALP activities in rats induced by $CCl_4$ were not recognized. 4. The inhibitory effects of the serum triglyceride content level in rats induced by $CCl_4$ were inhibited in only sample II group(p<0.05). 5. The increased effects of the liver weight level in rats induced by $CCl_4$ were inhibited in both sample I (p<0.05) and sample II group(p<0.05). 6. The inhibitory effects of the GST activities in rats induced by $CCl_4$ were not recognized. In SOD activities, both sample I (p<0.05) and sample II group(p<0.001) showed the inthbitory effects. 7. The inhibitory effects of in the serum GOT, GPT activities in rats induced by d-galactosamine were not recognized. 8. The increases of the serum LDH level in rats induced by d-galactosamine were noted in both sample I (p<0.01) and sample II group(p<0.001). 9. The inhibitory of the serum triglyceride content level in rats induced d-galactosamine were noted in only sample II group(p<0.05). According to the above results, it is considered that Injinhotanghapsihosogantang-gagambang has protective effect against liver injury in rats induced by $CCl_4$ and d-galactosamine.

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A Report for the Quaternary Gaegok 6 Fault Developed in the Mid-eastern Part of Ulsan Fault Zone, Korea (울산단층대 중동부에 발달하는 제4기 개곡 6단층에 대한 보고)

  • Ryoo, Chung-Ryul
    • Economic and Environmental Geology
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    • v.42 no.6
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    • pp.635-643
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    • 2009
  • In this paper, a Quaternary fault is described, which is developed in the mid-eastern part of Ulsan Fault Zone, near the southern Gaegok-ri, Oedong-eub, Gyeongju, Korea. The Gaegok 6 fault is developed along the contact between Early Tertiary granite and Quaternary gravel deposit overlying unconformably the granite. The fault strikes $N02^{\circ}{\sim}22^{\circ}E$ and dips $45^{\circ}{\sim}80^{\circ}$ to the west. This fault has a 30~50 cm wide cataclastic shear zone with gouge zone, mixed with Quaternary sediments and fault breccia of granite. In the main Quaternary fault plane, the orientation of striation is $17^{\circ}$, $356^{\circ}$, indicating a dextral strike-slip faulting with some normal component. There is another striation ($78^{\circ}$, $278^{\circ}$ and $43^{\circ}$, $270^{\circ}$) with reverse-slip sense, developed on the subsidiary plane which cuts the main Quaternary fault plane. In brief, the fault has been developed between the granite in the western part and the Quaternary gravel deposit in the eastern part. The western block of fault is uplifted. The striations and movement senses of faults indicate multiple compressional stages in this region. The fault has a similar orientation, westward dipping geometric pattern, and reverse sensed kinematic pattern with Gaegok 1 fault developed in the north. Thus, the Gaegok 6 fault is probably a southern extension of Gaegok 1 fault.

Experimental Research of Lost-wax Casting: Gilt-Bronze Pensive Bodhisattva (밀랍주조법을 활용한 청동반가사유상 복원 연구)

  • Yun, Yong-Hyun;Cho, Nam-Chul;Doh, Jung-Mann
    • Journal of Conservation Science
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    • v.36 no.2
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    • pp.123-142
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    • 2020
  • We attempted an experimental study of lost-wax casting to reconstruct the Gilt-bronze pensive Bodhisattva; The main object we aimed to reconstruct was the Korean national treasure No.83, Gilt-bronze pensive Bodhisattva(Maitreya); thus, we scientifically analyzed and measured the alloy ratio and casting method. Initially, the alloy ratio we used during the preliminary experiment was Cu:Sn:Pb = 95.5:4.0:0.5; we then increased tin and lead by 2.5% to consider vaporization during the main experiment. After applying lost-wax casting, we realized that 30% rosin contributed to proper hardness after the wax hardness experiment. The microstructure revealed normal casting character(α-Cu, δ), and the results of the chemical analysis are identical to those of previous studies. The analysis of the mold suggests the presence of quartz powder for structural stability and fire-resistance along with other organic materials whose contribution is still unknown. We expect that our research will serve to provide basic data for advanced studies in the future.

