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http://dx.doi.org/10.5658/WOOD.2013.41.3.247

Evaluation on Anti-Dermatophyte Effect of Larix (kaempferi) Essential Oil on the Morphological Changes of Eermatophyte Fungal Hyphae  

Kim, Seon-Hong (Department of Forest Sciences, College of Agriculture & Life Sciences, Seoul National University)
Lee, Su-Yeon (Department of Forest Sciences, College of Agriculture & Life Sciences, Seoul National University)
Hong, Chang-Young (Department of Forest Sciences, College of Agriculture & Life Sciences, Seoul National University)
Jang, Soo-Kyeong (Department of Forest Sciences, College of Agriculture & Life Sciences, Seoul National University)
Lee, Sung Suk (Division of Wood Chemistry & Microbiology, Department of Forest Resources Utilization, Korea Forest Research Institute)
Park, Mi-Jin (Division of Wood Chemistry & Microbiology, Department of Forest Resources Utilization, Korea Forest Research Institute)
Choi, In-Gyu (Department of Forest Sciences, College of Agriculture & Life Sciences, Seoul National University)
Publication Information
Journal of the Korean Wood Science and Technology / v.41, no.3, 2013 , pp. 247-257 More about this Journal
Abstract
This study was to investigate the antifungal activity of Larix kaempferi essential oil against dermatophytes, Epidermophyton floccosum, Trichophyton mentagrophytes and Trichophyton rubrum. The active components of L. kaempferi against dermatophytes were determined (characterized by GC-MS), and the morphological changes of the dermatophytes exposed to the L. kaempferi essential oil were observed by electron microscope. Main component of L. kaempferi essential oil was (-)-bornyl acetate. In antifungal activity tests, MIC of L. kaempferi crude oil was 125 ppm on every fungi and 100% (agar dilution method) at more than 500 ppm. By using SEM and TEM, the fungal morphology of E. floccosum exposed to the L. kaempferi essential oil was different from that of normal hyphal morphology. Hyphae exposed to the L. kaempferi essential oil was damaged with distorted and collapsed surfaces. In addition, there were destruction and disorganization of organelles in cytoplasm and collapse of cell membrane. Active antifungal components from L. kaempferi essential oil were identified as terpene alcohol compounds like (-)-${\tau}$-muurolol, (+)-terpinen-4-ol, ${\alpha}$-terpineol, and ${\alpha}$-cadinol.
Keywords
Larix kaempferi; essential oil; antifungal activity; change of hyphal morphology; terpene alcohol;
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Times Cited By KSCI : 2  (Citation Analysis)
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