OPTIMAL DEELECTION OF EARTH-CROSSING OBJECT USING A THREE-DIMENSIONAL SINGLE IMPULSE (3차원에서의 순간적인 속도변화에 의한 ECO의 최적궤도변경)

  • Mihn, Byeong-Hee;Park, Sang-Young;Roh, Kyoung-Min;Choi, Kyu-Hong;Moon, Hong-Kyu
    • Journal of Astronomy and Space Sciences
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    • v.22 no.3
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    • pp.249-262
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    • 2005
  • Optimization problems are formulated to calculate optimal impulses for deflecting Earth-Crossing Objects using a Nonlinear Programming. This formulation allows us to analyze the velocity changes in normal direction to the celestial body's orbital plane, which is neglected in many previous studies. The constrained optimization in the three-dimensional space is based on a patched conic method including the Earth's gravitational effects, and yields impulsive ${\Delta}V$ to deflect the target's orbit. The optimal solution is dependent on relative positions and velocities between the Earth and the Earth-crossing objects, and can be represented by optimal magnitude and angle of ${\Delta}V $ as a functions of a impulse time. The perpendicular component of ${\Delta}V $ to the orbit plane can sometimes play un-negligible role as the impulse time approaches the impact time. The optimal ${\Delta}V $ is increased when the original orbit of Earth-crossing object is more similar to the Earth's orbit, and is also exponentially increased as the impulse time reaches to the impact time. The analyses performed in present paper can be used to the deflection missions in the future.

Development of Software Correlator for KJJVC (한일공동VLBI상관기를 위한 소프트웨어 상관기의 개발)

  • Yeom, J.H.;Oh, S.J.;Roh, D.G.;Kang, Y.W.;Park, S.Y.;Lee, C.H.;Chung, H.S.
    • Journal of Astronomy and Space Sciences
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    • v.26 no.4
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    • pp.567-588
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    • 2009
  • Korea-Japan Joint VLBI Correlator (KJJVC) is being developed by collaborating KASI (Korea Astronomy and Space Science Institute), Korea, and NAOJ(National Observatory of Japan), Japan. In early 2010, KJJVC will work in normal operation. In this study, we developed the software correlator which is based on VCS (VLBI Correlation Subsystem) hardware specification as the core component of KJJVC. The main specification of software correlator is 8 Gbps, 8192 output channels, and 262,144-points FFT (Fast Fourier Transform) function same as VCS. And the functional algorithm which is same as specification of VCS and arithmetic register are adopted in this software correlator. To verify the performance of developed software correlator, the correlation experiments were carried out using the spectral line and continuum sources which were observed by VERA (VLBI Exploration of Radio Astrometry), NAOJ. And the experimental results were compared to the output of Mitaka FX correlator by referring spectrum shape, phase rate, and fringe detection and so on. Through the experimental results, we confirmed that the correlation results of software correlator are the same as Mitaka FX correlator and verified the effectiveness of it. In future, we expect that the developed software correlator will be the possible software correlator of KVN (Korean VLBI Network) with KJJVC by introducing the correlation post-processing and modifying the user interface as like GUI (Graphic User Interface).

Anti-carcinogenic effects of non-polar components containing licochalcone A in roasted licorice root

  • Park, So Young;Kim, Eun Ji;Choi, Hyun Ju;Seon, Mi Ra;Lim, Soon Sung;Kang, Young-Hee;Choi, Myung-Sook;Lee, Ki Won;Yoon Park, Jung Han
    • Nutrition Research and Practice
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    • v.8 no.3
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    • pp.257-266
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    • 2014
  • BACKGROUND/OBJECTIVE: Licorice has been shown to possess cancer chemopreventive effects. However, glycyrrhizin, a major component in licorice, was found to interfere with steroid metabolism and cause edema and hypertension. The roasting process of licorice modifies the chemical composition and converts glycyrrhizin to glycyrrhetinic acid. The purpose of this study was to examine the anti-carcinogenic effects of the ethanol extract of roasted licorice (EERL) and to identify the active compound in EERL. MATERIALS/METHODS: Ethanol and aqueous extracts of roasted and un-roasted licorice were prepared. The active fraction was separated from the methylene chloride (MC)-soluble fraction of EERL and the structure of the purified compound was determined by nuclear magnetic resonance spectroscopy. The anti-carcinogenic effects of licorice extracts and licochalcone A was evaluated using a MTT assay, Western blot, flow cytometry, and two-stage skin carcinogenesis model. RESULTS: EERL was determined to be more potent and efficacious than the ethanol extract of un-roasted licorice in inhibiting the growth of DU145 and MLL prostate cancer cells, as well as HT-29 colon cancer cells. The aqueous extracts of un-roasted and roasted licorice showed minimal effects on cell growth. EERL potently inhibited growth of MCF-7 and MDA-MB-231 breast, B16-F10 melanoma, and A375 and A2058 skin cancer cells, whereas EERL slightly stimulated the growth of normal IEC-6 intestinal epithelial cells and CCD118SK fibroblasts. The MC-soluble fraction was more efficacious than EERL in inhibiting DU145 cell growth. Licochalcone A was isolated from the MC fraction and identified as the active compound of EERL. Both EERL and licochalcone A induced apoptosis of DU145 cells. EERL potently inhibited chemically-induced skin papilloma formation in mice. CONCLUSIONS: Non-polar compounds in EERL exert potent anti-carcinogenic effects, and that roasted rather than un-roasted licorice should be favored as a cancer preventive agent, whether being used as an additive to food or medicine preparations.

Study on the Realities in Seoul Subway Crimes: Criminal Psychology (서울지하철 범죄 실태에 대한 범죄심리학적 연구)

  • Rim, Sang-Gon
    • Korean Security Journal
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    • no.7
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    • pp.233-285
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    • 2004
  • ‘Defined legally as a violation of law' (Sutherland, E. H. Principles of Criminology, Lippincott, Chicago, 1939) Crime within the group is essentially and primarily antisocial in that the criminal who is welfare of his group acts instead against it and breaks the principles of social solidarity not merely by not doing what these principles prescribe, but by doing something exactly opposites. Any program set up to attack crime and delinquent behavior at their sources. A program of his nature needs the constant and comprehensive collaboration of psychiatrists, social works, educations, lawmakers, and public officials, since crime is a social problem and it should be treated as such. Some crime preventives which should be mentioned are as follows, (1) The insurance that every child will be decently born and that his home life be socially and economically adequate; without socially mature parents the chid is handicapped at the start; thus parental education, integrated with the public school system, should be developed now. (2) A more meaningful educational program which would emphasize ideals of citizenship, moral integrity, and respect for the law and the police. (3) A periodic check made for potential delinquents throughout the public schools and treatment provided if possible; and if not, proper segregation in institutions. (4) Careful attention paid to press, movies, and radio so that crime may no longer appear to be glamorous. This can be done by women's clubs, civic bodies, and other educational groups exerting pressure on the movie syndicates and broadcasting companies to free their productions of the tawdry and lurid characteristics of crime and criminals. Aggression associated with the phallic stage of development, The child ordinarily comprehends sexual intercourse as an aggressive and sadistic act on the part of the male, and specifically on the part of the penis. Evidence that the penis is phantasied as a weapon of violence and destruction come from unconscious productions of normal adults. Limerick, for instance, often refer to the penis as square, or too large, etc., so that intercourse is dangerous and painful for the partner, This may wall be a projection of the male's own fear of coitus. A certain portion of the death-instinct always remains within the person; it is called 'primal sadism' and according to Freud is identical with masochism. 'After the chief part of it(the death instinct) has been directed outwards towards objects, there remains as a residuum within the organism the true erotogenic masochism, which on the one hand becomes a component of the libido and on the other still has the subject itself for a object.' Criminalism, compulsive-neurotic frequent repetition of criminal acts in a compulsive manner. Like most symptoms of the compulsive-neurotic, such antisocial act are closely rated to feelings of hostility and aggression, often against the father. Because these acts are symptomatic, they afford only temporary relief and are therefore repeated. One patient with compulsive-neurotic criminalism was apprehended after breaking into hardware store and stealing money. He later confessed to many similar incidents over the preceding two years. At the same time it was apparent that he stole only for the sake of stealing. He did not need the money he thus obtained and had no special plans for using it.

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Studies on Constituents of Higher Fungi of Korea (LXXIII) Antitumor Components of the Cultured Mycelia of Paxillus atrotomentosus (한국산 고등 균류의 성분 연구(제73보) -좀우단버섯 배양 균사체의 항암 성분-)

  • Kwag, Sang-Deok;Bok, Jin-Woo;Hyun, Jin-Won;Choi, Eung-Chil;Kim, Byong-Kak
    • The Korean Journal of Mycology
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    • v.20 no.3
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    • pp.240-251
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    • 1992
  • To find antitumor components from higher fungi, the cultured mycelia of Paxillus atrotomentosus were extracted with hot water. The water soluble fraction was purified and separated by DEAE-cellulose ion exchange chromatography and Sepharose CL-4B gel filtration method. The separated fractions(Fr.) were designated CR A, B, C and D. Fr. A showed the highest inhibition ratio of 68.51% among the five tractions at a dose of 20 mg/kg/day. When Fr. A was examined for immunopotentiation activity, it increased the amount of the superoxide anion from activated macrophages to 1.1 fold and the number of plaques in hemolytic plaque assay to 2.3 fold, respectively. Otherwise, it did not show direct cytotoxity in sarcoma 180. Delayed type hypersensitiyity reaction showed that the decreased footpad swelling of tumor-hearing was restored to the normal. These results indicate that antitumor activity was exerted through immunopotentiation. Its chemical analysis showed 86.36% polysaccharide, 1.52% protein and 1.64% hexosamine. The polysaccharide consisted of fucose, galactose, glucose, mannose and xylose. This component was named paxillan.

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Evaluation on Anti-Dermatophyte Effect of Larix (kaempferi) Essential Oil on the Morphological Changes of Eermatophyte Fungal Hyphae (피부사상균 균사의 형태학적 변화를 통한 일본잎갈나무 정유의 항진균 활성 효과 구명)

  • Kim, Seon-Hong;Lee, Su-Yeon;Hong, Chang-Young;Jang, Soo-Kyeong;Lee, Sung Suk;Park, Mi-Jin;Choi, In-Gyu
    • Journal of the Korean Wood Science and Technology
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    • v.41 no.3
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    • pp.247-257
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    • 2013
  • This study was to investigate the antifungal activity of Larix kaempferi essential oil against dermatophytes, Epidermophyton floccosum, Trichophyton mentagrophytes and Trichophyton rubrum. The active components of L. kaempferi against dermatophytes were determined (characterized by GC-MS), and the morphological changes of the dermatophytes exposed to the L. kaempferi essential oil were observed by electron microscope. Main component of L. kaempferi essential oil was (-)-bornyl acetate. In antifungal activity tests, MIC of L. kaempferi crude oil was 125 ppm on every fungi and 100% (agar dilution method) at more than 500 ppm. By using SEM and TEM, the fungal morphology of E. floccosum exposed to the L. kaempferi essential oil was different from that of normal hyphal morphology. Hyphae exposed to the L. kaempferi essential oil was damaged with distorted and collapsed surfaces. In addition, there were destruction and disorganization of organelles in cytoplasm and collapse of cell membrane. Active antifungal components from L. kaempferi essential oil were identified as terpene alcohol compounds like (-)-${\tau}$-muurolol, (+)-terpinen-4-ol, ${\alpha}$-terpineol, and ${\alpha}$-cadinol.